Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpE and Synpcc7942_1335 overlap by 4 nucleotides Synpcc7942_1335 and Synpcc7942_1336 are separated by 289 nucleotides
Synpcc7942_1334: trpE - aminodeoxychorismate synthase, subunit I, at 1,364,783 to 1,366,180
trpE
Synpcc7942_1335: Synpcc7942_1335 - probable branched-chain amino acid aminotransferase, at 1,366,177 to 1,366,971
_1335
Synpcc7942_1336: Synpcc7942_1336 - hypothetical protein, at 1,367,261 to 1,368,343
_1336
Position (kb)
1366
1367 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1365.489 kb on + strand, within trpE at 1365.881 kb on + strand, within trpE at 1366.028 kb on - strand, within trpE at 1366.097 kb on + strand at 1366.159 kb on - strand at 1366.164 kb on + strand at 1366.334 kb on + strand, within Synpcc7942_1335 at 1366.399 kb on + strand, within Synpcc7942_1335 at 1366.446 kb on - strand, within Synpcc7942_1335 at 1366.446 kb on - strand, within Synpcc7942_1335 at 1366.446 kb on - strand, within Synpcc7942_1335 at 1366.446 kb on - strand, within Synpcc7942_1335 at 1366.466 kb on + strand, within Synpcc7942_1335 at 1366.613 kb on + strand, within Synpcc7942_1335 at 1366.613 kb on + strand, within Synpcc7942_1335 at 1366.640 kb on + strand, within Synpcc7942_1335 at 1366.671 kb on + strand, within Synpcc7942_1335 at 1366.753 kb on + strand, within Synpcc7942_1335 at 1366.766 kb on - strand, within Synpcc7942_1335 at 1366.773 kb on + strand, within Synpcc7942_1335 at 1366.917 kb on + strand at 1366.917 kb on + strand at 1366.935 kb on - strand at 1366.968 kb on - strand at 1367.019 kb on + strand at 1367.020 kb on + strand at 1367.027 kb on - strand at 1367.068 kb on + strand at 1367.086 kb on - strand at 1367.122 kb on - strand at 1367.207 kb on + strand at 1367.215 kb on - strand at 1367.225 kb on - strand at 1367.225 kb on - strand at 1367.226 kb on + strand at 1367.253 kb on + strand at 1367.296 kb on - strand at 1367.306 kb on - strand at 1367.306 kb on - strand at 1367.323 kb on + strand at 1367.354 kb on + strand at 1367.362 kb on + strand at 1367.439 kb on + strand, within Synpcc7942_1336 at 1367.458 kb on + strand, within Synpcc7942_1336 at 1367.493 kb on - strand, within Synpcc7942_1336 at 1367.504 kb on + strand, within Synpcc7942_1336 at 1367.522 kb on + strand, within Synpcc7942_1336 at 1367.529 kb on + strand, within Synpcc7942_1336 at 1367.529 kb on + strand, within Synpcc7942_1336 at 1367.529 kb on + strand, within Synpcc7942_1336 at 1367.529 kb on + strand, within Synpcc7942_1336 at 1367.529 kb on + strand, within Synpcc7942_1336 at 1367.537 kb on - strand, within Synpcc7942_1336 at 1367.537 kb on - strand, within Synpcc7942_1336 at 1367.537 kb on - strand, within Synpcc7942_1336 at 1367.537 kb on - strand, within Synpcc7942_1336 at 1367.537 kb on - strand, within Synpcc7942_1336 at 1367.537 kb on - strand, within Synpcc7942_1336 at 1367.563 kb on + strand, within Synpcc7942_1336 at 1367.566 kb on - strand, within Synpcc7942_1336 at 1367.595 kb on + strand, within Synpcc7942_1336 at 1367.602 kb on - strand, within Synpcc7942_1336 at 1367.602 kb on - strand, within Synpcc7942_1336 at 1367.603 kb on - strand, within Synpcc7942_1336 at 1367.643 kb on + strand, within Synpcc7942_1336 at 1367.643 kb on + strand, within Synpcc7942_1336 at 1367.643 kb on + strand, within Synpcc7942_1336 at 1367.651 kb on - strand, within Synpcc7942_1336 at 1367.651 kb on - strand, within Synpcc7942_1336 at 1367.651 kb on - strand, within Synpcc7942_1336 at 1367.651 kb on - strand, within Synpcc7942_1336 at 1367.651 kb on - strand, within Synpcc7942_1336 at 1367.651 kb on - strand, within Synpcc7942_1336 at 1367.653 kb on + strand, within Synpcc7942_1336 at 1367.674 kb on + strand, within Synpcc7942_1336 at 1367.674 kb on + strand, within Synpcc7942_1336 at 1367.680 kb on - strand, within Synpcc7942_1336 at 1367.682 kb on - strand, within Synpcc7942_1336 at 1367.687 kb on + strand, within Synpcc7942_1336 at 1367.696 kb on + strand, within Synpcc7942_1336 at 1367.709 kb on + strand, within Synpcc7942_1336 at 1367.717 kb on - strand, within Synpcc7942_1336 at 1367.768 kb on + strand, within Synpcc7942_1336 at 1367.864 kb on + strand, within Synpcc7942_1336 at 1367.869 kb on + strand, within Synpcc7942_1336 at 1367.869 kb on + strand, within Synpcc7942_1336 at 1367.877 kb on - strand, within Synpcc7942_1336 at 1367.917 kb on + strand, within Synpcc7942_1336 at 1367.940 kb on + strand, within Synpcc7942_1336
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,365,489 + trpE Synpcc7942_1334 0.51 -2.4 1,365,881 + trpE Synpcc7942_1334 0.79 -1.6 1,366,028 - trpE Synpcc7942_1334 0.89 -2.1 1,366,097 + -2.9 1,366,159 - +0.7 1,366,164 + -0.2 1,366,334 + Synpcc7942_1335 0.20 -5.0 1,366,399 + Synpcc7942_1335 0.28 -1.2 1,366,446 - Synpcc7942_1335 0.34 -4.9 1,366,446 - Synpcc7942_1335 0.34 -3.9 1,366,446 - Synpcc7942_1335 0.34 -3.8 1,366,446 - Synpcc7942_1335 0.34 -1.6 1,366,466 + Synpcc7942_1335 0.36 -1.8 1,366,613 + Synpcc7942_1335 0.55 -2.5 1,366,613 + Synpcc7942_1335 0.55 -0.2 1,366,640 + Synpcc7942_1335 0.58 -1.7 1,366,671 + Synpcc7942_1335 0.62 -2.4 1,366,753 + Synpcc7942_1335 0.72 -2.7 1,366,766 - Synpcc7942_1335 0.74 -3.7 1,366,773 + Synpcc7942_1335 0.75 -3.9 1,366,917 + -2.8 1,366,917 + -3.5 1,366,935 - -3.3 1,366,968 - -5.2 1,367,019 + -0.1 1,367,020 + +0.8 1,367,027 - -1.7 1,367,068 + -0.4 1,367,086 - -1.1 1,367,122 - +0.6 1,367,207 + +2.1 1,367,215 - +1.3 1,367,225 - +1.1 1,367,225 - +1.4 1,367,226 + +0.8 1,367,253 + +2.6 1,367,296 - +0.0 1,367,306 - -2.5 1,367,306 - -0.9 1,367,323 + -1.5 1,367,354 + +3.2 1,367,362 + -0.1 1,367,439 + Synpcc7942_1336 0.16 +1.6 1,367,458 + Synpcc7942_1336 0.18 -0.8 1,367,493 - Synpcc7942_1336 0.21 +1.5 1,367,504 + Synpcc7942_1336 0.22 +1.6 1,367,522 + Synpcc7942_1336 0.24 -0.0 1,367,529 + Synpcc7942_1336 0.25 +1.0 1,367,529 + Synpcc7942_1336 0.25 +0.6 1,367,529 + Synpcc7942_1336 0.25 -1.2 1,367,529 + Synpcc7942_1336 0.25 +0.1 1,367,529 + Synpcc7942_1336 0.25 +2.2 1,367,537 - Synpcc7942_1336 0.25 +0.9 1,367,537 - Synpcc7942_1336 0.25 +4.1 1,367,537 - Synpcc7942_1336 0.25 -0.5 1,367,537 - Synpcc7942_1336 0.25 -0.6 1,367,537 - Synpcc7942_1336 0.25 +1.5 1,367,537 - Synpcc7942_1336 0.25 -2.9 1,367,563 + Synpcc7942_1336 0.28 -1.6 1,367,566 - Synpcc7942_1336 0.28 +0.9 1,367,595 + Synpcc7942_1336 0.31 +1.7 1,367,602 - Synpcc7942_1336 0.31 +3.9 1,367,602 - Synpcc7942_1336 0.31 -1.3 1,367,603 - Synpcc7942_1336 0.32 -1.1 1,367,643 + Synpcc7942_1336 0.35 +0.5 1,367,643 + Synpcc7942_1336 0.35 +2.7 1,367,643 + Synpcc7942_1336 0.35 -2.1 1,367,651 - Synpcc7942_1336 0.36 +0.7 1,367,651 - Synpcc7942_1336 0.36 -1.7 1,367,651 - Synpcc7942_1336 0.36 +0.7 1,367,651 - Synpcc7942_1336 0.36 +3.0 1,367,651 - Synpcc7942_1336 0.36 -1.3 1,367,651 - Synpcc7942_1336 0.36 -2.0 1,367,653 + Synpcc7942_1336 0.36 -1.0 1,367,674 + Synpcc7942_1336 0.38 +2.1 1,367,674 + Synpcc7942_1336 0.38 +3.3 1,367,680 - Synpcc7942_1336 0.39 +1.3 1,367,682 - Synpcc7942_1336 0.39 -1.1 1,367,687 + Synpcc7942_1336 0.39 +0.4 1,367,696 + Synpcc7942_1336 0.40 -0.5 1,367,709 + Synpcc7942_1336 0.41 +1.3 1,367,717 - Synpcc7942_1336 0.42 -1.1 1,367,768 + Synpcc7942_1336 0.47 -0.6 1,367,864 + Synpcc7942_1336 0.56 -0.2 1,367,869 + Synpcc7942_1336 0.56 -1.2 1,367,869 + Synpcc7942_1336 0.56 -2.9 1,367,877 - Synpcc7942_1336 0.57 -0.5 1,367,917 + Synpcc7942_1336 0.61 -1.7 1,367,940 + Synpcc7942_1336 0.63 -2.3
Or see this region's nucleotide sequence