Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1329 and Synpcc7942_1330 are separated by 2 nucleotides Synpcc7942_1330 and Synpcc7942_1331 are separated by 125 nucleotides Synpcc7942_1331 and Synpcc7942_1332 are separated by 4 nucleotides Synpcc7942_1332 and Synpcc7942_1333 overlap by 1 nucleotides Synpcc7942_1333 and trpE overlap by 4 nucleotides
Synpcc7942_1329: Synpcc7942_1329 - hypothetical protein, at 1,361,879 to 1,362,505
_1329
Synpcc7942_1330: Synpcc7942_1330 - hypothetical protein, at 1,362,508 to 1,363,164
_1330
Synpcc7942_1331: Synpcc7942_1331 - CAB/ELIP/HLIP superfamily protein, at 1,363,290 to 1,363,466
_1331
Synpcc7942_1332: Synpcc7942_1332 - hypothetical protein, at 1,363,471 to 1,364,091
_1332
Synpcc7942_1333: Synpcc7942_1333 - ExsB, at 1,364,091 to 1,364,786
_1333
Synpcc7942_1334: trpE - aminodeoxychorismate synthase, subunit I, at 1,364,783 to 1,366,180
trpE
Position (kb)
1363
1364
1365 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1362.487 kb on + strand at 1362.490 kb on - strand at 1362.497 kb on - strand at 1362.533 kb on + strand at 1362.535 kb on + strand at 1362.541 kb on - strand at 1362.543 kb on - strand at 1362.551 kb on - strand at 1362.576 kb on + strand, within Synpcc7942_1330 at 1362.576 kb on + strand, within Synpcc7942_1330 at 1362.579 kb on - strand at 1362.583 kb on - strand, within Synpcc7942_1330 at 1362.584 kb on - strand, within Synpcc7942_1330 at 1362.584 kb on - strand, within Synpcc7942_1330 at 1362.584 kb on - strand, within Synpcc7942_1330 at 1362.584 kb on - strand, within Synpcc7942_1330 at 1362.629 kb on + strand, within Synpcc7942_1330 at 1362.637 kb on - strand, within Synpcc7942_1330 at 1362.660 kb on + strand, within Synpcc7942_1330 at 1362.665 kb on + strand, within Synpcc7942_1330 at 1362.666 kb on + strand, within Synpcc7942_1330 at 1362.743 kb on - strand, within Synpcc7942_1330 at 1362.745 kb on + strand, within Synpcc7942_1330 at 1362.804 kb on - strand, within Synpcc7942_1330 at 1362.835 kb on + strand, within Synpcc7942_1330 at 1362.843 kb on + strand, within Synpcc7942_1330 at 1362.855 kb on + strand, within Synpcc7942_1330 at 1362.865 kb on + strand, within Synpcc7942_1330 at 1362.886 kb on - strand, within Synpcc7942_1330 at 1362.886 kb on - strand, within Synpcc7942_1330 at 1362.897 kb on + strand, within Synpcc7942_1330 at 1362.905 kb on - strand, within Synpcc7942_1330 at 1362.905 kb on - strand, within Synpcc7942_1330 at 1362.905 kb on - strand, within Synpcc7942_1330 at 1362.907 kb on + strand, within Synpcc7942_1330 at 1362.909 kb on + strand, within Synpcc7942_1330 at 1362.909 kb on + strand, within Synpcc7942_1330 at 1362.909 kb on + strand, within Synpcc7942_1330 at 1362.909 kb on + strand, within Synpcc7942_1330 at 1362.909 kb on + strand, within Synpcc7942_1330 at 1362.917 kb on - strand, within Synpcc7942_1330 at 1362.917 kb on - strand, within Synpcc7942_1330 at 1362.974 kb on - strand, within Synpcc7942_1330 at 1362.996 kb on - strand, within Synpcc7942_1330 at 1363.022 kb on - strand, within Synpcc7942_1330 at 1363.022 kb on - strand, within Synpcc7942_1330 at 1363.032 kb on + strand, within Synpcc7942_1330 at 1363.032 kb on + strand, within Synpcc7942_1330 at 1363.040 kb on - strand, within Synpcc7942_1330 at 1363.040 kb on - strand, within Synpcc7942_1330 at 1363.064 kb on + strand, within Synpcc7942_1330 at 1363.084 kb on + strand, within Synpcc7942_1330 at 1363.113 kb on + strand at 1363.134 kb on + strand at 1363.142 kb on - strand at 1363.149 kb on + strand at 1363.157 kb on - strand at 1363.157 kb on - strand at 1363.157 kb on - strand at 1363.157 kb on - strand at 1363.166 kb on - strand at 1363.167 kb on - strand at 1363.212 kb on - strand at 1363.250 kb on + strand at 1363.354 kb on + strand, within Synpcc7942_1331 at 1363.362 kb on - strand, within Synpcc7942_1331 at 1363.402 kb on + strand, within Synpcc7942_1331 at 1363.467 kb on + strand at 1363.469 kb on - strand at 1363.475 kb on - strand at 1363.485 kb on - strand at 1363.485 kb on - strand at 1363.490 kb on - strand at 1363.531 kb on + strand at 1363.539 kb on - strand, within Synpcc7942_1332 at 1363.552 kb on - strand, within Synpcc7942_1332 at 1363.557 kb on + strand, within Synpcc7942_1332 at 1363.589 kb on + strand, within Synpcc7942_1332 at 1363.597 kb on - strand, within Synpcc7942_1332 at 1363.616 kb on - strand, within Synpcc7942_1332 at 1363.631 kb on - strand, within Synpcc7942_1332 at 1363.668 kb on + strand, within Synpcc7942_1332 at 1363.681 kb on + strand, within Synpcc7942_1332 at 1363.692 kb on + strand, within Synpcc7942_1332 at 1363.692 kb on + strand, within Synpcc7942_1332 at 1363.700 kb on - strand, within Synpcc7942_1332 at 1363.749 kb on + strand, within Synpcc7942_1332 at 1363.770 kb on + strand, within Synpcc7942_1332 at 1363.770 kb on + strand, within Synpcc7942_1332 at 1363.771 kb on + strand, within Synpcc7942_1332 at 1363.779 kb on - strand, within Synpcc7942_1332 at 1363.802 kb on - strand, within Synpcc7942_1332 at 1363.829 kb on + strand, within Synpcc7942_1332 at 1363.873 kb on + strand, within Synpcc7942_1332 at 1363.881 kb on - strand, within Synpcc7942_1332 at 1363.938 kb on + strand, within Synpcc7942_1332 at 1363.942 kb on + strand, within Synpcc7942_1332 at 1363.985 kb on - strand, within Synpcc7942_1332 at 1363.985 kb on - strand, within Synpcc7942_1332 at 1363.989 kb on + strand, within Synpcc7942_1332 at 1364.108 kb on - strand at 1364.131 kb on - strand at 1364.145 kb on + strand at 1364.172 kb on - strand, within Synpcc7942_1333 at 1364.189 kb on + strand, within Synpcc7942_1333 at 1364.204 kb on - strand, within Synpcc7942_1333 at 1364.259 kb on - strand, within Synpcc7942_1333 at 1364.307 kb on - strand, within Synpcc7942_1333 at 1364.312 kb on - strand, within Synpcc7942_1333 at 1364.465 kb on + strand, within Synpcc7942_1333 at 1364.553 kb on + strand, within Synpcc7942_1333 at 1364.561 kb on - strand, within Synpcc7942_1333 at 1364.630 kb on - strand, within Synpcc7942_1333 at 1364.676 kb on - strand, within Synpcc7942_1333 at 1365.024 kb on + strand, within trpE
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,362,487 + -2.7 1,362,490 - +1.1 1,362,497 - +0.5 1,362,533 + +0.6 1,362,535 + -0.5 1,362,541 - -0.5 1,362,543 - +1.9 1,362,551 - +0.4 1,362,576 + Synpcc7942_1330 0.10 -2.6 1,362,576 + Synpcc7942_1330 0.10 +0.1 1,362,579 - -1.2 1,362,583 - Synpcc7942_1330 0.11 -1.1 1,362,584 - Synpcc7942_1330 0.12 +1.2 1,362,584 - Synpcc7942_1330 0.12 -2.3 1,362,584 - Synpcc7942_1330 0.12 -1.9 1,362,584 - Synpcc7942_1330 0.12 -0.3 1,362,629 + Synpcc7942_1330 0.18 -2.1 1,362,637 - Synpcc7942_1330 0.20 +3.1 1,362,660 + Synpcc7942_1330 0.23 +0.6 1,362,665 + Synpcc7942_1330 0.24 -0.3 1,362,666 + Synpcc7942_1330 0.24 +2.1 1,362,743 - Synpcc7942_1330 0.36 -0.9 1,362,745 + Synpcc7942_1330 0.36 +1.7 1,362,804 - Synpcc7942_1330 0.45 -1.2 1,362,835 + Synpcc7942_1330 0.50 -1.1 1,362,843 + Synpcc7942_1330 0.51 -2.3 1,362,855 + Synpcc7942_1330 0.53 +1.7 1,362,865 + Synpcc7942_1330 0.54 +0.8 1,362,886 - Synpcc7942_1330 0.58 -2.0 1,362,886 - Synpcc7942_1330 0.58 +1.1 1,362,897 + Synpcc7942_1330 0.59 +0.3 1,362,905 - Synpcc7942_1330 0.60 +1.8 1,362,905 - Synpcc7942_1330 0.60 +1.5 1,362,905 - Synpcc7942_1330 0.60 -0.6 1,362,907 + Synpcc7942_1330 0.61 +2.0 1,362,909 + Synpcc7942_1330 0.61 +0.1 1,362,909 + Synpcc7942_1330 0.61 -0.9 1,362,909 + Synpcc7942_1330 0.61 -1.3 1,362,909 + Synpcc7942_1330 0.61 +0.8 1,362,909 + Synpcc7942_1330 0.61 -1.1 1,362,917 - Synpcc7942_1330 0.62 +1.0 1,362,917 - Synpcc7942_1330 0.62 +1.1 1,362,974 - Synpcc7942_1330 0.71 -2.1 1,362,996 - Synpcc7942_1330 0.74 -1.5 1,363,022 - Synpcc7942_1330 0.78 +4.0 1,363,022 - Synpcc7942_1330 0.78 +3.1 1,363,032 + Synpcc7942_1330 0.80 +1.8 1,363,032 + Synpcc7942_1330 0.80 +2.8 1,363,040 - Synpcc7942_1330 0.81 +0.9 1,363,040 - Synpcc7942_1330 0.81 -1.7 1,363,064 + Synpcc7942_1330 0.85 -1.7 1,363,084 + Synpcc7942_1330 0.88 +1.2 1,363,113 + +0.7 1,363,134 + -1.7 1,363,142 - -1.4 1,363,149 + -0.6 1,363,157 - -2.2 1,363,157 - -2.1 1,363,157 - +3.3 1,363,157 - -2.4 1,363,166 - -2.5 1,363,167 - -0.1 1,363,212 - -2.7 1,363,250 + +2.1 1,363,354 + Synpcc7942_1331 0.36 -1.6 1,363,362 - Synpcc7942_1331 0.41 -1.1 1,363,402 + Synpcc7942_1331 0.63 -2.2 1,363,467 + +2.2 1,363,469 - +0.7 1,363,475 - +1.5 1,363,485 - +0.1 1,363,485 - -0.2 1,363,490 - -1.4 1,363,531 + -1.8 1,363,539 - Synpcc7942_1332 0.11 -2.8 1,363,552 - Synpcc7942_1332 0.13 -0.7 1,363,557 + Synpcc7942_1332 0.14 -1.5 1,363,589 + Synpcc7942_1332 0.19 +0.1 1,363,597 - Synpcc7942_1332 0.20 -3.0 1,363,616 - Synpcc7942_1332 0.23 -2.8 1,363,631 - Synpcc7942_1332 0.26 -0.2 1,363,668 + Synpcc7942_1332 0.32 -1.6 1,363,681 + Synpcc7942_1332 0.34 -2.9 1,363,692 + Synpcc7942_1332 0.36 -2.7 1,363,692 + Synpcc7942_1332 0.36 -1.1 1,363,700 - Synpcc7942_1332 0.37 -0.9 1,363,749 + Synpcc7942_1332 0.45 -3.4 1,363,770 + Synpcc7942_1332 0.48 -0.9 1,363,770 + Synpcc7942_1332 0.48 -3.1 1,363,771 + Synpcc7942_1332 0.48 -1.8 1,363,779 - Synpcc7942_1332 0.50 -0.2 1,363,802 - Synpcc7942_1332 0.53 -1.2 1,363,829 + Synpcc7942_1332 0.58 -1.1 1,363,873 + Synpcc7942_1332 0.65 -2.2 1,363,881 - Synpcc7942_1332 0.66 +0.3 1,363,938 + Synpcc7942_1332 0.75 -2.4 1,363,942 + Synpcc7942_1332 0.76 -0.7 1,363,985 - Synpcc7942_1332 0.83 -1.1 1,363,985 - Synpcc7942_1332 0.83 -2.4 1,363,989 + Synpcc7942_1332 0.83 -1.1 1,364,108 - -0.6 1,364,131 - -1.2 1,364,145 + -0.7 1,364,172 - Synpcc7942_1333 0.12 +0.1 1,364,189 + Synpcc7942_1333 0.14 -1.2 1,364,204 - Synpcc7942_1333 0.16 -1.8 1,364,259 - Synpcc7942_1333 0.24 -0.8 1,364,307 - Synpcc7942_1333 0.31 -0.2 1,364,312 - Synpcc7942_1333 0.32 -0.6 1,364,465 + Synpcc7942_1333 0.54 -1.2 1,364,553 + Synpcc7942_1333 0.66 -1.4 1,364,561 - Synpcc7942_1333 0.68 -2.6 1,364,630 - Synpcc7942_1333 0.77 -2.3 1,364,676 - Synpcc7942_1333 0.84 -1.6 1,365,024 + trpE Synpcc7942_1334 0.17 -4.1
Or see this region's nucleotide sequence