Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1305 and Synpcc7942_1306 are separated by 77 nucleotides Synpcc7942_1306 and Synpcc7942_1307 are separated by 98 nucleotides Synpcc7942_1307 and trpS are separated by 12 nucleotides
Synpcc7942_1305: Synpcc7942_1305 - ATPase, at 1,331,834 to 1,333,444
_1305
Synpcc7942_1306: Synpcc7942_1306 - hypothetical protein, at 1,333,522 to 1,334,082
_1306
Synpcc7942_1307: Synpcc7942_1307 - putative ABC-2 type transport system permease protein, at 1,334,181 to 1,334,996
_1307
Synpcc7942_1308: trpS - tryptophanyl-tRNA synthetase, at 1,335,009 to 1,336,076
trpS
Position (kb)
1334
1335 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1333.201 kb on + strand, within Synpcc7942_1305 at 1333.219 kb on + strand, within Synpcc7942_1305 at 1333.222 kb on - strand, within Synpcc7942_1305 at 1333.222 kb on - strand, within Synpcc7942_1305 at 1333.222 kb on - strand, within Synpcc7942_1305 at 1333.231 kb on + strand, within Synpcc7942_1305 at 1333.239 kb on - strand, within Synpcc7942_1305 at 1333.253 kb on - strand, within Synpcc7942_1305 at 1333.301 kb on + strand at 1333.303 kb on - strand at 1333.311 kb on - strand at 1333.340 kb on + strand at 1333.348 kb on - strand at 1333.357 kb on + strand at 1333.376 kb on - strand at 1333.401 kb on - strand at 1333.431 kb on - strand at 1333.509 kb on - strand at 1334.096 kb on + strand at 1334.120 kb on + strand at 1334.185 kb on - strand at 1334.235 kb on + strand at 1334.261 kb on - strand at 1334.283 kb on - strand, within Synpcc7942_1307 at 1334.283 kb on - strand, within Synpcc7942_1307 at 1334.283 kb on - strand, within Synpcc7942_1307 at 1334.283 kb on - strand, within Synpcc7942_1307 at 1334.295 kb on - strand, within Synpcc7942_1307 at 1334.310 kb on + strand, within Synpcc7942_1307 at 1334.310 kb on + strand, within Synpcc7942_1307 at 1334.310 kb on + strand, within Synpcc7942_1307 at 1334.313 kb on - strand, within Synpcc7942_1307 at 1334.318 kb on - strand, within Synpcc7942_1307 at 1334.318 kb on - strand, within Synpcc7942_1307 at 1334.327 kb on + strand, within Synpcc7942_1307 at 1334.352 kb on + strand, within Synpcc7942_1307 at 1334.352 kb on + strand, within Synpcc7942_1307 at 1334.352 kb on + strand, within Synpcc7942_1307 at 1334.352 kb on + strand, within Synpcc7942_1307 at 1334.352 kb on + strand, within Synpcc7942_1307 at 1334.354 kb on + strand, within Synpcc7942_1307 at 1334.360 kb on - strand, within Synpcc7942_1307 at 1334.360 kb on - strand, within Synpcc7942_1307 at 1334.377 kb on + strand, within Synpcc7942_1307 at 1334.385 kb on - strand, within Synpcc7942_1307 at 1334.394 kb on + strand, within Synpcc7942_1307 at 1334.441 kb on + strand, within Synpcc7942_1307 at 1334.459 kb on - strand, within Synpcc7942_1307 at 1334.466 kb on - strand at 1334.478 kb on - strand, within Synpcc7942_1307 at 1334.514 kb on + strand, within Synpcc7942_1307 at 1334.523 kb on + strand, within Synpcc7942_1307 at 1334.536 kb on - strand, within Synpcc7942_1307 at 1334.536 kb on - strand at 1334.604 kb on - strand, within Synpcc7942_1307 at 1334.645 kb on - strand, within Synpcc7942_1307 at 1334.655 kb on + strand, within Synpcc7942_1307 at 1334.726 kb on + strand, within Synpcc7942_1307 at 1334.727 kb on + strand, within Synpcc7942_1307 at 1334.727 kb on + strand, within Synpcc7942_1307 at 1334.762 kb on + strand, within Synpcc7942_1307 at 1334.778 kb on + strand, within Synpcc7942_1307 at 1334.783 kb on + strand, within Synpcc7942_1307 at 1334.800 kb on + strand, within Synpcc7942_1307 at 1334.853 kb on + strand, within Synpcc7942_1307 at 1334.861 kb on - strand, within Synpcc7942_1307 at 1334.861 kb on - strand, within Synpcc7942_1307 at 1334.866 kb on - strand, within Synpcc7942_1307 at 1334.866 kb on - strand, within Synpcc7942_1307 at 1334.889 kb on + strand, within Synpcc7942_1307 at 1334.889 kb on - strand, within Synpcc7942_1307 at 1334.891 kb on + strand, within Synpcc7942_1307 at 1334.923 kb on - strand at 1334.961 kb on - strand at 1334.965 kb on + strand at 1334.965 kb on - strand at 1334.966 kb on - strand at 1334.968 kb on - strand at 1334.983 kb on - strand at 1335.007 kb on - strand at 1335.009 kb on + strand at 1335.009 kb on + strand at 1335.022 kb on + strand at 1335.048 kb on + strand at 1335.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,333,201 + Synpcc7942_1305 0.85 +0.9 1,333,219 + Synpcc7942_1305 0.86 -2.9 1,333,222 - Synpcc7942_1305 0.86 -0.2 1,333,222 - Synpcc7942_1305 0.86 -3.6 1,333,222 - Synpcc7942_1305 0.86 -1.1 1,333,231 + Synpcc7942_1305 0.87 -1.5 1,333,239 - Synpcc7942_1305 0.87 -1.6 1,333,253 - Synpcc7942_1305 0.88 -2.7 1,333,301 + -1.1 1,333,303 - -2.0 1,333,311 - -2.6 1,333,340 + +0.2 1,333,348 - -2.6 1,333,357 + -2.6 1,333,376 - -1.7 1,333,401 - -2.2 1,333,431 - -3.9 1,333,509 - -1.1 1,334,096 + +2.1 1,334,120 + -1.7 1,334,185 - +0.6 1,334,235 + +4.6 1,334,261 - +0.6 1,334,283 - Synpcc7942_1307 0.12 -0.2 1,334,283 - Synpcc7942_1307 0.12 +2.0 1,334,283 - Synpcc7942_1307 0.12 +2.0 1,334,283 - Synpcc7942_1307 0.12 +3.4 1,334,295 - Synpcc7942_1307 0.14 -1.9 1,334,310 + Synpcc7942_1307 0.16 +2.5 1,334,310 + Synpcc7942_1307 0.16 +1.3 1,334,310 + Synpcc7942_1307 0.16 +1.9 1,334,313 - Synpcc7942_1307 0.16 -0.9 1,334,318 - Synpcc7942_1307 0.17 -1.0 1,334,318 - Synpcc7942_1307 0.17 -0.7 1,334,327 + Synpcc7942_1307 0.18 -1.4 1,334,352 + Synpcc7942_1307 0.21 +2.3 1,334,352 + Synpcc7942_1307 0.21 +3.4 1,334,352 + Synpcc7942_1307 0.21 -0.2 1,334,352 + Synpcc7942_1307 0.21 +2.6 1,334,352 + Synpcc7942_1307 0.21 -3.0 1,334,354 + Synpcc7942_1307 0.21 +0.7 1,334,360 - Synpcc7942_1307 0.22 -1.5 1,334,360 - Synpcc7942_1307 0.22 -1.1 1,334,377 + Synpcc7942_1307 0.24 +1.4 1,334,385 - Synpcc7942_1307 0.25 +1.1 1,334,394 + Synpcc7942_1307 0.26 -0.2 1,334,441 + Synpcc7942_1307 0.32 -1.2 1,334,459 - Synpcc7942_1307 0.34 +2.5 1,334,466 - +0.5 1,334,478 - Synpcc7942_1307 0.36 +2.7 1,334,514 + Synpcc7942_1307 0.41 +2.5 1,334,523 + Synpcc7942_1307 0.42 -2.5 1,334,536 - Synpcc7942_1307 0.44 +0.8 1,334,536 - +2.7 1,334,604 - Synpcc7942_1307 0.52 +1.7 1,334,645 - Synpcc7942_1307 0.57 -0.7 1,334,655 + Synpcc7942_1307 0.58 +3.9 1,334,726 + Synpcc7942_1307 0.67 -0.6 1,334,727 + Synpcc7942_1307 0.67 -0.9 1,334,727 + Synpcc7942_1307 0.67 +1.9 1,334,762 + Synpcc7942_1307 0.71 -1.1 1,334,778 + Synpcc7942_1307 0.73 +2.8 1,334,783 + Synpcc7942_1307 0.74 +0.6 1,334,800 + Synpcc7942_1307 0.76 +0.8 1,334,853 + Synpcc7942_1307 0.82 +1.1 1,334,861 - Synpcc7942_1307 0.83 +1.4 1,334,861 - Synpcc7942_1307 0.83 +2.0 1,334,866 - Synpcc7942_1307 0.84 +4.9 1,334,866 - Synpcc7942_1307 0.84 -1.5 1,334,889 + Synpcc7942_1307 0.87 +0.1 1,334,889 - Synpcc7942_1307 0.87 -1.4 1,334,891 + Synpcc7942_1307 0.87 +0.4 1,334,923 - -1.1 1,334,961 - -0.1 1,334,965 + +2.1 1,334,965 - -1.5 1,334,966 - +0.8 1,334,968 - +0.9 1,334,983 - -2.2 1,335,007 - +0.7 1,335,009 + -2.5 1,335,009 + -0.6 1,335,022 + -3.0 1,335,048 + -2.2 1,335,050 - -1.5
Or see this region's nucleotide sequence