Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lap and gmk are separated by 168 nucleotides gmk and Synpcc7942_1192 are separated by 89 nucleotides Synpcc7942_1192 and Synpcc7942_1193 are separated by 92 nucleotides
Synpcc7942_1190: lap - leucyl aminopeptidase, at 1,218,720 to 1,220,180
lap
Synpcc7942_1191: gmk - guanylate kinase, at 1,220,349 to 1,220,900
gmk
Synpcc7942_1192: Synpcc7942_1192 - hypothetical protein, at 1,220,990 to 1,221,583
_1192
Synpcc7942_1193: Synpcc7942_1193 - Phospholipid/glycerol acyltransferase, at 1,221,676 to 1,222,398
_1193
Position (kb)
1220
1221
1222 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 1220.019 kb on - strand, within lap at 1220.021 kb on + strand, within lap at 1220.068 kb on + strand at 1220.098 kb on - strand at 1220.112 kb on + strand at 1220.113 kb on - strand at 1220.120 kb on - strand at 1220.123 kb on + strand at 1220.124 kb on + strand at 1220.131 kb on - strand at 1220.131 kb on - strand at 1220.137 kb on - strand at 1220.140 kb on + strand at 1220.143 kb on - strand at 1220.144 kb on + strand at 1220.145 kb on + strand at 1220.154 kb on - strand at 1220.154 kb on - strand at 1220.154 kb on - strand at 1220.214 kb on - strand at 1220.218 kb on + strand at 1220.220 kb on + strand at 1220.225 kb on + strand at 1220.233 kb on - strand at 1220.248 kb on + strand at 1220.248 kb on + strand at 1220.254 kb on - strand at 1220.265 kb on + strand at 1220.270 kb on + strand at 1220.278 kb on - strand at 1220.278 kb on - strand at 1220.282 kb on + strand at 1220.286 kb on + strand at 1220.297 kb on - strand at 1220.308 kb on + strand at 1220.316 kb on - strand at 1220.316 kb on - strand at 1220.933 kb on + strand at 1220.940 kb on - strand at 1220.962 kb on - strand at 1221.020 kb on + strand at 1221.059 kb on - strand, within Synpcc7942_1192 at 1221.061 kb on + strand, within Synpcc7942_1192 at 1221.102 kb on + strand, within Synpcc7942_1192 at 1221.122 kb on - strand, within Synpcc7942_1192 at 1221.122 kb on - strand, within Synpcc7942_1192 at 1221.157 kb on + strand, within Synpcc7942_1192 at 1221.165 kb on - strand, within Synpcc7942_1192 at 1221.202 kb on + strand, within Synpcc7942_1192 at 1221.215 kb on - strand, within Synpcc7942_1192 at 1221.232 kb on - strand, within Synpcc7942_1192 at 1221.245 kb on + strand, within Synpcc7942_1192 at 1221.245 kb on + strand, within Synpcc7942_1192 at 1221.250 kb on + strand, within Synpcc7942_1192 at 1221.253 kb on - strand, within Synpcc7942_1192 at 1221.253 kb on - strand, within Synpcc7942_1192 at 1221.253 kb on - strand, within Synpcc7942_1192 at 1221.253 kb on - strand, within Synpcc7942_1192 at 1221.253 kb on - strand, within Synpcc7942_1192 at 1221.317 kb on + strand, within Synpcc7942_1192 at 1221.317 kb on + strand, within Synpcc7942_1192 at 1221.325 kb on - strand, within Synpcc7942_1192 at 1221.390 kb on + strand, within Synpcc7942_1192 at 1221.403 kb on - strand, within Synpcc7942_1192 at 1221.495 kb on + strand, within Synpcc7942_1192 at 1221.498 kb on + strand, within Synpcc7942_1192 at 1221.512 kb on - strand, within Synpcc7942_1192 at 1221.551 kb on - strand at 1221.626 kb on - strand at 1221.673 kb on + strand at 1221.673 kb on + strand at 1221.673 kb on + strand at 1221.674 kb on + strand at 1221.681 kb on - strand at 1221.681 kb on - strand at 1221.681 kb on - strand at 1221.681 kb on - strand at 1221.682 kb on - strand at 1221.736 kb on + strand at 1221.750 kb on + strand, within Synpcc7942_1193 at 1221.763 kb on - strand, within Synpcc7942_1193 at 1222.417 kb on - strand at 1222.420 kb on + strand at 1222.420 kb on + strand at 1222.427 kb on - strand at 1222.440 kb on + strand at 1222.440 kb on + strand at 1222.448 kb on - strand at 1222.448 kb on - strand at 1222.451 kb on + strand at 1222.467 kb on + strand at 1222.467 kb on - strand at 1222.485 kb on + strand at 1222.485 kb on + strand at 1222.537 kb on + strand at 1222.542 kb on + strand at 1222.542 kb on + strand at 1222.545 kb on - strand at 1222.545 kb on - strand at 1222.551 kb on - strand at 1222.551 kb on - strand at 1222.577 kb on + strand at 1222.577 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,220,019 - lap Synpcc7942_1190 0.89 -2.7 1,220,021 + lap Synpcc7942_1190 0.89 -2.3 1,220,068 + -3.4 1,220,098 - -2.1 1,220,112 + -5.1 1,220,113 - -1.6 1,220,120 - -4.0 1,220,123 + -3.1 1,220,124 + -1.7 1,220,131 - -0.2 1,220,131 - -0.2 1,220,137 - -4.1 1,220,140 + -1.7 1,220,143 - -3.0 1,220,144 + -3.3 1,220,145 + -0.9 1,220,154 - -2.3 1,220,154 - -3.8 1,220,154 - -0.1 1,220,214 - -2.1 1,220,218 + -1.7 1,220,220 + -2.7 1,220,225 + +3.9 1,220,233 - -0.9 1,220,248 + +0.1 1,220,248 + -1.3 1,220,254 - +3.5 1,220,265 + +2.9 1,220,270 + +0.8 1,220,278 - -2.7 1,220,278 - -2.1 1,220,282 + -1.3 1,220,286 + +1.0 1,220,297 - +2.0 1,220,308 + -0.1 1,220,316 - +0.6 1,220,316 - +0.4 1,220,933 + -1.5 1,220,940 - +2.8 1,220,962 - +1.2 1,221,020 + -1.4 1,221,059 - Synpcc7942_1192 0.12 -4.1 1,221,061 + Synpcc7942_1192 0.12 -0.2 1,221,102 + Synpcc7942_1192 0.19 -1.4 1,221,122 - Synpcc7942_1192 0.22 -1.5 1,221,122 - Synpcc7942_1192 0.22 -3.0 1,221,157 + Synpcc7942_1192 0.28 -3.5 1,221,165 - Synpcc7942_1192 0.29 -2.1 1,221,202 + Synpcc7942_1192 0.36 -3.8 1,221,215 - Synpcc7942_1192 0.38 -3.4 1,221,232 - Synpcc7942_1192 0.41 -1.8 1,221,245 + Synpcc7942_1192 0.43 -2.0 1,221,245 + Synpcc7942_1192 0.43 -0.7 1,221,250 + Synpcc7942_1192 0.44 -3.4 1,221,253 - Synpcc7942_1192 0.44 +0.1 1,221,253 - Synpcc7942_1192 0.44 -1.8 1,221,253 - Synpcc7942_1192 0.44 -4.6 1,221,253 - Synpcc7942_1192 0.44 -1.2 1,221,253 - Synpcc7942_1192 0.44 -0.7 1,221,317 + Synpcc7942_1192 0.55 -3.0 1,221,317 + Synpcc7942_1192 0.55 -1.2 1,221,325 - Synpcc7942_1192 0.56 -0.5 1,221,390 + Synpcc7942_1192 0.67 -2.5 1,221,403 - Synpcc7942_1192 0.70 -2.1 1,221,495 + Synpcc7942_1192 0.85 -2.4 1,221,498 + Synpcc7942_1192 0.86 +0.1 1,221,512 - Synpcc7942_1192 0.88 -1.4 1,221,551 - -3.1 1,221,626 - -3.5 1,221,673 + -2.1 1,221,673 + -1.3 1,221,673 + +1.1 1,221,674 + -0.3 1,221,681 - -1.6 1,221,681 - -1.2 1,221,681 - -2.1 1,221,681 - -1.1 1,221,682 - -0.0 1,221,736 + -0.4 1,221,750 + Synpcc7942_1193 0.10 -1.9 1,221,763 - Synpcc7942_1193 0.12 -2.7 1,222,417 - +0.3 1,222,420 + +4.9 1,222,420 + +5.4 1,222,427 - -1.8 1,222,440 + -4.5 1,222,440 + -2.5 1,222,448 - -4.4 1,222,448 - -0.9 1,222,451 + -2.4 1,222,467 + -1.4 1,222,467 - -1.1 1,222,485 + +1.9 1,222,485 + +1.3 1,222,537 + -2.0 1,222,542 + -1.7 1,222,542 + +2.8 1,222,545 - -3.0 1,222,545 - +0.9 1,222,551 - -0.7 1,222,551 - +0.6 1,222,577 + -1.4 1,222,577 + -0.2
Or see this region's nucleotide sequence