Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ndhK and ndhJ overlap by 8 nucleotides ndhJ and Synpcc7942_1183 are separated by 166 nucleotides Synpcc7942_1183 and Synpcc7942_1184 are separated by 24 nucleotides
Synpcc7942_1181: ndhK - NADH dehydrogenase subunit B, at 1,212,095 to 1,212,802
ndhK
Synpcc7942_1182: ndhJ - NADH dehydrogenase subunit J, at 1,212,795 to 1,213,313
ndhJ
Synpcc7942_1183: Synpcc7942_1183 - hypothetical protein, at 1,213,480 to 1,214,214
_1183
Synpcc7942_1184: Synpcc7942_1184 - 6-pyruvoyl-tetrahydropterin synthase-like protein, at 1,214,239 to 1,215,141
_1184
Position (kb)
1213
1214
1215 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1212.733 kb on - strand at 1212.745 kb on - strand at 1213.349 kb on - strand at 1213.359 kb on - strand at 1213.363 kb on + strand at 1213.364 kb on + strand at 1213.387 kb on - strand at 1213.431 kb on + strand at 1213.449 kb on - strand at 1213.450 kb on - strand at 1213.467 kb on + strand at 1213.488 kb on - strand at 1213.491 kb on + strand at 1213.506 kb on - strand at 1213.524 kb on + strand at 1213.572 kb on + strand, within Synpcc7942_1183 at 1213.580 kb on - strand, within Synpcc7942_1183 at 1213.625 kb on + strand, within Synpcc7942_1183 at 1213.637 kb on + strand, within Synpcc7942_1183 at 1213.637 kb on + strand, within Synpcc7942_1183 at 1213.656 kb on + strand, within Synpcc7942_1183 at 1213.656 kb on + strand, within Synpcc7942_1183 at 1213.657 kb on - strand, within Synpcc7942_1183 at 1213.661 kb on + strand, within Synpcc7942_1183 at 1213.664 kb on - strand, within Synpcc7942_1183 at 1213.770 kb on - strand, within Synpcc7942_1183 at 1213.777 kb on - strand, within Synpcc7942_1183 at 1213.784 kb on + strand, within Synpcc7942_1183 at 1213.810 kb on + strand, within Synpcc7942_1183 at 1213.847 kb on + strand, within Synpcc7942_1183 at 1213.860 kb on - strand, within Synpcc7942_1183 at 1213.873 kb on + strand, within Synpcc7942_1183 at 1213.881 kb on - strand, within Synpcc7942_1183 at 1213.881 kb on - strand, within Synpcc7942_1183 at 1213.886 kb on + strand, within Synpcc7942_1183 at 1213.971 kb on + strand, within Synpcc7942_1183 at 1213.979 kb on - strand, within Synpcc7942_1183 at 1213.999 kb on + strand, within Synpcc7942_1183 at 1214.022 kb on + strand, within Synpcc7942_1183 at 1214.048 kb on - strand, within Synpcc7942_1183 at 1214.104 kb on - strand, within Synpcc7942_1183 at 1214.154 kb on + strand at 1214.154 kb on + strand at 1214.162 kb on - strand at 1214.169 kb on + strand at 1214.177 kb on - strand at 1214.209 kb on + strand at 1214.228 kb on + strand at 1214.235 kb on - strand at 1214.260 kb on - strand at 1214.275 kb on + strand at 1214.275 kb on + strand at 1214.275 kb on + strand at 1214.283 kb on - strand at 1214.330 kb on - strand, within Synpcc7942_1184 at 1214.411 kb on - strand, within Synpcc7942_1184 at 1214.435 kb on + strand, within Synpcc7942_1184 at 1214.649 kb on + strand, within Synpcc7942_1184 at 1214.663 kb on + strand, within Synpcc7942_1184 at 1214.671 kb on - strand, within Synpcc7942_1184 at 1214.704 kb on + strand, within Synpcc7942_1184 at 1214.706 kb on + strand, within Synpcc7942_1184 at 1214.706 kb on + strand, within Synpcc7942_1184 at 1214.728 kb on + strand, within Synpcc7942_1184 at 1214.804 kb on + strand, within Synpcc7942_1184 at 1214.819 kb on - strand, within Synpcc7942_1184 at 1214.834 kb on + strand, within Synpcc7942_1184 at 1214.865 kb on + strand, within Synpcc7942_1184 at 1214.877 kb on + strand, within Synpcc7942_1184 at 1214.877 kb on + strand, within Synpcc7942_1184 at 1214.887 kb on + strand, within Synpcc7942_1184 at 1214.895 kb on - strand, within Synpcc7942_1184 at 1214.895 kb on - strand, within Synpcc7942_1184 at 1214.934 kb on + strand, within Synpcc7942_1184 at 1214.946 kb on - strand, within Synpcc7942_1184 at 1214.968 kb on + strand, within Synpcc7942_1184 at 1215.044 kb on - strand, within Synpcc7942_1184 at 1215.044 kb on - strand, within Synpcc7942_1184 at 1215.047 kb on + strand, within Synpcc7942_1184 at 1215.086 kb on + strand at 1215.086 kb on + strand at 1215.086 kb on + strand at 1215.086 kb on + strand at 1215.086 kb on + strand at 1215.088 kb on + strand at 1215.088 kb on + strand at 1215.088 kb on + strand at 1215.094 kb on - strand at 1215.094 kb on - strand at 1215.094 kb on - strand at 1215.094 kb on - strand at 1215.100 kb on + strand at 1215.100 kb on + strand at 1215.101 kb on - strand at 1215.113 kb on - strand at 1215.115 kb on + strand at 1215.135 kb on + strand at 1215.147 kb on + strand at 1215.182 kb on + strand at 1215.192 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,212,733 - +0.4 1,212,745 - -1.5 1,213,349 - +0.3 1,213,359 - -1.5 1,213,363 + -0.9 1,213,364 + -0.2 1,213,387 - -1.5 1,213,431 + +0.4 1,213,449 - -1.9 1,213,450 - -1.7 1,213,467 + -2.7 1,213,488 - -1.1 1,213,491 + -0.1 1,213,506 - -2.9 1,213,524 + +0.5 1,213,572 + Synpcc7942_1183 0.13 -0.7 1,213,580 - Synpcc7942_1183 0.14 -2.5 1,213,625 + Synpcc7942_1183 0.20 -2.9 1,213,637 + Synpcc7942_1183 0.21 -1.2 1,213,637 + Synpcc7942_1183 0.21 -1.6 1,213,656 + Synpcc7942_1183 0.24 -3.1 1,213,656 + Synpcc7942_1183 0.24 -3.5 1,213,657 - Synpcc7942_1183 0.24 -0.7 1,213,661 + Synpcc7942_1183 0.25 -2.1 1,213,664 - Synpcc7942_1183 0.25 -2.1 1,213,770 - Synpcc7942_1183 0.39 -2.4 1,213,777 - Synpcc7942_1183 0.40 -1.4 1,213,784 + Synpcc7942_1183 0.41 -2.4 1,213,810 + Synpcc7942_1183 0.45 +0.1 1,213,847 + Synpcc7942_1183 0.50 -1.6 1,213,860 - Synpcc7942_1183 0.52 -1.1 1,213,873 + Synpcc7942_1183 0.53 -3.2 1,213,881 - Synpcc7942_1183 0.55 -2.9 1,213,881 - Synpcc7942_1183 0.55 -3.2 1,213,886 + Synpcc7942_1183 0.55 -1.5 1,213,971 + Synpcc7942_1183 0.67 +0.3 1,213,979 - Synpcc7942_1183 0.68 +0.1 1,213,999 + Synpcc7942_1183 0.71 -2.3 1,214,022 + Synpcc7942_1183 0.74 -0.7 1,214,048 - Synpcc7942_1183 0.77 -2.7 1,214,104 - Synpcc7942_1183 0.85 -3.6 1,214,154 + -1.1 1,214,154 + -1.8 1,214,162 - -1.7 1,214,169 + +1.4 1,214,177 - -0.8 1,214,209 + +0.5 1,214,228 + +1.0 1,214,235 - +0.0 1,214,260 - -0.9 1,214,275 + -1.2 1,214,275 + +0.9 1,214,275 + -3.6 1,214,283 - -1.1 1,214,330 - Synpcc7942_1184 0.10 -0.6 1,214,411 - Synpcc7942_1184 0.19 -3.5 1,214,435 + Synpcc7942_1184 0.22 -1.3 1,214,649 + Synpcc7942_1184 0.45 -2.9 1,214,663 + Synpcc7942_1184 0.47 -1.9 1,214,671 - Synpcc7942_1184 0.48 -2.8 1,214,704 + Synpcc7942_1184 0.51 -0.9 1,214,706 + Synpcc7942_1184 0.52 +0.1 1,214,706 + Synpcc7942_1184 0.52 -1.0 1,214,728 + Synpcc7942_1184 0.54 -2.9 1,214,804 + Synpcc7942_1184 0.63 -2.7 1,214,819 - Synpcc7942_1184 0.64 -0.4 1,214,834 + Synpcc7942_1184 0.66 -2.9 1,214,865 + Synpcc7942_1184 0.69 -0.4 1,214,877 + Synpcc7942_1184 0.71 +0.7 1,214,877 + Synpcc7942_1184 0.71 -2.7 1,214,887 + Synpcc7942_1184 0.72 -0.5 1,214,895 - Synpcc7942_1184 0.73 -0.7 1,214,895 - Synpcc7942_1184 0.73 -2.8 1,214,934 + Synpcc7942_1184 0.77 -1.6 1,214,946 - Synpcc7942_1184 0.78 -0.7 1,214,968 + Synpcc7942_1184 0.81 -3.2 1,215,044 - Synpcc7942_1184 0.89 -2.7 1,215,044 - Synpcc7942_1184 0.89 -1.4 1,215,047 + Synpcc7942_1184 0.89 -1.5 1,215,086 + -1.9 1,215,086 + +0.1 1,215,086 + -1.1 1,215,086 + +0.7 1,215,086 + +1.0 1,215,088 + -0.3 1,215,088 + -1.5 1,215,088 + +3.5 1,215,094 - -0.7 1,215,094 - -1.3 1,215,094 - -3.5 1,215,094 - -0.5 1,215,100 + -0.4 1,215,100 + -0.2 1,215,101 - -0.5 1,215,113 - -0.9 1,215,115 + +1.4 1,215,135 + -3.0 1,215,147 + +0.8 1,215,182 + -1.3 1,215,192 - -1.4
Or see this region's nucleotide sequence