Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rnb and Synpcc7942_1125 are separated by 96 nucleotides Synpcc7942_1125 and Synpcc7942_1126 are separated by 57 nucleotides
Synpcc7942_1124: rnb - Exoribonuclease II, at 1,141,288 to 1,143,300
rnb
Synpcc7942_1125: Synpcc7942_1125 - putative CheA signal transduction histidine kinase, no good domain identification, at 1,143,397 to 1,144,332
_1125
Synpcc7942_1126: Synpcc7942_1126 - ABC transporter permease protein, at 1,144,390 to 1,145,223
_1126
Position (kb)
1143
1144
1145 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1143.263 kb on + strand at 1143.263 kb on + strand at 1143.282 kb on + strand at 1143.288 kb on - strand at 1143.288 kb on - strand at 1143.288 kb on - strand at 1143.288 kb on - strand at 1143.289 kb on - strand at 1143.294 kb on + strand at 1143.303 kb on - strand at 1143.333 kb on - strand at 1143.335 kb on + strand at 1143.335 kb on + strand at 1143.349 kb on - strand at 1143.359 kb on + strand at 1143.366 kb on + strand at 1143.369 kb on - strand at 1143.369 kb on - strand at 1143.379 kb on - strand at 1143.385 kb on + strand at 1143.386 kb on + strand at 1143.463 kb on + strand at 1143.463 kb on + strand at 1143.471 kb on - strand at 1143.472 kb on + strand at 1143.480 kb on - strand at 1143.542 kb on + strand, within Synpcc7942_1125 at 1143.542 kb on + strand, within Synpcc7942_1125 at 1143.544 kb on + strand, within Synpcc7942_1125 at 1143.544 kb on + strand, within Synpcc7942_1125 at 1143.544 kb on + strand, within Synpcc7942_1125 at 1143.544 kb on + strand, within Synpcc7942_1125 at 1143.550 kb on - strand, within Synpcc7942_1125 at 1143.550 kb on - strand, within Synpcc7942_1125 at 1143.550 kb on - strand, within Synpcc7942_1125 at 1143.552 kb on - strand, within Synpcc7942_1125 at 1143.552 kb on - strand, within Synpcc7942_1125 at 1143.552 kb on - strand, within Synpcc7942_1125 at 1143.560 kb on - strand, within Synpcc7942_1125 at 1143.564 kb on - strand, within Synpcc7942_1125 at 1143.568 kb on + strand, within Synpcc7942_1125 at 1143.611 kb on + strand, within Synpcc7942_1125 at 1143.611 kb on + strand, within Synpcc7942_1125 at 1143.611 kb on + strand, within Synpcc7942_1125 at 1143.611 kb on + strand, within Synpcc7942_1125 at 1143.611 kb on + strand, within Synpcc7942_1125 at 1143.619 kb on - strand at 1143.626 kb on - strand, within Synpcc7942_1125 at 1143.641 kb on - strand, within Synpcc7942_1125 at 1143.642 kb on + strand, within Synpcc7942_1125 at 1143.658 kb on + strand, within Synpcc7942_1125 at 1143.724 kb on - strand, within Synpcc7942_1125 at 1143.726 kb on + strand, within Synpcc7942_1125 at 1143.727 kb on - strand, within Synpcc7942_1125 at 1143.766 kb on + strand, within Synpcc7942_1125 at 1143.792 kb on - strand, within Synpcc7942_1125 at 1143.808 kb on + strand, within Synpcc7942_1125 at 1143.808 kb on + strand, within Synpcc7942_1125 at 1143.829 kb on - strand, within Synpcc7942_1125 at 1143.829 kb on - strand, within Synpcc7942_1125 at 1143.859 kb on - strand, within Synpcc7942_1125 at 1143.936 kb on + strand, within Synpcc7942_1125 at 1143.956 kb on - strand, within Synpcc7942_1125 at 1143.986 kb on + strand, within Synpcc7942_1125 at 1143.994 kb on + strand, within Synpcc7942_1125 at 1144.004 kb on - strand, within Synpcc7942_1125 at 1144.015 kb on - strand, within Synpcc7942_1125 at 1144.016 kb on + strand, within Synpcc7942_1125 at 1144.043 kb on + strand, within Synpcc7942_1125 at 1144.051 kb on - strand, within Synpcc7942_1125 at 1144.106 kb on + strand, within Synpcc7942_1125 at 1144.114 kb on - strand, within Synpcc7942_1125 at 1144.119 kb on - strand, within Synpcc7942_1125 at 1144.171 kb on - strand, within Synpcc7942_1125 at 1144.176 kb on - strand, within Synpcc7942_1125 at 1144.176 kb on - strand, within Synpcc7942_1125 at 1144.185 kb on - strand, within Synpcc7942_1125 at 1144.198 kb on + strand, within Synpcc7942_1125 at 1144.254 kb on + strand at 1144.254 kb on + strand at 1144.257 kb on - strand at 1144.284 kb on + strand at 1144.286 kb on + strand at 1144.286 kb on + strand at 1144.290 kb on - strand at 1144.308 kb on + strand at 1144.308 kb on + strand at 1144.311 kb on - strand at 1144.316 kb on - strand at 1144.327 kb on + strand at 1144.327 kb on + strand at 1144.328 kb on + strand at 1144.335 kb on - strand at 1144.335 kb on - strand at 1144.366 kb on + strand at 1144.374 kb on - strand at 1144.374 kb on - strand at 1144.390 kb on + strand at 1144.512 kb on + strand, within Synpcc7942_1126 at 1144.528 kb on - strand, within Synpcc7942_1126 at 1144.585 kb on + strand, within Synpcc7942_1126 at 1144.611 kb on + strand, within Synpcc7942_1126 at 1144.619 kb on - strand, within Synpcc7942_1126 at 1144.891 kb on - strand, within Synpcc7942_1126 at 1144.904 kb on - strand, within Synpcc7942_1126 at 1144.940 kb on + strand, within Synpcc7942_1126 at 1144.985 kb on - strand, within Synpcc7942_1126 at 1145.002 kb on + strand, within Synpcc7942_1126 at 1145.051 kb on + strand, within Synpcc7942_1126 at 1145.051 kb on + strand, within Synpcc7942_1126 at 1145.082 kb on - strand, within Synpcc7942_1126 at 1145.220 kb on + strand at 1145.242 kb on + strand at 1145.274 kb on + strand at 1145.274 kb on + strand at 1145.282 kb on - strand at 1145.287 kb on - strand at 1145.313 kb on + strand at 1145.313 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,143,263 + -1.6 1,143,263 + -3.8 1,143,282 + -1.9 1,143,288 - -2.3 1,143,288 - -1.9 1,143,288 - -3.0 1,143,288 - -4.6 1,143,289 - -3.2 1,143,294 + +0.1 1,143,303 - -2.8 1,143,333 - +0.3 1,143,335 + +0.5 1,143,335 + -0.2 1,143,349 - +1.3 1,143,359 + +2.0 1,143,366 + -0.5 1,143,369 - -1.6 1,143,369 - -2.6 1,143,379 - -2.1 1,143,385 + -0.5 1,143,386 + -0.4 1,143,463 + -0.1 1,143,463 + -2.4 1,143,471 - -2.7 1,143,472 + -1.5 1,143,480 - +1.9 1,143,542 + Synpcc7942_1125 0.15 +1.2 1,143,542 + Synpcc7942_1125 0.15 -2.2 1,143,544 + Synpcc7942_1125 0.16 -2.4 1,143,544 + Synpcc7942_1125 0.16 -1.2 1,143,544 + Synpcc7942_1125 0.16 -0.4 1,143,544 + Synpcc7942_1125 0.16 -1.5 1,143,550 - Synpcc7942_1125 0.16 +0.4 1,143,550 - Synpcc7942_1125 0.16 -2.1 1,143,550 - Synpcc7942_1125 0.16 -1.6 1,143,552 - Synpcc7942_1125 0.17 -3.4 1,143,552 - Synpcc7942_1125 0.17 +0.5 1,143,552 - Synpcc7942_1125 0.17 +0.3 1,143,560 - Synpcc7942_1125 0.17 -2.2 1,143,564 - Synpcc7942_1125 0.18 +1.3 1,143,568 + Synpcc7942_1125 0.18 -0.7 1,143,611 + Synpcc7942_1125 0.23 -0.2 1,143,611 + Synpcc7942_1125 0.23 -0.2 1,143,611 + Synpcc7942_1125 0.23 -1.9 1,143,611 + Synpcc7942_1125 0.23 -2.4 1,143,611 + Synpcc7942_1125 0.23 -1.3 1,143,619 - +0.1 1,143,626 - Synpcc7942_1125 0.24 +0.2 1,143,641 - Synpcc7942_1125 0.26 +0.2 1,143,642 + Synpcc7942_1125 0.26 -1.6 1,143,658 + Synpcc7942_1125 0.28 -1.0 1,143,724 - Synpcc7942_1125 0.35 +0.9 1,143,726 + Synpcc7942_1125 0.35 -1.2 1,143,727 - Synpcc7942_1125 0.35 +0.1 1,143,766 + Synpcc7942_1125 0.39 -2.1 1,143,792 - Synpcc7942_1125 0.42 -1.8 1,143,808 + Synpcc7942_1125 0.44 -2.2 1,143,808 + Synpcc7942_1125 0.44 -0.7 1,143,829 - Synpcc7942_1125 0.46 -1.3 1,143,829 - Synpcc7942_1125 0.46 +1.9 1,143,859 - Synpcc7942_1125 0.49 -0.2 1,143,936 + Synpcc7942_1125 0.58 -1.6 1,143,956 - Synpcc7942_1125 0.60 +1.7 1,143,986 + Synpcc7942_1125 0.63 -1.5 1,143,994 + Synpcc7942_1125 0.64 -1.3 1,144,004 - Synpcc7942_1125 0.65 -0.5 1,144,015 - Synpcc7942_1125 0.66 +0.1 1,144,016 + Synpcc7942_1125 0.66 -0.5 1,144,043 + Synpcc7942_1125 0.69 -0.9 1,144,051 - Synpcc7942_1125 0.70 -1.8 1,144,106 + Synpcc7942_1125 0.76 +3.2 1,144,114 - Synpcc7942_1125 0.77 +0.2 1,144,119 - Synpcc7942_1125 0.77 -3.1 1,144,171 - Synpcc7942_1125 0.83 +0.4 1,144,176 - Synpcc7942_1125 0.83 -0.9 1,144,176 - Synpcc7942_1125 0.83 +0.3 1,144,185 - Synpcc7942_1125 0.84 +1.9 1,144,198 + Synpcc7942_1125 0.86 +1.7 1,144,254 + +0.8 1,144,254 + +2.0 1,144,257 - -1.3 1,144,284 + -1.6 1,144,286 + +1.4 1,144,286 + -1.6 1,144,290 - -1.6 1,144,308 + -0.9 1,144,308 + -1.5 1,144,311 - +1.0 1,144,316 - +0.9 1,144,327 + +3.1 1,144,327 + +3.1 1,144,328 + +4.5 1,144,335 - -1.1 1,144,335 - -0.6 1,144,366 + -1.3 1,144,374 - -0.7 1,144,374 - -2.6 1,144,390 + -1.9 1,144,512 + Synpcc7942_1126 0.15 +1.9 1,144,528 - Synpcc7942_1126 0.17 -0.7 1,144,585 + Synpcc7942_1126 0.23 +0.2 1,144,611 + Synpcc7942_1126 0.26 +1.7 1,144,619 - Synpcc7942_1126 0.27 +0.3 1,144,891 - Synpcc7942_1126 0.60 +0.8 1,144,904 - Synpcc7942_1126 0.62 +1.1 1,144,940 + Synpcc7942_1126 0.66 -0.7 1,144,985 - Synpcc7942_1126 0.71 +2.1 1,145,002 + Synpcc7942_1126 0.73 +3.5 1,145,051 + Synpcc7942_1126 0.79 +2.1 1,145,051 + Synpcc7942_1126 0.79 +2.7 1,145,082 - Synpcc7942_1126 0.83 +1.1 1,145,220 + -0.4 1,145,242 + +3.0 1,145,274 + -3.2 1,145,274 + +1.4 1,145,282 - +2.2 1,145,287 - -1.1 1,145,313 + -0.7 1,145,313 + -2.8
Or see this region's nucleotide sequence