Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdhC and Synpcc7942_1069 are separated by 225 nucleotides Synpcc7942_1069 and Synpcc7942_1070 are separated by 2 nucleotides
Synpcc7942_1068: pdhC - branched-chain alpha-keto acid dehydrogenase subunit E2, at 1,079,478 to 1,080,773
pdhC
Synpcc7942_1069: Synpcc7942_1069 - hypothetical protein, at 1,080,999 to 1,081,727
_1069
Synpcc7942_1070: Synpcc7942_1070 - oxidoreductase aldo/keto reductase, at 1,081,730 to 1,082,851
_1070
Position (kb)
1080
1081
1082 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1080.806 kb on - strand at 1080.806 kb on - strand at 1080.880 kb on + strand at 1080.893 kb on - strand at 1080.893 kb on - strand at 1080.898 kb on + strand at 1080.908 kb on + strand at 1080.908 kb on + strand at 1080.908 kb on + strand at 1080.908 kb on + strand at 1080.908 kb on + strand at 1080.916 kb on - strand at 1080.923 kb on + strand at 1080.952 kb on - strand at 1080.953 kb on - strand at 1080.993 kb on - strand at 1080.998 kb on - strand at 1080.998 kb on - strand at 1081.029 kb on - strand at 1081.045 kb on + strand at 1081.053 kb on - strand at 1081.063 kb on - strand at 1081.072 kb on - strand, within Synpcc7942_1069 at 1081.080 kb on + strand, within Synpcc7942_1069 at 1081.088 kb on - strand, within Synpcc7942_1069 at 1081.102 kb on - strand, within Synpcc7942_1069 at 1081.109 kb on + strand, within Synpcc7942_1069 at 1081.109 kb on + strand, within Synpcc7942_1069 at 1081.117 kb on - strand, within Synpcc7942_1069 at 1081.117 kb on - strand, within Synpcc7942_1069 at 1081.117 kb on - strand, within Synpcc7942_1069 at 1081.118 kb on + strand, within Synpcc7942_1069 at 1081.182 kb on + strand, within Synpcc7942_1069 at 1081.187 kb on + strand, within Synpcc7942_1069 at 1081.195 kb on - strand, within Synpcc7942_1069 at 1081.210 kb on - strand, within Synpcc7942_1069 at 1081.212 kb on - strand, within Synpcc7942_1069 at 1081.244 kb on + strand, within Synpcc7942_1069 at 1081.244 kb on + strand, within Synpcc7942_1069 at 1081.248 kb on - strand, within Synpcc7942_1069 at 1081.252 kb on - strand, within Synpcc7942_1069 at 1081.262 kb on - strand, within Synpcc7942_1069 at 1081.267 kb on - strand, within Synpcc7942_1069 at 1081.267 kb on - strand, within Synpcc7942_1069 at 1081.272 kb on + strand, within Synpcc7942_1069 at 1081.280 kb on - strand, within Synpcc7942_1069 at 1081.287 kb on + strand, within Synpcc7942_1069 at 1081.288 kb on - strand, within Synpcc7942_1069 at 1081.291 kb on + strand, within Synpcc7942_1069 at 1081.298 kb on + strand, within Synpcc7942_1069 at 1081.305 kb on - strand, within Synpcc7942_1069 at 1081.307 kb on + strand, within Synpcc7942_1069 at 1081.307 kb on + strand, within Synpcc7942_1069 at 1081.330 kb on - strand, within Synpcc7942_1069 at 1081.377 kb on - strand, within Synpcc7942_1069 at 1081.377 kb on - strand, within Synpcc7942_1069 at 1081.399 kb on - strand, within Synpcc7942_1069 at 1081.409 kb on + strand, within Synpcc7942_1069 at 1081.409 kb on + strand, within Synpcc7942_1069 at 1081.409 kb on + strand, within Synpcc7942_1069 at 1081.409 kb on + strand, within Synpcc7942_1069 at 1081.413 kb on + strand, within Synpcc7942_1069 at 1081.417 kb on - strand, within Synpcc7942_1069 at 1081.424 kb on + strand, within Synpcc7942_1069 at 1081.424 kb on + strand, within Synpcc7942_1069 at 1081.432 kb on - strand, within Synpcc7942_1069 at 1081.435 kb on + strand, within Synpcc7942_1069 at 1081.472 kb on + strand, within Synpcc7942_1069 at 1081.480 kb on - strand, within Synpcc7942_1069 at 1081.504 kb on + strand, within Synpcc7942_1069 at 1081.521 kb on - strand, within Synpcc7942_1069 at 1081.521 kb on - strand, within Synpcc7942_1069 at 1081.526 kb on - strand, within Synpcc7942_1069 at 1081.532 kb on + strand, within Synpcc7942_1069 at 1081.558 kb on + strand, within Synpcc7942_1069 at 1081.559 kb on - strand, within Synpcc7942_1069 at 1081.566 kb on - strand, within Synpcc7942_1069 at 1081.578 kb on + strand, within Synpcc7942_1069 at 1081.578 kb on + strand, within Synpcc7942_1069 at 1081.578 kb on + strand, within Synpcc7942_1069 at 1081.578 kb on + strand, within Synpcc7942_1069 at 1081.585 kb on + strand, within Synpcc7942_1069 at 1081.586 kb on - strand, within Synpcc7942_1069 at 1081.586 kb on - strand, within Synpcc7942_1069 at 1081.594 kb on + strand, within Synpcc7942_1069 at 1081.610 kb on + strand, within Synpcc7942_1069 at 1081.618 kb on - strand, within Synpcc7942_1069 at 1081.635 kb on - strand, within Synpcc7942_1069 at 1081.635 kb on - strand, within Synpcc7942_1069 at 1081.644 kb on + strand, within Synpcc7942_1069 at 1081.644 kb on + strand, within Synpcc7942_1069 at 1081.652 kb on - strand, within Synpcc7942_1069 at 1081.652 kb on - strand, within Synpcc7942_1069 at 1081.700 kb on + strand at 1081.710 kb on - strand at 1081.718 kb on - strand at 1081.735 kb on - strand at 1081.735 kb on - strand at 1081.735 kb on - strand at 1081.738 kb on - strand at 1082.001 kb on + strand, within Synpcc7942_1070 at 1082.009 kb on - strand, within Synpcc7942_1070
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,080,806 - -1.5 1,080,806 - -2.6 1,080,880 + -0.4 1,080,893 - -0.5 1,080,893 - +0.5 1,080,898 + -0.0 1,080,908 + +0.4 1,080,908 + +0.6 1,080,908 + -1.4 1,080,908 + +1.4 1,080,908 + +1.9 1,080,916 - -0.8 1,080,923 + +0.6 1,080,952 - -0.7 1,080,953 - +0.8 1,080,993 - +1.9 1,080,998 - +0.5 1,080,998 - -0.6 1,081,029 - +1.1 1,081,045 + +3.0 1,081,053 - -1.9 1,081,063 - -1.0 1,081,072 - Synpcc7942_1069 0.10 -0.3 1,081,080 + Synpcc7942_1069 0.11 -2.4 1,081,088 - Synpcc7942_1069 0.12 +0.2 1,081,102 - Synpcc7942_1069 0.14 +0.1 1,081,109 + Synpcc7942_1069 0.15 -1.2 1,081,109 + Synpcc7942_1069 0.15 -0.3 1,081,117 - Synpcc7942_1069 0.16 -1.1 1,081,117 - Synpcc7942_1069 0.16 +1.1 1,081,117 - Synpcc7942_1069 0.16 -0.5 1,081,118 + Synpcc7942_1069 0.16 -0.9 1,081,182 + Synpcc7942_1069 0.25 +0.7 1,081,187 + Synpcc7942_1069 0.26 +0.4 1,081,195 - Synpcc7942_1069 0.27 -1.8 1,081,210 - Synpcc7942_1069 0.29 +1.8 1,081,212 - Synpcc7942_1069 0.29 -0.8 1,081,244 + Synpcc7942_1069 0.34 +0.7 1,081,244 + Synpcc7942_1069 0.34 +0.1 1,081,248 - Synpcc7942_1069 0.34 -0.7 1,081,252 - Synpcc7942_1069 0.35 +0.3 1,081,262 - Synpcc7942_1069 0.36 +1.2 1,081,267 - Synpcc7942_1069 0.37 -1.7 1,081,267 - Synpcc7942_1069 0.37 +0.6 1,081,272 + Synpcc7942_1069 0.37 -1.4 1,081,280 - Synpcc7942_1069 0.39 +0.4 1,081,287 + Synpcc7942_1069 0.40 +0.1 1,081,288 - Synpcc7942_1069 0.40 -2.0 1,081,291 + Synpcc7942_1069 0.40 -2.2 1,081,298 + Synpcc7942_1069 0.41 -1.9 1,081,305 - Synpcc7942_1069 0.42 +2.0 1,081,307 + Synpcc7942_1069 0.42 -1.8 1,081,307 + Synpcc7942_1069 0.42 -1.2 1,081,330 - Synpcc7942_1069 0.45 -0.9 1,081,377 - Synpcc7942_1069 0.52 -0.2 1,081,377 - Synpcc7942_1069 0.52 -0.5 1,081,399 - Synpcc7942_1069 0.55 -2.4 1,081,409 + Synpcc7942_1069 0.56 -0.3 1,081,409 + Synpcc7942_1069 0.56 -1.7 1,081,409 + Synpcc7942_1069 0.56 +1.2 1,081,409 + Synpcc7942_1069 0.56 -0.7 1,081,413 + Synpcc7942_1069 0.57 -1.2 1,081,417 - Synpcc7942_1069 0.57 -1.5 1,081,424 + Synpcc7942_1069 0.58 +0.2 1,081,424 + Synpcc7942_1069 0.58 -1.8 1,081,432 - Synpcc7942_1069 0.59 -1.7 1,081,435 + Synpcc7942_1069 0.60 -0.7 1,081,472 + Synpcc7942_1069 0.65 -2.1 1,081,480 - Synpcc7942_1069 0.66 -2.0 1,081,504 + Synpcc7942_1069 0.69 -1.6 1,081,521 - Synpcc7942_1069 0.72 +0.4 1,081,521 - Synpcc7942_1069 0.72 -0.4 1,081,526 - Synpcc7942_1069 0.72 -1.8 1,081,532 + Synpcc7942_1069 0.73 -2.9 1,081,558 + Synpcc7942_1069 0.77 +2.0 1,081,559 - Synpcc7942_1069 0.77 -2.3 1,081,566 - Synpcc7942_1069 0.78 -1.8 1,081,578 + Synpcc7942_1069 0.79 -0.6 1,081,578 + Synpcc7942_1069 0.79 +0.1 1,081,578 + Synpcc7942_1069 0.79 -0.9 1,081,578 + Synpcc7942_1069 0.79 -1.4 1,081,585 + Synpcc7942_1069 0.80 +0.5 1,081,586 - Synpcc7942_1069 0.81 +0.0 1,081,586 - Synpcc7942_1069 0.81 +0.9 1,081,594 + Synpcc7942_1069 0.82 -1.1 1,081,610 + Synpcc7942_1069 0.84 -3.0 1,081,618 - Synpcc7942_1069 0.85 -0.4 1,081,635 - Synpcc7942_1069 0.87 -0.1 1,081,635 - Synpcc7942_1069 0.87 -1.9 1,081,644 + Synpcc7942_1069 0.88 -1.4 1,081,644 + Synpcc7942_1069 0.88 -0.4 1,081,652 - Synpcc7942_1069 0.90 -1.6 1,081,652 - Synpcc7942_1069 0.90 -0.5 1,081,700 + +1.0 1,081,710 - +3.8 1,081,718 - -0.5 1,081,735 - +1.5 1,081,735 - +1.7 1,081,735 - +1.8 1,081,738 - -1.5 1,082,001 + Synpcc7942_1070 0.24 -0.9 1,082,009 - Synpcc7942_1070 0.25 -0.9
Or see this region's nucleotide sequence