Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpcE and cpcF are separated by 6 nucleotides cpcF and Synpcc7942_1056 are separated by 26 nucleotides Synpcc7942_1056 and thiE are separated by 38 nucleotides
Synpcc7942_1054: cpcE - PBS lyase HEAT-like repeat, at 1,065,228 to 1,066,049
cpcE
Synpcc7942_1055: cpcF - phycocyanin alpha-subunit phycocyanobilin lyase, at 1,066,056 to 1,066,679
cpcF
Synpcc7942_1056: Synpcc7942_1056 - transcriptional regulator, XRE family, at 1,066,706 to 1,067,488
_1056
Synpcc7942_1057: thiE - thiamine-phosphate pyrophosphorylase, at 1,067,527 to 1,068,558
thiE
Position (kb)
1066
1067
1068 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1066.053 kb on + strand at 1066.067 kb on + strand at 1066.073 kb on - strand at 1066.075 kb on - strand at 1066.300 kb on - strand, within cpcF at 1066.302 kb on - strand, within cpcF at 1066.406 kb on - strand, within cpcF at 1066.477 kb on + strand, within cpcF at 1066.682 kb on + strand at 1066.702 kb on - strand at 1066.720 kb on + strand at 1066.730 kb on + strand at 1066.733 kb on + strand at 1066.746 kb on - strand at 1066.757 kb on - strand at 1066.775 kb on + strand at 1066.805 kb on + strand, within Synpcc7942_1056 at 1066.805 kb on + strand, within Synpcc7942_1056 at 1066.805 kb on + strand, within Synpcc7942_1056 at 1066.813 kb on - strand, within Synpcc7942_1056 at 1066.828 kb on - strand, within Synpcc7942_1056 at 1066.829 kb on - strand, within Synpcc7942_1056 at 1066.830 kb on + strand, within Synpcc7942_1056 at 1066.838 kb on - strand, within Synpcc7942_1056 at 1066.856 kb on + strand, within Synpcc7942_1056 at 1066.856 kb on + strand, within Synpcc7942_1056 at 1066.863 kb on - strand, within Synpcc7942_1056 at 1066.875 kb on - strand, within Synpcc7942_1056 at 1066.888 kb on + strand, within Synpcc7942_1056 at 1066.898 kb on - strand, within Synpcc7942_1056 at 1066.910 kb on - strand, within Synpcc7942_1056 at 1066.962 kb on + strand, within Synpcc7942_1056 at 1066.972 kb on + strand, within Synpcc7942_1056 at 1066.988 kb on - strand, within Synpcc7942_1056 at 1067.032 kb on + strand, within Synpcc7942_1056 at 1067.032 kb on - strand, within Synpcc7942_1056 at 1067.051 kb on - strand, within Synpcc7942_1056 at 1067.058 kb on + strand, within Synpcc7942_1056 at 1067.071 kb on + strand, within Synpcc7942_1056 at 1067.103 kb on - strand, within Synpcc7942_1056 at 1067.111 kb on - strand, within Synpcc7942_1056 at 1067.130 kb on + strand, within Synpcc7942_1056 at 1067.138 kb on - strand, within Synpcc7942_1056 at 1067.151 kb on + strand, within Synpcc7942_1056 at 1067.170 kb on + strand, within Synpcc7942_1056 at 1067.185 kb on + strand, within Synpcc7942_1056 at 1067.193 kb on - strand, within Synpcc7942_1056 at 1067.221 kb on + strand, within Synpcc7942_1056 at 1067.227 kb on - strand, within Synpcc7942_1056 at 1067.262 kb on - strand, within Synpcc7942_1056 at 1067.278 kb on - strand, within Synpcc7942_1056 at 1067.295 kb on - strand, within Synpcc7942_1056 at 1067.300 kb on + strand, within Synpcc7942_1056 at 1067.316 kb on - strand, within Synpcc7942_1056 at 1067.379 kb on - strand, within Synpcc7942_1056 at 1067.390 kb on + strand, within Synpcc7942_1056 at 1067.416 kb on + strand at 1067.420 kb on - strand at 1067.454 kb on + strand at 1067.461 kb on + strand at 1067.466 kb on + strand at 1067.466 kb on + strand at 1067.466 kb on + strand at 1067.469 kb on - strand at 1067.469 kb on - strand at 1067.469 kb on - strand at 1067.474 kb on - strand at 1067.497 kb on + strand at 1067.497 kb on + strand at 1067.511 kb on + strand at 1067.511 kb on + strand at 1067.511 kb on + strand at 1067.519 kb on - strand at 1067.526 kb on + strand at 1067.539 kb on - strand at 1067.539 kb on - strand at 1067.562 kb on - strand at 1067.925 kb on - strand, within thiE
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,066,053 + +1.2 1,066,067 + +0.1 1,066,073 - +1.2 1,066,075 - +2.4 1,066,300 - cpcF Synpcc7942_1055 0.39 -0.5 1,066,302 - cpcF Synpcc7942_1055 0.39 -0.7 1,066,406 - cpcF Synpcc7942_1055 0.56 +1.1 1,066,477 + cpcF Synpcc7942_1055 0.67 -1.1 1,066,682 + -3.9 1,066,702 - -2.2 1,066,720 + -1.6 1,066,730 + -3.3 1,066,733 + +0.3 1,066,746 - -3.1 1,066,757 - -1.8 1,066,775 + -0.5 1,066,805 + Synpcc7942_1056 0.13 -2.6 1,066,805 + Synpcc7942_1056 0.13 -1.1 1,066,805 + Synpcc7942_1056 0.13 -1.9 1,066,813 - Synpcc7942_1056 0.14 -1.4 1,066,828 - Synpcc7942_1056 0.16 -4.0 1,066,829 - Synpcc7942_1056 0.16 -1.6 1,066,830 + Synpcc7942_1056 0.16 -4.4 1,066,838 - Synpcc7942_1056 0.17 -1.1 1,066,856 + Synpcc7942_1056 0.19 -0.2 1,066,856 + Synpcc7942_1056 0.19 -3.6 1,066,863 - Synpcc7942_1056 0.20 -2.2 1,066,875 - Synpcc7942_1056 0.22 -0.8 1,066,888 + Synpcc7942_1056 0.23 -1.5 1,066,898 - Synpcc7942_1056 0.25 -1.5 1,066,910 - Synpcc7942_1056 0.26 +0.6 1,066,962 + Synpcc7942_1056 0.33 -0.7 1,066,972 + Synpcc7942_1056 0.34 -2.1 1,066,988 - Synpcc7942_1056 0.36 -2.9 1,067,032 + Synpcc7942_1056 0.42 -0.3 1,067,032 - Synpcc7942_1056 0.42 -1.8 1,067,051 - Synpcc7942_1056 0.44 -1.4 1,067,058 + Synpcc7942_1056 0.45 -2.6 1,067,071 + Synpcc7942_1056 0.47 -3.9 1,067,103 - Synpcc7942_1056 0.51 -1.5 1,067,111 - Synpcc7942_1056 0.52 -2.9 1,067,130 + Synpcc7942_1056 0.54 -2.2 1,067,138 - Synpcc7942_1056 0.55 -0.1 1,067,151 + Synpcc7942_1056 0.57 -3.0 1,067,170 + Synpcc7942_1056 0.59 -2.9 1,067,185 + Synpcc7942_1056 0.61 -2.4 1,067,193 - Synpcc7942_1056 0.62 -2.1 1,067,221 + Synpcc7942_1056 0.66 -3.6 1,067,227 - Synpcc7942_1056 0.67 -2.6 1,067,262 - Synpcc7942_1056 0.71 -3.8 1,067,278 - Synpcc7942_1056 0.73 -2.8 1,067,295 - Synpcc7942_1056 0.75 -0.9 1,067,300 + Synpcc7942_1056 0.76 -2.8 1,067,316 - Synpcc7942_1056 0.78 -2.2 1,067,379 - Synpcc7942_1056 0.86 -4.5 1,067,390 + Synpcc7942_1056 0.87 -1.9 1,067,416 + -0.2 1,067,420 - -2.2 1,067,454 + -2.5 1,067,461 + -2.1 1,067,466 + -2.0 1,067,466 + -0.4 1,067,466 + -1.0 1,067,469 - -0.9 1,067,469 - -3.6 1,067,469 - +1.4 1,067,474 - -2.2 1,067,497 + -2.7 1,067,497 + -0.4 1,067,511 + -1.5 1,067,511 + -1.5 1,067,511 + -1.8 1,067,519 - +0.5 1,067,526 + +1.9 1,067,539 - +0.8 1,067,539 - -3.1 1,067,562 - +2.0 1,067,925 - thiE Synpcc7942_1057 0.39 -2.2
Or see this region's nucleotide sequence