Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpcB1 and cpcA1 are separated by 51 nucleotides cpcA1 and cpcH are separated by 105 nucleotides cpcH and cpcI are separated by 47 nucleotides
Synpcc7942_1047: cpcB1 - phycocyanin, beta subunit, at 1,060,318 to 1,060,839
cpcB1
Synpcc7942_1048: cpcA1 - phycocyanin, alpha subunit, at 1,060,891 to 1,061,382
cpcA1
Synpcc7942_1049: cpcH - phycobilisome rod linker polypeptide, at 1,061,488 to 1,062,309
cpcH
Synpcc7942_1050: cpcI - phycobilisome rod linker polypeptide, at 1,062,357 to 1,063,226
cpcI
Position (kb)
1061
1062
1063 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1061.473 kb on + strand at 1061.503 kb on - strand at 1061.622 kb on - strand, within cpcH at 1061.828 kb on + strand, within cpcH at 1061.838 kb on + strand, within cpcH at 1062.056 kb on + strand, within cpcH at 1062.096 kb on - strand, within cpcH at 1062.246 kb on + strand at 1062.293 kb on + strand at 1062.312 kb on - strand at 1062.321 kb on + strand at 1062.346 kb on - strand at 1062.406 kb on - strand at 1062.445 kb on + strand, within cpcI at 1062.471 kb on + strand, within cpcI at 1062.471 kb on + strand, within cpcI at 1062.584 kb on + strand, within cpcI at 1062.592 kb on - strand, within cpcI at 1062.592 kb on - strand, within cpcI at 1062.599 kb on + strand, within cpcI at 1062.599 kb on + strand, within cpcI at 1062.599 kb on + strand, within cpcI at 1062.607 kb on - strand, within cpcI at 1062.671 kb on - strand, within cpcI at 1062.686 kb on - strand, within cpcI at 1062.691 kb on - strand, within cpcI at 1062.737 kb on - strand, within cpcI at 1062.816 kb on + strand, within cpcI at 1062.821 kb on + strand, within cpcI at 1062.827 kb on - strand, within cpcI at 1062.839 kb on - strand, within cpcI at 1062.849 kb on - strand, within cpcI at 1063.012 kb on - strand, within cpcI at 1063.013 kb on - strand, within cpcI at 1063.019 kb on - strand, within cpcI at 1063.022 kb on + strand, within cpcI at 1063.228 kb on - strand at 1063.228 kb on - strand at 1063.228 kb on - strand at 1063.228 kb on - strand at 1063.228 kb on - strand at 1063.286 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,061,473 + -1.1 1,061,503 - -2.2 1,061,622 - cpcH Synpcc7942_1049 0.16 -2.0 1,061,828 + cpcH Synpcc7942_1049 0.41 +0.0 1,061,838 + cpcH Synpcc7942_1049 0.43 -1.0 1,062,056 + cpcH Synpcc7942_1049 0.69 -2.5 1,062,096 - cpcH Synpcc7942_1049 0.74 -1.3 1,062,246 + +2.0 1,062,293 + -0.4 1,062,312 - +0.8 1,062,321 + -0.5 1,062,346 - -1.5 1,062,406 - -1.1 1,062,445 + cpcI Synpcc7942_1050 0.10 +0.1 1,062,471 + cpcI Synpcc7942_1050 0.13 +1.5 1,062,471 + cpcI Synpcc7942_1050 0.13 -0.3 1,062,584 + cpcI Synpcc7942_1050 0.26 -1.5 1,062,592 - cpcI Synpcc7942_1050 0.27 -0.9 1,062,592 - cpcI Synpcc7942_1050 0.27 -1.3 1,062,599 + cpcI Synpcc7942_1050 0.28 +1.5 1,062,599 + cpcI Synpcc7942_1050 0.28 +0.5 1,062,599 + cpcI Synpcc7942_1050 0.28 +0.1 1,062,607 - cpcI Synpcc7942_1050 0.29 -3.4 1,062,671 - cpcI Synpcc7942_1050 0.36 -1.6 1,062,686 - cpcI Synpcc7942_1050 0.38 -2.7 1,062,691 - cpcI Synpcc7942_1050 0.38 -3.2 1,062,737 - cpcI Synpcc7942_1050 0.44 -1.5 1,062,816 + cpcI Synpcc7942_1050 0.53 -0.4 1,062,821 + cpcI Synpcc7942_1050 0.53 -0.9 1,062,827 - cpcI Synpcc7942_1050 0.54 +3.1 1,062,839 - cpcI Synpcc7942_1050 0.55 -0.6 1,062,849 - cpcI Synpcc7942_1050 0.57 -0.1 1,063,012 - cpcI Synpcc7942_1050 0.75 -2.6 1,063,013 - cpcI Synpcc7942_1050 0.75 +0.7 1,063,019 - cpcI Synpcc7942_1050 0.76 -1.0 1,063,022 + cpcI Synpcc7942_1050 0.76 -1.9 1,063,228 - -1.3 1,063,228 - -2.0 1,063,228 - +3.4 1,063,228 - -2.0 1,063,228 - -1.2 1,063,286 - -1.4
Or see this region's nucleotide sequence