Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1028 and ilvE are separated by 603 nucleotides ilvE and hisC are separated by 29 nucleotides
Synpcc7942_1028: Synpcc7942_1028 - hypothetical protein, at 1,042,384 to 1,043,340
_1028
Synpcc7942_1029: ilvE - branched-chain amino acid aminotransferase, at 1,043,944 to 1,044,858
ilvE
Synpcc7942_1030: hisC - histidinol-phosphate aminotransferase, at 1,044,888 to 1,046,009
hisC
Position (kb)
1043
1044
1045 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1042.973 kb on + strand, within Synpcc7942_1028 at 1042.973 kb on + strand, within Synpcc7942_1028 at 1042.993 kb on - strand, within Synpcc7942_1028 at 1043.008 kb on - strand, within Synpcc7942_1028 at 1043.027 kb on - strand, within Synpcc7942_1028 at 1043.031 kb on + strand, within Synpcc7942_1028 at 1043.039 kb on - strand, within Synpcc7942_1028 at 1043.039 kb on - strand, within Synpcc7942_1028 at 1043.054 kb on + strand, within Synpcc7942_1028 at 1043.067 kb on + strand, within Synpcc7942_1028 at 1043.067 kb on + strand, within Synpcc7942_1028 at 1043.075 kb on - strand, within Synpcc7942_1028 at 1043.075 kb on - strand, within Synpcc7942_1028 at 1043.075 kb on - strand, within Synpcc7942_1028 at 1043.075 kb on - strand, within Synpcc7942_1028 at 1043.075 kb on - strand, within Synpcc7942_1028 at 1043.075 kb on - strand, within Synpcc7942_1028 at 1043.075 kb on - strand, within Synpcc7942_1028 at 1043.075 kb on - strand, within Synpcc7942_1028 at 1043.082 kb on + strand at 1043.085 kb on - strand, within Synpcc7942_1028 at 1043.097 kb on - strand, within Synpcc7942_1028 at 1043.099 kb on + strand, within Synpcc7942_1028 at 1043.099 kb on + strand, within Synpcc7942_1028 at 1043.212 kb on + strand, within Synpcc7942_1028 at 1043.220 kb on - strand, within Synpcc7942_1028 at 1043.233 kb on + strand, within Synpcc7942_1028 at 1043.236 kb on - strand, within Synpcc7942_1028 at 1043.236 kb on - strand, within Synpcc7942_1028 at 1043.236 kb on - strand, within Synpcc7942_1028 at 1043.241 kb on - strand, within Synpcc7942_1028 at 1043.257 kb on + strand at 1043.260 kb on - strand at 1043.272 kb on + strand at 1043.280 kb on - strand at 1043.301 kb on + strand at 1043.351 kb on - strand at 1043.352 kb on - strand at 1043.421 kb on + strand at 1043.440 kb on - strand at 1043.499 kb on + strand at 1043.507 kb on - strand at 1043.547 kb on + strand at 1043.559 kb on + strand at 1043.559 kb on + strand at 1043.559 kb on + strand at 1043.559 kb on + strand at 1043.559 kb on + strand at 1043.567 kb on - strand at 1043.640 kb on - strand at 1043.645 kb on + strand at 1043.653 kb on - strand at 1043.653 kb on - strand at 1043.702 kb on + strand at 1043.710 kb on - strand at 1043.710 kb on - strand at 1043.710 kb on - strand at 1043.729 kb on - strand at 1043.748 kb on + strand at 1043.754 kb on - strand at 1043.769 kb on + strand at 1043.769 kb on + strand at 1043.779 kb on + strand at 1043.779 kb on + strand at 1043.779 kb on + strand at 1043.779 kb on + strand at 1043.779 kb on + strand at 1043.779 kb on + strand at 1043.787 kb on - strand at 1043.787 kb on - strand at 1043.800 kb on + strand at 1043.805 kb on + strand at 1043.808 kb on - strand at 1043.813 kb on - strand at 1043.823 kb on - strand at 1044.469 kb on - strand, within ilvE at 1044.520 kb on + strand, within ilvE at 1044.546 kb on - strand, within ilvE at 1044.627 kb on - strand, within ilvE at 1044.680 kb on - strand, within ilvE at 1044.688 kb on + strand, within ilvE at 1044.743 kb on + strand, within ilvE at 1044.746 kb on - strand, within ilvE at 1044.751 kb on - strand, within ilvE at 1044.757 kb on - strand, within ilvE at 1044.828 kb on + strand at 1044.877 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,042,973 + Synpcc7942_1028 0.62 +0.6 1,042,973 + Synpcc7942_1028 0.62 +3.7 1,042,993 - Synpcc7942_1028 0.64 +1.5 1,043,008 - Synpcc7942_1028 0.65 -0.9 1,043,027 - Synpcc7942_1028 0.67 +1.8 1,043,031 + Synpcc7942_1028 0.68 -2.0 1,043,039 - Synpcc7942_1028 0.68 +0.5 1,043,039 - Synpcc7942_1028 0.68 -2.6 1,043,054 + Synpcc7942_1028 0.70 -0.7 1,043,067 + Synpcc7942_1028 0.71 -0.1 1,043,067 + Synpcc7942_1028 0.71 +0.6 1,043,075 - Synpcc7942_1028 0.72 +3.8 1,043,075 - Synpcc7942_1028 0.72 -0.1 1,043,075 - Synpcc7942_1028 0.72 +1.0 1,043,075 - Synpcc7942_1028 0.72 +0.4 1,043,075 - Synpcc7942_1028 0.72 +2.3 1,043,075 - Synpcc7942_1028 0.72 +1.7 1,043,075 - Synpcc7942_1028 0.72 -0.8 1,043,075 - Synpcc7942_1028 0.72 +1.0 1,043,082 + +1.1 1,043,085 - Synpcc7942_1028 0.73 +1.1 1,043,097 - Synpcc7942_1028 0.75 -1.8 1,043,099 + Synpcc7942_1028 0.75 +1.0 1,043,099 + Synpcc7942_1028 0.75 -0.4 1,043,212 + Synpcc7942_1028 0.87 -1.3 1,043,220 - Synpcc7942_1028 0.87 -0.1 1,043,233 + Synpcc7942_1028 0.89 +1.2 1,043,236 - Synpcc7942_1028 0.89 -0.9 1,043,236 - Synpcc7942_1028 0.89 +0.6 1,043,236 - Synpcc7942_1028 0.89 +2.1 1,043,241 - Synpcc7942_1028 0.90 -0.6 1,043,257 + +0.6 1,043,260 - -1.5 1,043,272 + +0.8 1,043,280 - -0.8 1,043,301 + -0.9 1,043,351 - +0.7 1,043,352 - +1.6 1,043,421 + +0.5 1,043,440 - -1.3 1,043,499 + +1.3 1,043,507 - -0.5 1,043,547 + -1.1 1,043,559 + -2.8 1,043,559 + +1.7 1,043,559 + +1.1 1,043,559 + -0.2 1,043,559 + -3.5 1,043,567 - +2.5 1,043,640 - -2.0 1,043,645 + +0.5 1,043,653 - -1.4 1,043,653 - -0.6 1,043,702 + -0.9 1,043,710 - +4.9 1,043,710 - +1.5 1,043,710 - +0.6 1,043,729 - -2.1 1,043,748 + -2.3 1,043,754 - -0.2 1,043,769 + -0.9 1,043,769 + +1.1 1,043,779 + -0.6 1,043,779 + -2.3 1,043,779 + +0.1 1,043,779 + -2.6 1,043,779 + -2.3 1,043,779 + +0.6 1,043,787 - -1.3 1,043,787 - -0.5 1,043,800 + -0.5 1,043,805 + -0.8 1,043,808 - -0.3 1,043,813 - -1.4 1,043,823 - -3.6 1,044,469 - ilvE Synpcc7942_1029 0.57 -1.3 1,044,520 + ilvE Synpcc7942_1029 0.63 -1.6 1,044,546 - ilvE Synpcc7942_1029 0.66 -2.5 1,044,627 - ilvE Synpcc7942_1029 0.75 -2.3 1,044,680 - ilvE Synpcc7942_1029 0.80 -0.8 1,044,688 + ilvE Synpcc7942_1029 0.81 +1.6 1,044,743 + ilvE Synpcc7942_1029 0.87 -2.0 1,044,746 - ilvE Synpcc7942_1029 0.88 -1.3 1,044,751 - ilvE Synpcc7942_1029 0.88 -1.4 1,044,757 - ilvE Synpcc7942_1029 0.89 -2.1 1,044,828 + -1.5 1,044,877 - +0.1
Or see this region's nucleotide sequence