Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ugd and Synpcc7942_0974 are separated by 36 nucleotides Synpcc7942_0974 and mraW are separated by 116 nucleotides mraW and Synpcc7942_0976 are separated by 23 nucleotides
Synpcc7942_0973: ugd - UDP-glucose 6-dehydrogenase, at 979,758 to 980,930
ugd
Synpcc7942_0974: Synpcc7942_0974 - UDP-glucose dehydrogenase, at 980,967 to 981,137
_0974
Synpcc7942_0975: mraW - S-adenosyl-methyltransferase MraW, at 981,254 to 982,144
mraW
Synpcc7942_0976: Synpcc7942_0976 - CBS, at 982,168 to 984,897
_0976
Position (kb) 981
982
983 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 980.271 kb on - strand, within ugd at 980.274 kb on + strand, within ugd at 980.277 kb on - strand, within ugd at 980.279 kb on + strand, within ugd at 980.318 kb on + strand, within ugd at 980.353 kb on - strand, within ugd at 980.362 kb on + strand, within ugd at 980.362 kb on + strand, within ugd at 980.370 kb on - strand, within ugd at 980.433 kb on + strand, within ugd at 980.440 kb on + strand, within ugd at 980.453 kb on - strand, within ugd at 980.463 kb on - strand, within ugd at 980.472 kb on + strand, within ugd at 980.527 kb on + strand, within ugd at 980.534 kb on - strand, within ugd at 980.562 kb on + strand, within ugd at 980.562 kb on + strand, within ugd at 980.570 kb on - strand, within ugd at 980.570 kb on - strand, within ugd at 980.581 kb on + strand, within ugd at 980.590 kb on - strand, within ugd at 980.629 kb on + strand, within ugd at 980.637 kb on - strand, within ugd at 980.639 kb on + strand, within ugd at 980.639 kb on + strand, within ugd at 980.647 kb on - strand, within ugd at 980.647 kb on - strand, within ugd at 980.657 kb on - strand, within ugd at 980.697 kb on - strand, within ugd at 980.744 kb on - strand, within ugd at 980.746 kb on - strand, within ugd at 980.782 kb on + strand, within ugd at 980.805 kb on + strand, within ugd at 980.821 kb on - strand at 980.837 kb on - strand at 980.855 kb on - strand at 980.855 kb on - strand at 980.868 kb on + strand at 980.912 kb on - strand at 980.924 kb on + strand at 980.930 kb on + strand at 980.930 kb on + strand at 980.935 kb on + strand at 980.941 kb on + strand at 980.949 kb on - strand at 981.024 kb on - strand, within Synpcc7942_0974 at 981.077 kb on + strand, within Synpcc7942_0974 at 981.123 kb on - strand at 981.123 kb on - strand at 981.127 kb on - strand at 981.164 kb on + strand at 981.166 kb on + strand at 981.200 kb on + strand at 981.207 kb on + strand at 981.208 kb on - strand at 981.209 kb on - strand at 981.212 kb on + strand at 981.213 kb on + strand at 981.215 kb on - strand at 981.215 kb on - strand at 981.215 kb on - strand at 981.220 kb on - strand at 981.232 kb on - strand at 981.232 kb on - strand at 981.232 kb on - strand at 981.247 kb on - strand at 981.247 kb on - strand at 981.252 kb on + strand at 981.260 kb on - strand at 981.343 kb on - strand, within mraW at 981.347 kb on + strand, within mraW at 981.371 kb on - strand, within mraW at 981.375 kb on + strand, within mraW at 981.398 kb on + strand, within mraW at 981.406 kb on + strand, within mraW at 981.406 kb on + strand, within mraW at 981.425 kb on + strand, within mraW at 981.430 kb on + strand, within mraW at 981.470 kb on + strand, within mraW at 981.479 kb on + strand, within mraW at 981.488 kb on - strand, within mraW at 981.510 kb on + strand, within mraW at 981.522 kb on + strand, within mraW at 981.522 kb on + strand, within mraW at 981.530 kb on - strand, within mraW at 981.530 kb on - strand, within mraW at 981.579 kb on + strand, within mraW at 981.601 kb on + strand, within mraW at 981.601 kb on + strand, within mraW at 981.609 kb on - strand, within mraW at 981.609 kb on - strand, within mraW at 981.643 kb on + strand, within mraW at 981.651 kb on - strand, within mraW at 981.804 kb on + strand, within mraW at 981.804 kb on + strand, within mraW at 981.804 kb on + strand, within mraW at 981.808 kb on + strand, within mraW at 981.812 kb on - strand, within mraW at 981.829 kb on + strand, within mraW at 981.878 kb on - strand, within mraW at 981.879 kb on - strand, within mraW at 981.894 kb on + strand, within mraW at 981.923 kb on - strand, within mraW at 981.923 kb on - strand, within mraW at 981.939 kb on - strand, within mraW at 981.940 kb on - strand, within mraW at 981.966 kb on - strand, within mraW at 981.984 kb on + strand, within mraW at 981.997 kb on - strand, within mraW at 981.997 kb on - strand, within mraW at 982.028 kb on + strand, within mraW at 982.067 kb on + strand at 982.093 kb on + strand at 982.096 kb on + strand at 982.098 kb on + strand at 982.099 kb on - strand at 982.154 kb on + strand at 982.162 kb on - strand at 982.162 kb on - strand at 982.166 kb on + strand at 982.758 kb on + strand, within Synpcc7942_0976 at 982.766 kb on - strand, within Synpcc7942_0976 at 982.766 kb on - strand, within Synpcc7942_0976
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 980,271 - ugd Synpcc7942_0973 0.44 -0.8 980,274 + ugd Synpcc7942_0973 0.44 -2.2 980,277 - ugd Synpcc7942_0973 0.44 -3.6 980,279 + ugd Synpcc7942_0973 0.44 -1.0 980,318 + ugd Synpcc7942_0973 0.48 +0.1 980,353 - ugd Synpcc7942_0973 0.51 +0.8 980,362 + ugd Synpcc7942_0973 0.51 +0.1 980,362 + ugd Synpcc7942_0973 0.51 -2.0 980,370 - ugd Synpcc7942_0973 0.52 +1.1 980,433 + ugd Synpcc7942_0973 0.58 -2.8 980,440 + ugd Synpcc7942_0973 0.58 -1.9 980,453 - ugd Synpcc7942_0973 0.59 -1.5 980,463 - ugd Synpcc7942_0973 0.60 +0.1 980,472 + ugd Synpcc7942_0973 0.61 -2.0 980,527 + ugd Synpcc7942_0973 0.66 +1.1 980,534 - ugd Synpcc7942_0973 0.66 -0.4 980,562 + ugd Synpcc7942_0973 0.69 +0.5 980,562 + ugd Synpcc7942_0973 0.69 -1.0 980,570 - ugd Synpcc7942_0973 0.69 -0.6 980,570 - ugd Synpcc7942_0973 0.69 -1.9 980,581 + ugd Synpcc7942_0973 0.70 -1.6 980,590 - ugd Synpcc7942_0973 0.71 -0.4 980,629 + ugd Synpcc7942_0973 0.74 +0.3 980,637 - ugd Synpcc7942_0973 0.75 -1.1 980,639 + ugd Synpcc7942_0973 0.75 +2.9 980,639 + ugd Synpcc7942_0973 0.75 -0.5 980,647 - ugd Synpcc7942_0973 0.76 -3.0 980,647 - ugd Synpcc7942_0973 0.76 +1.9 980,657 - ugd Synpcc7942_0973 0.77 -1.9 980,697 - ugd Synpcc7942_0973 0.80 -0.5 980,744 - ugd Synpcc7942_0973 0.84 +0.6 980,746 - ugd Synpcc7942_0973 0.84 -1.9 980,782 + ugd Synpcc7942_0973 0.87 +2.2 980,805 + ugd Synpcc7942_0973 0.89 +3.3 980,821 - -1.3 980,837 - +0.5 980,855 - -0.3 980,855 - -0.5 980,868 + +0.6 980,912 - +1.2 980,924 + +0.5 980,930 + -1.0 980,930 + +0.5 980,935 + -0.3 980,941 + -2.2 980,949 - -1.3 981,024 - Synpcc7942_0974 0.33 +0.1 981,077 + Synpcc7942_0974 0.64 +1.4 981,123 - -2.0 981,123 - -1.3 981,127 - +1.2 981,164 + +1.0 981,166 + -1.8 981,200 + +1.8 981,207 + -0.3 981,208 - -0.2 981,209 - -3.1 981,212 + -0.6 981,213 + +2.2 981,215 - +0.5 981,215 - -0.8 981,215 - -0.5 981,220 - -1.9 981,232 - +1.0 981,232 - +1.2 981,232 - -0.9 981,247 - +1.6 981,247 - +2.1 981,252 + +3.8 981,260 - +0.2 981,343 - mraW Synpcc7942_0975 0.10 -1.7 981,347 + mraW Synpcc7942_0975 0.10 -2.9 981,371 - mraW Synpcc7942_0975 0.13 -1.9 981,375 + mraW Synpcc7942_0975 0.14 -0.8 981,398 + mraW Synpcc7942_0975 0.16 -1.3 981,406 + mraW Synpcc7942_0975 0.17 -2.9 981,406 + mraW Synpcc7942_0975 0.17 -2.9 981,425 + mraW Synpcc7942_0975 0.19 -4.4 981,430 + mraW Synpcc7942_0975 0.20 -2.5 981,470 + mraW Synpcc7942_0975 0.24 -2.6 981,479 + mraW Synpcc7942_0975 0.25 -1.8 981,488 - mraW Synpcc7942_0975 0.26 -2.0 981,510 + mraW Synpcc7942_0975 0.29 -3.0 981,522 + mraW Synpcc7942_0975 0.30 -1.0 981,522 + mraW Synpcc7942_0975 0.30 -3.1 981,530 - mraW Synpcc7942_0975 0.31 -4.8 981,530 - mraW Synpcc7942_0975 0.31 -3.6 981,579 + mraW Synpcc7942_0975 0.36 -3.6 981,601 + mraW Synpcc7942_0975 0.39 -0.8 981,601 + mraW Synpcc7942_0975 0.39 -2.3 981,609 - mraW Synpcc7942_0975 0.40 -4.8 981,609 - mraW Synpcc7942_0975 0.40 -1.1 981,643 + mraW Synpcc7942_0975 0.44 -3.3 981,651 - mraW Synpcc7942_0975 0.45 -1.5 981,804 + mraW Synpcc7942_0975 0.62 -1.0 981,804 + mraW Synpcc7942_0975 0.62 -1.6 981,804 + mraW Synpcc7942_0975 0.62 -3.0 981,808 + mraW Synpcc7942_0975 0.62 -2.3 981,812 - mraW Synpcc7942_0975 0.63 -0.5 981,829 + mraW Synpcc7942_0975 0.65 -3.9 981,878 - mraW Synpcc7942_0975 0.70 -2.4 981,879 - mraW Synpcc7942_0975 0.70 -4.1 981,894 + mraW Synpcc7942_0975 0.72 -3.7 981,923 - mraW Synpcc7942_0975 0.75 -1.7 981,923 - mraW Synpcc7942_0975 0.75 -2.9 981,939 - mraW Synpcc7942_0975 0.77 +0.0 981,940 - mraW Synpcc7942_0975 0.77 -1.3 981,966 - mraW Synpcc7942_0975 0.80 -3.0 981,984 + mraW Synpcc7942_0975 0.82 -1.9 981,997 - mraW Synpcc7942_0975 0.83 -3.9 981,997 - mraW Synpcc7942_0975 0.83 -2.2 982,028 + mraW Synpcc7942_0975 0.87 -1.0 982,067 + -2.2 982,093 + -1.0 982,096 + -3.6 982,098 + -2.5 982,099 - -1.9 982,154 + +1.0 982,162 - -1.9 982,162 - -1.7 982,166 + +0.3 982,758 + Synpcc7942_0976 0.22 +2.2 982,766 - Synpcc7942_0976 0.22 -2.5 982,766 - Synpcc7942_0976 0.22 -2.0
Or see this region's nucleotide sequence