Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemC and Synpcc7942_0968 are separated by 81 nucleotides Synpcc7942_0968 and Synpcc7942_0969 are separated by 9 nucleotides Synpcc7942_0969 and crtD are separated by 115 nucleotides
Synpcc7942_0967: hemC - porphobilinogen deaminase, at 974,099 to 975,061
hemC
Synpcc7942_0968: Synpcc7942_0968 - hypothetical protein, at 975,143 to 975,358
_0968
Synpcc7942_0969: Synpcc7942_0969 - Carboxymethylenebutenolidase, at 975,368 to 976,195
_0969
Synpcc7942_0970: crtD - phytoene dehydrogenase-like, at 976,311 to 977,882
crtD
Position (kb) 975
976 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 975.116 kb on + strand at 975.116 kb on + strand at 975.116 kb on + strand at 975.126 kb on + strand at 975.334 kb on - strand, within Synpcc7942_0968 at 975.382 kb on + strand at 975.382 kb on + strand at 975.382 kb on + strand at 975.382 kb on + strand at 975.390 kb on - strand at 975.393 kb on + strand at 975.437 kb on - strand at 975.441 kb on + strand at 975.451 kb on - strand, within Synpcc7942_0969 at 975.461 kb on + strand, within Synpcc7942_0969 at 975.508 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.530 kb on + strand, within Synpcc7942_0969 at 975.538 kb on - strand, within Synpcc7942_0969 at 975.554 kb on + strand, within Synpcc7942_0969 at 975.555 kb on + strand, within Synpcc7942_0969 at 975.555 kb on + strand, within Synpcc7942_0969 at 975.562 kb on - strand, within Synpcc7942_0969 at 975.563 kb on - strand, within Synpcc7942_0969 at 975.578 kb on + strand, within Synpcc7942_0969 at 975.591 kb on - strand, within Synpcc7942_0969 at 975.608 kb on + strand, within Synpcc7942_0969 at 975.618 kb on + strand, within Synpcc7942_0969 at 975.630 kb on - strand, within Synpcc7942_0969 at 975.634 kb on + strand, within Synpcc7942_0969 at 975.662 kb on + strand, within Synpcc7942_0969 at 975.686 kb on - strand, within Synpcc7942_0969 at 975.702 kb on + strand, within Synpcc7942_0969 at 975.702 kb on + strand, within Synpcc7942_0969 at 975.721 kb on + strand, within Synpcc7942_0969 at 975.731 kb on + strand, within Synpcc7942_0969 at 975.767 kb on - strand, within Synpcc7942_0969 at 975.782 kb on - strand, within Synpcc7942_0969 at 975.833 kb on + strand, within Synpcc7942_0969 at 975.898 kb on + strand, within Synpcc7942_0969 at 975.898 kb on + strand, within Synpcc7942_0969 at 975.906 kb on - strand, within Synpcc7942_0969 at 975.925 kb on + strand, within Synpcc7942_0969 at 975.947 kb on + strand, within Synpcc7942_0969 at 976.038 kb on + strand, within Synpcc7942_0969 at 976.043 kb on - strand, within Synpcc7942_0969 at 976.053 kb on - strand, within Synpcc7942_0969 at 976.078 kb on + strand, within Synpcc7942_0969 at 976.087 kb on - strand, within Synpcc7942_0969 at 976.087 kb on - strand, within Synpcc7942_0969 at 976.098 kb on + strand, within Synpcc7942_0969 at 976.130 kb on + strand at 976.135 kb on + strand at 976.135 kb on + strand at 976.154 kb on - strand at 976.162 kb on - strand at 976.206 kb on - strand at 976.206 kb on - strand at 976.206 kb on - strand at 976.206 kb on - strand at 976.206 kb on - strand at 976.254 kb on + strand at 976.262 kb on - strand at 976.314 kb on + strand at 976.323 kb on - strand at 976.325 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 975,116 + +1.6 975,116 + +0.0 975,116 + +1.3 975,126 + -0.6 975,334 - Synpcc7942_0968 0.88 -1.9 975,382 + -1.1 975,382 + +1.8 975,382 + -0.5 975,382 + -1.3 975,390 - +2.1 975,393 + +2.2 975,437 - +1.3 975,441 + -0.7 975,451 - Synpcc7942_0969 0.10 -0.6 975,461 + Synpcc7942_0969 0.11 -2.6 975,508 - Synpcc7942_0969 0.17 +2.3 975,518 - Synpcc7942_0969 0.18 -2.0 975,518 - Synpcc7942_0969 0.18 +0.5 975,518 - Synpcc7942_0969 0.18 +1.3 975,518 - Synpcc7942_0969 0.18 +4.1 975,518 - Synpcc7942_0969 0.18 +0.8 975,518 - Synpcc7942_0969 0.18 -0.3 975,530 + Synpcc7942_0969 0.20 +0.1 975,538 - Synpcc7942_0969 0.21 +0.3 975,554 + Synpcc7942_0969 0.22 -2.4 975,555 + Synpcc7942_0969 0.23 +0.6 975,555 + Synpcc7942_0969 0.23 -1.0 975,562 - Synpcc7942_0969 0.23 +0.7 975,563 - Synpcc7942_0969 0.24 +2.9 975,578 + Synpcc7942_0969 0.25 +0.4 975,591 - Synpcc7942_0969 0.27 -0.7 975,608 + Synpcc7942_0969 0.29 +0.7 975,618 + Synpcc7942_0969 0.30 -0.2 975,630 - Synpcc7942_0969 0.32 -0.6 975,634 + Synpcc7942_0969 0.32 +0.2 975,662 + Synpcc7942_0969 0.36 -0.4 975,686 - Synpcc7942_0969 0.38 -2.7 975,702 + Synpcc7942_0969 0.40 +3.0 975,702 + Synpcc7942_0969 0.40 +1.6 975,721 + Synpcc7942_0969 0.43 +1.5 975,731 + Synpcc7942_0969 0.44 +1.3 975,767 - Synpcc7942_0969 0.48 -0.5 975,782 - Synpcc7942_0969 0.50 +5.4 975,833 + Synpcc7942_0969 0.56 +0.7 975,898 + Synpcc7942_0969 0.64 -0.5 975,898 + Synpcc7942_0969 0.64 -2.4 975,906 - Synpcc7942_0969 0.65 -0.0 975,925 + Synpcc7942_0969 0.67 -2.3 975,947 + Synpcc7942_0969 0.70 -0.5 976,038 + Synpcc7942_0969 0.81 +1.1 976,043 - Synpcc7942_0969 0.82 +4.1 976,053 - Synpcc7942_0969 0.83 +1.2 976,078 + Synpcc7942_0969 0.86 +0.2 976,087 - Synpcc7942_0969 0.87 +0.5 976,087 - Synpcc7942_0969 0.87 +0.4 976,098 + Synpcc7942_0969 0.88 +0.7 976,130 + +1.5 976,135 + -0.2 976,135 + +1.2 976,154 - +1.0 976,162 - +0.4 976,206 - +0.2 976,206 - -2.0 976,206 - -0.9 976,206 - +2.8 976,206 - -0.8 976,254 + +1.8 976,262 - +2.5 976,314 + -1.0 976,323 - -2.8 976,325 + +1.3
Or see this region's nucleotide sequence