Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0963 and Synpcc7942_0964 are separated by 216 nucleotides Synpcc7942_0964 and amt1 are separated by 13 nucleotides amt1 and Synpcc7942_0966 are separated by 8 nucleotides Synpcc7942_0966 and hemC are separated by 152 nucleotides
Synpcc7942_0963: Synpcc7942_0963 - probable methyltransferase, at 971,259 to 971,639
_0963
Synpcc7942_0964: Synpcc7942_0964 - hypothetical protein, at 971,856 to 972,374
_0964
Synpcc7942_0965: amt1 - ammonium transporter protein Amt1-like, at 972,388 to 973,350
amt1
Synpcc7942_0966: Synpcc7942_0966 - hypothetical protein, at 973,359 to 973,946
_0966
Synpcc7942_0967: hemC - porphobilinogen deaminase, at 974,099 to 975,061
hemC
Position (kb) 972
973
974 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 971.392 kb on - strand at 971.435 kb on - strand, within Synpcc7942_0963 at 971.439 kb on + strand, within Synpcc7942_0963 at 971.443 kb on - strand at 971.447 kb on - strand, within Synpcc7942_0963 at 971.447 kb on - strand, within Synpcc7942_0963 at 971.466 kb on - strand, within Synpcc7942_0963 at 971.485 kb on + strand, within Synpcc7942_0963 at 971.495 kb on + strand, within Synpcc7942_0963 at 971.495 kb on + strand, within Synpcc7942_0963 at 971.495 kb on + strand, within Synpcc7942_0963 at 971.503 kb on - strand, within Synpcc7942_0963 at 971.503 kb on - strand at 971.504 kb on + strand, within Synpcc7942_0963 at 971.514 kb on - strand, within Synpcc7942_0963 at 971.514 kb on - strand, within Synpcc7942_0963 at 971.523 kb on + strand, within Synpcc7942_0963 at 971.547 kb on - strand, within Synpcc7942_0963 at 971.548 kb on + strand, within Synpcc7942_0963 at 971.569 kb on + strand, within Synpcc7942_0963 at 971.592 kb on - strand, within Synpcc7942_0963 at 971.592 kb on - strand, within Synpcc7942_0963 at 971.614 kb on + strand at 971.647 kb on - strand at 971.647 kb on - strand at 971.702 kb on - strand at 971.702 kb on - strand at 971.707 kb on - strand at 971.721 kb on + strand at 971.721 kb on + strand at 971.729 kb on - strand at 971.762 kb on + strand at 971.770 kb on - strand at 971.808 kb on + strand at 971.811 kb on - strand at 971.848 kb on - strand at 971.855 kb on + strand at 971.907 kb on - strand at 971.907 kb on - strand at 971.907 kb on - strand at 971.929 kb on - strand, within Synpcc7942_0964 at 971.945 kb on + strand, within Synpcc7942_0964 at 971.957 kb on + strand, within Synpcc7942_0964 at 971.979 kb on + strand, within Synpcc7942_0964 at 972.002 kb on + strand, within Synpcc7942_0964 at 972.009 kb on + strand, within Synpcc7942_0964 at 972.014 kb on + strand, within Synpcc7942_0964 at 972.014 kb on + strand, within Synpcc7942_0964 at 972.014 kb on + strand, within Synpcc7942_0964 at 972.014 kb on + strand, within Synpcc7942_0964 at 972.014 kb on + strand, within Synpcc7942_0964 at 972.014 kb on - strand, within Synpcc7942_0964 at 972.022 kb on - strand, within Synpcc7942_0964 at 972.022 kb on - strand, within Synpcc7942_0964 at 972.031 kb on + strand, within Synpcc7942_0964 at 972.031 kb on + strand, within Synpcc7942_0964 at 972.039 kb on - strand, within Synpcc7942_0964 at 972.048 kb on + strand, within Synpcc7942_0964 at 972.056 kb on - strand, within Synpcc7942_0964 at 972.056 kb on - strand, within Synpcc7942_0964 at 972.089 kb on + strand, within Synpcc7942_0964 at 972.089 kb on + strand, within Synpcc7942_0964 at 972.089 kb on + strand, within Synpcc7942_0964 at 972.095 kb on + strand, within Synpcc7942_0964 at 972.097 kb on - strand, within Synpcc7942_0964 at 972.097 kb on - strand, within Synpcc7942_0964 at 972.097 kb on - strand, within Synpcc7942_0964 at 972.098 kb on - strand, within Synpcc7942_0964 at 972.103 kb on - strand, within Synpcc7942_0964 at 972.105 kb on + strand, within Synpcc7942_0964 at 972.132 kb on + strand, within Synpcc7942_0964 at 972.134 kb on + strand, within Synpcc7942_0964 at 972.148 kb on - strand, within Synpcc7942_0964 at 972.159 kb on + strand, within Synpcc7942_0964 at 972.159 kb on + strand, within Synpcc7942_0964 at 972.159 kb on + strand, within Synpcc7942_0964 at 972.167 kb on - strand, within Synpcc7942_0964 at 972.172 kb on + strand, within Synpcc7942_0964 at 972.172 kb on + strand, within Synpcc7942_0964 at 972.172 kb on + strand, within Synpcc7942_0964 at 972.180 kb on - strand, within Synpcc7942_0964 at 972.180 kb on - strand, within Synpcc7942_0964 at 972.217 kb on + strand, within Synpcc7942_0964 at 972.241 kb on + strand, within Synpcc7942_0964 at 972.265 kb on - strand, within Synpcc7942_0964 at 972.318 kb on + strand, within Synpcc7942_0964 at 972.325 kb on - strand at 972.325 kb on - strand at 972.374 kb on + strand at 972.376 kb on - strand at 972.376 kb on - strand at 972.380 kb on + strand at 972.385 kb on + strand at 972.392 kb on - strand at 972.407 kb on + strand at 972.407 kb on + strand at 972.407 kb on + strand at 972.407 kb on + strand at 972.415 kb on - strand at 972.424 kb on + strand at 972.424 kb on + strand at 972.432 kb on - strand at 972.446 kb on + strand at 972.458 kb on + strand at 972.458 kb on + strand at 972.466 kb on - strand at 972.486 kb on - strand, within amt1 at 972.495 kb on + strand, within amt1 at 972.495 kb on + strand, within amt1 at 972.498 kb on - strand, within amt1 at 972.502 kb on + strand, within amt1 at 972.503 kb on - strand, within amt1 at 972.503 kb on - strand, within amt1 at 972.529 kb on - strand, within amt1 at 972.583 kb on + strand, within amt1 at 972.596 kb on + strand, within amt1 at 972.633 kb on + strand, within amt1 at 972.709 kb on + strand, within amt1 at 972.723 kb on - strand, within amt1 at 972.732 kb on + strand, within amt1 at 972.740 kb on - strand, within amt1 at 972.766 kb on + strand, within amt1 at 972.821 kb on - strand, within amt1 at 972.830 kb on - strand, within amt1 at 972.830 kb on - strand, within amt1 at 972.835 kb on - strand, within amt1 at 972.867 kb on - strand, within amt1 at 972.872 kb on - strand, within amt1 at 972.894 kb on - strand, within amt1 at 972.899 kb on + strand at 972.924 kb on + strand, within amt1 at 972.956 kb on - strand, within amt1 at 972.958 kb on + strand, within amt1 at 972.959 kb on + strand, within amt1 at 972.961 kb on + strand, within amt1 at 972.980 kb on + strand, within amt1 at 972.988 kb on - strand, within amt1 at 973.004 kb on + strand, within amt1 at 973.006 kb on - strand, within amt1 at 973.050 kb on + strand, within amt1 at 973.068 kb on - strand, within amt1 at 973.084 kb on + strand, within amt1 at 973.115 kb on - strand, within amt1 at 973.115 kb on - strand, within amt1 at 973.118 kb on - strand, within amt1 at 973.120 kb on - strand, within amt1 at 973.120 kb on - strand, within amt1 at 973.153 kb on + strand, within amt1 at 973.161 kb on - strand, within amt1 at 973.232 kb on + strand, within amt1 at 973.240 kb on - strand, within amt1 at 973.306 kb on - strand at 973.312 kb on + strand at 973.320 kb on + strand at 973.324 kb on + strand at 973.345 kb on + strand at 973.352 kb on - strand at 973.352 kb on - strand at 973.357 kb on + strand at 973.357 kb on + strand at 973.357 kb on + strand at 973.357 kb on + strand at 973.357 kb on + strand at 973.357 kb on + strand at 973.362 kb on + strand at 973.365 kb on - strand at 973.380 kb on - strand at 973.382 kb on + strand at 973.385 kb on - strand at 973.388 kb on - strand at 973.401 kb on - strand at 973.401 kb on - strand at 973.406 kb on - strand at 973.493 kb on + strand, within Synpcc7942_0966 at 973.524 kb on - strand, within Synpcc7942_0966 at 973.524 kb on - strand, within Synpcc7942_0966 at 973.551 kb on - strand, within Synpcc7942_0966 at 973.573 kb on + strand, within Synpcc7942_0966 at 973.575 kb on - strand, within Synpcc7942_0966 at 973.592 kb on + strand, within Synpcc7942_0966 at 973.592 kb on + strand, within Synpcc7942_0966 at 973.592 kb on + strand, within Synpcc7942_0966 at 973.592 kb on + strand, within Synpcc7942_0966 at 973.592 kb on + strand, within Synpcc7942_0966 at 973.592 kb on + strand, within Synpcc7942_0966 at 973.594 kb on + strand, within Synpcc7942_0966 at 973.600 kb on - strand, within Synpcc7942_0966 at 973.600 kb on - strand, within Synpcc7942_0966 at 973.602 kb on + strand, within Synpcc7942_0966 at 973.602 kb on - strand, within Synpcc7942_0966 at 973.607 kb on + strand, within Synpcc7942_0966 at 973.610 kb on - strand, within Synpcc7942_0966 at 973.612 kb on + strand, within Synpcc7942_0966 at 973.620 kb on - strand, within Synpcc7942_0966 at 973.630 kb on - strand, within Synpcc7942_0966 at 973.646 kb on - strand, within Synpcc7942_0966 at 973.653 kb on + strand, within Synpcc7942_0966 at 973.661 kb on - strand, within Synpcc7942_0966 at 973.695 kb on + strand, within Synpcc7942_0966 at 973.704 kb on - strand, within Synpcc7942_0966 at 973.706 kb on + strand, within Synpcc7942_0966 at 973.706 kb on + strand, within Synpcc7942_0966 at 973.714 kb on - strand, within Synpcc7942_0966 at 973.714 kb on - strand, within Synpcc7942_0966 at 973.714 kb on - strand, within Synpcc7942_0966 at 973.714 kb on - strand, within Synpcc7942_0966 at 973.716 kb on + strand, within Synpcc7942_0966 at 973.716 kb on - strand, within Synpcc7942_0966 at 973.716 kb on - strand, within Synpcc7942_0966 at 973.716 kb on - strand, within Synpcc7942_0966 at 973.720 kb on + strand, within Synpcc7942_0966 at 973.724 kb on - strand, within Synpcc7942_0966 at 973.728 kb on - strand, within Synpcc7942_0966 at 973.811 kb on + strand, within Synpcc7942_0966 at 973.817 kb on - strand, within Synpcc7942_0966 at 973.824 kb on - strand, within Synpcc7942_0966 at 973.824 kb on - strand, within Synpcc7942_0966 at 973.908 kb on - strand at 973.932 kb on - strand at 973.932 kb on - strand at 973.932 kb on - strand at 973.932 kb on - strand at 973.959 kb on + strand at 973.959 kb on + strand at 973.959 kb on + strand at 973.959 kb on + strand at 973.964 kb on + strand at 973.964 kb on + strand at 973.967 kb on - strand at 973.967 kb on - strand at 973.967 kb on - strand at 973.971 kb on + strand at 973.971 kb on + strand at 973.972 kb on + strand at 973.972 kb on - strand at 973.979 kb on - strand at 973.987 kb on + strand at 973.996 kb on - strand at 973.996 kb on - strand at 974.000 kb on - strand at 974.000 kb on - strand at 974.097 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 971,392 - +1.7 971,435 - Synpcc7942_0963 0.46 -2.6 971,439 + Synpcc7942_0963 0.47 -0.1 971,443 - -0.5 971,447 - Synpcc7942_0963 0.49 +1.5 971,447 - Synpcc7942_0963 0.49 -0.2 971,466 - Synpcc7942_0963 0.54 +1.3 971,485 + Synpcc7942_0963 0.59 -0.8 971,495 + Synpcc7942_0963 0.62 +0.0 971,495 + Synpcc7942_0963 0.62 +0.1 971,495 + Synpcc7942_0963 0.62 +0.8 971,503 - Synpcc7942_0963 0.64 +0.9 971,503 - -0.5 971,504 + Synpcc7942_0963 0.64 -3.2 971,514 - Synpcc7942_0963 0.67 -0.2 971,514 - Synpcc7942_0963 0.67 -1.5 971,523 + Synpcc7942_0963 0.69 -0.8 971,547 - Synpcc7942_0963 0.76 -0.6 971,548 + Synpcc7942_0963 0.76 -1.3 971,569 + Synpcc7942_0963 0.81 +0.8 971,592 - Synpcc7942_0963 0.87 -1.6 971,592 - Synpcc7942_0963 0.87 +0.1 971,614 + +5.6 971,647 - +2.5 971,647 - -2.0 971,702 - -2.4 971,702 - -3.6 971,707 - +0.1 971,721 + -1.0 971,721 + -1.7 971,729 - +1.8 971,762 + -0.5 971,770 - +1.0 971,808 + -0.8 971,811 - -1.6 971,848 - -2.8 971,855 + +3.8 971,907 - -2.4 971,907 - +3.2 971,907 - +2.2 971,929 - Synpcc7942_0964 0.14 +2.6 971,945 + Synpcc7942_0964 0.17 +2.7 971,957 + Synpcc7942_0964 0.19 -0.6 971,979 + Synpcc7942_0964 0.24 +1.3 972,002 + Synpcc7942_0964 0.28 +2.3 972,009 + Synpcc7942_0964 0.29 +1.0 972,014 + Synpcc7942_0964 0.30 +3.5 972,014 + Synpcc7942_0964 0.30 +1.4 972,014 + Synpcc7942_0964 0.30 +2.8 972,014 + Synpcc7942_0964 0.30 +0.9 972,014 + Synpcc7942_0964 0.30 +0.7 972,014 - Synpcc7942_0964 0.30 +1.4 972,022 - Synpcc7942_0964 0.32 -0.8 972,022 - Synpcc7942_0964 0.32 +1.7 972,031 + Synpcc7942_0964 0.34 -0.4 972,031 + Synpcc7942_0964 0.34 -1.9 972,039 - Synpcc7942_0964 0.35 +2.9 972,048 + Synpcc7942_0964 0.37 +2.2 972,056 - Synpcc7942_0964 0.39 -2.0 972,056 - Synpcc7942_0964 0.39 -0.2 972,089 + Synpcc7942_0964 0.45 +1.0 972,089 + Synpcc7942_0964 0.45 +0.3 972,089 + Synpcc7942_0964 0.45 +1.2 972,095 + Synpcc7942_0964 0.46 -2.3 972,097 - Synpcc7942_0964 0.46 -0.6 972,097 - Synpcc7942_0964 0.46 +1.8 972,097 - Synpcc7942_0964 0.46 +3.4 972,098 - Synpcc7942_0964 0.47 +0.4 972,103 - Synpcc7942_0964 0.48 -1.3 972,105 + Synpcc7942_0964 0.48 -0.8 972,132 + Synpcc7942_0964 0.53 +2.1 972,134 + Synpcc7942_0964 0.54 -2.4 972,148 - Synpcc7942_0964 0.56 +0.4 972,159 + Synpcc7942_0964 0.58 +3.1 972,159 + Synpcc7942_0964 0.58 +3.1 972,159 + Synpcc7942_0964 0.58 +3.2 972,167 - Synpcc7942_0964 0.60 -1.7 972,172 + Synpcc7942_0964 0.61 +0.1 972,172 + Synpcc7942_0964 0.61 -2.4 972,172 + Synpcc7942_0964 0.61 -2.0 972,180 - Synpcc7942_0964 0.62 +1.9 972,180 - Synpcc7942_0964 0.62 +1.4 972,217 + Synpcc7942_0964 0.70 -0.8 972,241 + Synpcc7942_0964 0.74 +1.4 972,265 - Synpcc7942_0964 0.79 +1.1 972,318 + Synpcc7942_0964 0.89 +1.0 972,325 - -0.3 972,325 - +2.9 972,374 + +0.4 972,376 - +1.6 972,376 - +0.8 972,380 + -0.9 972,385 + -0.8 972,392 - -2.0 972,407 + +3.3 972,407 + +1.3 972,407 + +1.7 972,407 + +1.3 972,415 - +0.0 972,424 + +4.4 972,424 + -0.2 972,432 - +1.3 972,446 + +1.4 972,458 + +2.2 972,458 + +1.8 972,466 - +1.7 972,486 - amt1 Synpcc7942_0965 0.10 +2.6 972,495 + amt1 Synpcc7942_0965 0.11 -2.0 972,495 + amt1 Synpcc7942_0965 0.11 +4.0 972,498 - amt1 Synpcc7942_0965 0.11 +1.5 972,502 + amt1 Synpcc7942_0965 0.12 +1.1 972,503 - amt1 Synpcc7942_0965 0.12 -0.3 972,503 - amt1 Synpcc7942_0965 0.12 +2.6 972,529 - amt1 Synpcc7942_0965 0.15 +1.3 972,583 + amt1 Synpcc7942_0965 0.20 +1.9 972,596 + amt1 Synpcc7942_0965 0.22 +2.0 972,633 + amt1 Synpcc7942_0965 0.25 +1.3 972,709 + amt1 Synpcc7942_0965 0.33 -3.5 972,723 - amt1 Synpcc7942_0965 0.35 +4.1 972,732 + amt1 Synpcc7942_0965 0.36 -1.5 972,740 - amt1 Synpcc7942_0965 0.37 +0.5 972,766 + amt1 Synpcc7942_0965 0.39 +3.3 972,821 - amt1 Synpcc7942_0965 0.45 -0.8 972,830 - amt1 Synpcc7942_0965 0.46 +1.5 972,830 - amt1 Synpcc7942_0965 0.46 +0.8 972,835 - amt1 Synpcc7942_0965 0.46 +1.1 972,867 - amt1 Synpcc7942_0965 0.50 +1.4 972,872 - amt1 Synpcc7942_0965 0.50 +0.1 972,894 - amt1 Synpcc7942_0965 0.53 +0.6 972,899 + -1.4 972,924 + amt1 Synpcc7942_0965 0.56 -2.6 972,956 - amt1 Synpcc7942_0965 0.59 +2.8 972,958 + amt1 Synpcc7942_0965 0.59 +2.7 972,959 + amt1 Synpcc7942_0965 0.59 +3.0 972,961 + amt1 Synpcc7942_0965 0.60 +0.8 972,980 + amt1 Synpcc7942_0965 0.61 -0.6 972,988 - amt1 Synpcc7942_0965 0.62 -3.8 973,004 + amt1 Synpcc7942_0965 0.64 +2.8 973,006 - amt1 Synpcc7942_0965 0.64 +1.3 973,050 + amt1 Synpcc7942_0965 0.69 +1.9 973,068 - amt1 Synpcc7942_0965 0.71 +1.0 973,084 + amt1 Synpcc7942_0965 0.72 +2.5 973,115 - amt1 Synpcc7942_0965 0.75 +2.3 973,115 - amt1 Synpcc7942_0965 0.75 +3.1 973,118 - amt1 Synpcc7942_0965 0.76 -3.1 973,120 - amt1 Synpcc7942_0965 0.76 +2.3 973,120 - amt1 Synpcc7942_0965 0.76 -1.4 973,153 + amt1 Synpcc7942_0965 0.79 -0.3 973,161 - amt1 Synpcc7942_0965 0.80 +2.8 973,232 + amt1 Synpcc7942_0965 0.88 -1.7 973,240 - amt1 Synpcc7942_0965 0.88 +0.1 973,306 - +1.5 973,312 + -1.1 973,320 + +2.2 973,324 + -1.7 973,345 + -2.6 973,352 - -0.7 973,352 - +3.1 973,357 + -2.5 973,357 + +5.3 973,357 + -2.1 973,357 + +1.0 973,357 + -1.3 973,357 + -0.5 973,362 + -0.5 973,365 - -1.9 973,380 - -1.4 973,382 + -2.8 973,385 - +2.3 973,388 - -0.7 973,401 - -0.1 973,401 - +0.0 973,406 - -1.9 973,493 + Synpcc7942_0966 0.23 +1.6 973,524 - Synpcc7942_0966 0.28 +2.8 973,524 - Synpcc7942_0966 0.28 -3.2 973,551 - Synpcc7942_0966 0.33 +1.4 973,573 + Synpcc7942_0966 0.36 -0.5 973,575 - Synpcc7942_0966 0.37 +0.1 973,592 + Synpcc7942_0966 0.40 +1.1 973,592 + Synpcc7942_0966 0.40 +0.7 973,592 + Synpcc7942_0966 0.40 +1.6 973,592 + Synpcc7942_0966 0.40 -0.7 973,592 + Synpcc7942_0966 0.40 +1.2 973,592 + Synpcc7942_0966 0.40 +4.9 973,594 + Synpcc7942_0966 0.40 +0.3 973,600 - Synpcc7942_0966 0.41 -2.3 973,600 - Synpcc7942_0966 0.41 +1.4 973,602 + Synpcc7942_0966 0.41 -1.4 973,602 - Synpcc7942_0966 0.41 +3.4 973,607 + Synpcc7942_0966 0.42 -0.8 973,610 - Synpcc7942_0966 0.43 +5.0 973,612 + Synpcc7942_0966 0.43 -1.7 973,620 - Synpcc7942_0966 0.44 -1.2 973,630 - Synpcc7942_0966 0.46 -0.8 973,646 - Synpcc7942_0966 0.49 +0.8 973,653 + Synpcc7942_0966 0.50 +0.8 973,661 - Synpcc7942_0966 0.51 +0.0 973,695 + Synpcc7942_0966 0.57 +3.2 973,704 - Synpcc7942_0966 0.59 -1.0 973,706 + Synpcc7942_0966 0.59 +2.8 973,706 + Synpcc7942_0966 0.59 +0.5 973,714 - Synpcc7942_0966 0.60 +4.7 973,714 - Synpcc7942_0966 0.60 +1.0 973,714 - Synpcc7942_0966 0.60 -0.1 973,714 - Synpcc7942_0966 0.60 +2.9 973,716 + Synpcc7942_0966 0.61 +0.8 973,716 - Synpcc7942_0966 0.61 -0.8 973,716 - Synpcc7942_0966 0.61 +2.4 973,716 - Synpcc7942_0966 0.61 +1.7 973,720 + Synpcc7942_0966 0.61 -1.2 973,724 - Synpcc7942_0966 0.62 +1.9 973,728 - Synpcc7942_0966 0.63 +4.7 973,811 + Synpcc7942_0966 0.77 +0.5 973,817 - Synpcc7942_0966 0.78 +3.0 973,824 - Synpcc7942_0966 0.79 -0.9 973,824 - Synpcc7942_0966 0.79 +2.7 973,908 - +2.1 973,932 - -1.9 973,932 - -0.7 973,932 - +1.6 973,932 - -2.2 973,959 + +1.0 973,959 + -1.7 973,959 + -2.0 973,959 + +3.5 973,964 + -1.6 973,964 + -0.2 973,967 - +1.7 973,967 - -2.7 973,967 - +1.2 973,971 + +1.0 973,971 + -2.1 973,972 + +3.9 973,972 - -0.3 973,979 - -0.9 973,987 + -1.5 973,996 - -1.7 973,996 - -1.3 974,000 - +0.2 974,000 - -1.5 974,097 - -0.2
Or see this region's nucleotide sequence