Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0919 and psaK1 are separated by 196 nucleotides psaK1 and Synpcc7942_0921 are separated by 117 nucleotides Synpcc7942_0921 and gltX overlap by 4 nucleotides gltX and mtaP overlap by 1 nucleotides mtaP and nblS are separated by 51 nucleotides
Synpcc7942_0919: Synpcc7942_0919 - hypothetical protein, at 926,077 to 926,523
_0919
Synpcc7942_0920: psaK1 - photosystem I reaction center, at 926,720 to 926,974
psaK1
Synpcc7942_0921: Synpcc7942_0921 - hypothetical protein, at 927,092 to 927,394
_0921
Synpcc7942_0922: gltX - Glutamate--tRNA ligase, at 927,391 to 928,293
gltX
Synpcc7942_0923: mtaP - 5'-methylthioadenosine phosphorylase, at 928,293 to 929,168
mtaP
Synpcc7942_0924: nblS - multi-sensor signal transduction histidine kinase, at 929,220 to 931,214
nblS
Position (kb) 927
928
929 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 926.436 kb on + strand, within Synpcc7942_0919 at 926.500 kb on - strand at 926.526 kb on - strand at 926.672 kb on + strand at 926.699 kb on + strand at 926.704 kb on + strand at 926.709 kb on + strand at 926.801 kb on + strand, within psaK1 at 926.806 kb on + strand, within psaK1 at 926.895 kb on + strand, within psaK1 at 926.933 kb on + strand, within psaK1 at 926.935 kb on + strand, within psaK1 at 927.007 kb on - strand at 927.048 kb on - strand at 927.114 kb on + strand at 927.122 kb on - strand at 927.122 kb on - strand at 927.165 kb on - strand, within Synpcc7942_0921 at 927.177 kb on - strand, within Synpcc7942_0921 at 927.177 kb on - strand, within Synpcc7942_0921 at 927.181 kb on + strand, within Synpcc7942_0921 at 927.201 kb on - strand, within Synpcc7942_0921 at 927.202 kb on + strand, within Synpcc7942_0921 at 927.275 kb on + strand, within Synpcc7942_0921 at 927.275 kb on + strand, within Synpcc7942_0921 at 927.339 kb on - strand, within Synpcc7942_0921 at 927.368 kb on - strand at 927.389 kb on - strand at 927.420 kb on + strand at 927.420 kb on + strand at 927.420 kb on + strand at 927.428 kb on + strand at 927.446 kb on + strand at 927.451 kb on + strand at 927.454 kb on - strand at 927.478 kb on + strand at 927.486 kb on - strand, within gltX at 927.508 kb on - strand, within gltX at 927.526 kb on + strand, within gltX at 927.526 kb on + strand, within gltX at 927.532 kb on + strand, within gltX at 927.540 kb on - strand, within gltX at 927.586 kb on + strand, within gltX at 927.591 kb on + strand, within gltX at 927.605 kb on + strand, within gltX at 927.605 kb on + strand, within gltX at 927.613 kb on - strand, within gltX at 927.617 kb on + strand, within gltX at 927.669 kb on + strand, within gltX at 927.670 kb on - strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand at 927.672 kb on + strand, within gltX at 927.674 kb on + strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.695 kb on + strand, within gltX at 927.695 kb on - strand, within gltX at 927.695 kb on - strand, within gltX at 927.716 kb on - strand, within gltX at 927.738 kb on - strand, within gltX at 927.738 kb on - strand, within gltX at 927.801 kb on + strand, within gltX at 927.834 kb on - strand, within gltX at 927.834 kb on - strand, within gltX at 927.903 kb on + strand, within gltX at 927.911 kb on - strand, within gltX at 927.939 kb on - strand, within gltX at 927.940 kb on + strand, within gltX at 927.973 kb on - strand, within gltX at 928.003 kb on + strand, within gltX at 928.003 kb on + strand, within gltX at 928.026 kb on + strand, within gltX at 928.026 kb on + strand, within gltX at 928.027 kb on + strand, within gltX at 928.035 kb on - strand, within gltX at 928.035 kb on - strand, within gltX at 928.037 kb on + strand, within gltX at 928.045 kb on - strand, within gltX at 928.060 kb on - strand, within gltX at 928.062 kb on + strand, within gltX at 928.062 kb on + strand, within gltX at 928.067 kb on + strand, within gltX at 928.067 kb on + strand, within gltX at 928.067 kb on + strand, within gltX at 928.077 kb on + strand, within gltX at 928.077 kb on + strand, within gltX at 928.082 kb on + strand, within gltX at 928.090 kb on - strand, within gltX at 928.090 kb on - strand, within gltX at 928.092 kb on + strand, within gltX at 928.092 kb on + strand, within gltX at 928.092 kb on + strand, within gltX at 928.100 kb on - strand, within gltX at 928.120 kb on - strand, within gltX at 928.133 kb on - strand, within gltX at 928.151 kb on - strand, within gltX at 928.152 kb on + strand, within gltX at 928.184 kb on - strand, within gltX at 928.198 kb on + strand, within gltX at 928.354 kb on - strand at 928.365 kb on + strand at 928.372 kb on + strand at 928.372 kb on + strand at 928.408 kb on - strand, within mtaP at 928.506 kb on + strand, within mtaP at 928.514 kb on - strand, within mtaP at 928.535 kb on - strand, within mtaP at 928.535 kb on - strand, within mtaP at 928.544 kb on + strand, within mtaP at 928.635 kb on + strand, within mtaP at 928.635 kb on + strand, within mtaP at 928.640 kb on + strand, within mtaP at 928.643 kb on - strand, within mtaP at 928.664 kb on + strand, within mtaP at 928.683 kb on + strand, within mtaP at 928.690 kb on + strand, within mtaP at 928.755 kb on + strand, within mtaP at 928.755 kb on + strand, within mtaP at 928.758 kb on - strand, within mtaP at 928.760 kb on + strand, within mtaP at 928.762 kb on + strand, within mtaP at 928.765 kb on + strand, within mtaP at 928.765 kb on + strand, within mtaP at 928.765 kb on + strand, within mtaP at 928.765 kb on + strand, within mtaP at 928.768 kb on - strand, within mtaP at 928.768 kb on - strand, within mtaP at 928.768 kb on - strand, within mtaP at 928.768 kb on - strand, within mtaP at 928.768 kb on - strand, within mtaP at 928.773 kb on - strand, within mtaP at 928.825 kb on - strand, within mtaP at 928.866 kb on - strand, within mtaP at 928.878 kb on + strand, within mtaP at 928.892 kb on + strand, within mtaP at 928.899 kb on + strand, within mtaP at 928.909 kb on - strand, within mtaP at 929.032 kb on + strand, within mtaP at 929.032 kb on + strand, within mtaP at 929.104 kb on + strand at 929.104 kb on + strand at 929.112 kb on - strand at 929.127 kb on - strand at 929.137 kb on + strand at 929.172 kb on + strand at 929.201 kb on - strand at 929.260 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 926,436 + Synpcc7942_0919 0.80 -3.2 926,500 - -0.9 926,526 - +3.5 926,672 + +1.3 926,699 + -0.1 926,704 + -0.7 926,709 + -2.1 926,801 + psaK1 Synpcc7942_0920 0.32 -4.1 926,806 + psaK1 Synpcc7942_0920 0.34 -0.4 926,895 + psaK1 Synpcc7942_0920 0.69 -2.2 926,933 + psaK1 Synpcc7942_0920 0.84 -2.4 926,935 + psaK1 Synpcc7942_0920 0.84 -2.5 927,007 - -3.4 927,048 - -2.6 927,114 + -0.2 927,122 - +2.4 927,122 - +0.5 927,165 - Synpcc7942_0921 0.24 -2.1 927,177 - Synpcc7942_0921 0.28 +2.4 927,177 - Synpcc7942_0921 0.28 +2.5 927,181 + Synpcc7942_0921 0.29 -1.0 927,201 - Synpcc7942_0921 0.36 +2.5 927,202 + Synpcc7942_0921 0.36 +2.6 927,275 + Synpcc7942_0921 0.60 -0.6 927,275 + Synpcc7942_0921 0.60 +0.8 927,339 - Synpcc7942_0921 0.82 -2.3 927,368 - -0.6 927,389 - -0.2 927,420 + +0.5 927,420 + +2.7 927,420 + -2.0 927,428 + +2.0 927,446 + -2.0 927,451 + -2.7 927,454 - -1.3 927,478 + +2.4 927,486 - gltX Synpcc7942_0922 0.11 +2.3 927,508 - gltX Synpcc7942_0922 0.13 +2.4 927,526 + gltX Synpcc7942_0922 0.15 +0.0 927,526 + gltX Synpcc7942_0922 0.15 +1.0 927,532 + gltX Synpcc7942_0922 0.16 -1.4 927,540 - gltX Synpcc7942_0922 0.17 +3.6 927,586 + gltX Synpcc7942_0922 0.22 -1.0 927,591 + gltX Synpcc7942_0922 0.22 +1.0 927,605 + gltX Synpcc7942_0922 0.24 +1.2 927,605 + gltX Synpcc7942_0922 0.24 +1.6 927,613 - gltX Synpcc7942_0922 0.25 -1.4 927,617 + gltX Synpcc7942_0922 0.25 +3.2 927,669 + gltX Synpcc7942_0922 0.31 -2.4 927,670 - gltX Synpcc7942_0922 0.31 -1.1 927,672 + gltX Synpcc7942_0922 0.31 -2.2 927,672 + gltX Synpcc7942_0922 0.31 +0.7 927,672 + gltX Synpcc7942_0922 0.31 +3.7 927,672 + gltX Synpcc7942_0922 0.31 +1.8 927,672 + gltX Synpcc7942_0922 0.31 -0.8 927,672 + gltX Synpcc7942_0922 0.31 +4.7 927,672 + gltX Synpcc7942_0922 0.31 +1.2 927,672 + gltX Synpcc7942_0922 0.31 +1.7 927,672 + +1.0 927,672 + gltX Synpcc7942_0922 0.31 -0.2 927,674 + gltX Synpcc7942_0922 0.31 +2.7 927,680 - gltX Synpcc7942_0922 0.32 -0.3 927,680 - gltX Synpcc7942_0922 0.32 -1.2 927,680 - gltX Synpcc7942_0922 0.32 -2.7 927,680 - gltX Synpcc7942_0922 0.32 -1.6 927,680 - gltX Synpcc7942_0922 0.32 -0.3 927,680 - gltX Synpcc7942_0922 0.32 -1.9 927,680 - gltX Synpcc7942_0922 0.32 -1.0 927,680 - gltX Synpcc7942_0922 0.32 -0.8 927,680 - gltX Synpcc7942_0922 0.32 +2.0 927,680 - gltX Synpcc7942_0922 0.32 +0.7 927,695 + gltX Synpcc7942_0922 0.34 +2.8 927,695 - gltX Synpcc7942_0922 0.34 +0.7 927,695 - gltX Synpcc7942_0922 0.34 -0.3 927,716 - gltX Synpcc7942_0922 0.36 -2.7 927,738 - gltX Synpcc7942_0922 0.38 +0.6 927,738 - gltX Synpcc7942_0922 0.38 +1.2 927,801 + gltX Synpcc7942_0922 0.45 -1.6 927,834 - gltX Synpcc7942_0922 0.49 -2.5 927,834 - gltX Synpcc7942_0922 0.49 -2.7 927,903 + gltX Synpcc7942_0922 0.57 -0.4 927,911 - gltX Synpcc7942_0922 0.58 -1.6 927,939 - gltX Synpcc7942_0922 0.61 -1.7 927,940 + gltX Synpcc7942_0922 0.61 +0.8 927,973 - gltX Synpcc7942_0922 0.64 +0.6 928,003 + gltX Synpcc7942_0922 0.68 +0.2 928,003 + gltX Synpcc7942_0922 0.68 +1.9 928,026 + gltX Synpcc7942_0922 0.70 -0.2 928,026 + gltX Synpcc7942_0922 0.70 +1.7 928,027 + gltX Synpcc7942_0922 0.70 -1.4 928,035 - gltX Synpcc7942_0922 0.71 +3.9 928,035 - gltX Synpcc7942_0922 0.71 +1.6 928,037 + gltX Synpcc7942_0922 0.72 -0.1 928,045 - gltX Synpcc7942_0922 0.72 +3.9 928,060 - gltX Synpcc7942_0922 0.74 -3.2 928,062 + gltX Synpcc7942_0922 0.74 -0.1 928,062 + gltX Synpcc7942_0922 0.74 +1.6 928,067 + gltX Synpcc7942_0922 0.75 +0.9 928,067 + gltX Synpcc7942_0922 0.75 +1.4 928,067 + gltX Synpcc7942_0922 0.75 -0.3 928,077 + gltX Synpcc7942_0922 0.76 +3.2 928,077 + gltX Synpcc7942_0922 0.76 -1.0 928,082 + gltX Synpcc7942_0922 0.77 +0.7 928,090 - gltX Synpcc7942_0922 0.77 -0.4 928,090 - gltX Synpcc7942_0922 0.77 +0.2 928,092 + gltX Synpcc7942_0922 0.78 +2.2 928,092 + gltX Synpcc7942_0922 0.78 -0.4 928,092 + gltX Synpcc7942_0922 0.78 +0.1 928,100 - gltX Synpcc7942_0922 0.79 -2.9 928,120 - gltX Synpcc7942_0922 0.81 +2.3 928,133 - gltX Synpcc7942_0922 0.82 +0.4 928,151 - gltX Synpcc7942_0922 0.84 -1.1 928,152 + gltX Synpcc7942_0922 0.84 -1.8 928,184 - gltX Synpcc7942_0922 0.88 -0.6 928,198 + gltX Synpcc7942_0922 0.89 -2.7 928,354 - -0.6 928,365 + -0.7 928,372 + -1.6 928,372 + +3.1 928,408 - mtaP Synpcc7942_0923 0.13 -0.3 928,506 + mtaP Synpcc7942_0923 0.24 -1.5 928,514 - mtaP Synpcc7942_0923 0.25 -2.2 928,535 - mtaP Synpcc7942_0923 0.28 +0.3 928,535 - mtaP Synpcc7942_0923 0.28 +0.8 928,544 + mtaP Synpcc7942_0923 0.29 -3.1 928,635 + mtaP Synpcc7942_0923 0.39 -3.6 928,635 + mtaP Synpcc7942_0923 0.39 +0.4 928,640 + mtaP Synpcc7942_0923 0.40 -0.7 928,643 - mtaP Synpcc7942_0923 0.40 +1.8 928,664 + mtaP Synpcc7942_0923 0.42 +0.4 928,683 + mtaP Synpcc7942_0923 0.45 -1.3 928,690 + mtaP Synpcc7942_0923 0.45 -2.0 928,755 + mtaP Synpcc7942_0923 0.53 +2.4 928,755 + mtaP Synpcc7942_0923 0.53 +1.1 928,758 - mtaP Synpcc7942_0923 0.53 +0.9 928,760 + mtaP Synpcc7942_0923 0.53 -2.0 928,762 + mtaP Synpcc7942_0923 0.54 +1.0 928,765 + mtaP Synpcc7942_0923 0.54 +0.5 928,765 + mtaP Synpcc7942_0923 0.54 +0.6 928,765 + mtaP Synpcc7942_0923 0.54 +1.9 928,765 + mtaP Synpcc7942_0923 0.54 +3.2 928,768 - mtaP Synpcc7942_0923 0.54 +0.2 928,768 - mtaP Synpcc7942_0923 0.54 -1.2 928,768 - mtaP Synpcc7942_0923 0.54 -1.7 928,768 - mtaP Synpcc7942_0923 0.54 -1.9 928,768 - mtaP Synpcc7942_0923 0.54 +0.6 928,773 - mtaP Synpcc7942_0923 0.55 -1.7 928,825 - mtaP Synpcc7942_0923 0.61 +3.8 928,866 - mtaP Synpcc7942_0923 0.65 +0.8 928,878 + mtaP Synpcc7942_0923 0.67 -2.6 928,892 + mtaP Synpcc7942_0923 0.68 +1.1 928,899 + mtaP Synpcc7942_0923 0.69 -2.3 928,909 - mtaP Synpcc7942_0923 0.70 +1.4 929,032 + mtaP Synpcc7942_0923 0.84 -2.8 929,032 + mtaP Synpcc7942_0923 0.84 +1.5 929,104 + +0.4 929,104 + +3.8 929,112 - -0.5 929,127 - -1.6 929,137 + -0.7 929,172 + -1.0 929,201 - -1.5 929,260 + +3.4
Or see this region's nucleotide sequence