Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aas and Synpcc7942_0919 are separated by 48 nucleotides Synpcc7942_0919 and psaK1 are separated by 196 nucleotides psaK1 and Synpcc7942_0921 are separated by 117 nucleotides Synpcc7942_0921 and gltX overlap by 4 nucleotides
Synpcc7942_0918: aas - long-chain-fatty-acid CoA ligase, at 924,079 to 926,028
aas
Synpcc7942_0919: Synpcc7942_0919 - hypothetical protein, at 926,077 to 926,523
_0919
Synpcc7942_0920: psaK1 - photosystem I reaction center, at 926,720 to 926,974
psaK1
Synpcc7942_0921: Synpcc7942_0921 - hypothetical protein, at 927,092 to 927,394
_0921
Synpcc7942_0922: gltX - Glutamate--tRNA ligase, at 927,391 to 928,293
gltX
Position (kb) 926
927 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 925.739 kb on + strand, within aas at 925.744 kb on + strand, within aas at 925.762 kb on - strand, within aas at 925.802 kb on - strand, within aas at 925.814 kb on + strand, within aas at 925.822 kb on - strand, within aas at 925.822 kb on - strand, within aas at 925.835 kb on + strand at 925.840 kb on + strand at 925.855 kb on - strand at 925.890 kb on + strand at 925.910 kb on + strand at 926.042 kb on + strand at 926.050 kb on - strand at 926.075 kb on + strand at 926.092 kb on + strand at 926.093 kb on - strand at 926.126 kb on - strand, within Synpcc7942_0919 at 926.186 kb on - strand, within Synpcc7942_0919 at 926.279 kb on - strand, within Synpcc7942_0919 at 926.436 kb on + strand, within Synpcc7942_0919 at 926.500 kb on - strand at 926.526 kb on - strand at 926.672 kb on + strand at 926.699 kb on + strand at 926.704 kb on + strand at 926.709 kb on + strand at 926.801 kb on + strand, within psaK1 at 926.806 kb on + strand, within psaK1 at 926.895 kb on + strand, within psaK1 at 926.933 kb on + strand, within psaK1 at 926.935 kb on + strand, within psaK1 at 927.007 kb on - strand at 927.048 kb on - strand at 927.114 kb on + strand at 927.122 kb on - strand at 927.122 kb on - strand at 927.165 kb on - strand, within Synpcc7942_0921 at 927.177 kb on - strand, within Synpcc7942_0921 at 927.177 kb on - strand, within Synpcc7942_0921 at 927.181 kb on + strand, within Synpcc7942_0921 at 927.201 kb on - strand, within Synpcc7942_0921 at 927.202 kb on + strand, within Synpcc7942_0921 at 927.275 kb on + strand, within Synpcc7942_0921 at 927.275 kb on + strand, within Synpcc7942_0921 at 927.339 kb on - strand, within Synpcc7942_0921 at 927.368 kb on - strand at 927.389 kb on - strand at 927.420 kb on + strand at 927.420 kb on + strand at 927.420 kb on + strand at 927.428 kb on + strand at 927.446 kb on + strand at 927.451 kb on + strand at 927.454 kb on - strand at 927.478 kb on + strand at 927.486 kb on - strand, within gltX at 927.508 kb on - strand, within gltX at 927.526 kb on + strand, within gltX at 927.526 kb on + strand, within gltX at 927.532 kb on + strand, within gltX at 927.540 kb on - strand, within gltX at 927.586 kb on + strand, within gltX at 927.591 kb on + strand, within gltX at 927.605 kb on + strand, within gltX at 927.605 kb on + strand, within gltX at 927.613 kb on - strand, within gltX at 927.617 kb on + strand, within gltX at 927.669 kb on + strand, within gltX at 927.670 kb on - strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand, within gltX at 927.672 kb on + strand at 927.672 kb on + strand, within gltX at 927.674 kb on + strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.680 kb on - strand, within gltX at 927.695 kb on + strand, within gltX at 927.695 kb on - strand, within gltX at 927.695 kb on - strand, within gltX at 927.716 kb on - strand, within gltX at 927.738 kb on - strand, within gltX at 927.738 kb on - strand, within gltX at 927.801 kb on + strand, within gltX at 927.834 kb on - strand, within gltX at 927.834 kb on - strand, within gltX at 927.903 kb on + strand, within gltX at 927.911 kb on - strand, within gltX at 927.939 kb on - strand, within gltX at 927.940 kb on + strand, within gltX at 927.973 kb on - strand, within gltX
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 925,739 + aas Synpcc7942_0918 0.85 +0.4 925,744 + aas Synpcc7942_0918 0.85 +1.8 925,762 - aas Synpcc7942_0918 0.86 -1.8 925,802 - aas Synpcc7942_0918 0.88 +0.4 925,814 + aas Synpcc7942_0918 0.89 -0.8 925,822 - aas Synpcc7942_0918 0.89 -1.3 925,822 - aas Synpcc7942_0918 0.89 -0.8 925,835 + -1.8 925,840 + +2.4 925,855 - +0.1 925,890 + -1.4 925,910 + -0.8 926,042 + +6.6 926,050 - -0.9 926,075 + -2.4 926,092 + -2.2 926,093 - -4.3 926,126 - Synpcc7942_0919 0.11 -2.5 926,186 - Synpcc7942_0919 0.24 -2.8 926,279 - Synpcc7942_0919 0.45 -0.2 926,436 + Synpcc7942_0919 0.80 -3.2 926,500 - -0.9 926,526 - +3.5 926,672 + +1.3 926,699 + -0.1 926,704 + -0.7 926,709 + -2.1 926,801 + psaK1 Synpcc7942_0920 0.32 -4.1 926,806 + psaK1 Synpcc7942_0920 0.34 -0.4 926,895 + psaK1 Synpcc7942_0920 0.69 -2.2 926,933 + psaK1 Synpcc7942_0920 0.84 -2.4 926,935 + psaK1 Synpcc7942_0920 0.84 -2.5 927,007 - -3.4 927,048 - -2.6 927,114 + -0.2 927,122 - +2.4 927,122 - +0.5 927,165 - Synpcc7942_0921 0.24 -2.1 927,177 - Synpcc7942_0921 0.28 +2.4 927,177 - Synpcc7942_0921 0.28 +2.5 927,181 + Synpcc7942_0921 0.29 -1.0 927,201 - Synpcc7942_0921 0.36 +2.5 927,202 + Synpcc7942_0921 0.36 +2.6 927,275 + Synpcc7942_0921 0.60 -0.6 927,275 + Synpcc7942_0921 0.60 +0.8 927,339 - Synpcc7942_0921 0.82 -2.3 927,368 - -0.6 927,389 - -0.2 927,420 + +0.5 927,420 + +2.7 927,420 + -2.0 927,428 + +2.0 927,446 + -2.0 927,451 + -2.7 927,454 - -1.3 927,478 + +2.4 927,486 - gltX Synpcc7942_0922 0.11 +2.3 927,508 - gltX Synpcc7942_0922 0.13 +2.4 927,526 + gltX Synpcc7942_0922 0.15 +0.0 927,526 + gltX Synpcc7942_0922 0.15 +1.0 927,532 + gltX Synpcc7942_0922 0.16 -1.4 927,540 - gltX Synpcc7942_0922 0.17 +3.6 927,586 + gltX Synpcc7942_0922 0.22 -1.0 927,591 + gltX Synpcc7942_0922 0.22 +1.0 927,605 + gltX Synpcc7942_0922 0.24 +1.2 927,605 + gltX Synpcc7942_0922 0.24 +1.6 927,613 - gltX Synpcc7942_0922 0.25 -1.4 927,617 + gltX Synpcc7942_0922 0.25 +3.2 927,669 + gltX Synpcc7942_0922 0.31 -2.4 927,670 - gltX Synpcc7942_0922 0.31 -1.1 927,672 + gltX Synpcc7942_0922 0.31 -2.2 927,672 + gltX Synpcc7942_0922 0.31 +0.7 927,672 + gltX Synpcc7942_0922 0.31 +3.7 927,672 + gltX Synpcc7942_0922 0.31 +1.8 927,672 + gltX Synpcc7942_0922 0.31 -0.8 927,672 + gltX Synpcc7942_0922 0.31 +4.7 927,672 + gltX Synpcc7942_0922 0.31 +1.2 927,672 + gltX Synpcc7942_0922 0.31 +1.7 927,672 + +1.0 927,672 + gltX Synpcc7942_0922 0.31 -0.2 927,674 + gltX Synpcc7942_0922 0.31 +2.7 927,680 - gltX Synpcc7942_0922 0.32 -0.3 927,680 - gltX Synpcc7942_0922 0.32 -1.2 927,680 - gltX Synpcc7942_0922 0.32 -2.7 927,680 - gltX Synpcc7942_0922 0.32 -1.6 927,680 - gltX Synpcc7942_0922 0.32 -0.3 927,680 - gltX Synpcc7942_0922 0.32 -1.9 927,680 - gltX Synpcc7942_0922 0.32 -1.0 927,680 - gltX Synpcc7942_0922 0.32 -0.8 927,680 - gltX Synpcc7942_0922 0.32 +2.0 927,680 - gltX Synpcc7942_0922 0.32 +0.7 927,695 + gltX Synpcc7942_0922 0.34 +2.8 927,695 - gltX Synpcc7942_0922 0.34 +0.7 927,695 - gltX Synpcc7942_0922 0.34 -0.3 927,716 - gltX Synpcc7942_0922 0.36 -2.7 927,738 - gltX Synpcc7942_0922 0.38 +0.6 927,738 - gltX Synpcc7942_0922 0.38 +1.2 927,801 + gltX Synpcc7942_0922 0.45 -1.6 927,834 - gltX Synpcc7942_0922 0.49 -2.5 927,834 - gltX Synpcc7942_0922 0.49 -2.7 927,903 + gltX Synpcc7942_0922 0.57 -0.4 927,911 - gltX Synpcc7942_0922 0.58 -1.6 927,939 - gltX Synpcc7942_0922 0.61 -1.7 927,940 + gltX Synpcc7942_0922 0.61 +0.8 927,973 - gltX Synpcc7942_0922 0.64 +0.6
Or see this region's nucleotide sequence