Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glsF and Synpcc7942_0891 are separated by 377 nucleotides Synpcc7942_0891 and cofG are separated by 73 nucleotides cofG and psbAIII are separated by 81 nucleotides
Synpcc7942_0890: glsF - glutamate synthase (ferredoxin), at 896,748 to 901,358
glsF
Synpcc7942_0891: Synpcc7942_0891 - hypothetical protein, at 901,736 to 902,035
_0891
Synpcc7942_0892: cofG - FO synthase subunit 1, at 902,109 to 903,119
cofG
Synpcc7942_0893: psbAIII - photosystem q(b) protein, at 903,201 to 904,283
psbAIII
Position (kb) 902
903
904 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 901.309 kb on + strand at 901.329 kb on - strand at 901.329 kb on - strand at 901.353 kb on + strand at 901.353 kb on + strand at 901.361 kb on - strand at 901.387 kb on - strand at 901.410 kb on + strand at 901.433 kb on + strand at 901.441 kb on - strand at 901.443 kb on + strand at 901.467 kb on + strand at 901.492 kb on + strand at 901.642 kb on - strand at 901.651 kb on - strand at 901.671 kb on - strand at 901.687 kb on + strand at 901.776 kb on + strand, within Synpcc7942_0891 at 901.776 kb on + strand, within Synpcc7942_0891 at 901.776 kb on + strand, within Synpcc7942_0891 at 901.781 kb on + strand, within Synpcc7942_0891 at 901.786 kb on + strand, within Synpcc7942_0891 at 901.907 kb on - strand, within Synpcc7942_0891 at 901.973 kb on + strand, within Synpcc7942_0891 at 901.991 kb on + strand, within Synpcc7942_0891 at 901.991 kb on + strand, within Synpcc7942_0891 at 901.999 kb on - strand, within Synpcc7942_0891 at 902.015 kb on - strand at 902.024 kb on - strand at 902.037 kb on - strand at 902.037 kb on - strand at 902.064 kb on + strand at 902.078 kb on + strand at 902.116 kb on + strand at 902.116 kb on + strand at 902.124 kb on - strand at 902.137 kb on - strand at 902.144 kb on + strand at 902.182 kb on - strand at 902.192 kb on + strand at 902.200 kb on - strand at 902.223 kb on + strand, within cofG at 902.229 kb on + strand, within cofG at 902.248 kb on + strand, within cofG at 902.298 kb on + strand, within cofG at 902.303 kb on + strand, within cofG at 902.311 kb on - strand, within cofG at 902.311 kb on - strand, within cofG at 902.333 kb on + strand, within cofG at 902.341 kb on - strand, within cofG at 902.343 kb on - strand, within cofG at 902.380 kb on - strand, within cofG at 902.403 kb on + strand, within cofG at 902.411 kb on - strand, within cofG at 902.463 kb on + strand, within cofG at 902.463 kb on + strand, within cofG at 902.473 kb on - strand, within cofG at 902.502 kb on + strand, within cofG at 902.550 kb on - strand, within cofG at 902.561 kb on + strand, within cofG at 902.589 kb on - strand, within cofG at 902.592 kb on + strand, within cofG at 902.611 kb on - strand, within cofG at 902.618 kb on - strand, within cofG at 902.629 kb on + strand, within cofG at 902.629 kb on + strand, within cofG at 902.639 kb on + strand, within cofG at 902.639 kb on + strand, within cofG at 902.647 kb on - strand, within cofG at 902.647 kb on - strand, within cofG at 902.647 kb on - strand, within cofG at 902.676 kb on - strand, within cofG at 902.679 kb on - strand, within cofG at 902.686 kb on - strand, within cofG at 902.688 kb on + strand, within cofG at 902.715 kb on + strand, within cofG at 902.734 kb on + strand, within cofG at 902.739 kb on - strand, within cofG at 902.750 kb on - strand, within cofG at 902.776 kb on - strand, within cofG at 902.778 kb on + strand, within cofG at 902.778 kb on + strand, within cofG at 902.786 kb on - strand, within cofG at 902.786 kb on - strand, within cofG at 902.791 kb on + strand at 902.791 kb on + strand, within cofG at 902.799 kb on - strand, within cofG at 902.817 kb on + strand, within cofG at 902.825 kb on - strand, within cofG at 902.825 kb on - strand, within cofG at 902.861 kb on - strand, within cofG at 902.933 kb on - strand, within cofG at 902.964 kb on + strand, within cofG at 902.965 kb on - strand, within cofG at 902.972 kb on - strand, within cofG at 903.010 kb on + strand, within cofG at 903.035 kb on - strand at 903.053 kb on - strand at 903.054 kb on - strand at 903.074 kb on - strand at 903.078 kb on + strand at 903.078 kb on + strand at 903.229 kb on - strand at 903.311 kb on - strand, within psbAIII
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 901,309 + -0.3 901,329 - -1.0 901,329 - +0.4 901,353 + +0.8 901,353 + +0.8 901,361 - +1.5 901,387 - +2.5 901,410 + +1.4 901,433 + +0.3 901,441 - -1.5 901,443 + +1.7 901,467 + +2.8 901,492 + +2.0 901,642 - +4.5 901,651 - -1.3 901,671 - -1.9 901,687 + -0.7 901,776 + Synpcc7942_0891 0.13 +1.5 901,776 + Synpcc7942_0891 0.13 +2.5 901,776 + Synpcc7942_0891 0.13 +2.6 901,781 + Synpcc7942_0891 0.15 -1.6 901,786 + Synpcc7942_0891 0.17 -0.2 901,907 - Synpcc7942_0891 0.57 +2.4 901,973 + Synpcc7942_0891 0.79 +1.7 901,991 + Synpcc7942_0891 0.85 -4.5 901,991 + Synpcc7942_0891 0.85 -0.6 901,999 - Synpcc7942_0891 0.88 +0.2 902,015 - +1.1 902,024 - -0.2 902,037 - -1.6 902,037 - +1.0 902,064 + -0.3 902,078 + +1.5 902,116 + +1.2 902,116 + +2.1 902,124 - -1.6 902,137 - -0.8 902,144 + +1.5 902,182 - +4.0 902,192 + +4.0 902,200 - +5.8 902,223 + cofG Synpcc7942_0892 0.11 +4.5 902,229 + cofG Synpcc7942_0892 0.12 +2.0 902,248 + cofG Synpcc7942_0892 0.14 +4.3 902,298 + cofG Synpcc7942_0892 0.19 +0.7 902,303 + cofG Synpcc7942_0892 0.19 +2.1 902,311 - cofG Synpcc7942_0892 0.20 +3.5 902,311 - cofG Synpcc7942_0892 0.20 +3.3 902,333 + cofG Synpcc7942_0892 0.22 +0.8 902,341 - cofG Synpcc7942_0892 0.23 +3.3 902,343 - cofG Synpcc7942_0892 0.23 +0.7 902,380 - cofG Synpcc7942_0892 0.27 +5.5 902,403 + cofG Synpcc7942_0892 0.29 +1.6 902,411 - cofG Synpcc7942_0892 0.30 +5.7 902,463 + cofG Synpcc7942_0892 0.35 +4.9 902,463 + cofG Synpcc7942_0892 0.35 +4.0 902,473 - cofG Synpcc7942_0892 0.36 +2.9 902,502 + cofG Synpcc7942_0892 0.39 +2.2 902,550 - cofG Synpcc7942_0892 0.44 +0.2 902,561 + cofG Synpcc7942_0892 0.45 +2.7 902,589 - cofG Synpcc7942_0892 0.47 +2.8 902,592 + cofG Synpcc7942_0892 0.48 +4.5 902,611 - cofG Synpcc7942_0892 0.50 +4.3 902,618 - cofG Synpcc7942_0892 0.50 +1.2 902,629 + cofG Synpcc7942_0892 0.51 +1.1 902,629 + cofG Synpcc7942_0892 0.51 +3.0 902,639 + cofG Synpcc7942_0892 0.52 +3.7 902,639 + cofG Synpcc7942_0892 0.52 +4.3 902,647 - cofG Synpcc7942_0892 0.53 +4.6 902,647 - cofG Synpcc7942_0892 0.53 +4.2 902,647 - cofG Synpcc7942_0892 0.53 +2.4 902,676 - cofG Synpcc7942_0892 0.56 +5.8 902,679 - cofG Synpcc7942_0892 0.56 +5.4 902,686 - cofG Synpcc7942_0892 0.57 +3.6 902,688 + cofG Synpcc7942_0892 0.57 +5.0 902,715 + cofG Synpcc7942_0892 0.60 +1.7 902,734 + cofG Synpcc7942_0892 0.62 +4.7 902,739 - cofG Synpcc7942_0892 0.62 +4.0 902,750 - cofG Synpcc7942_0892 0.63 +2.8 902,776 - cofG Synpcc7942_0892 0.66 +0.4 902,778 + cofG Synpcc7942_0892 0.66 +4.6 902,778 + cofG Synpcc7942_0892 0.66 +6.2 902,786 - cofG Synpcc7942_0892 0.67 +6.4 902,786 - cofG Synpcc7942_0892 0.67 +3.8 902,791 + +5.0 902,791 + cofG Synpcc7942_0892 0.67 +3.2 902,799 - cofG Synpcc7942_0892 0.68 +2.6 902,817 + cofG Synpcc7942_0892 0.70 +4.4 902,825 - cofG Synpcc7942_0892 0.71 +3.7 902,825 - cofG Synpcc7942_0892 0.71 +3.7 902,861 - cofG Synpcc7942_0892 0.74 +1.1 902,933 - cofG Synpcc7942_0892 0.82 +3.7 902,964 + cofG Synpcc7942_0892 0.85 +4.0 902,965 - cofG Synpcc7942_0892 0.85 +3.0 902,972 - cofG Synpcc7942_0892 0.85 +4.4 903,010 + cofG Synpcc7942_0892 0.89 +3.7 903,035 - +8.4 903,053 - +2.9 903,054 - +5.6 903,074 - +0.7 903,078 + +1.3 903,078 + -0.5 903,229 - +0.9 903,311 - psbAIII Synpcc7942_0893 0.10 +0.4
Or see this region's nucleotide sequence