Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rne and rhnB overlap by 17 nucleotides rhnB and Synpcc7942_0880 are separated by 22 nucleotides Synpcc7942_0880 and pheA are separated by 79 nucleotides pheA and lon are separated by 12 nucleotides
Synpcc7942_0878: rne - ribonuclease, Rne/Rng family, at 883,835 to 886,261
rne
Synpcc7942_0879: rhnB - RNase HII, at 886,245 to 886,892
rhnB
Synpcc7942_0880: Synpcc7942_0880 - hypothetical protein, at 886,915 to 887,472
_0880
Synpcc7942_0881: pheA - prephenate dehydratase, at 887,552 to 888,427
pheA
Synpcc7942_0882: lon - Peptidase S16, lon-like, at 888,440 to 889,096
lon
Position (kb) 886
887
888 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 885.962 kb on + strand, within rne at 885.962 kb on + strand, within rne at 885.967 kb on + strand, within rne at 885.986 kb on - strand, within rne at 885.987 kb on + strand, within rne at 885.987 kb on + strand, within rne at 885.997 kb on + strand, within rne at 885.997 kb on + strand, within rne at 885.997 kb on + strand, within rne at 886.005 kb on - strand at 886.005 kb on - strand, within rne at 886.048 kb on - strand at 886.056 kb on + strand at 886.064 kb on - strand at 886.123 kb on + strand at 886.131 kb on - strand at 886.170 kb on - strand at 886.187 kb on + strand at 886.268 kb on - strand at 886.316 kb on + strand, within rhnB at 886.459 kb on - strand, within rhnB at 886.465 kb on + strand, within rhnB at 886.481 kb on + strand, within rhnB at 886.488 kb on + strand, within rhnB at 886.488 kb on + strand, within rhnB at 886.488 kb on + strand, within rhnB at 886.488 kb on + strand, within rhnB at 886.493 kb on + strand, within rhnB at 886.494 kb on + strand, within rhnB at 886.495 kb on - strand, within rhnB at 886.496 kb on - strand, within rhnB at 886.496 kb on - strand, within rhnB at 886.576 kb on - strand, within rhnB at 886.591 kb on - strand, within rhnB at 886.591 kb on - strand, within rhnB at 886.660 kb on - strand, within rhnB at 886.714 kb on - strand, within rhnB at 886.714 kb on - strand, within rhnB at 886.777 kb on - strand, within rhnB at 886.788 kb on + strand, within rhnB at 886.788 kb on + strand, within rhnB at 886.788 kb on + strand, within rhnB at 886.794 kb on - strand, within rhnB at 886.796 kb on - strand, within rhnB at 886.835 kb on - strand at 886.838 kb on + strand at 886.881 kb on + strand at 886.886 kb on + strand at 886.886 kb on + strand at 886.889 kb on - strand at 886.889 kb on - strand at 886.946 kb on + strand at 887.024 kb on + strand, within Synpcc7942_0880 at 887.024 kb on + strand, within Synpcc7942_0880 at 887.024 kb on + strand, within Synpcc7942_0880 at 887.032 kb on - strand at 887.032 kb on - strand, within Synpcc7942_0880 at 887.032 kb on - strand, within Synpcc7942_0880 at 887.042 kb on - strand, within Synpcc7942_0880 at 887.048 kb on + strand, within Synpcc7942_0880 at 887.090 kb on + strand, within Synpcc7942_0880 at 887.117 kb on + strand, within Synpcc7942_0880 at 887.123 kb on + strand, within Synpcc7942_0880 at 887.123 kb on + strand, within Synpcc7942_0880 at 887.123 kb on + strand, within Synpcc7942_0880 at 887.123 kb on - strand, within Synpcc7942_0880 at 887.191 kb on - strand, within Synpcc7942_0880 at 887.262 kb on + strand, within Synpcc7942_0880 at 887.303 kb on - strand, within Synpcc7942_0880 at 887.332 kb on + strand, within Synpcc7942_0880 at 887.332 kb on + strand, within Synpcc7942_0880 at 887.354 kb on + strand, within Synpcc7942_0880 at 887.356 kb on + strand, within Synpcc7942_0880 at 887.356 kb on + strand, within Synpcc7942_0880 at 887.356 kb on + strand, within Synpcc7942_0880 at 887.362 kb on - strand, within Synpcc7942_0880 at 887.362 kb on - strand, within Synpcc7942_0880 at 887.393 kb on + strand, within Synpcc7942_0880 at 887.396 kb on - strand, within Synpcc7942_0880 at 887.406 kb on - strand, within Synpcc7942_0880 at 887.540 kb on - strand at 887.542 kb on + strand at 887.556 kb on - strand at 887.556 kb on - strand at 888.387 kb on + strand at 888.387 kb on + strand at 888.395 kb on - strand at 888.395 kb on - strand at 888.395 kb on - strand at 888.445 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 885,962 + rne Synpcc7942_0878 0.88 -2.0 885,962 + rne Synpcc7942_0878 0.88 -0.6 885,967 + rne Synpcc7942_0878 0.88 +1.7 885,986 - rne Synpcc7942_0878 0.89 +0.3 885,987 + rne Synpcc7942_0878 0.89 -0.6 885,987 + rne Synpcc7942_0878 0.89 +0.9 885,997 + rne Synpcc7942_0878 0.89 +1.3 885,997 + rne Synpcc7942_0878 0.89 +2.2 885,997 + rne Synpcc7942_0878 0.89 +0.1 886,005 - -0.3 886,005 - rne Synpcc7942_0878 0.89 -1.3 886,048 - -2.3 886,056 + +1.0 886,064 - -0.2 886,123 + -0.9 886,131 - -0.4 886,170 - -0.8 886,187 + -1.0 886,268 - +0.9 886,316 + rhnB Synpcc7942_0879 0.11 +2.2 886,459 - rhnB Synpcc7942_0879 0.33 -1.7 886,465 + rhnB Synpcc7942_0879 0.34 -1.6 886,481 + rhnB Synpcc7942_0879 0.36 +0.1 886,488 + rhnB Synpcc7942_0879 0.38 +0.8 886,488 + rhnB Synpcc7942_0879 0.38 +2.2 886,488 + rhnB Synpcc7942_0879 0.38 -0.3 886,488 + rhnB Synpcc7942_0879 0.38 +1.1 886,493 + rhnB Synpcc7942_0879 0.38 -1.4 886,494 + rhnB Synpcc7942_0879 0.38 +0.7 886,495 - rhnB Synpcc7942_0879 0.39 -1.4 886,496 - rhnB Synpcc7942_0879 0.39 +0.5 886,496 - rhnB Synpcc7942_0879 0.39 +3.6 886,576 - rhnB Synpcc7942_0879 0.51 -1.1 886,591 - rhnB Synpcc7942_0879 0.53 -0.9 886,591 - rhnB Synpcc7942_0879 0.53 -2.3 886,660 - rhnB Synpcc7942_0879 0.64 +0.7 886,714 - rhnB Synpcc7942_0879 0.72 -1.3 886,714 - rhnB Synpcc7942_0879 0.72 +0.3 886,777 - rhnB Synpcc7942_0879 0.82 -1.7 886,788 + rhnB Synpcc7942_0879 0.84 +0.3 886,788 + rhnB Synpcc7942_0879 0.84 -1.6 886,788 + rhnB Synpcc7942_0879 0.84 -2.4 886,794 - rhnB Synpcc7942_0879 0.85 +1.2 886,796 - rhnB Synpcc7942_0879 0.85 -2.1 886,835 - +0.4 886,838 + -1.5 886,881 + +0.2 886,886 + -1.6 886,886 + +4.5 886,889 - +0.6 886,889 - +2.3 886,946 + -3.4 887,024 + Synpcc7942_0880 0.20 -0.8 887,024 + Synpcc7942_0880 0.20 -3.0 887,024 + Synpcc7942_0880 0.20 -0.0 887,032 - -1.9 887,032 - Synpcc7942_0880 0.21 -1.8 887,032 - Synpcc7942_0880 0.21 -2.3 887,042 - Synpcc7942_0880 0.23 -2.5 887,048 + Synpcc7942_0880 0.24 -1.8 887,090 + Synpcc7942_0880 0.31 -2.8 887,117 + Synpcc7942_0880 0.36 -0.7 887,123 + Synpcc7942_0880 0.37 -1.5 887,123 + Synpcc7942_0880 0.37 -2.2 887,123 + Synpcc7942_0880 0.37 -2.5 887,123 - Synpcc7942_0880 0.37 -5.3 887,191 - Synpcc7942_0880 0.49 -3.0 887,262 + Synpcc7942_0880 0.62 -2.8 887,303 - Synpcc7942_0880 0.70 -2.1 887,332 + Synpcc7942_0880 0.75 -1.9 887,332 + Synpcc7942_0880 0.75 +0.8 887,354 + Synpcc7942_0880 0.79 -2.2 887,356 + Synpcc7942_0880 0.79 +1.0 887,356 + Synpcc7942_0880 0.79 -1.0 887,356 + Synpcc7942_0880 0.79 -4.4 887,362 - Synpcc7942_0880 0.80 -2.9 887,362 - Synpcc7942_0880 0.80 -1.7 887,393 + Synpcc7942_0880 0.86 -2.2 887,396 - Synpcc7942_0880 0.86 -3.4 887,406 - Synpcc7942_0880 0.88 -3.3 887,540 - -4.6 887,542 + -1.0 887,556 - -0.0 887,556 - +1.8 888,387 + -0.4 888,387 + +1.1 888,395 - +0.7 888,395 - +0.4 888,395 - +1.2 888,445 - +1.5
Or see this region's nucleotide sequence