Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0861 and Synpcc7942_0862 are separated by 126 nucleotides Synpcc7942_0862 and Synpcc7942_0863 are separated by 53 nucleotides Synpcc7942_0863 and Synpcc7942_0864 are separated by 186 nucleotides Synpcc7942_0864 and Synpcc7942_0865 are separated by 72 nucleotides
Synpcc7942_0861: Synpcc7942_0861 - bifunctional sterol desaturase/short chain dehydrogenase, at 864,180 to 865,469
_0861
Synpcc7942_0862: Synpcc7942_0862 - hypothetical protein, at 865,596 to 865,973
_0862
Synpcc7942_0863: Synpcc7942_0863 - hypothetical protein, at 866,027 to 867,208
_0863
Synpcc7942_0864: Synpcc7942_0864 - hypothetical protein, at 867,395 to 867,913
_0864
Synpcc7942_0865: Synpcc7942_0865 - nucleotide-binding protein, at 867,986 to 868,477
_0865
Position (kb) 866
867
868 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 865.461 kb on - strand at 865.528 kb on - strand at 865.549 kb on + strand at 865.557 kb on - strand at 865.558 kb on + strand at 865.562 kb on - strand at 865.576 kb on + strand at 865.576 kb on - strand at 865.576 kb on - strand at 865.582 kb on - strand at 865.583 kb on - strand at 865.587 kb on + strand at 865.608 kb on + strand at 865.610 kb on + strand at 865.613 kb on + strand at 865.621 kb on - strand at 865.621 kb on - strand at 865.625 kb on + strand at 865.625 kb on + strand at 865.635 kb on + strand, within Synpcc7942_0862 at 865.643 kb on + strand, within Synpcc7942_0862 at 865.643 kb on + strand, within Synpcc7942_0862 at 865.643 kb on + strand, within Synpcc7942_0862 at 865.643 kb on - strand, within Synpcc7942_0862 at 865.643 kb on - strand, within Synpcc7942_0862 at 865.651 kb on - strand, within Synpcc7942_0862 at 865.651 kb on - strand, within Synpcc7942_0862 at 865.651 kb on - strand, within Synpcc7942_0862 at 865.656 kb on - strand, within Synpcc7942_0862 at 865.660 kb on - strand, within Synpcc7942_0862 at 865.665 kb on - strand, within Synpcc7942_0862 at 865.681 kb on + strand, within Synpcc7942_0862 at 865.688 kb on - strand, within Synpcc7942_0862 at 865.694 kb on - strand, within Synpcc7942_0862 at 865.704 kb on + strand, within Synpcc7942_0862 at 865.712 kb on - strand, within Synpcc7942_0862 at 865.719 kb on + strand, within Synpcc7942_0862 at 865.724 kb on + strand, within Synpcc7942_0862 at 865.725 kb on - strand, within Synpcc7942_0862 at 865.727 kb on + strand, within Synpcc7942_0862 at 865.727 kb on - strand, within Synpcc7942_0862 at 865.727 kb on - strand, within Synpcc7942_0862 at 865.727 kb on - strand, within Synpcc7942_0862 at 865.737 kb on + strand, within Synpcc7942_0862 at 865.737 kb on + strand, within Synpcc7942_0862 at 865.745 kb on - strand, within Synpcc7942_0862 at 865.777 kb on - strand, within Synpcc7942_0862 at 865.797 kb on + strand, within Synpcc7942_0862 at 865.797 kb on + strand, within Synpcc7942_0862 at 865.805 kb on - strand, within Synpcc7942_0862 at 865.812 kb on + strand, within Synpcc7942_0862 at 865.820 kb on + strand, within Synpcc7942_0862 at 865.820 kb on + strand, within Synpcc7942_0862 at 865.823 kb on - strand, within Synpcc7942_0862 at 865.826 kb on - strand, within Synpcc7942_0862 at 865.828 kb on - strand, within Synpcc7942_0862 at 865.836 kb on + strand, within Synpcc7942_0862 at 865.844 kb on - strand, within Synpcc7942_0862 at 865.888 kb on - strand, within Synpcc7942_0862 at 865.903 kb on + strand, within Synpcc7942_0862 at 865.903 kb on - strand, within Synpcc7942_0862 at 865.972 kb on + strand at 865.982 kb on - strand at 866.035 kb on + strand at 866.035 kb on + strand at 866.038 kb on - strand at 866.045 kb on - strand at 866.047 kb on + strand at 866.047 kb on + strand at 866.053 kb on - strand at 866.094 kb on - strand at 866.112 kb on + strand at 866.126 kb on + strand at 866.147 kb on + strand, within Synpcc7942_0863 at 866.188 kb on + strand, within Synpcc7942_0863 at 866.221 kb on + strand, within Synpcc7942_0863 at 866.229 kb on - strand, within Synpcc7942_0863 at 866.236 kb on - strand, within Synpcc7942_0863 at 866.241 kb on + strand, within Synpcc7942_0863 at 866.249 kb on - strand, within Synpcc7942_0863 at 866.249 kb on - strand, within Synpcc7942_0863 at 866.249 kb on - strand, within Synpcc7942_0863 at 866.256 kb on + strand, within Synpcc7942_0863 at 866.283 kb on + strand, within Synpcc7942_0863 at 866.310 kb on + strand, within Synpcc7942_0863 at 866.332 kb on + strand, within Synpcc7942_0863 at 866.332 kb on + strand, within Synpcc7942_0863 at 866.387 kb on - strand, within Synpcc7942_0863 at 866.390 kb on + strand, within Synpcc7942_0863 at 866.397 kb on - strand, within Synpcc7942_0863 at 866.412 kb on + strand, within Synpcc7942_0863 at 866.423 kb on - strand, within Synpcc7942_0863 at 866.456 kb on - strand, within Synpcc7942_0863 at 866.458 kb on + strand, within Synpcc7942_0863 at 866.458 kb on + strand at 866.466 kb on - strand, within Synpcc7942_0863 at 866.474 kb on - strand, within Synpcc7942_0863 at 866.491 kb on + strand, within Synpcc7942_0863 at 866.507 kb on - strand, within Synpcc7942_0863 at 866.525 kb on + strand, within Synpcc7942_0863 at 866.525 kb on + strand, within Synpcc7942_0863 at 866.533 kb on - strand, within Synpcc7942_0863 at 866.533 kb on - strand, within Synpcc7942_0863 at 866.560 kb on + strand, within Synpcc7942_0863 at 866.583 kb on - strand, within Synpcc7942_0863 at 866.617 kb on + strand, within Synpcc7942_0863 at 866.617 kb on + strand, within Synpcc7942_0863 at 866.632 kb on + strand, within Synpcc7942_0863 at 866.632 kb on + strand, within Synpcc7942_0863 at 866.640 kb on - strand, within Synpcc7942_0863 at 866.640 kb on - strand at 866.644 kb on + strand, within Synpcc7942_0863 at 866.655 kb on + strand, within Synpcc7942_0863 at 866.663 kb on - strand, within Synpcc7942_0863 at 866.684 kb on - strand, within Synpcc7942_0863 at 866.733 kb on + strand, within Synpcc7942_0863 at 866.738 kb on + strand, within Synpcc7942_0863 at 866.738 kb on + strand, within Synpcc7942_0863 at 866.746 kb on - strand, within Synpcc7942_0863 at 866.757 kb on + strand at 866.765 kb on - strand, within Synpcc7942_0863 at 866.765 kb on - strand, within Synpcc7942_0863 at 866.776 kb on + strand, within Synpcc7942_0863 at 866.776 kb on + strand, within Synpcc7942_0863 at 866.776 kb on + strand, within Synpcc7942_0863 at 866.789 kb on - strand, within Synpcc7942_0863 at 866.789 kb on - strand, within Synpcc7942_0863 at 866.822 kb on + strand, within Synpcc7942_0863 at 866.822 kb on + strand, within Synpcc7942_0863 at 866.882 kb on + strand, within Synpcc7942_0863 at 866.890 kb on - strand, within Synpcc7942_0863 at 866.890 kb on - strand, within Synpcc7942_0863 at 866.890 kb on - strand, within Synpcc7942_0863 at 866.890 kb on - strand, within Synpcc7942_0863 at 866.890 kb on - strand, within Synpcc7942_0863 at 866.902 kb on + strand, within Synpcc7942_0863 at 867.014 kb on - strand, within Synpcc7942_0863 at 867.088 kb on - strand, within Synpcc7942_0863 at 867.109 kb on + strand at 867.129 kb on + strand at 867.146 kb on + strand at 867.156 kb on + strand at 867.188 kb on - strand at 867.221 kb on - strand at 867.221 kb on - strand at 867.239 kb on - strand at 867.301 kb on + strand at 867.301 kb on + strand at 867.301 kb on + strand at 867.301 kb on + strand at 867.301 kb on + strand at 867.301 kb on + strand at 867.301 kb on + strand at 867.301 kb on + strand at 867.301 kb on + strand at 867.301 kb on + strand at 867.301 kb on + strand at 867.304 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.309 kb on - strand at 867.311 kb on + strand at 867.311 kb on + strand at 867.314 kb on - strand at 867.314 kb on - strand at 867.319 kb on - strand at 867.329 kb on - strand at 867.329 kb on - strand at 867.332 kb on + strand at 867.332 kb on + strand at 867.332 kb on + strand at 867.339 kb on - strand at 867.347 kb on + strand at 867.347 kb on + strand at 867.347 kb on + strand at 867.403 kb on + strand at 867.415 kb on + strand at 867.421 kb on + strand at 867.429 kb on - strand at 867.481 kb on + strand, within Synpcc7942_0864 at 867.769 kb on + strand at 867.769 kb on + strand at 867.917 kb on + strand at 867.925 kb on - strand at 867.925 kb on - strand at 867.926 kb on + strand at 867.934 kb on - strand at 867.963 kb on - strand at 868.029 kb on + strand at 868.029 kb on + strand at 868.049 kb on + strand, within Synpcc7942_0865 at 868.068 kb on + strand, within Synpcc7942_0865 at 868.107 kb on + strand, within Synpcc7942_0865
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 865,461 - -0.2 865,528 - -1.9 865,549 + +0.3 865,557 - -1.9 865,558 + -0.7 865,562 - -1.0 865,576 + +0.5 865,576 - +0.9 865,576 - -1.9 865,582 - +1.7 865,583 - -2.2 865,587 + -1.3 865,608 + -1.5 865,610 + +3.5 865,613 + -0.5 865,621 - +1.4 865,621 - -1.5 865,625 + +3.5 865,625 + +3.2 865,635 + Synpcc7942_0862 0.10 -1.2 865,643 + Synpcc7942_0862 0.12 +1.5 865,643 + Synpcc7942_0862 0.12 +1.4 865,643 + Synpcc7942_0862 0.12 +3.3 865,643 - Synpcc7942_0862 0.12 +4.3 865,643 - Synpcc7942_0862 0.12 +1.9 865,651 - Synpcc7942_0862 0.15 +2.7 865,651 - Synpcc7942_0862 0.15 +1.7 865,651 - Synpcc7942_0862 0.15 -1.3 865,656 - Synpcc7942_0862 0.16 +4.7 865,660 - Synpcc7942_0862 0.17 +2.4 865,665 - Synpcc7942_0862 0.18 -2.4 865,681 + Synpcc7942_0862 0.22 -1.7 865,688 - Synpcc7942_0862 0.24 +3.3 865,694 - Synpcc7942_0862 0.26 +1.1 865,704 + Synpcc7942_0862 0.29 +4.2 865,712 - Synpcc7942_0862 0.31 +0.7 865,719 + Synpcc7942_0862 0.33 -0.4 865,724 + Synpcc7942_0862 0.34 -0.5 865,725 - Synpcc7942_0862 0.34 +2.0 865,727 + Synpcc7942_0862 0.35 -0.8 865,727 - Synpcc7942_0862 0.35 +3.2 865,727 - Synpcc7942_0862 0.35 -0.5 865,727 - Synpcc7942_0862 0.35 +3.1 865,737 + Synpcc7942_0862 0.37 +0.4 865,737 + Synpcc7942_0862 0.37 +2.3 865,745 - Synpcc7942_0862 0.39 +0.3 865,777 - Synpcc7942_0862 0.48 +1.1 865,797 + Synpcc7942_0862 0.53 -1.4 865,797 + Synpcc7942_0862 0.53 +0.3 865,805 - Synpcc7942_0862 0.55 +4.5 865,812 + Synpcc7942_0862 0.57 +4.7 865,820 + Synpcc7942_0862 0.59 +0.2 865,820 + Synpcc7942_0862 0.59 +4.6 865,823 - Synpcc7942_0862 0.60 +3.2 865,826 - Synpcc7942_0862 0.61 -3.3 865,828 - Synpcc7942_0862 0.61 -2.6 865,836 + Synpcc7942_0862 0.63 -0.6 865,844 - Synpcc7942_0862 0.66 +1.0 865,888 - Synpcc7942_0862 0.77 +1.5 865,903 + Synpcc7942_0862 0.81 +1.1 865,903 - Synpcc7942_0862 0.81 -0.3 865,972 + +0.8 865,982 - +0.5 866,035 + -0.2 866,035 + -1.1 866,038 - -2.7 866,045 - -0.8 866,047 + -1.3 866,047 + -1.5 866,053 - -2.3 866,094 - -3.2 866,112 + -1.6 866,126 + -1.7 866,147 + Synpcc7942_0863 0.10 -3.4 866,188 + Synpcc7942_0863 0.14 -5.0 866,221 + Synpcc7942_0863 0.16 -2.5 866,229 - Synpcc7942_0863 0.17 -4.5 866,236 - Synpcc7942_0863 0.18 -1.8 866,241 + Synpcc7942_0863 0.18 -1.2 866,249 - Synpcc7942_0863 0.19 -2.7 866,249 - Synpcc7942_0863 0.19 -2.0 866,249 - Synpcc7942_0863 0.19 -2.1 866,256 + Synpcc7942_0863 0.19 -2.8 866,283 + Synpcc7942_0863 0.22 -2.2 866,310 + Synpcc7942_0863 0.24 -5.0 866,332 + Synpcc7942_0863 0.26 -2.5 866,332 + Synpcc7942_0863 0.26 -1.7 866,387 - Synpcc7942_0863 0.30 -0.6 866,390 + Synpcc7942_0863 0.31 -4.1 866,397 - Synpcc7942_0863 0.31 -6.0 866,412 + Synpcc7942_0863 0.33 -1.0 866,423 - Synpcc7942_0863 0.34 -2.2 866,456 - Synpcc7942_0863 0.36 -1.8 866,458 + Synpcc7942_0863 0.36 -0.2 866,458 + -2.3 866,466 - Synpcc7942_0863 0.37 -1.2 866,474 - Synpcc7942_0863 0.38 -2.4 866,491 + Synpcc7942_0863 0.39 -2.1 866,507 - Synpcc7942_0863 0.41 -3.6 866,525 + Synpcc7942_0863 0.42 -4.6 866,525 + Synpcc7942_0863 0.42 -5.6 866,533 - Synpcc7942_0863 0.43 -2.8 866,533 - Synpcc7942_0863 0.43 -3.1 866,560 + Synpcc7942_0863 0.45 -2.4 866,583 - Synpcc7942_0863 0.47 -2.3 866,617 + Synpcc7942_0863 0.50 -3.5 866,617 + Synpcc7942_0863 0.50 -2.3 866,632 + Synpcc7942_0863 0.51 -0.2 866,632 + Synpcc7942_0863 0.51 -4.2 866,640 - Synpcc7942_0863 0.52 -0.7 866,640 - -1.0 866,644 + Synpcc7942_0863 0.52 -5.1 866,655 + Synpcc7942_0863 0.53 -1.2 866,663 - Synpcc7942_0863 0.54 -4.4 866,684 - Synpcc7942_0863 0.56 -2.5 866,733 + Synpcc7942_0863 0.60 -3.5 866,738 + Synpcc7942_0863 0.60 -2.0 866,738 + Synpcc7942_0863 0.60 -2.8 866,746 - Synpcc7942_0863 0.61 -4.7 866,757 + -1.6 866,765 - Synpcc7942_0863 0.62 -1.6 866,765 - Synpcc7942_0863 0.62 -4.3 866,776 + Synpcc7942_0863 0.63 -1.7 866,776 + Synpcc7942_0863 0.63 -1.0 866,776 + Synpcc7942_0863 0.63 -2.0 866,789 - Synpcc7942_0863 0.64 -4.1 866,789 - Synpcc7942_0863 0.64 -1.2 866,822 + Synpcc7942_0863 0.67 -3.1 866,822 + Synpcc7942_0863 0.67 -2.3 866,882 + Synpcc7942_0863 0.72 -3.8 866,890 - Synpcc7942_0863 0.73 -2.9 866,890 - Synpcc7942_0863 0.73 -2.9 866,890 - Synpcc7942_0863 0.73 -1.8 866,890 - Synpcc7942_0863 0.73 -2.8 866,890 - Synpcc7942_0863 0.73 -4.2 866,902 + Synpcc7942_0863 0.74 -3.9 867,014 - Synpcc7942_0863 0.84 -4.2 867,088 - Synpcc7942_0863 0.90 -4.0 867,109 + -2.3 867,129 + -3.1 867,146 + -1.2 867,156 + -2.4 867,188 - -4.9 867,221 - +0.2 867,221 - +1.7 867,239 - +1.8 867,301 + +0.5 867,301 + -0.8 867,301 + -1.8 867,301 + -1.1 867,301 + -0.5 867,301 + +0.2 867,301 + +4.2 867,301 + -1.0 867,301 + -0.4 867,301 + +1.6 867,301 + -1.0 867,304 - -0.6 867,309 - -0.6 867,309 - +2.2 867,309 - -3.3 867,309 - +1.3 867,309 - +2.3 867,309 - -1.2 867,309 - +2.9 867,309 - +1.9 867,309 - -2.7 867,309 - +0.4 867,309 - +0.4 867,309 - -0.8 867,309 - -1.8 867,309 - -1.1 867,311 + -1.3 867,311 + -1.5 867,314 - -0.6 867,314 - +0.2 867,319 - -0.9 867,329 - -0.0 867,329 - +1.8 867,332 + -2.3 867,332 + -2.0 867,332 + -0.8 867,339 - +1.9 867,347 + -0.6 867,347 + -2.7 867,347 + +2.0 867,403 + -2.8 867,415 + -1.4 867,421 + -0.8 867,429 - -2.6 867,481 + Synpcc7942_0864 0.17 -1.7 867,769 + -1.5 867,769 + -2.7 867,917 + -1.1 867,925 - +1.5 867,925 - +0.5 867,926 + +0.1 867,934 - -0.2 867,963 - -2.0 868,029 + +0.8 868,029 + -1.8 868,049 + Synpcc7942_0865 0.13 -2.5 868,068 + Synpcc7942_0865 0.17 +2.9 868,107 + Synpcc7942_0865 0.25 -1.2
Or see this region's nucleotide sequence