Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and Synpcc7942_0836 are separated by 377 nucleotides Synpcc7942_0836 and Synpcc7942_0837 overlap by 4 nucleotides Synpcc7942_0837 and Synpcc7942_0838 are separated by 48 nucleotides
Synpcc7942_0835: ruvB - Holliday junction DNA helicase B, at 829,661 to 830,788
ruvB
Synpcc7942_0836: Synpcc7942_0836 - hypothetical protein, at 831,166 to 832,182
_0836
Synpcc7942_0837: Synpcc7942_0837 - hypothetical protein, at 832,179 to 832,811
_0837
Synpcc7942_0838: Synpcc7942_0838 - Elongator protein 3/MiaB/NifB, at 832,860 to 833,957
_0838
Position (kb) 831
832
833 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 830.773 kb on - strand at 830.840 kb on + strand at 830.850 kb on + strand at 830.881 kb on + strand at 830.881 kb on - strand at 830.888 kb on + strand at 830.893 kb on + strand at 830.897 kb on + strand at 830.901 kb on - strand at 830.908 kb on - strand at 830.945 kb on + strand at 830.986 kb on - strand at 831.011 kb on + strand at 831.016 kb on + strand at 831.029 kb on - strand at 831.077 kb on + strand at 831.078 kb on + strand at 831.093 kb on - strand at 831.159 kb on + strand at 831.159 kb on + strand at 831.159 kb on + strand at 831.159 kb on + strand at 831.161 kb on + strand at 831.167 kb on - strand at 831.167 kb on - strand at 831.167 kb on - strand at 831.177 kb on - strand at 831.192 kb on + strand at 831.210 kb on - strand at 831.214 kb on - strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.216 kb on + strand at 831.224 kb on - strand at 831.224 kb on - strand at 831.224 kb on - strand at 831.224 kb on - strand at 831.224 kb on - strand at 831.224 kb on - strand at 831.224 kb on - strand at 831.224 kb on - strand at 831.224 kb on - strand at 831.224 kb on - strand at 831.224 kb on - strand at 831.224 kb on - strand at 831.228 kb on + strand at 831.246 kb on - strand at 831.273 kb on - strand, within Synpcc7942_0836 at 831.287 kb on + strand, within Synpcc7942_0836 at 831.289 kb on + strand, within Synpcc7942_0836 at 831.297 kb on - strand, within Synpcc7942_0836 at 831.309 kb on + strand, within Synpcc7942_0836 at 831.312 kb on - strand, within Synpcc7942_0836 at 831.353 kb on + strand, within Synpcc7942_0836 at 831.362 kb on - strand, within Synpcc7942_0836 at 831.362 kb on - strand, within Synpcc7942_0836 at 831.423 kb on - strand, within Synpcc7942_0836 at 831.489 kb on - strand, within Synpcc7942_0836 at 831.489 kb on - strand, within Synpcc7942_0836 at 831.489 kb on - strand, within Synpcc7942_0836 at 831.489 kb on - strand, within Synpcc7942_0836 at 831.489 kb on - strand, within Synpcc7942_0836 at 831.499 kb on - strand, within Synpcc7942_0836 at 831.518 kb on + strand, within Synpcc7942_0836 at 831.620 kb on + strand, within Synpcc7942_0836 at 831.634 kb on + strand, within Synpcc7942_0836 at 831.637 kb on - strand, within Synpcc7942_0836 at 831.637 kb on - strand, within Synpcc7942_0836 at 831.650 kb on - strand, within Synpcc7942_0836 at 831.684 kb on - strand, within Synpcc7942_0836 at 831.691 kb on + strand, within Synpcc7942_0836 at 831.707 kb on - strand, within Synpcc7942_0836 at 831.712 kb on + strand, within Synpcc7942_0836 at 831.718 kb on + strand, within Synpcc7942_0836 at 831.718 kb on + strand, within Synpcc7942_0836 at 831.718 kb on + strand, within Synpcc7942_0836 at 831.718 kb on + strand, within Synpcc7942_0836 at 831.718 kb on + strand, within Synpcc7942_0836 at 831.726 kb on - strand, within Synpcc7942_0836 at 831.726 kb on - strand, within Synpcc7942_0836 at 831.726 kb on - strand, within Synpcc7942_0836 at 831.728 kb on + strand, within Synpcc7942_0836 at 831.736 kb on - strand, within Synpcc7942_0836 at 831.767 kb on - strand, within Synpcc7942_0836 at 831.771 kb on - strand, within Synpcc7942_0836 at 831.823 kb on + strand, within Synpcc7942_0836 at 831.823 kb on + strand, within Synpcc7942_0836 at 831.825 kb on + strand, within Synpcc7942_0836 at 831.831 kb on - strand, within Synpcc7942_0836 at 831.833 kb on - strand, within Synpcc7942_0836 at 831.848 kb on + strand, within Synpcc7942_0836 at 831.856 kb on - strand, within Synpcc7942_0836 at 831.858 kb on - strand, within Synpcc7942_0836 at 831.860 kb on + strand, within Synpcc7942_0836 at 831.860 kb on + strand, within Synpcc7942_0836 at 831.860 kb on + strand, within Synpcc7942_0836 at 831.860 kb on + strand, within Synpcc7942_0836 at 831.860 kb on + strand, within Synpcc7942_0836 at 831.860 kb on + strand, within Synpcc7942_0836 at 831.860 kb on + strand, within Synpcc7942_0836 at 831.862 kb on + strand, within Synpcc7942_0836 at 831.868 kb on - strand, within Synpcc7942_0836 at 831.868 kb on - strand, within Synpcc7942_0836 at 831.923 kb on - strand, within Synpcc7942_0836 at 831.926 kb on - strand, within Synpcc7942_0836 at 831.951 kb on - strand, within Synpcc7942_0836 at 831.964 kb on + strand, within Synpcc7942_0836 at 831.975 kb on - strand, within Synpcc7942_0836 at 831.982 kb on + strand, within Synpcc7942_0836 at 832.017 kb on + strand, within Synpcc7942_0836 at 832.017 kb on - strand, within Synpcc7942_0836 at 832.030 kb on + strand, within Synpcc7942_0836 at 832.030 kb on + strand, within Synpcc7942_0836 at 832.030 kb on + strand, within Synpcc7942_0836 at 832.030 kb on + strand, within Synpcc7942_0836 at 832.031 kb on + strand, within Synpcc7942_0836 at 832.038 kb on - strand, within Synpcc7942_0836 at 832.038 kb on - strand, within Synpcc7942_0836 at 832.038 kb on - strand, within Synpcc7942_0836 at 832.080 kb on - strand, within Synpcc7942_0836 at 832.132 kb on + strand at 832.134 kb on - strand at 832.156 kb on + strand at 832.194 kb on - strand at 832.199 kb on + strand at 832.237 kb on + strand at 832.389 kb on - strand, within Synpcc7942_0837 at 832.394 kb on - strand, within Synpcc7942_0837 at 832.439 kb on + strand, within Synpcc7942_0837 at 832.455 kb on + strand, within Synpcc7942_0837 at 832.493 kb on - strand, within Synpcc7942_0837 at 832.514 kb on - strand, within Synpcc7942_0837 at 832.559 kb on - strand, within Synpcc7942_0837 at 832.583 kb on + strand, within Synpcc7942_0837 at 832.616 kb on + strand, within Synpcc7942_0837 at 832.616 kb on + strand at 832.719 kb on - strand, within Synpcc7942_0837 at 832.734 kb on - strand, within Synpcc7942_0837 at 832.734 kb on - strand, within Synpcc7942_0837 at 832.783 kb on - strand at 832.789 kb on - strand at 832.789 kb on - strand at 832.789 kb on - strand at 832.789 kb on - strand at 832.794 kb on - strand at 832.794 kb on - strand at 832.847 kb on + strand at 832.867 kb on + strand at 832.877 kb on + strand at 832.922 kb on - strand at 832.952 kb on - strand at 832.953 kb on + strand at 832.961 kb on - strand at 832.961 kb on - strand at 832.984 kb on - strand, within Synpcc7942_0838 at 833.051 kb on + strand, within Synpcc7942_0838 at 833.097 kb on - strand, within Synpcc7942_0838 at 833.114 kb on - strand, within Synpcc7942_0838 at 833.160 kb on - strand, within Synpcc7942_0838 at 833.166 kb on - strand, within Synpcc7942_0838 at 833.166 kb on - strand, within Synpcc7942_0838 at 833.166 kb on - strand, within Synpcc7942_0838 at 833.168 kb on + strand, within Synpcc7942_0838 at 833.168 kb on + strand, within Synpcc7942_0838 at 833.168 kb on + strand, within Synpcc7942_0838 at 833.168 kb on + strand, within Synpcc7942_0838 at 833.168 kb on + strand, within Synpcc7942_0838 at 833.168 kb on + strand, within Synpcc7942_0838 at 833.176 kb on - strand, within Synpcc7942_0838 at 833.176 kb on - strand, within Synpcc7942_0838 at 833.176 kb on - strand, within Synpcc7942_0838 at 833.179 kb on + strand, within Synpcc7942_0838
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 830,773 - +0.7 830,840 + +0.8 830,850 + +1.0 830,881 + +0.2 830,881 - +2.7 830,888 + -0.6 830,893 + +1.6 830,897 + -2.3 830,901 - -0.8 830,908 - -0.9 830,945 + -0.9 830,986 - -0.4 831,011 + +0.2 831,016 + -0.6 831,029 - -0.1 831,077 + -2.0 831,078 + -0.0 831,093 - -2.4 831,159 + +1.0 831,159 + +1.2 831,159 + -0.4 831,159 + +0.6 831,161 + -0.4 831,167 - +1.7 831,167 - -1.6 831,167 - +0.0 831,177 - -0.2 831,192 + +1.3 831,210 - +1.8 831,214 - -1.4 831,216 + -0.3 831,216 + +0.8 831,216 + +1.3 831,216 + -1.6 831,216 + -0.3 831,216 + +0.4 831,216 + +0.4 831,216 + -1.5 831,216 + -1.8 831,216 + -0.2 831,216 + +0.3 831,216 + -0.4 831,216 + +2.6 831,216 + +0.7 831,216 + +2.3 831,216 + +1.3 831,216 + -0.6 831,216 + -1.5 831,216 + -1.8 831,216 + +2.4 831,216 + +1.1 831,216 + -2.8 831,224 - -1.9 831,224 - +0.4 831,224 - -1.1 831,224 - +0.8 831,224 - +1.3 831,224 - +0.8 831,224 - +0.2 831,224 - +2.7 831,224 - +0.3 831,224 - -1.5 831,224 - +1.9 831,224 - -2.1 831,228 + -2.0 831,246 - -1.5 831,273 - Synpcc7942_0836 0.11 -0.6 831,287 + Synpcc7942_0836 0.12 +1.5 831,289 + Synpcc7942_0836 0.12 -0.5 831,297 - Synpcc7942_0836 0.13 -0.6 831,309 + Synpcc7942_0836 0.14 +3.5 831,312 - Synpcc7942_0836 0.14 -3.1 831,353 + Synpcc7942_0836 0.18 +1.7 831,362 - Synpcc7942_0836 0.19 -1.6 831,362 - Synpcc7942_0836 0.19 -0.9 831,423 - Synpcc7942_0836 0.25 -0.4 831,489 - Synpcc7942_0836 0.32 +0.2 831,489 - Synpcc7942_0836 0.32 -2.6 831,489 - Synpcc7942_0836 0.32 -2.6 831,489 - Synpcc7942_0836 0.32 -1.8 831,489 - Synpcc7942_0836 0.32 -2.2 831,499 - Synpcc7942_0836 0.33 +0.1 831,518 + Synpcc7942_0836 0.35 +0.1 831,620 + Synpcc7942_0836 0.45 +1.3 831,634 + Synpcc7942_0836 0.46 +1.3 831,637 - Synpcc7942_0836 0.46 +1.8 831,637 - Synpcc7942_0836 0.46 -1.8 831,650 - Synpcc7942_0836 0.48 -0.4 831,684 - Synpcc7942_0836 0.51 +1.3 831,691 + Synpcc7942_0836 0.52 +0.6 831,707 - Synpcc7942_0836 0.53 +1.1 831,712 + Synpcc7942_0836 0.54 -1.2 831,718 + Synpcc7942_0836 0.54 -3.2 831,718 + Synpcc7942_0836 0.54 -0.2 831,718 + Synpcc7942_0836 0.54 -0.0 831,718 + Synpcc7942_0836 0.54 +1.1 831,718 + Synpcc7942_0836 0.54 -2.5 831,726 - Synpcc7942_0836 0.55 -1.8 831,726 - Synpcc7942_0836 0.55 -0.7 831,726 - Synpcc7942_0836 0.55 +1.0 831,728 + Synpcc7942_0836 0.55 -2.1 831,736 - Synpcc7942_0836 0.56 -3.0 831,767 - Synpcc7942_0836 0.59 +0.6 831,771 - Synpcc7942_0836 0.59 -0.2 831,823 + Synpcc7942_0836 0.65 +0.5 831,823 + Synpcc7942_0836 0.65 +0.2 831,825 + Synpcc7942_0836 0.65 +0.6 831,831 - Synpcc7942_0836 0.65 -0.4 831,833 - Synpcc7942_0836 0.66 -0.4 831,848 + Synpcc7942_0836 0.67 +4.1 831,856 - Synpcc7942_0836 0.68 -3.9 831,858 - Synpcc7942_0836 0.68 +0.3 831,860 + Synpcc7942_0836 0.68 -1.8 831,860 + Synpcc7942_0836 0.68 +0.1 831,860 + Synpcc7942_0836 0.68 +2.8 831,860 + Synpcc7942_0836 0.68 -1.3 831,860 + Synpcc7942_0836 0.68 -1.0 831,860 + Synpcc7942_0836 0.68 +2.0 831,860 + Synpcc7942_0836 0.68 -0.8 831,862 + Synpcc7942_0836 0.68 +0.3 831,868 - Synpcc7942_0836 0.69 +0.9 831,868 - Synpcc7942_0836 0.69 -0.6 831,923 - Synpcc7942_0836 0.74 +1.4 831,926 - Synpcc7942_0836 0.75 +1.5 831,951 - Synpcc7942_0836 0.77 -1.0 831,964 + Synpcc7942_0836 0.78 -2.1 831,975 - Synpcc7942_0836 0.80 -0.8 831,982 + Synpcc7942_0836 0.80 -0.6 832,017 + Synpcc7942_0836 0.84 +0.4 832,017 - Synpcc7942_0836 0.84 -2.6 832,030 + Synpcc7942_0836 0.85 +0.4 832,030 + Synpcc7942_0836 0.85 -2.6 832,030 + Synpcc7942_0836 0.85 -0.0 832,030 + Synpcc7942_0836 0.85 -0.8 832,031 + Synpcc7942_0836 0.85 -0.7 832,038 - Synpcc7942_0836 0.86 -2.2 832,038 - Synpcc7942_0836 0.86 +2.0 832,038 - Synpcc7942_0836 0.86 -1.3 832,080 - Synpcc7942_0836 0.90 -0.5 832,132 + +0.4 832,134 - +1.2 832,156 + +0.2 832,194 - +2.6 832,199 + -0.8 832,237 + +1.8 832,389 - Synpcc7942_0837 0.33 -0.6 832,394 - Synpcc7942_0837 0.34 -2.6 832,439 + Synpcc7942_0837 0.41 +0.6 832,455 + Synpcc7942_0837 0.44 -3.2 832,493 - Synpcc7942_0837 0.50 -0.4 832,514 - Synpcc7942_0837 0.53 +2.2 832,559 - Synpcc7942_0837 0.60 +1.3 832,583 + Synpcc7942_0837 0.64 +1.0 832,616 + Synpcc7942_0837 0.69 -3.2 832,616 + -0.0 832,719 - Synpcc7942_0837 0.85 +8.8 832,734 - Synpcc7942_0837 0.88 -0.9 832,734 - Synpcc7942_0837 0.88 -1.9 832,783 - +0.7 832,789 - +0.6 832,789 - +1.5 832,789 - -0.6 832,789 - +0.5 832,794 - +2.6 832,794 - -2.5 832,847 + +1.4 832,867 + +0.7 832,877 + +0.4 832,922 - +0.1 832,952 - +2.1 832,953 + +1.1 832,961 - +1.0 832,961 - -1.2 832,984 - Synpcc7942_0838 0.11 -1.7 833,051 + Synpcc7942_0838 0.17 +0.7 833,097 - Synpcc7942_0838 0.22 -1.5 833,114 - Synpcc7942_0838 0.23 +0.3 833,160 - Synpcc7942_0838 0.27 +0.5 833,166 - Synpcc7942_0838 0.28 +0.1 833,166 - Synpcc7942_0838 0.28 -2.5 833,166 - Synpcc7942_0838 0.28 +0.2 833,168 + Synpcc7942_0838 0.28 -1.7 833,168 + Synpcc7942_0838 0.28 -0.5 833,168 + Synpcc7942_0838 0.28 +0.6 833,168 + Synpcc7942_0838 0.28 +2.0 833,168 + Synpcc7942_0838 0.28 +2.0 833,168 + Synpcc7942_0838 0.28 -0.3 833,176 - Synpcc7942_0838 0.29 +2.9 833,176 - Synpcc7942_0838 0.29 -3.3 833,176 - Synpcc7942_0838 0.29 +0.7 833,179 + Synpcc7942_0838 0.29 -2.8
Or see this region's nucleotide sequence