Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0816 and Synpcc7942_0817 are separated by 104 nucleotides Synpcc7942_0817 and purS are separated by 231 nucleotides purS and purL overlap by 4 nucleotides
Synpcc7942_0816: Synpcc7942_0816 - diguanylate cyclase/phosphodiesterase, at 810,181 to 812,226
_0816
Synpcc7942_0817: Synpcc7942_0817 - putative ferric uptake regulator, FUR family, at 812,331 to 812,723
_0817
Synpcc7942_0818: purS - phosphoribosylformylglycinamidine synthase subunit PurS, at 812,955 to 813,224
purS
Synpcc7942_0819: purL - phosphoribosylformylglycinamidine synthase subunit I, at 813,221 to 813,886
purL
Position (kb)
812
813 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 811.361 kb on + strand, within Synpcc7942_0816 at 811.387 kb on - strand, within Synpcc7942_0816 at 811.397 kb on + strand at 811.421 kb on - strand at 811.440 kb on - strand, within Synpcc7942_0816 at 811.445 kb on - strand, within Synpcc7942_0816 at 811.622 kb on + strand, within Synpcc7942_0816 at 811.644 kb on - strand, within Synpcc7942_0816 at 811.650 kb on - strand, within Synpcc7942_0816 at 811.670 kb on + strand, within Synpcc7942_0816 at 811.702 kb on - strand, within Synpcc7942_0816 at 811.702 kb on - strand, within Synpcc7942_0816 at 811.702 kb on - strand, within Synpcc7942_0816 at 811.758 kb on - strand, within Synpcc7942_0816 at 811.865 kb on + strand, within Synpcc7942_0816 at 811.871 kb on + strand, within Synpcc7942_0816 at 811.873 kb on - strand, within Synpcc7942_0816 at 812.156 kb on + strand at 812.217 kb on - strand at 812.227 kb on + strand at 812.375 kb on + strand, within Synpcc7942_0817 at 812.383 kb on - strand, within Synpcc7942_0817 at 812.409 kb on + strand, within Synpcc7942_0817 at 812.420 kb on + strand, within Synpcc7942_0817 at 812.433 kb on + strand, within Synpcc7942_0817 at 812.433 kb on + strand, within Synpcc7942_0817 at 812.438 kb on + strand, within Synpcc7942_0817 at 812.441 kb on - strand, within Synpcc7942_0817 at 812.441 kb on - strand, within Synpcc7942_0817 at 812.473 kb on - strand, within Synpcc7942_0817 at 812.475 kb on - strand, within Synpcc7942_0817 at 812.508 kb on + strand, within Synpcc7942_0817 at 812.515 kb on + strand, within Synpcc7942_0817 at 812.515 kb on + strand, within Synpcc7942_0817 at 812.515 kb on + strand, within Synpcc7942_0817 at 812.523 kb on - strand, within Synpcc7942_0817 at 812.523 kb on - strand, within Synpcc7942_0817 at 812.523 kb on - strand, within Synpcc7942_0817 at 812.528 kb on - strand, within Synpcc7942_0817 at 812.558 kb on + strand, within Synpcc7942_0817 at 812.566 kb on - strand, within Synpcc7942_0817 at 812.568 kb on + strand, within Synpcc7942_0817 at 812.594 kb on - strand, within Synpcc7942_0817 at 812.605 kb on + strand, within Synpcc7942_0817 at 812.621 kb on - strand, within Synpcc7942_0817 at 812.621 kb on - strand, within Synpcc7942_0817 at 812.621 kb on - strand, within Synpcc7942_0817 at 812.638 kb on - strand, within Synpcc7942_0817 at 812.643 kb on - strand, within Synpcc7942_0817 at 812.645 kb on - strand, within Synpcc7942_0817 at 812.651 kb on - strand, within Synpcc7942_0817 at 812.661 kb on - strand, within Synpcc7942_0817 at 812.663 kb on + strand, within Synpcc7942_0817 at 812.673 kb on + strand, within Synpcc7942_0817 at 812.673 kb on + strand, within Synpcc7942_0817 at 812.681 kb on - strand, within Synpcc7942_0817 at 812.718 kb on + strand at 812.718 kb on + strand at 812.718 kb on + strand at 812.725 kb on + strand at 812.727 kb on - strand at 812.731 kb on + strand at 812.744 kb on + strand at 812.745 kb on + strand at 812.745 kb on + strand at 812.759 kb on - strand at 812.774 kb on - strand at 812.778 kb on + strand at 812.779 kb on + strand at 812.793 kb on - strand at 812.793 kb on - strand at 812.838 kb on + strand at 812.846 kb on + strand at 812.851 kb on + strand at 812.854 kb on - strand at 812.873 kb on - strand at 812.893 kb on + strand at 812.904 kb on - strand at 813.219 kb on - strand at 813.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 811,361 + Synpcc7942_0816 0.58 -0.3 811,387 - Synpcc7942_0816 0.59 -1.7 811,397 + -1.2 811,421 - -0.0 811,440 - Synpcc7942_0816 0.62 -2.0 811,445 - Synpcc7942_0816 0.62 -2.3 811,622 + Synpcc7942_0816 0.70 -1.6 811,644 - Synpcc7942_0816 0.72 -2.1 811,650 - Synpcc7942_0816 0.72 -2.5 811,670 + Synpcc7942_0816 0.73 -1.2 811,702 - Synpcc7942_0816 0.74 +0.9 811,702 - Synpcc7942_0816 0.74 -2.5 811,702 - Synpcc7942_0816 0.74 +1.8 811,758 - Synpcc7942_0816 0.77 -2.3 811,865 + Synpcc7942_0816 0.82 +2.7 811,871 + Synpcc7942_0816 0.83 +1.6 811,873 - Synpcc7942_0816 0.83 -2.9 812,156 + +0.8 812,217 - -1.6 812,227 + +3.2 812,375 + Synpcc7942_0817 0.11 -3.2 812,383 - Synpcc7942_0817 0.13 -1.0 812,409 + Synpcc7942_0817 0.20 -1.5 812,420 + Synpcc7942_0817 0.23 -3.4 812,433 + Synpcc7942_0817 0.26 -1.7 812,433 + Synpcc7942_0817 0.26 -4.9 812,438 + Synpcc7942_0817 0.27 -1.9 812,441 - Synpcc7942_0817 0.28 -1.1 812,441 - Synpcc7942_0817 0.28 -4.0 812,473 - Synpcc7942_0817 0.36 -3.0 812,475 - Synpcc7942_0817 0.37 -3.1 812,508 + Synpcc7942_0817 0.45 -2.2 812,515 + Synpcc7942_0817 0.47 -1.6 812,515 + Synpcc7942_0817 0.47 -2.6 812,515 + Synpcc7942_0817 0.47 -1.3 812,523 - Synpcc7942_0817 0.49 -2.1 812,523 - Synpcc7942_0817 0.49 -2.6 812,523 - Synpcc7942_0817 0.49 -2.8 812,528 - Synpcc7942_0817 0.50 -1.1 812,558 + Synpcc7942_0817 0.58 -2.9 812,566 - Synpcc7942_0817 0.60 -3.5 812,568 + Synpcc7942_0817 0.60 -2.4 812,594 - Synpcc7942_0817 0.67 -3.0 812,605 + Synpcc7942_0817 0.70 -1.5 812,621 - Synpcc7942_0817 0.74 -1.4 812,621 - Synpcc7942_0817 0.74 -2.6 812,621 - Synpcc7942_0817 0.74 -1.8 812,638 - Synpcc7942_0817 0.78 -1.2 812,643 - Synpcc7942_0817 0.79 -2.7 812,645 - Synpcc7942_0817 0.80 -4.0 812,651 - Synpcc7942_0817 0.81 -2.1 812,661 - Synpcc7942_0817 0.84 -1.8 812,663 + Synpcc7942_0817 0.84 -3.9 812,673 + Synpcc7942_0817 0.87 -0.8 812,673 + Synpcc7942_0817 0.87 -1.0 812,681 - Synpcc7942_0817 0.89 -1.9 812,718 + -2.1 812,718 + +0.6 812,718 + +2.3 812,725 + -1.7 812,727 - -0.0 812,731 + -2.2 812,744 + -0.8 812,745 + -2.5 812,745 + -3.8 812,759 - +2.2 812,774 - -2.7 812,778 + +2.7 812,779 + -4.1 812,793 - -0.6 812,793 - +1.7 812,838 + -3.9 812,846 + -2.7 812,851 + -1.5 812,854 - -0.3 812,873 - -1.2 812,893 + +0.6 812,904 - -0.0 813,219 - +0.7 813,219 - -1.7
Or see this region's nucleotide sequence