Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt act and Synpcc7942_0705 overlap by 14 nucleotides Synpcc7942_0705 and cobL are separated by 6 nucleotides cobL and cad overlap by 20 nucleotides
Synpcc7942_0704: act - Chloramphenicol O-acetyltransferase, at 695,313 to 695,948
act
Synpcc7942_0705: Synpcc7942_0705 - hypothetical protein, at 695,935 to 696,570
_0705
Synpcc7942_0706: cobL - precorrin-6B methylase, at 696,577 to 697,191
cobL
Synpcc7942_0707: cad - arginine decarboxylase, at 697,172 to 698,641
cad
Position (kb) 696
697
698 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 695.835 kb on + strand, within act at 696.145 kb on + strand, within Synpcc7942_0705 at 696.164 kb on + strand, within Synpcc7942_0705 at 696.209 kb on - strand, within Synpcc7942_0705 at 696.213 kb on + strand, within Synpcc7942_0705 at 696.301 kb on - strand, within Synpcc7942_0705 at 696.371 kb on - strand, within Synpcc7942_0705 at 696.387 kb on + strand, within Synpcc7942_0705 at 696.397 kb on + strand, within Synpcc7942_0705 at 696.397 kb on + strand, within Synpcc7942_0705 at 696.403 kb on - strand, within Synpcc7942_0705 at 696.464 kb on + strand, within Synpcc7942_0705 at 696.464 kb on - strand, within Synpcc7942_0705 at 696.466 kb on + strand, within Synpcc7942_0705 at 696.474 kb on - strand, within Synpcc7942_0705 at 696.474 kb on - strand at 696.474 kb on - strand, within Synpcc7942_0705 at 696.719 kb on + strand, within cobL at 696.722 kb on + strand, within cobL at 696.722 kb on + strand, within cobL at 696.724 kb on + strand, within cobL at 696.748 kb on + strand, within cobL at 696.756 kb on - strand, within cobL at 696.756 kb on - strand, within cobL at 696.770 kb on + strand, within cobL at 696.799 kb on - strand, within cobL at 696.799 kb on - strand, within cobL at 696.837 kb on + strand, within cobL at 696.845 kb on - strand, within cobL at 696.866 kb on - strand, within cobL at 696.888 kb on - strand, within cobL at 696.896 kb on + strand, within cobL at 696.923 kb on - strand, within cobL at 696.926 kb on + strand, within cobL at 696.930 kb on + strand, within cobL at 696.955 kb on - strand, within cobL at 696.983 kb on + strand, within cobL at 696.983 kb on + strand, within cobL at 697.040 kb on + strand, within cobL at 697.040 kb on - strand, within cobL at 697.055 kb on - strand, within cobL at 697.135 kb on - strand at 697.144 kb on + strand at 697.154 kb on + strand at 697.159 kb on + strand at 697.162 kb on - strand at 697.162 kb on - strand at 697.178 kb on - strand at 697.180 kb on + strand at 697.180 kb on + strand at 697.183 kb on - strand at 697.187 kb on + strand at 697.188 kb on - strand at 697.198 kb on + strand at 697.205 kb on + strand at 697.205 kb on + strand at 697.213 kb on - strand at 697.226 kb on + strand at 697.349 kb on + strand at 697.395 kb on - strand, within cad at 697.432 kb on + strand, within cad at 697.500 kb on - strand, within cad at 697.502 kb on - strand, within cad at 697.517 kb on + strand, within cad at 697.519 kb on - strand, within cad at 697.529 kb on - strand, within cad at 697.583 kb on - strand, within cad at 697.678 kb on + strand, within cad at 697.720 kb on + strand, within cad at 697.744 kb on - strand, within cad at 697.828 kb on + strand, within cad at 697.878 kb on - strand, within cad at 697.909 kb on + strand, within cad at 697.914 kb on + strand, within cad at 697.922 kb on - strand, within cad at 697.945 kb on - strand, within cad at 698.042 kb on + strand, within cad at 698.124 kb on + strand, within cad
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 695,835 + act Synpcc7942_0704 0.82 +1.1 696,145 + Synpcc7942_0705 0.33 -3.0 696,164 + Synpcc7942_0705 0.36 -2.2 696,209 - Synpcc7942_0705 0.43 +1.3 696,213 + Synpcc7942_0705 0.44 -2.7 696,301 - Synpcc7942_0705 0.58 +0.8 696,371 - Synpcc7942_0705 0.69 +0.3 696,387 + Synpcc7942_0705 0.71 +0.9 696,397 + Synpcc7942_0705 0.73 -0.7 696,397 + Synpcc7942_0705 0.73 -1.9 696,403 - Synpcc7942_0705 0.74 -1.5 696,464 + Synpcc7942_0705 0.83 +0.7 696,464 - Synpcc7942_0705 0.83 +1.0 696,466 + Synpcc7942_0705 0.83 -2.8 696,474 - Synpcc7942_0705 0.85 -0.3 696,474 - +1.0 696,474 - Synpcc7942_0705 0.85 +1.2 696,719 + cobL Synpcc7942_0706 0.23 -1.3 696,722 + cobL Synpcc7942_0706 0.24 -4.9 696,722 + cobL Synpcc7942_0706 0.24 -6.6 696,724 + cobL Synpcc7942_0706 0.24 -1.5 696,748 + cobL Synpcc7942_0706 0.28 -1.8 696,756 - cobL Synpcc7942_0706 0.29 -2.3 696,756 - cobL Synpcc7942_0706 0.29 -2.1 696,770 + cobL Synpcc7942_0706 0.31 -2.3 696,799 - cobL Synpcc7942_0706 0.36 -4.2 696,799 - cobL Synpcc7942_0706 0.36 -1.2 696,837 + cobL Synpcc7942_0706 0.42 -0.9 696,845 - cobL Synpcc7942_0706 0.44 -2.8 696,866 - cobL Synpcc7942_0706 0.47 -5.7 696,888 - cobL Synpcc7942_0706 0.51 -1.0 696,896 + cobL Synpcc7942_0706 0.52 -3.1 696,923 - cobL Synpcc7942_0706 0.56 -3.3 696,926 + cobL Synpcc7942_0706 0.57 -3.6 696,930 + cobL Synpcc7942_0706 0.57 -2.2 696,955 - cobL Synpcc7942_0706 0.61 +0.7 696,983 + cobL Synpcc7942_0706 0.66 -1.0 696,983 + cobL Synpcc7942_0706 0.66 -3.3 697,040 + cobL Synpcc7942_0706 0.75 -3.4 697,040 - cobL Synpcc7942_0706 0.75 -2.3 697,055 - cobL Synpcc7942_0706 0.78 -4.4 697,135 - -6.0 697,144 + -2.0 697,154 + -3.4 697,159 + -3.9 697,162 - -1.6 697,162 - -2.9 697,178 - -2.6 697,180 + +1.0 697,180 + -0.5 697,183 - -0.0 697,187 + +1.4 697,188 - -5.1 697,198 + +1.4 697,205 + -0.7 697,205 + -2.8 697,213 - -2.5 697,226 + -1.9 697,349 + +0.2 697,395 - cad Synpcc7942_0707 0.15 -1.8 697,432 + cad Synpcc7942_0707 0.18 -2.0 697,500 - cad Synpcc7942_0707 0.22 +0.1 697,502 - cad Synpcc7942_0707 0.22 +2.0 697,517 + cad Synpcc7942_0707 0.23 -1.7 697,519 - cad Synpcc7942_0707 0.24 -0.1 697,529 - cad Synpcc7942_0707 0.24 -1.8 697,583 - cad Synpcc7942_0707 0.28 -1.2 697,678 + cad Synpcc7942_0707 0.34 -1.5 697,720 + cad Synpcc7942_0707 0.37 +0.5 697,744 - cad Synpcc7942_0707 0.39 -0.8 697,828 + cad Synpcc7942_0707 0.45 -0.6 697,878 - cad Synpcc7942_0707 0.48 -1.8 697,909 + cad Synpcc7942_0707 0.50 -0.0 697,914 + cad Synpcc7942_0707 0.50 -0.4 697,922 - cad Synpcc7942_0707 0.51 +1.0 697,945 - cad Synpcc7942_0707 0.53 +1.1 698,042 + cad Synpcc7942_0707 0.59 -1.2 698,124 + cad Synpcc7942_0707 0.65 -2.3
Or see this region's nucleotide sequence