Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvD and tRNA-Gln are separated by 150 nucleotides tRNA-Gln and Synpcc7942_0627 are separated by 18 nucleotides Synpcc7942_0627 and Synpcc7942_0628 are separated by 50 nucleotides Synpcc7942_0628 and Synpcc7942_0629 overlap by 4 nucleotides
Synpcc7942_0626: ilvD - dihydroxy-acid dehydratase, at 618,362 to 620,221
ilvD
Synpcc7942_R0037: tRNA-Gln - tRNA-Gln, at 620,372 to 620,443
tRNA-Gln
Synpcc7942_0627: Synpcc7942_0627 - hypothetical protein, at 620,462 to 621,226
_0627
Synpcc7942_0628: Synpcc7942_0628 - spermidine synthase, at 621,277 to 622,137
_0628
Synpcc7942_0629: Synpcc7942_0629 - hypothetical protein, at 622,134 to 622,340
_0629
Position (kb) 620
621
622 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 620.232 kb on + strand at 620.234 kb on + strand at 620.242 kb on - strand at 620.247 kb on + strand at 620.250 kb on - strand at 620.260 kb on - strand at 620.283 kb on - strand at 620.288 kb on - strand at 620.438 kb on + strand at 620.451 kb on - strand at 620.467 kb on - strand at 620.570 kb on + strand, within Synpcc7942_0627 at 620.574 kb on + strand, within Synpcc7942_0627 at 620.586 kb on + strand, within Synpcc7942_0627 at 620.594 kb on - strand, within Synpcc7942_0627 at 620.594 kb on - strand, within Synpcc7942_0627 at 620.629 kb on + strand, within Synpcc7942_0627 at 620.629 kb on + strand, within Synpcc7942_0627 at 620.629 kb on + strand, within Synpcc7942_0627 at 620.629 kb on + strand, within Synpcc7942_0627 at 620.629 kb on + strand, within Synpcc7942_0627 at 620.637 kb on - strand, within Synpcc7942_0627 at 620.637 kb on - strand, within Synpcc7942_0627 at 620.657 kb on + strand, within Synpcc7942_0627 at 620.686 kb on + strand, within Synpcc7942_0627 at 620.694 kb on - strand, within Synpcc7942_0627 at 620.708 kb on - strand, within Synpcc7942_0627 at 620.710 kb on - strand, within Synpcc7942_0627 at 620.731 kb on - strand, within Synpcc7942_0627 at 620.745 kb on + strand, within Synpcc7942_0627 at 620.753 kb on - strand, within Synpcc7942_0627 at 620.807 kb on - strand, within Synpcc7942_0627 at 620.811 kb on + strand, within Synpcc7942_0627 at 620.835 kb on - strand, within Synpcc7942_0627 at 620.878 kb on - strand, within Synpcc7942_0627 at 620.924 kb on - strand, within Synpcc7942_0627 at 620.953 kb on - strand, within Synpcc7942_0627 at 620.993 kb on - strand, within Synpcc7942_0627 at 621.078 kb on - strand, within Synpcc7942_0627 at 621.106 kb on + strand, within Synpcc7942_0627 at 621.106 kb on - strand, within Synpcc7942_0627 at 621.111 kb on - strand, within Synpcc7942_0627 at 621.175 kb on + strand at 621.212 kb on + strand at 621.223 kb on + strand at 621.228 kb on - strand at 621.233 kb on + strand at 621.235 kb on + strand at 621.240 kb on + strand at 621.243 kb on - strand at 621.248 kb on - strand at 621.248 kb on - strand at 621.248 kb on - strand at 621.251 kb on + strand at 621.256 kb on + strand at 621.259 kb on - strand at 621.261 kb on + strand at 621.274 kb on + strand at 621.352 kb on - strand at 621.657 kb on + strand, within Synpcc7942_0628 at 621.704 kb on + strand, within Synpcc7942_0628 at 621.712 kb on - strand, within Synpcc7942_0628 at 621.716 kb on - strand, within Synpcc7942_0628 at 621.821 kb on + strand, within Synpcc7942_0628 at 621.885 kb on + strand, within Synpcc7942_0628 at 621.930 kb on + strand, within Synpcc7942_0628 at 621.995 kb on + strand, within Synpcc7942_0628 at 622.093 kb on - strand at 622.113 kb on + strand at 622.122 kb on - strand at 622.126 kb on + strand at 622.140 kb on - strand at 622.163 kb on + strand, within Synpcc7942_0629 at 622.175 kb on - strand, within Synpcc7942_0629 at 622.200 kb on + strand, within Synpcc7942_0629 at 622.209 kb on + strand, within Synpcc7942_0629 at 622.216 kb on + strand, within Synpcc7942_0629 at 622.217 kb on - strand, within Synpcc7942_0629 at 622.217 kb on - strand, within Synpcc7942_0629 at 622.221 kb on + strand, within Synpcc7942_0629 at 622.222 kb on - strand, within Synpcc7942_0629
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 620,232 + +1.0 620,234 + +0.7 620,242 - +0.0 620,247 + +0.0 620,250 - +0.2 620,260 - +0.5 620,283 - +0.3 620,288 - +1.3 620,438 + +2.1 620,451 - +1.7 620,467 - -1.2 620,570 + Synpcc7942_0627 0.14 -2.6 620,574 + Synpcc7942_0627 0.15 -4.4 620,586 + Synpcc7942_0627 0.16 -1.4 620,594 - Synpcc7942_0627 0.17 -2.5 620,594 - Synpcc7942_0627 0.17 -2.7 620,629 + Synpcc7942_0627 0.22 -2.6 620,629 + Synpcc7942_0627 0.22 -2.3 620,629 + Synpcc7942_0627 0.22 -1.4 620,629 + Synpcc7942_0627 0.22 -2.4 620,629 + Synpcc7942_0627 0.22 -1.2 620,637 - Synpcc7942_0627 0.23 -1.9 620,637 - Synpcc7942_0627 0.23 -2.4 620,657 + Synpcc7942_0627 0.25 -2.5 620,686 + Synpcc7942_0627 0.29 -2.5 620,694 - Synpcc7942_0627 0.30 -1.5 620,708 - Synpcc7942_0627 0.32 -3.0 620,710 - Synpcc7942_0627 0.32 -1.4 620,731 - Synpcc7942_0627 0.35 -2.0 620,745 + Synpcc7942_0627 0.37 -1.4 620,753 - Synpcc7942_0627 0.38 -2.5 620,807 - Synpcc7942_0627 0.45 -1.4 620,811 + Synpcc7942_0627 0.46 -0.6 620,835 - Synpcc7942_0627 0.49 -1.3 620,878 - Synpcc7942_0627 0.54 -2.3 620,924 - Synpcc7942_0627 0.60 -1.5 620,953 - Synpcc7942_0627 0.64 -3.4 620,993 - Synpcc7942_0627 0.69 -0.5 621,078 - Synpcc7942_0627 0.81 -1.5 621,106 + Synpcc7942_0627 0.84 -4.3 621,106 - Synpcc7942_0627 0.84 -3.6 621,111 - Synpcc7942_0627 0.85 -2.9 621,175 + +0.0 621,212 + -1.5 621,223 + +1.2 621,228 - +0.7 621,233 + -2.6 621,235 + -2.4 621,240 + -2.4 621,243 - -0.2 621,248 - +0.5 621,248 - -1.4 621,248 - -0.5 621,251 + +0.1 621,256 + +3.6 621,259 - +0.8 621,261 + +0.4 621,274 + -0.9 621,352 - -2.1 621,657 + Synpcc7942_0628 0.44 -0.8 621,704 + Synpcc7942_0628 0.50 +3.0 621,712 - Synpcc7942_0628 0.51 -0.4 621,716 - Synpcc7942_0628 0.51 -0.4 621,821 + Synpcc7942_0628 0.63 -1.0 621,885 + Synpcc7942_0628 0.71 -1.2 621,930 + Synpcc7942_0628 0.76 -1.5 621,995 + Synpcc7942_0628 0.83 -1.2 622,093 - -1.4 622,113 + +2.4 622,122 - +3.8 622,126 + -0.3 622,140 - -2.5 622,163 + Synpcc7942_0629 0.14 -2.6 622,175 - Synpcc7942_0629 0.20 +0.4 622,200 + Synpcc7942_0629 0.32 -2.8 622,209 + Synpcc7942_0629 0.36 +4.3 622,216 + Synpcc7942_0629 0.40 +2.4 622,217 - Synpcc7942_0629 0.40 -0.1 622,217 - Synpcc7942_0629 0.40 +0.5 622,221 + Synpcc7942_0629 0.42 +0.0 622,222 - Synpcc7942_0629 0.43 -0.4
Or see this region's nucleotide sequence