Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gltA and sixA are separated by 134 nucleotides sixA and Synpcc7942_0614 are separated by 3 nucleotides
Synpcc7942_0612: gltA - methylcitrate synthase, at 602,209 to 603,369
gltA
Synpcc7942_0613: sixA - phosphohistidine phosphatase, SixA, at 603,504 to 603,992
sixA
Synpcc7942_0614: Synpcc7942_0614 - hypothetical protein, at 603,996 to 605,675
_0614
Position (kb) 603
604 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 603.455 kb on - strand at 603.490 kb on + strand at 603.510 kb on - strand at 603.510 kb on - strand at 603.510 kb on - strand at 603.510 kb on - strand at 603.529 kb on + strand at 603.569 kb on - strand, within sixA at 603.583 kb on - strand, within sixA at 603.603 kb on - strand, within sixA at 603.603 kb on - strand, within sixA at 603.615 kb on + strand, within sixA at 603.615 kb on + strand, within sixA at 603.615 kb on + strand, within sixA at 603.618 kb on - strand, within sixA at 603.623 kb on - strand, within sixA at 603.623 kb on - strand, within sixA at 603.641 kb on + strand, within sixA at 603.661 kb on - strand, within sixA at 603.661 kb on - strand, within sixA at 603.661 kb on - strand, within sixA at 603.687 kb on + strand, within sixA at 603.687 kb on + strand, within sixA at 603.732 kb on + strand, within sixA at 603.780 kb on + strand, within sixA at 603.825 kb on + strand, within sixA at 603.828 kb on - strand, within sixA at 603.838 kb on + strand, within sixA at 603.845 kb on - strand, within sixA at 603.850 kb on + strand, within sixA at 603.905 kb on + strand, within sixA at 603.913 kb on + strand, within sixA at 603.994 kb on + strand at 604.037 kb on - strand at 604.052 kb on - strand at 604.544 kb on - strand, within Synpcc7942_0614 at 604.686 kb on + strand, within Synpcc7942_0614
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 603,455 - +1.0 603,490 + +0.9 603,510 - -0.6 603,510 - -1.9 603,510 - -3.4 603,510 - -0.8 603,529 + +2.3 603,569 - sixA Synpcc7942_0613 0.13 -0.0 603,583 - sixA Synpcc7942_0613 0.16 +1.5 603,603 - sixA Synpcc7942_0613 0.20 -1.5 603,603 - sixA Synpcc7942_0613 0.20 +1.4 603,615 + sixA Synpcc7942_0613 0.23 +0.9 603,615 + sixA Synpcc7942_0613 0.23 -1.2 603,615 + sixA Synpcc7942_0613 0.23 -2.3 603,618 - sixA Synpcc7942_0613 0.23 +1.9 603,623 - sixA Synpcc7942_0613 0.24 -0.8 603,623 - sixA Synpcc7942_0613 0.24 +1.8 603,641 + sixA Synpcc7942_0613 0.28 -1.0 603,661 - sixA Synpcc7942_0613 0.32 -0.5 603,661 - sixA Synpcc7942_0613 0.32 -2.7 603,661 - sixA Synpcc7942_0613 0.32 -2.0 603,687 + sixA Synpcc7942_0613 0.37 -2.3 603,687 + sixA Synpcc7942_0613 0.37 -3.2 603,732 + sixA Synpcc7942_0613 0.47 -1.1 603,780 + sixA Synpcc7942_0613 0.56 -1.6 603,825 + sixA Synpcc7942_0613 0.66 +2.6 603,828 - sixA Synpcc7942_0613 0.66 +0.8 603,838 + sixA Synpcc7942_0613 0.68 +0.1 603,845 - sixA Synpcc7942_0613 0.70 -1.8 603,850 + sixA Synpcc7942_0613 0.71 -2.0 603,905 + sixA Synpcc7942_0613 0.82 +0.2 603,913 + sixA Synpcc7942_0613 0.84 -2.0 603,994 + -0.4 604,037 - -0.2 604,052 - +0.1 604,544 - Synpcc7942_0614 0.33 +0.4 604,686 + Synpcc7942_0614 0.41 -0.1
Or see this region's nucleotide sequence