Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aat and Synpcc7942_0607 are separated by 13 nucleotides Synpcc7942_0607 and Synpcc7942_0608 are separated by 48 nucleotides Synpcc7942_0608 and ndhD4 are separated by 83 nucleotides
Synpcc7942_0606: aat - leucyl/phenylalanyl-tRNA--protein transferase, at 594,687 to 595,367
aat
Synpcc7942_0607: Synpcc7942_0607 - hypothetical protein, at 595,381 to 595,707
_0607
Synpcc7942_0608: Synpcc7942_0608 - hypothetical protein, at 595,756 to 596,298
_0608
Synpcc7942_0609: ndhD4 - proton-translocating NADH-quinone oxidoreductase, chain M, at 596,382 to 597,872
ndhD4
Position (kb) 595
596
597 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 594.793 kb on + strand, within aat at 594.801 kb on - strand, within aat at 594.887 kb on - strand, within aat at 595.026 kb on - strand, within aat at 595.031 kb on - strand, within aat at 595.063 kb on - strand, within aat at 595.095 kb on - strand, within aat at 595.152 kb on - strand, within aat at 595.242 kb on + strand, within aat at 595.252 kb on + strand, within aat at 595.255 kb on - strand, within aat at 595.260 kb on - strand, within aat at 595.270 kb on + strand, within aat at 595.403 kb on - strand at 595.458 kb on + strand, within Synpcc7942_0607 at 595.458 kb on + strand, within Synpcc7942_0607 at 595.466 kb on - strand, within Synpcc7942_0607 at 595.471 kb on + strand, within Synpcc7942_0607 at 595.476 kb on - strand, within Synpcc7942_0607 at 595.479 kb on - strand, within Synpcc7942_0607 at 595.537 kb on + strand, within Synpcc7942_0607 at 595.558 kb on + strand, within Synpcc7942_0607 at 595.599 kb on - strand, within Synpcc7942_0607 at 595.603 kb on - strand, within Synpcc7942_0607 at 595.689 kb on - strand at 595.695 kb on + strand at 595.734 kb on - strand at 595.762 kb on + strand at 595.786 kb on - strand at 595.791 kb on - strand at 595.805 kb on - strand at 595.827 kb on + strand, within Synpcc7942_0608 at 595.859 kb on + strand, within Synpcc7942_0608 at 595.973 kb on + strand, within Synpcc7942_0608 at 595.990 kb on - strand, within Synpcc7942_0608 at 595.990 kb on - strand, within Synpcc7942_0608 at 596.012 kb on + strand, within Synpcc7942_0608 at 596.039 kb on - strand, within Synpcc7942_0608 at 596.052 kb on - strand, within Synpcc7942_0608 at 596.103 kb on + strand, within Synpcc7942_0608 at 596.103 kb on + strand, within Synpcc7942_0608 at 596.103 kb on + strand, within Synpcc7942_0608 at 596.118 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.126 kb on - strand, within Synpcc7942_0608 at 596.130 kb on + strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.180 kb on + strand, within Synpcc7942_0608 at 596.188 kb on - strand at 596.199 kb on + strand, within Synpcc7942_0608 at 596.207 kb on + strand, within Synpcc7942_0608 at 596.207 kb on - strand, within Synpcc7942_0608 at 596.212 kb on - strand, within Synpcc7942_0608 at 596.213 kb on - strand, within Synpcc7942_0608 at 596.213 kb on - strand, within Synpcc7942_0608 at 596.288 kb on - strand at 596.313 kb on + strand at 596.320 kb on + strand at 596.320 kb on + strand at 596.320 kb on + strand at 596.320 kb on + strand at 596.320 kb on + strand at 596.328 kb on - strand at 596.328 kb on - strand at 596.328 kb on - strand at 596.328 kb on - strand at 596.332 kb on + strand at 596.332 kb on + strand at 596.366 kb on - strand at 596.366 kb on - strand at 596.387 kb on - strand at 596.437 kb on + strand at 596.445 kb on - strand at 596.445 kb on - strand at 596.452 kb on + strand at 596.461 kb on + strand at 596.570 kb on + strand, within ndhD4 at 596.570 kb on + strand, within ndhD4 at 596.578 kb on - strand, within ndhD4 at 596.578 kb on - strand, within ndhD4 at 596.578 kb on - strand, within ndhD4 at 596.592 kb on - strand, within ndhD4 at 596.592 kb on - strand, within ndhD4 at 596.625 kb on - strand at 596.638 kb on + strand, within ndhD4 at 596.647 kb on - strand, within ndhD4 at 596.647 kb on - strand, within ndhD4 at 596.648 kb on + strand, within ndhD4 at 596.649 kb on + strand, within ndhD4 at 596.649 kb on + strand, within ndhD4 at 596.649 kb on + strand, within ndhD4 at 596.683 kb on + strand, within ndhD4 at 596.705 kb on + strand, within ndhD4 at 596.708 kb on - strand, within ndhD4 at 596.713 kb on - strand, within ndhD4 at 596.741 kb on + strand, within ndhD4 at 596.749 kb on - strand, within ndhD4 at 596.749 kb on - strand, within ndhD4 at 596.764 kb on + strand, within ndhD4 at 596.772 kb on - strand, within ndhD4 at 596.772 kb on - strand, within ndhD4 at 596.772 kb on - strand, within ndhD4 at 596.777 kb on - strand, within ndhD4 at 596.807 kb on + strand, within ndhD4 at 596.888 kb on - strand, within ndhD4 at 596.888 kb on - strand, within ndhD4 at 596.890 kb on - strand, within ndhD4 at 596.975 kb on + strand, within ndhD4 at 596.981 kb on - strand, within ndhD4 at 596.991 kb on - strand, within ndhD4 at 597.019 kb on - strand, within ndhD4 at 597.104 kb on - strand, within ndhD4 at 597.110 kb on + strand, within ndhD4 at 597.144 kb on + strand, within ndhD4 at 597.185 kb on - strand, within ndhD4 at 597.228 kb on + strand, within ndhD4 at 597.228 kb on + strand, within ndhD4 at 597.236 kb on - strand, within ndhD4 at 597.238 kb on - strand at 597.242 kb on + strand, within ndhD4
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 594,793 + aat Synpcc7942_0606 0.16 +0.3 594,801 - aat Synpcc7942_0606 0.17 -3.4 594,887 - aat Synpcc7942_0606 0.29 +1.2 595,026 - aat Synpcc7942_0606 0.50 -0.3 595,031 - aat Synpcc7942_0606 0.51 -1.6 595,063 - aat Synpcc7942_0606 0.55 -3.1 595,095 - aat Synpcc7942_0606 0.60 +0.6 595,152 - aat Synpcc7942_0606 0.68 -2.3 595,242 + aat Synpcc7942_0606 0.81 +1.8 595,252 + aat Synpcc7942_0606 0.83 +0.6 595,255 - aat Synpcc7942_0606 0.83 -0.2 595,260 - aat Synpcc7942_0606 0.84 +0.3 595,270 + aat Synpcc7942_0606 0.86 +2.4 595,403 - +1.1 595,458 + Synpcc7942_0607 0.24 +1.5 595,458 + Synpcc7942_0607 0.24 +2.3 595,466 - Synpcc7942_0607 0.26 -1.8 595,471 + Synpcc7942_0607 0.28 -1.8 595,476 - Synpcc7942_0607 0.29 -1.5 595,479 - Synpcc7942_0607 0.30 +0.0 595,537 + Synpcc7942_0607 0.48 -1.5 595,558 + Synpcc7942_0607 0.54 +0.6 595,599 - Synpcc7942_0607 0.67 +0.9 595,603 - Synpcc7942_0607 0.68 +0.5 595,689 - -0.0 595,695 + -1.8 595,734 - +0.4 595,762 + -1.4 595,786 - -1.7 595,791 - +1.6 595,805 - -3.1 595,827 + Synpcc7942_0608 0.13 -1.0 595,859 + Synpcc7942_0608 0.19 -2.2 595,973 + Synpcc7942_0608 0.40 +0.5 595,990 - Synpcc7942_0608 0.43 +3.4 595,990 - Synpcc7942_0608 0.43 +0.7 596,012 + Synpcc7942_0608 0.47 +4.1 596,039 - Synpcc7942_0608 0.52 -0.9 596,052 - Synpcc7942_0608 0.55 -0.4 596,103 + Synpcc7942_0608 0.64 -1.1 596,103 + Synpcc7942_0608 0.64 -0.9 596,103 + Synpcc7942_0608 0.64 +0.2 596,118 + Synpcc7942_0608 0.67 +2.4 596,125 + Synpcc7942_0608 0.68 -1.9 596,125 + Synpcc7942_0608 0.68 -0.3 596,125 + Synpcc7942_0608 0.68 +0.7 596,125 + Synpcc7942_0608 0.68 -1.3 596,125 + Synpcc7942_0608 0.68 -2.4 596,125 + Synpcc7942_0608 0.68 +0.6 596,126 - Synpcc7942_0608 0.68 +1.1 596,130 + Synpcc7942_0608 0.69 -0.2 596,133 - Synpcc7942_0608 0.69 -1.1 596,133 - Synpcc7942_0608 0.69 +0.9 596,133 - Synpcc7942_0608 0.69 +4.0 596,133 - Synpcc7942_0608 0.69 -4.1 596,133 - Synpcc7942_0608 0.69 +2.1 596,133 - Synpcc7942_0608 0.69 +1.8 596,133 - Synpcc7942_0608 0.69 -3.2 596,180 + Synpcc7942_0608 0.78 +2.5 596,188 - +3.1 596,199 + Synpcc7942_0608 0.82 +3.4 596,207 + Synpcc7942_0608 0.83 -0.1 596,207 - Synpcc7942_0608 0.83 +4.1 596,212 - Synpcc7942_0608 0.84 +2.5 596,213 - Synpcc7942_0608 0.84 -0.2 596,213 - Synpcc7942_0608 0.84 +2.2 596,288 - +0.3 596,313 + -2.7 596,320 + +2.3 596,320 + +1.7 596,320 + +0.3 596,320 + +1.8 596,320 + -1.0 596,328 - +0.8 596,328 - +0.9 596,328 - +1.2 596,328 - +1.5 596,332 + -1.3 596,332 + -0.9 596,366 - -2.9 596,366 - -1.8 596,387 - +1.8 596,437 + +2.3 596,445 - -2.8 596,445 - -1.5 596,452 + +0.0 596,461 + -3.4 596,570 + ndhD4 Synpcc7942_0609 0.13 -2.9 596,570 + ndhD4 Synpcc7942_0609 0.13 -1.8 596,578 - ndhD4 Synpcc7942_0609 0.13 -0.5 596,578 - ndhD4 Synpcc7942_0609 0.13 -1.1 596,578 - ndhD4 Synpcc7942_0609 0.13 +1.3 596,592 - ndhD4 Synpcc7942_0609 0.14 +1.0 596,592 - ndhD4 Synpcc7942_0609 0.14 +0.9 596,625 - +1.0 596,638 + ndhD4 Synpcc7942_0609 0.17 -0.3 596,647 - ndhD4 Synpcc7942_0609 0.18 +0.3 596,647 - ndhD4 Synpcc7942_0609 0.18 +1.9 596,648 + ndhD4 Synpcc7942_0609 0.18 +0.3 596,649 + ndhD4 Synpcc7942_0609 0.18 +0.3 596,649 + ndhD4 Synpcc7942_0609 0.18 -2.6 596,649 + ndhD4 Synpcc7942_0609 0.18 +0.8 596,683 + ndhD4 Synpcc7942_0609 0.20 -1.2 596,705 + ndhD4 Synpcc7942_0609 0.22 -0.6 596,708 - ndhD4 Synpcc7942_0609 0.22 -0.9 596,713 - ndhD4 Synpcc7942_0609 0.22 -0.8 596,741 + ndhD4 Synpcc7942_0609 0.24 +0.5 596,749 - ndhD4 Synpcc7942_0609 0.25 -0.8 596,749 - ndhD4 Synpcc7942_0609 0.25 -4.1 596,764 + ndhD4 Synpcc7942_0609 0.26 +0.1 596,772 - ndhD4 Synpcc7942_0609 0.26 -1.6 596,772 - ndhD4 Synpcc7942_0609 0.26 -2.0 596,772 - ndhD4 Synpcc7942_0609 0.26 -2.3 596,777 - ndhD4 Synpcc7942_0609 0.26 +1.0 596,807 + ndhD4 Synpcc7942_0609 0.29 -1.5 596,888 - ndhD4 Synpcc7942_0609 0.34 +1.0 596,888 - ndhD4 Synpcc7942_0609 0.34 -0.8 596,890 - ndhD4 Synpcc7942_0609 0.34 -1.1 596,975 + ndhD4 Synpcc7942_0609 0.40 -1.3 596,981 - ndhD4 Synpcc7942_0609 0.40 -2.7 596,991 - ndhD4 Synpcc7942_0609 0.41 +0.7 597,019 - ndhD4 Synpcc7942_0609 0.43 -2.6 597,104 - ndhD4 Synpcc7942_0609 0.48 +1.0 597,110 + ndhD4 Synpcc7942_0609 0.49 -1.5 597,144 + ndhD4 Synpcc7942_0609 0.51 +2.4 597,185 - ndhD4 Synpcc7942_0609 0.54 +1.5 597,228 + ndhD4 Synpcc7942_0609 0.57 +0.4 597,228 + ndhD4 Synpcc7942_0609 0.57 -0.1 597,236 - ndhD4 Synpcc7942_0609 0.57 +1.5 597,238 - +1.3 597,242 + ndhD4 Synpcc7942_0609 0.58 +0.9
Or see this region's nucleotide sequence