Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chlI and rpiA are separated by 136 nucleotides rpiA and Synpcc7942_0585 are separated by 88 nucleotides Synpcc7942_0585 and Synpcc7942_0586 are separated by 150 nucleotides
Synpcc7942_0583: chlI - protoporphyrin IX magnesium-chelatase, at 564,123 to 565,217
chlI
Synpcc7942_0584: rpiA - ribose-5-phosphate isomerase A, at 565,354 to 566,079
rpiA
Synpcc7942_0585: Synpcc7942_0585 - PDZ/DHR/GLGF, at 566,168 to 567,388
_0585
Synpcc7942_0586: Synpcc7942_0586 - hypothetical protein, at 567,539 to 568,279
_0586
Position (kb) 566
567
568 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 565.190 kb on + strand at 565.193 kb on - strand at 565.233 kb on + strand at 565.242 kb on - strand at 565.258 kb on - strand at 565.265 kb on + strand at 565.296 kb on - strand at 565.310 kb on + strand at 565.345 kb on + strand at 565.352 kb on - strand at 566.095 kb on - strand at 566.095 kb on - strand at 566.145 kb on + strand at 566.183 kb on - strand at 566.242 kb on - strand at 566.271 kb on - strand at 566.271 kb on - strand at 566.299 kb on + strand, within Synpcc7942_0585 at 566.314 kb on - strand, within Synpcc7942_0585 at 566.321 kb on + strand, within Synpcc7942_0585 at 566.329 kb on - strand, within Synpcc7942_0585 at 566.353 kb on - strand, within Synpcc7942_0585 at 566.366 kb on + strand, within Synpcc7942_0585 at 566.389 kb on - strand, within Synpcc7942_0585 at 566.406 kb on - strand, within Synpcc7942_0585 at 566.421 kb on - strand, within Synpcc7942_0585 at 566.430 kb on + strand, within Synpcc7942_0585 at 566.463 kb on - strand, within Synpcc7942_0585 at 566.470 kb on + strand, within Synpcc7942_0585 at 566.484 kb on - strand, within Synpcc7942_0585 at 566.489 kb on + strand, within Synpcc7942_0585 at 566.489 kb on + strand, within Synpcc7942_0585 at 566.489 kb on - strand, within Synpcc7942_0585 at 566.489 kb on - strand, within Synpcc7942_0585 at 566.497 kb on - strand, within Synpcc7942_0585 at 566.497 kb on - strand, within Synpcc7942_0585 at 566.497 kb on - strand, within Synpcc7942_0585 at 566.619 kb on + strand, within Synpcc7942_0585 at 566.627 kb on - strand, within Synpcc7942_0585 at 566.671 kb on + strand, within Synpcc7942_0585 at 566.671 kb on + strand, within Synpcc7942_0585 at 566.671 kb on + strand, within Synpcc7942_0585 at 566.679 kb on - strand, within Synpcc7942_0585 at 566.679 kb on - strand, within Synpcc7942_0585 at 566.719 kb on + strand, within Synpcc7942_0585 at 566.727 kb on - strand, within Synpcc7942_0585 at 566.753 kb on + strand, within Synpcc7942_0585 at 566.753 kb on + strand, within Synpcc7942_0585 at 566.753 kb on + strand, within Synpcc7942_0585 at 566.755 kb on + strand, within Synpcc7942_0585 at 566.759 kb on - strand, within Synpcc7942_0585 at 566.801 kb on - strand, within Synpcc7942_0585 at 566.801 kb on - strand, within Synpcc7942_0585 at 566.840 kb on - strand, within Synpcc7942_0585 at 566.855 kb on - strand, within Synpcc7942_0585 at 566.870 kb on + strand, within Synpcc7942_0585 at 566.878 kb on - strand, within Synpcc7942_0585 at 566.878 kb on - strand, within Synpcc7942_0585 at 566.887 kb on + strand, within Synpcc7942_0585 at 566.887 kb on + strand, within Synpcc7942_0585 at 566.887 kb on + strand, within Synpcc7942_0585 at 566.887 kb on + strand, within Synpcc7942_0585 at 566.887 kb on + strand, within Synpcc7942_0585 at 566.973 kb on - strand, within Synpcc7942_0585 at 567.124 kb on + strand, within Synpcc7942_0585 at 567.165 kb on - strand, within Synpcc7942_0585 at 567.227 kb on - strand, within Synpcc7942_0585 at 567.231 kb on + strand, within Synpcc7942_0585 at 567.248 kb on + strand, within Synpcc7942_0585 at 567.256 kb on - strand, within Synpcc7942_0585 at 567.256 kb on - strand, within Synpcc7942_0585 at 567.291 kb on - strand at 567.367 kb on + strand at 567.375 kb on - strand at 567.383 kb on + strand at 567.393 kb on + strand at 567.401 kb on - strand at 567.420 kb on + strand at 567.428 kb on - strand at 567.435 kb on - strand at 567.445 kb on - strand at 567.457 kb on + strand at 567.471 kb on + strand at 567.477 kb on - strand at 567.479 kb on - strand at 567.486 kb on - strand at 567.498 kb on - strand at 567.504 kb on - strand at 567.517 kb on - strand at 567.519 kb on + strand at 567.527 kb on - strand at 567.527 kb on - strand at 567.533 kb on - strand at 567.574 kb on + strand at 567.612 kb on + strand at 567.615 kb on - strand at 567.634 kb on + strand, within Synpcc7942_0586 at 567.651 kb on - strand, within Synpcc7942_0586 at 567.651 kb on - strand, within Synpcc7942_0586 at 567.671 kb on + strand at 567.763 kb on + strand, within Synpcc7942_0586 at 567.772 kb on + strand, within Synpcc7942_0586 at 567.772 kb on + strand, within Synpcc7942_0586 at 567.792 kb on - strand, within Synpcc7942_0586 at 567.826 kb on - strand, within Synpcc7942_0586 at 567.859 kb on - strand, within Synpcc7942_0586 at 567.859 kb on - strand, within Synpcc7942_0586 at 567.863 kb on + strand, within Synpcc7942_0586 at 567.870 kb on + strand, within Synpcc7942_0586 at 567.878 kb on - strand, within Synpcc7942_0586 at 567.878 kb on - strand, within Synpcc7942_0586 at 567.880 kb on + strand, within Synpcc7942_0586 at 567.935 kb on + strand, within Synpcc7942_0586 at 567.962 kb on + strand, within Synpcc7942_0586 at 567.962 kb on + strand, within Synpcc7942_0586 at 567.967 kb on + strand, within Synpcc7942_0586 at 567.967 kb on + strand, within Synpcc7942_0586 at 567.970 kb on - strand, within Synpcc7942_0586 at 567.979 kb on - strand, within Synpcc7942_0586 at 567.980 kb on - strand, within Synpcc7942_0586 at 568.023 kb on + strand, within Synpcc7942_0586 at 568.023 kb on + strand, within Synpcc7942_0586 at 568.031 kb on - strand, within Synpcc7942_0586 at 568.031 kb on - strand, within Synpcc7942_0586 at 568.041 kb on - strand, within Synpcc7942_0586 at 568.057 kb on + strand, within Synpcc7942_0586 at 568.067 kb on + strand, within Synpcc7942_0586 at 568.067 kb on + strand, within Synpcc7942_0586 at 568.067 kb on + strand, within Synpcc7942_0586 at 568.075 kb on - strand, within Synpcc7942_0586 at 568.075 kb on - strand, within Synpcc7942_0586 at 568.147 kb on - strand, within Synpcc7942_0586 at 568.200 kb on - strand, within Synpcc7942_0586 at 568.219 kb on - strand at 568.282 kb on - strand at 568.303 kb on - strand at 568.303 kb on - strand at 568.303 kb on - strand at 568.306 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 565,190 + -2.2 565,193 - -1.7 565,233 + -1.3 565,242 - +1.1 565,258 - +0.0 565,265 + -0.3 565,296 - -1.6 565,310 + +1.5 565,345 + -2.8 565,352 - +0.4 566,095 - -1.6 566,095 - +1.2 566,145 + +1.1 566,183 - -1.2 566,242 - -2.2 566,271 - -1.6 566,271 - -2.2 566,299 + Synpcc7942_0585 0.11 -3.1 566,314 - Synpcc7942_0585 0.12 -1.6 566,321 + Synpcc7942_0585 0.13 -3.2 566,329 - Synpcc7942_0585 0.13 -2.6 566,353 - Synpcc7942_0585 0.15 -2.6 566,366 + Synpcc7942_0585 0.16 -1.2 566,389 - Synpcc7942_0585 0.18 +0.7 566,406 - Synpcc7942_0585 0.19 -2.2 566,421 - Synpcc7942_0585 0.21 -3.0 566,430 + Synpcc7942_0585 0.21 -2.4 566,463 - Synpcc7942_0585 0.24 -2.5 566,470 + Synpcc7942_0585 0.25 -4.0 566,484 - Synpcc7942_0585 0.26 -3.2 566,489 + Synpcc7942_0585 0.26 -0.7 566,489 + Synpcc7942_0585 0.26 -0.3 566,489 - Synpcc7942_0585 0.26 -2.7 566,489 - Synpcc7942_0585 0.26 -3.1 566,497 - Synpcc7942_0585 0.27 -1.3 566,497 - Synpcc7942_0585 0.27 -2.8 566,497 - Synpcc7942_0585 0.27 -4.0 566,619 + Synpcc7942_0585 0.37 -1.2 566,627 - Synpcc7942_0585 0.38 -0.3 566,671 + Synpcc7942_0585 0.41 +0.6 566,671 + Synpcc7942_0585 0.41 -2.6 566,671 + Synpcc7942_0585 0.41 -2.3 566,679 - Synpcc7942_0585 0.42 -2.4 566,679 - Synpcc7942_0585 0.42 -2.2 566,719 + Synpcc7942_0585 0.45 -0.6 566,727 - Synpcc7942_0585 0.46 -2.0 566,753 + Synpcc7942_0585 0.48 -3.7 566,753 + Synpcc7942_0585 0.48 +0.4 566,753 + Synpcc7942_0585 0.48 -2.9 566,755 + Synpcc7942_0585 0.48 -2.4 566,759 - Synpcc7942_0585 0.48 -4.9 566,801 - Synpcc7942_0585 0.52 -0.6 566,801 - Synpcc7942_0585 0.52 -0.8 566,840 - Synpcc7942_0585 0.55 -2.4 566,855 - Synpcc7942_0585 0.56 -1.4 566,870 + Synpcc7942_0585 0.57 -1.4 566,878 - Synpcc7942_0585 0.58 -1.3 566,878 - Synpcc7942_0585 0.58 -3.9 566,887 + Synpcc7942_0585 0.59 -2.9 566,887 + Synpcc7942_0585 0.59 -2.7 566,887 + Synpcc7942_0585 0.59 -3.2 566,887 + Synpcc7942_0585 0.59 -0.8 566,887 + Synpcc7942_0585 0.59 -1.4 566,973 - Synpcc7942_0585 0.66 -2.1 567,124 + Synpcc7942_0585 0.78 -2.1 567,165 - Synpcc7942_0585 0.82 -4.1 567,227 - Synpcc7942_0585 0.87 -2.3 567,231 + Synpcc7942_0585 0.87 -2.7 567,248 + Synpcc7942_0585 0.88 -1.6 567,256 - Synpcc7942_0585 0.89 -0.8 567,256 - Synpcc7942_0585 0.89 -2.2 567,291 - -2.0 567,367 + -3.5 567,375 - -3.8 567,383 + -2.8 567,393 + -3.7 567,401 - -0.8 567,420 + +0.6 567,428 - -2.9 567,435 - -3.6 567,445 - -2.3 567,457 + -2.3 567,471 + -4.1 567,477 - -0.6 567,479 - -3.4 567,486 - -2.1 567,498 - -2.6 567,504 - -1.0 567,517 - +0.0 567,519 + -0.1 567,527 - +1.5 567,527 - -2.3 567,533 - +0.8 567,574 + +0.9 567,612 + -0.5 567,615 - +1.0 567,634 + Synpcc7942_0586 0.13 +0.5 567,651 - Synpcc7942_0586 0.15 -0.8 567,651 - Synpcc7942_0586 0.15 +2.5 567,671 + +0.0 567,763 + Synpcc7942_0586 0.30 +1.9 567,772 + Synpcc7942_0586 0.31 +2.8 567,772 + Synpcc7942_0586 0.31 +1.5 567,792 - Synpcc7942_0586 0.34 -1.9 567,826 - Synpcc7942_0586 0.39 -0.7 567,859 - Synpcc7942_0586 0.43 +0.8 567,859 - Synpcc7942_0586 0.43 -1.4 567,863 + Synpcc7942_0586 0.44 -1.4 567,870 + Synpcc7942_0586 0.45 -0.6 567,878 - Synpcc7942_0586 0.46 -0.4 567,878 - Synpcc7942_0586 0.46 -0.8 567,880 + Synpcc7942_0586 0.46 +1.2 567,935 + Synpcc7942_0586 0.53 +0.8 567,962 + Synpcc7942_0586 0.57 +3.0 567,962 + Synpcc7942_0586 0.57 -1.7 567,967 + Synpcc7942_0586 0.58 +1.9 567,967 + Synpcc7942_0586 0.58 +1.9 567,970 - Synpcc7942_0586 0.58 -0.4 567,979 - Synpcc7942_0586 0.59 +0.6 567,980 - Synpcc7942_0586 0.60 +3.2 568,023 + Synpcc7942_0586 0.65 +2.5 568,023 + Synpcc7942_0586 0.65 +2.0 568,031 - Synpcc7942_0586 0.66 -0.8 568,031 - Synpcc7942_0586 0.66 +0.1 568,041 - Synpcc7942_0586 0.68 +1.2 568,057 + Synpcc7942_0586 0.70 +3.5 568,067 + Synpcc7942_0586 0.71 +1.5 568,067 + Synpcc7942_0586 0.71 -2.6 568,067 + Synpcc7942_0586 0.71 -4.6 568,075 - Synpcc7942_0586 0.72 -1.3 568,075 - Synpcc7942_0586 0.72 -0.8 568,147 - Synpcc7942_0586 0.82 +1.6 568,200 - Synpcc7942_0586 0.89 -0.8 568,219 - -1.2 568,282 - -3.0 568,303 - +0.9 568,303 - +1.0 568,303 - +0.1 568,306 + +0.1
Or see this region's nucleotide sequence