Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0573 and Synpcc7942_0574 are separated by 78 nucleotides Synpcc7942_0574 and Synpcc7942_0575 overlap by 29 nucleotides Synpcc7942_0575 and thiG are separated by 222 nucleotides
Synpcc7942_0573: Synpcc7942_0573 - hypothetical protein, at 555,297 to 555,533
_0573
Synpcc7942_0574: Synpcc7942_0574 - hypothetical protein, at 555,612 to 556,442
_0574
Synpcc7942_0575: Synpcc7942_0575 - hypothetical protein, at 556,414 to 556,977
_0575
Synpcc7942_0576: thiG - thiazole synthase, at 557,200 to 558,060
thiG
Position (kb) 556
557 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 555.419 kb on - strand, within Synpcc7942_0573 at 555.477 kb on + strand, within Synpcc7942_0573 at 555.491 kb on - strand, within Synpcc7942_0573 at 555.510 kb on - strand at 555.518 kb on + strand at 555.553 kb on + strand at 555.558 kb on + strand at 555.602 kb on - strand at 555.633 kb on - strand at 555.648 kb on + strand at 555.664 kb on + strand at 555.664 kb on + strand at 555.664 kb on + strand at 555.677 kb on + strand at 555.681 kb on - strand at 555.696 kb on - strand, within Synpcc7942_0574 at 555.721 kb on - strand, within Synpcc7942_0574 at 555.733 kb on + strand, within Synpcc7942_0574 at 555.749 kb on + strand, within Synpcc7942_0574 at 555.820 kb on + strand, within Synpcc7942_0574 at 555.825 kb on + strand, within Synpcc7942_0574 at 555.895 kb on - strand, within Synpcc7942_0574 at 555.897 kb on + strand, within Synpcc7942_0574 at 555.897 kb on + strand, within Synpcc7942_0574 at 555.897 kb on + strand, within Synpcc7942_0574 at 555.897 kb on + strand, within Synpcc7942_0574 at 555.897 kb on + strand, within Synpcc7942_0574 at 555.905 kb on - strand, within Synpcc7942_0574 at 555.913 kb on + strand, within Synpcc7942_0574 at 555.915 kb on + strand, within Synpcc7942_0574 at 555.921 kb on - strand, within Synpcc7942_0574 at 555.921 kb on - strand, within Synpcc7942_0574 at 555.921 kb on - strand, within Synpcc7942_0574 at 555.921 kb on - strand, within Synpcc7942_0574 at 555.987 kb on + strand, within Synpcc7942_0574 at 555.987 kb on + strand, within Synpcc7942_0574 at 555.997 kb on + strand, within Synpcc7942_0574 at 555.998 kb on - strand, within Synpcc7942_0574 at 556.005 kb on - strand, within Synpcc7942_0574 at 556.018 kb on - strand, within Synpcc7942_0574 at 556.023 kb on - strand, within Synpcc7942_0574 at 556.027 kb on + strand, within Synpcc7942_0574 at 556.037 kb on - strand, within Synpcc7942_0574 at 556.037 kb on - strand, within Synpcc7942_0574 at 556.037 kb on - strand, within Synpcc7942_0574 at 556.149 kb on + strand, within Synpcc7942_0574 at 556.153 kb on - strand, within Synpcc7942_0574 at 556.162 kb on - strand, within Synpcc7942_0574 at 556.162 kb on - strand, within Synpcc7942_0574 at 556.181 kb on - strand, within Synpcc7942_0574 at 556.233 kb on + strand, within Synpcc7942_0574 at 556.241 kb on - strand, within Synpcc7942_0574 at 556.253 kb on - strand, within Synpcc7942_0574 at 556.257 kb on + strand, within Synpcc7942_0574 at 556.286 kb on - strand, within Synpcc7942_0574 at 556.289 kb on - strand, within Synpcc7942_0574 at 556.311 kb on + strand, within Synpcc7942_0574 at 556.350 kb on - strand, within Synpcc7942_0574 at 556.390 kb on - strand at 556.395 kb on - strand at 556.412 kb on + strand at 556.460 kb on - strand at 556.462 kb on - strand at 556.467 kb on - strand at 556.482 kb on - strand, within Synpcc7942_0575 at 556.482 kb on - strand, within Synpcc7942_0575 at 556.484 kb on + strand, within Synpcc7942_0575 at 556.492 kb on - strand, within Synpcc7942_0575 at 556.527 kb on + strand, within Synpcc7942_0575 at 556.638 kb on + strand, within Synpcc7942_0575 at 556.646 kb on - strand, within Synpcc7942_0575 at 556.656 kb on - strand, within Synpcc7942_0575 at 556.666 kb on - strand, within Synpcc7942_0575 at 556.671 kb on - strand, within Synpcc7942_0575 at 556.676 kb on + strand, within Synpcc7942_0575 at 556.679 kb on - strand, within Synpcc7942_0575 at 556.681 kb on + strand, within Synpcc7942_0575 at 556.684 kb on - strand, within Synpcc7942_0575 at 556.689 kb on - strand, within Synpcc7942_0575 at 556.716 kb on + strand, within Synpcc7942_0575 at 556.716 kb on + strand, within Synpcc7942_0575 at 556.724 kb on - strand, within Synpcc7942_0575 at 556.724 kb on - strand, within Synpcc7942_0575 at 556.724 kb on - strand, within Synpcc7942_0575 at 556.724 kb on - strand, within Synpcc7942_0575 at 556.724 kb on - strand, within Synpcc7942_0575 at 556.725 kb on - strand, within Synpcc7942_0575 at 556.735 kb on + strand, within Synpcc7942_0575 at 556.738 kb on - strand, within Synpcc7942_0575 at 556.743 kb on - strand, within Synpcc7942_0575 at 556.743 kb on - strand, within Synpcc7942_0575 at 556.743 kb on - strand, within Synpcc7942_0575 at 556.755 kb on - strand, within Synpcc7942_0575 at 556.760 kb on + strand, within Synpcc7942_0575 at 556.799 kb on + strand, within Synpcc7942_0575 at 556.804 kb on + strand, within Synpcc7942_0575 at 556.816 kb on + strand, within Synpcc7942_0575 at 556.825 kb on - strand, within Synpcc7942_0575 at 556.825 kb on - strand, within Synpcc7942_0575 at 556.921 kb on + strand at 556.974 kb on + strand at 556.985 kb on + strand at 556.985 kb on + strand at 557.031 kb on + strand at 557.031 kb on + strand at 557.031 kb on + strand at 557.039 kb on - strand at 557.039 kb on - strand at 557.039 kb on - strand at 557.044 kb on - strand at 557.044 kb on - strand at 557.044 kb on - strand at 557.059 kb on - strand at 557.081 kb on - strand at 557.091 kb on + strand at 557.099 kb on - strand at 557.104 kb on - strand at 557.189 kb on - strand at 557.198 kb on - strand at 557.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 555,419 - Synpcc7942_0573 0.51 -0.3 555,477 + Synpcc7942_0573 0.76 +0.2 555,491 - Synpcc7942_0573 0.82 +0.7 555,510 - +0.3 555,518 + +0.8 555,553 + -1.0 555,558 + +2.8 555,602 - +3.6 555,633 - +2.7 555,648 + -0.9 555,664 + -0.7 555,664 + -0.3 555,664 + -1.6 555,677 + -0.4 555,681 - -1.3 555,696 - Synpcc7942_0574 0.10 +2.1 555,721 - Synpcc7942_0574 0.13 +1.3 555,733 + Synpcc7942_0574 0.15 -0.7 555,749 + Synpcc7942_0574 0.16 +2.0 555,820 + Synpcc7942_0574 0.25 -0.1 555,825 + Synpcc7942_0574 0.26 +0.5 555,895 - Synpcc7942_0574 0.34 -2.7 555,897 + Synpcc7942_0574 0.34 -2.6 555,897 + Synpcc7942_0574 0.34 -2.0 555,897 + Synpcc7942_0574 0.34 +1.2 555,897 + Synpcc7942_0574 0.34 +1.6 555,897 + Synpcc7942_0574 0.34 +1.1 555,905 - Synpcc7942_0574 0.35 -1.7 555,913 + Synpcc7942_0574 0.36 -0.3 555,915 + Synpcc7942_0574 0.36 -1.2 555,921 - Synpcc7942_0574 0.37 +0.6 555,921 - Synpcc7942_0574 0.37 +1.4 555,921 - Synpcc7942_0574 0.37 -0.9 555,921 - Synpcc7942_0574 0.37 -1.6 555,987 + Synpcc7942_0574 0.45 +1.1 555,987 + Synpcc7942_0574 0.45 -1.1 555,997 + Synpcc7942_0574 0.46 -2.0 555,998 - Synpcc7942_0574 0.46 +3.5 556,005 - Synpcc7942_0574 0.47 +0.5 556,018 - Synpcc7942_0574 0.49 -3.1 556,023 - Synpcc7942_0574 0.49 -2.0 556,027 + Synpcc7942_0574 0.50 +0.5 556,037 - Synpcc7942_0574 0.51 +2.0 556,037 - Synpcc7942_0574 0.51 -0.8 556,037 - Synpcc7942_0574 0.51 -0.6 556,149 + Synpcc7942_0574 0.65 +1.4 556,153 - Synpcc7942_0574 0.65 +0.5 556,162 - Synpcc7942_0574 0.66 -2.7 556,162 - Synpcc7942_0574 0.66 +0.2 556,181 - Synpcc7942_0574 0.68 +1.8 556,233 + Synpcc7942_0574 0.75 +0.7 556,241 - Synpcc7942_0574 0.76 -0.5 556,253 - Synpcc7942_0574 0.77 +1.3 556,257 + Synpcc7942_0574 0.78 +1.3 556,286 - Synpcc7942_0574 0.81 +1.0 556,289 - Synpcc7942_0574 0.81 +0.4 556,311 + Synpcc7942_0574 0.84 -1.0 556,350 - Synpcc7942_0574 0.89 -1.4 556,390 - +3.7 556,395 - -1.7 556,412 + -1.0 556,460 - +3.9 556,462 - +0.0 556,467 - +4.1 556,482 - Synpcc7942_0575 0.12 +1.8 556,482 - Synpcc7942_0575 0.12 +2.6 556,484 + Synpcc7942_0575 0.12 +0.0 556,492 - Synpcc7942_0575 0.14 +3.6 556,527 + Synpcc7942_0575 0.20 +4.9 556,638 + Synpcc7942_0575 0.40 +3.8 556,646 - Synpcc7942_0575 0.41 +0.4 556,656 - Synpcc7942_0575 0.43 +3.8 556,666 - Synpcc7942_0575 0.45 +3.2 556,671 - Synpcc7942_0575 0.46 -1.4 556,676 + Synpcc7942_0575 0.46 +1.1 556,679 - Synpcc7942_0575 0.47 +0.5 556,681 + Synpcc7942_0575 0.47 +0.6 556,684 - Synpcc7942_0575 0.48 +4.9 556,689 - Synpcc7942_0575 0.49 +5.1 556,716 + Synpcc7942_0575 0.54 -0.1 556,716 + Synpcc7942_0575 0.54 -1.7 556,724 - Synpcc7942_0575 0.55 -0.3 556,724 - Synpcc7942_0575 0.55 +0.7 556,724 - Synpcc7942_0575 0.55 +4.3 556,724 - Synpcc7942_0575 0.55 +5.6 556,724 - Synpcc7942_0575 0.55 +2.2 556,725 - Synpcc7942_0575 0.55 -0.4 556,735 + Synpcc7942_0575 0.57 +2.2 556,738 - Synpcc7942_0575 0.57 +3.2 556,743 - Synpcc7942_0575 0.58 +0.7 556,743 - Synpcc7942_0575 0.58 -0.3 556,743 - Synpcc7942_0575 0.58 -2.1 556,755 - Synpcc7942_0575 0.60 +2.7 556,760 + Synpcc7942_0575 0.61 +3.1 556,799 + Synpcc7942_0575 0.68 +3.2 556,804 + Synpcc7942_0575 0.69 +3.7 556,816 + Synpcc7942_0575 0.71 +3.7 556,825 - Synpcc7942_0575 0.73 +1.2 556,825 - Synpcc7942_0575 0.73 -0.3 556,921 + +1.1 556,974 + +1.4 556,985 + +3.9 556,985 + +0.6 557,031 + -1.8 557,031 + -2.3 557,031 + +0.1 557,039 - +0.9 557,039 - -1.2 557,039 - +0.2 557,044 - +0.4 557,044 - -1.3 557,044 - -1.3 557,059 - -1.4 557,081 - -1.3 557,091 + -1.0 557,099 - +1.4 557,104 - -0.4 557,189 - +0.6 557,198 - +0.3 557,226 - +1.6
Or see this region's nucleotide sequence