Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroB and lacF are separated by 79 nucleotides lacF and Synpcc7942_0527 are separated by 104 nucleotides
Synpcc7942_0525: aroB - 3-dehydroquinate synthase, at 509,226 to 510,332
aroB
Synpcc7942_0526: lacF - ABC-type sugar transport systems permease components-like, at 510,412 to 511,293
lacF
Synpcc7942_0527: Synpcc7942_0527 - hypothetical protein, at 511,398 to 512,234
_0527
Position (kb) 510
511
512 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 510.369 kb on - strand at 510.370 kb on + strand at 510.378 kb on - strand at 510.396 kb on - strand at 510.406 kb on - strand at 510.416 kb on - strand at 510.421 kb on - strand at 510.486 kb on + strand at 510.494 kb on - strand at 510.532 kb on + strand, within lacF at 510.588 kb on + strand, within lacF at 510.613 kb on + strand, within lacF at 510.633 kb on - strand, within lacF at 510.670 kb on + strand, within lacF at 510.678 kb on - strand, within lacF at 510.718 kb on - strand, within lacF at 510.718 kb on - strand, within lacF at 510.730 kb on + strand, within lacF at 510.738 kb on - strand, within lacF at 510.786 kb on + strand, within lacF at 510.792 kb on + strand, within lacF at 510.802 kb on + strand, within lacF at 510.802 kb on + strand, within lacF at 510.805 kb on - strand, within lacF at 510.823 kb on - strand, within lacF at 510.825 kb on + strand, within lacF at 510.854 kb on + strand, within lacF at 510.874 kb on + strand, within lacF at 510.874 kb on + strand, within lacF at 510.877 kb on - strand, within lacF at 510.885 kb on + strand, within lacF at 510.893 kb on - strand, within lacF at 510.893 kb on - strand, within lacF at 510.919 kb on - strand, within lacF at 510.922 kb on + strand, within lacF at 510.926 kb on + strand, within lacF at 510.929 kb on - strand, within lacF at 510.936 kb on + strand, within lacF at 510.937 kb on - strand, within lacF at 510.965 kb on + strand, within lacF at 510.973 kb on - strand, within lacF at 510.988 kb on + strand, within lacF at 510.996 kb on - strand, within lacF at 511.018 kb on + strand, within lacF at 511.052 kb on + strand, within lacF at 511.060 kb on - strand, within lacF at 511.127 kb on - strand, within lacF at 511.135 kb on + strand, within lacF at 511.166 kb on - strand, within lacF at 511.203 kb on + strand, within lacF at 511.211 kb on - strand at 511.225 kb on - strand at 511.227 kb on + strand at 511.245 kb on - strand at 511.456 kb on + strand at 511.456 kb on - strand at 511.464 kb on - strand at 511.464 kb on - strand at 511.464 kb on - strand at 511.535 kb on - strand, within Synpcc7942_0527 at 511.653 kb on + strand, within Synpcc7942_0527 at 511.661 kb on - strand, within Synpcc7942_0527 at 511.671 kb on - strand, within Synpcc7942_0527 at 511.721 kb on - strand, within Synpcc7942_0527 at 511.762 kb on + strand, within Synpcc7942_0527 at 511.763 kb on + strand, within Synpcc7942_0527 at 511.770 kb on - strand, within Synpcc7942_0527 at 511.771 kb on - strand, within Synpcc7942_0527 at 511.771 kb on - strand, within Synpcc7942_0527 at 511.946 kb on - strand, within Synpcc7942_0527 at 511.960 kb on + strand, within Synpcc7942_0527 at 511.964 kb on - strand, within Synpcc7942_0527 at 511.968 kb on - strand, within Synpcc7942_0527 at 512.049 kb on - strand, within Synpcc7942_0527 at 512.068 kb on - strand, within Synpcc7942_0527 at 512.085 kb on + strand, within Synpcc7942_0527 at 512.092 kb on + strand, within Synpcc7942_0527 at 512.092 kb on + strand, within Synpcc7942_0527 at 512.095 kb on + strand, within Synpcc7942_0527 at 512.095 kb on + strand, within Synpcc7942_0527 at 512.100 kb on - strand, within Synpcc7942_0527 at 512.127 kb on - strand, within Synpcc7942_0527 at 512.129 kb on - strand, within Synpcc7942_0527 at 512.139 kb on - strand, within Synpcc7942_0527 at 512.144 kb on + strand, within Synpcc7942_0527 at 512.178 kb on - strand at 512.178 kb on - strand at 512.191 kb on + strand at 512.191 kb on + strand at 512.199 kb on - strand at 512.210 kb on - strand at 512.210 kb on - strand at 512.235 kb on + strand at 512.235 kb on + strand at 512.243 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 510,369 - +2.1 510,370 + -0.5 510,378 - +3.4 510,396 - +2.3 510,406 - +1.2 510,416 - +0.5 510,421 - +0.1 510,486 + -1.6 510,494 - -2.5 510,532 + lacF Synpcc7942_0526 0.14 -2.4 510,588 + lacF Synpcc7942_0526 0.20 -1.4 510,613 + lacF Synpcc7942_0526 0.23 -3.1 510,633 - lacF Synpcc7942_0526 0.25 -3.2 510,670 + lacF Synpcc7942_0526 0.29 -1.1 510,678 - lacF Synpcc7942_0526 0.30 -1.4 510,718 - lacF Synpcc7942_0526 0.35 -1.8 510,718 - lacF Synpcc7942_0526 0.35 +1.5 510,730 + lacF Synpcc7942_0526 0.36 -2.4 510,738 - lacF Synpcc7942_0526 0.37 -3.6 510,786 + lacF Synpcc7942_0526 0.42 -3.2 510,792 + lacF Synpcc7942_0526 0.43 -3.9 510,802 + lacF Synpcc7942_0526 0.44 -2.4 510,802 + lacF Synpcc7942_0526 0.44 -3.1 510,805 - lacF Synpcc7942_0526 0.45 -2.0 510,823 - lacF Synpcc7942_0526 0.47 -2.9 510,825 + lacF Synpcc7942_0526 0.47 -2.1 510,854 + lacF Synpcc7942_0526 0.50 -1.9 510,874 + lacF Synpcc7942_0526 0.52 -1.9 510,874 + lacF Synpcc7942_0526 0.52 -0.5 510,877 - lacF Synpcc7942_0526 0.53 -1.9 510,885 + lacF Synpcc7942_0526 0.54 -0.9 510,893 - lacF Synpcc7942_0526 0.55 -1.9 510,893 - lacF Synpcc7942_0526 0.55 -0.3 510,919 - lacF Synpcc7942_0526 0.57 -2.2 510,922 + lacF Synpcc7942_0526 0.58 +1.7 510,926 + lacF Synpcc7942_0526 0.58 -2.9 510,929 - lacF Synpcc7942_0526 0.59 -3.3 510,936 + lacF Synpcc7942_0526 0.59 -2.6 510,937 - lacF Synpcc7942_0526 0.60 -0.3 510,965 + lacF Synpcc7942_0526 0.63 -2.2 510,973 - lacF Synpcc7942_0526 0.64 -2.5 510,988 + lacF Synpcc7942_0526 0.65 -5.3 510,996 - lacF Synpcc7942_0526 0.66 -0.9 511,018 + lacF Synpcc7942_0526 0.69 -4.4 511,052 + lacF Synpcc7942_0526 0.73 -3.0 511,060 - lacF Synpcc7942_0526 0.73 -2.4 511,127 - lacF Synpcc7942_0526 0.81 -0.5 511,135 + lacF Synpcc7942_0526 0.82 -3.8 511,166 - lacF Synpcc7942_0526 0.85 -3.2 511,203 + lacF Synpcc7942_0526 0.90 -1.9 511,211 - -1.5 511,225 - -2.6 511,227 + -4.4 511,245 - -3.6 511,456 + +0.8 511,456 - -0.6 511,464 - +1.0 511,464 - +1.6 511,464 - +0.1 511,535 - Synpcc7942_0527 0.16 +1.3 511,653 + Synpcc7942_0527 0.30 -0.2 511,661 - Synpcc7942_0527 0.31 -0.5 511,671 - Synpcc7942_0527 0.33 -1.5 511,721 - Synpcc7942_0527 0.39 +3.1 511,762 + Synpcc7942_0527 0.43 +0.2 511,763 + Synpcc7942_0527 0.44 +1.0 511,770 - Synpcc7942_0527 0.44 +1.3 511,771 - Synpcc7942_0527 0.45 +0.5 511,771 - Synpcc7942_0527 0.45 +2.9 511,946 - Synpcc7942_0527 0.65 -0.6 511,960 + Synpcc7942_0527 0.67 -2.0 511,964 - Synpcc7942_0527 0.68 -1.6 511,968 - Synpcc7942_0527 0.68 +1.4 512,049 - Synpcc7942_0527 0.78 -1.7 512,068 - Synpcc7942_0527 0.80 -0.5 512,085 + Synpcc7942_0527 0.82 -0.1 512,092 + Synpcc7942_0527 0.83 -2.4 512,092 + Synpcc7942_0527 0.83 +0.3 512,095 + Synpcc7942_0527 0.83 +0.6 512,095 + Synpcc7942_0527 0.83 -0.9 512,100 - Synpcc7942_0527 0.84 -0.7 512,127 - Synpcc7942_0527 0.87 +1.1 512,129 - Synpcc7942_0527 0.87 +0.9 512,139 - Synpcc7942_0527 0.89 -0.0 512,144 + Synpcc7942_0527 0.89 +1.4 512,178 - -1.5 512,178 - -0.9 512,191 + +0.6 512,191 + -2.4 512,199 - +0.6 512,210 - +0.6 512,210 - +0.5 512,235 + -1.4 512,235 + +2.1 512,243 - +3.4
Or see this region's nucleotide sequence