Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0470 and lacG are separated by 137 nucleotides lacG and Synpcc7942_0472 are separated by 82 nucleotides Synpcc7942_0472 and Synpcc7942_0473 are separated by 76 nucleotides
Synpcc7942_0470: Synpcc7942_0470 - hypothetical protein, at 457,211 to 457,744
_0470
Synpcc7942_0471: lacG - ABC-type sugar transport system permease component-like, at 457,882 to 458,712
lacG
Synpcc7942_0472: Synpcc7942_0472 - Exodeoxyribonuclease VII small subunit, at 458,795 to 459,019
_0472
Synpcc7942_0473: Synpcc7942_0473 - NADH:flavin oxidoreductase Old Yellow Enzyme family-like, at 459,096 to 460,202
_0473
Position (kb) 457
458
459 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 456.959 kb on + strand at 456.959 kb on + strand at 456.966 kb on - strand at 456.967 kb on - strand at 456.967 kb on - strand at 456.967 kb on - strand at 456.969 kb on + strand at 456.974 kb on + strand at 456.977 kb on - strand at 456.982 kb on - strand at 456.982 kb on - strand at 456.992 kb on + strand at 457.044 kb on - strand at 457.060 kb on + strand at 457.136 kb on + strand at 457.137 kb on + strand at 457.159 kb on + strand at 457.162 kb on - strand at 457.164 kb on - strand at 457.167 kb on - strand at 457.186 kb on - strand at 457.205 kb on - strand at 457.260 kb on - strand at 457.299 kb on - strand, within Synpcc7942_0470 at 457.304 kb on + strand, within Synpcc7942_0470 at 457.304 kb on + strand, within Synpcc7942_0470 at 457.312 kb on - strand, within Synpcc7942_0470 at 457.312 kb on - strand, within Synpcc7942_0470 at 457.312 kb on - strand, within Synpcc7942_0470 at 457.312 kb on - strand, within Synpcc7942_0470 at 457.312 kb on - strand, within Synpcc7942_0470 at 457.346 kb on + strand, within Synpcc7942_0470 at 457.351 kb on + strand, within Synpcc7942_0470 at 457.385 kb on + strand, within Synpcc7942_0470 at 457.393 kb on - strand, within Synpcc7942_0470 at 457.412 kb on + strand at 457.441 kb on + strand at 457.441 kb on + strand at 457.477 kb on - strand, within Synpcc7942_0470 at 457.479 kb on + strand, within Synpcc7942_0470 at 457.482 kb on - strand, within Synpcc7942_0470 at 457.484 kb on + strand, within Synpcc7942_0470 at 457.490 kb on + strand, within Synpcc7942_0470 at 457.492 kb on - strand, within Synpcc7942_0470 at 457.492 kb on - strand at 457.508 kb on - strand, within Synpcc7942_0470 at 457.518 kb on + strand, within Synpcc7942_0470 at 457.525 kb on - strand, within Synpcc7942_0470 at 457.553 kb on - strand, within Synpcc7942_0470 at 457.572 kb on + strand, within Synpcc7942_0470 at 457.580 kb on - strand, within Synpcc7942_0470 at 457.582 kb on + strand, within Synpcc7942_0470 at 457.598 kb on + strand, within Synpcc7942_0470 at 457.605 kb on - strand, within Synpcc7942_0470 at 457.605 kb on - strand, within Synpcc7942_0470 at 457.608 kb on + strand, within Synpcc7942_0470 at 457.616 kb on - strand, within Synpcc7942_0470 at 457.647 kb on - strand, within Synpcc7942_0470 at 457.655 kb on + strand, within Synpcc7942_0470 at 457.692 kb on - strand at 457.692 kb on - strand at 457.757 kb on - strand at 457.780 kb on - strand at 457.785 kb on + strand at 457.785 kb on + strand at 457.793 kb on - strand at 457.803 kb on - strand at 457.843 kb on + strand at 457.870 kb on + strand at 457.880 kb on - strand at 457.884 kb on + strand at 457.887 kb on - strand at 457.920 kb on - strand at 457.922 kb on - strand at 457.922 kb on - strand at 457.934 kb on + strand at 457.989 kb on - strand, within lacG at 457.989 kb on - strand, within lacG at 458.005 kb on - strand, within lacG at 458.086 kb on + strand, within lacG at 458.094 kb on - strand, within lacG at 458.124 kb on - strand, within lacG at 458.137 kb on + strand, within lacG at 458.145 kb on + strand, within lacG at 458.161 kb on + strand, within lacG at 458.169 kb on - strand, within lacG at 458.169 kb on - strand, within lacG at 458.254 kb on + strand, within lacG at 458.262 kb on + strand, within lacG at 458.264 kb on + strand, within lacG at 458.264 kb on + strand, within lacG at 458.274 kb on - strand, within lacG at 458.279 kb on + strand, within lacG at 458.279 kb on + strand, within lacG at 458.289 kb on + strand, within lacG at 458.294 kb on + strand, within lacG at 458.294 kb on + strand, within lacG at 458.297 kb on - strand, within lacG at 458.302 kb on - strand, within lacG at 458.424 kb on - strand, within lacG at 458.483 kb on - strand, within lacG at 458.490 kb on + strand, within lacG at 458.545 kb on + strand, within lacG at 458.550 kb on + strand, within lacG at 458.551 kb on + strand, within lacG at 458.551 kb on + strand, within lacG at 458.559 kb on - strand, within lacG at 458.572 kb on + strand, within lacG at 458.625 kb on - strand, within lacG at 458.626 kb on - strand, within lacG at 458.667 kb on + strand at 458.667 kb on + strand at 458.667 kb on + strand at 458.667 kb on + strand at 458.675 kb on - strand at 458.706 kb on - strand at 458.707 kb on + strand at 458.714 kb on - strand at 458.752 kb on + strand at 458.767 kb on + strand at 458.772 kb on + strand at 458.803 kb on + strand at 458.803 kb on + strand at 458.811 kb on - strand at 458.861 kb on + strand, within Synpcc7942_0472 at 458.871 kb on + strand, within Synpcc7942_0472 at 458.879 kb on - strand, within Synpcc7942_0472 at 458.881 kb on + strand, within Synpcc7942_0472 at 458.889 kb on - strand, within Synpcc7942_0472 at 458.889 kb on - strand, within Synpcc7942_0472 at 458.974 kb on - strand, within Synpcc7942_0472 at 459.033 kb on + strand at 459.086 kb on + strand at 459.094 kb on - strand at 459.094 kb on - strand at 459.126 kb on + strand at 459.126 kb on + strand at 459.139 kb on + strand at 459.162 kb on + strand at 459.171 kb on + strand at 459.203 kb on - strand at 459.217 kb on + strand, within Synpcc7942_0473 at 459.225 kb on - strand, within Synpcc7942_0473 at 459.245 kb on + strand, within Synpcc7942_0473 at 459.281 kb on + strand, within Synpcc7942_0473 at 459.294 kb on + strand, within Synpcc7942_0473 at 459.297 kb on - strand, within Synpcc7942_0473 at 459.302 kb on - strand, within Synpcc7942_0473 at 459.302 kb on - strand, within Synpcc7942_0473 at 459.302 kb on - strand, within Synpcc7942_0473 at 459.313 kb on + strand, within Synpcc7942_0473 at 459.313 kb on + strand, within Synpcc7942_0473 at 459.315 kb on - strand, within Synpcc7942_0473 at 459.328 kb on + strand, within Synpcc7942_0473 at 459.328 kb on + strand, within Synpcc7942_0473 at 459.330 kb on + strand, within Synpcc7942_0473 at 459.336 kb on - strand, within Synpcc7942_0473 at 459.345 kb on - strand, within Synpcc7942_0473 at 459.346 kb on - strand, within Synpcc7942_0473 at 459.346 kb on - strand, within Synpcc7942_0473 at 459.375 kb on - strand, within Synpcc7942_0473 at 459.401 kb on + strand, within Synpcc7942_0473 at 459.445 kb on + strand, within Synpcc7942_0473 at 459.448 kb on + strand, within Synpcc7942_0473 at 459.455 kb on + strand, within Synpcc7942_0473 at 459.470 kb on - strand, within Synpcc7942_0473 at 459.491 kb on + strand, within Synpcc7942_0473 at 459.492 kb on + strand at 459.500 kb on + strand, within Synpcc7942_0473 at 459.500 kb on + strand, within Synpcc7942_0473 at 459.505 kb on + strand, within Synpcc7942_0473 at 459.523 kb on - strand, within Synpcc7942_0473 at 459.524 kb on - strand, within Synpcc7942_0473 at 459.564 kb on + strand, within Synpcc7942_0473 at 459.564 kb on + strand, within Synpcc7942_0473 at 459.564 kb on + strand, within Synpcc7942_0473 at 459.564 kb on + strand, within Synpcc7942_0473 at 459.578 kb on - strand at 459.624 kb on - strand, within Synpcc7942_0473 at 459.624 kb on - strand, within Synpcc7942_0473 at 459.626 kb on - strand, within Synpcc7942_0473 at 459.626 kb on - strand, within Synpcc7942_0473 at 459.633 kb on + strand, within Synpcc7942_0473 at 459.665 kb on + strand, within Synpcc7942_0473 at 459.670 kb on + strand, within Synpcc7942_0473 at 459.670 kb on + strand, within Synpcc7942_0473 at 459.683 kb on - strand, within Synpcc7942_0473 at 459.689 kb on + strand, within Synpcc7942_0473 at 459.704 kb on - strand, within Synpcc7942_0473
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 456,959 + +0.1 456,959 + -0.1 456,966 - -0.4 456,967 - -0.0 456,967 - -2.9 456,967 - +0.2 456,969 + +0.7 456,974 + -2.1 456,977 - -3.3 456,982 - -1.9 456,982 - -3.5 456,992 + -1.6 457,044 - -3.5 457,060 + +2.0 457,136 + -0.8 457,137 + +0.2 457,159 + -0.5 457,162 - -3.9 457,164 - +3.7 457,167 - -0.3 457,186 - +0.8 457,205 - -0.5 457,260 - -2.0 457,299 - Synpcc7942_0470 0.16 -2.0 457,304 + Synpcc7942_0470 0.17 -1.5 457,304 + Synpcc7942_0470 0.17 -2.2 457,312 - Synpcc7942_0470 0.19 -3.0 457,312 - Synpcc7942_0470 0.19 -3.5 457,312 - Synpcc7942_0470 0.19 -2.5 457,312 - Synpcc7942_0470 0.19 -1.5 457,312 - Synpcc7942_0470 0.19 +1.7 457,346 + Synpcc7942_0470 0.25 +0.8 457,351 + Synpcc7942_0470 0.26 -1.8 457,385 + Synpcc7942_0470 0.33 -2.1 457,393 - Synpcc7942_0470 0.34 -0.6 457,412 + +0.5 457,441 + +3.0 457,441 + +0.6 457,477 - Synpcc7942_0470 0.50 -1.2 457,479 + Synpcc7942_0470 0.50 -1.7 457,482 - Synpcc7942_0470 0.51 +0.1 457,484 + Synpcc7942_0470 0.51 +2.8 457,490 + Synpcc7942_0470 0.52 +0.4 457,492 - Synpcc7942_0470 0.53 +0.2 457,492 - -1.2 457,508 - Synpcc7942_0470 0.56 -2.1 457,518 + Synpcc7942_0470 0.57 +1.9 457,525 - Synpcc7942_0470 0.59 -0.4 457,553 - Synpcc7942_0470 0.64 +1.3 457,572 + Synpcc7942_0470 0.68 +0.3 457,580 - Synpcc7942_0470 0.69 -2.2 457,582 + Synpcc7942_0470 0.69 +0.8 457,598 + Synpcc7942_0470 0.72 -2.2 457,605 - Synpcc7942_0470 0.74 +0.8 457,605 - Synpcc7942_0470 0.74 -2.4 457,608 + Synpcc7942_0470 0.74 +0.6 457,616 - Synpcc7942_0470 0.76 -2.1 457,647 - Synpcc7942_0470 0.82 -0.7 457,655 + Synpcc7942_0470 0.83 -1.3 457,692 - +0.1 457,692 - +1.5 457,757 - -1.4 457,780 - -2.9 457,785 + +2.0 457,785 + -2.4 457,793 - -0.1 457,803 - -0.8 457,843 + -2.0 457,870 + +2.9 457,880 - -0.9 457,884 + -2.2 457,887 - -1.7 457,920 - -2.1 457,922 - -2.4 457,922 - +0.5 457,934 + -2.0 457,989 - lacG Synpcc7942_0471 0.13 -0.5 457,989 - lacG Synpcc7942_0471 0.13 -2.3 458,005 - lacG Synpcc7942_0471 0.15 -3.0 458,086 + lacG Synpcc7942_0471 0.25 -2.8 458,094 - lacG Synpcc7942_0471 0.26 -1.6 458,124 - lacG Synpcc7942_0471 0.29 -4.5 458,137 + lacG Synpcc7942_0471 0.31 -1.1 458,145 + lacG Synpcc7942_0471 0.32 -4.2 458,161 + lacG Synpcc7942_0471 0.34 -4.9 458,169 - lacG Synpcc7942_0471 0.35 -0.2 458,169 - lacG Synpcc7942_0471 0.35 -1.7 458,254 + lacG Synpcc7942_0471 0.45 -3.5 458,262 + lacG Synpcc7942_0471 0.46 -2.5 458,264 + lacG Synpcc7942_0471 0.46 +0.4 458,264 + lacG Synpcc7942_0471 0.46 -0.9 458,274 - lacG Synpcc7942_0471 0.47 -1.1 458,279 + lacG Synpcc7942_0471 0.48 -3.2 458,279 + lacG Synpcc7942_0471 0.48 -1.2 458,289 + lacG Synpcc7942_0471 0.49 -2.4 458,294 + lacG Synpcc7942_0471 0.50 -4.8 458,294 + lacG Synpcc7942_0471 0.50 -1.5 458,297 - lacG Synpcc7942_0471 0.50 -2.3 458,302 - lacG Synpcc7942_0471 0.51 -1.5 458,424 - lacG Synpcc7942_0471 0.65 -3.6 458,483 - lacG Synpcc7942_0471 0.72 -0.7 458,490 + lacG Synpcc7942_0471 0.73 -1.6 458,545 + lacG Synpcc7942_0471 0.80 -0.7 458,550 + lacG Synpcc7942_0471 0.80 -1.7 458,551 + lacG Synpcc7942_0471 0.81 -2.0 458,551 + lacG Synpcc7942_0471 0.81 -2.2 458,559 - lacG Synpcc7942_0471 0.81 +1.4 458,572 + lacG Synpcc7942_0471 0.83 -0.7 458,625 - lacG Synpcc7942_0471 0.89 +0.1 458,626 - lacG Synpcc7942_0471 0.90 -2.3 458,667 + -1.4 458,667 + -1.4 458,667 + -4.7 458,667 + -1.5 458,675 - +0.7 458,706 - -2.6 458,707 + -0.6 458,714 - +1.6 458,752 + +1.0 458,767 + +1.0 458,772 + -1.8 458,803 + +0.9 458,803 + +1.9 458,811 - +3.1 458,861 + Synpcc7942_0472 0.29 -2.2 458,871 + Synpcc7942_0472 0.34 +0.1 458,879 - Synpcc7942_0472 0.37 -2.1 458,881 + Synpcc7942_0472 0.38 -0.2 458,889 - Synpcc7942_0472 0.42 -0.1 458,889 - Synpcc7942_0472 0.42 +2.9 458,974 - Synpcc7942_0472 0.80 +0.9 459,033 + +2.7 459,086 + +1.0 459,094 - +0.5 459,094 - +0.8 459,126 + +1.9 459,126 + +0.3 459,139 + +1.4 459,162 + +0.7 459,171 + -1.8 459,203 - +1.0 459,217 + Synpcc7942_0473 0.11 -1.7 459,225 - Synpcc7942_0473 0.12 -2.0 459,245 + Synpcc7942_0473 0.13 -1.6 459,281 + Synpcc7942_0473 0.17 -0.3 459,294 + Synpcc7942_0473 0.18 +0.1 459,297 - Synpcc7942_0473 0.18 -0.4 459,302 - Synpcc7942_0473 0.19 +0.5 459,302 - Synpcc7942_0473 0.19 +1.8 459,302 - Synpcc7942_0473 0.19 +1.4 459,313 + Synpcc7942_0473 0.20 -3.0 459,313 + Synpcc7942_0473 0.20 +1.5 459,315 - Synpcc7942_0473 0.20 +0.6 459,328 + Synpcc7942_0473 0.21 -0.7 459,328 + Synpcc7942_0473 0.21 -0.1 459,330 + Synpcc7942_0473 0.21 +1.7 459,336 - Synpcc7942_0473 0.22 -0.9 459,345 - Synpcc7942_0473 0.22 +0.3 459,346 - Synpcc7942_0473 0.23 -1.5 459,346 - Synpcc7942_0473 0.23 +0.1 459,375 - Synpcc7942_0473 0.25 -1.7 459,401 + Synpcc7942_0473 0.28 +0.8 459,445 + Synpcc7942_0473 0.32 -0.9 459,448 + Synpcc7942_0473 0.32 +3.6 459,455 + Synpcc7942_0473 0.32 -1.0 459,470 - Synpcc7942_0473 0.34 +0.3 459,491 + Synpcc7942_0473 0.36 +1.4 459,492 + +2.1 459,500 + Synpcc7942_0473 0.36 +1.4 459,500 + Synpcc7942_0473 0.36 +0.0 459,505 + Synpcc7942_0473 0.37 +0.5 459,523 - Synpcc7942_0473 0.39 +1.8 459,524 - Synpcc7942_0473 0.39 +1.4 459,564 + Synpcc7942_0473 0.42 +0.9 459,564 + Synpcc7942_0473 0.42 +0.0 459,564 + Synpcc7942_0473 0.42 +0.3 459,564 + Synpcc7942_0473 0.42 +3.1 459,578 - +1.1 459,624 - Synpcc7942_0473 0.48 -0.2 459,624 - Synpcc7942_0473 0.48 +0.9 459,626 - Synpcc7942_0473 0.48 -1.8 459,626 - Synpcc7942_0473 0.48 +0.7 459,633 + Synpcc7942_0473 0.49 -1.2 459,665 + Synpcc7942_0473 0.51 -1.7 459,670 + Synpcc7942_0473 0.52 -1.0 459,670 + Synpcc7942_0473 0.52 +0.9 459,683 - Synpcc7942_0473 0.53 -0.2 459,689 + Synpcc7942_0473 0.54 -0.8 459,704 - Synpcc7942_0473 0.55 -0.2
Or see this region's nucleotide sequence