Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0433 and tRNA-Arg are separated by 70 nucleotides tRNA-Arg and Synpcc7942_0434 are separated by 72 nucleotides Synpcc7942_0434 and Synpcc7942_0435 are separated by 10 nucleotides Synpcc7942_0435 and Synpcc7942_0436 are separated by 14 nucleotides
Synpcc7942_0433: Synpcc7942_0433 - hypothetical protein, at 423,632 to 424,840
_0433
Synpcc7942_R0031: tRNA-Arg - tRNA-Arg, at 424,911 to 424,984
tRNA-Arg
Synpcc7942_0434: Synpcc7942_0434 - hypothetical protein, at 425,057 to 426,064
_0434
Synpcc7942_0435: Synpcc7942_0435 - hypothetical protein, at 426,075 to 426,830
_0435
Synpcc7942_0436: Synpcc7942_0436 - hypothetical protein, at 426,845 to 427,150
_0436
Position (kb) 425
426
427 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 424.061 kb on + strand, within Synpcc7942_0433 at 424.068 kb on - strand, within Synpcc7942_0433 at 424.069 kb on - strand, within Synpcc7942_0433 at 424.084 kb on + strand, within Synpcc7942_0433 at 424.127 kb on + strand, within Synpcc7942_0433 at 424.135 kb on - strand, within Synpcc7942_0433 at 424.137 kb on + strand, within Synpcc7942_0433 at 424.198 kb on + strand, within Synpcc7942_0433 at 424.198 kb on + strand, within Synpcc7942_0433 at 424.206 kb on - strand, within Synpcc7942_0433 at 424.214 kb on - strand, within Synpcc7942_0433 at 424.220 kb on + strand, within Synpcc7942_0433 at 424.228 kb on - strand, within Synpcc7942_0433 at 424.228 kb on - strand, within Synpcc7942_0433 at 424.296 kb on - strand, within Synpcc7942_0433 at 424.366 kb on + strand, within Synpcc7942_0433 at 424.376 kb on - strand, within Synpcc7942_0433 at 424.384 kb on + strand, within Synpcc7942_0433 at 424.387 kb on - strand, within Synpcc7942_0433 at 424.453 kb on - strand, within Synpcc7942_0433 at 424.511 kb on + strand, within Synpcc7942_0433 at 424.567 kb on + strand, within Synpcc7942_0433 at 424.575 kb on - strand, within Synpcc7942_0433 at 424.595 kb on + strand, within Synpcc7942_0433 at 424.625 kb on - strand, within Synpcc7942_0433 at 424.644 kb on + strand, within Synpcc7942_0433 at 424.657 kb on - strand, within Synpcc7942_0433 at 424.783 kb on + strand at 424.786 kb on - strand at 424.806 kb on - strand at 424.835 kb on + strand at 424.847 kb on - strand at 424.863 kb on + strand at 424.871 kb on - strand at 424.937 kb on + strand, within tRNA-Arg at 424.937 kb on + strand, within tRNA-Arg at 424.937 kb on + strand, within tRNA-Arg at 424.937 kb on + strand, within tRNA-Arg at 424.945 kb on - strand, within tRNA-Arg at 424.962 kb on - strand, within tRNA-Arg at 425.002 kb on - strand at 425.002 kb on - strand at 425.012 kb on - strand at 425.049 kb on + strand at 425.071 kb on - strand at 425.076 kb on + strand at 425.076 kb on + strand at 425.076 kb on + strand at 425.076 kb on + strand at 425.084 kb on - strand at 425.086 kb on + strand at 425.086 kb on + strand at 425.094 kb on - strand at 425.094 kb on - strand at 425.094 kb on - strand at 425.226 kb on + strand, within Synpcc7942_0434 at 425.263 kb on - strand, within Synpcc7942_0434 at 425.334 kb on + strand, within Synpcc7942_0434 at 425.339 kb on - strand, within Synpcc7942_0434 at 425.440 kb on + strand, within Synpcc7942_0434 at 425.646 kb on + strand, within Synpcc7942_0434 at 425.661 kb on - strand, within Synpcc7942_0434 at 425.675 kb on + strand, within Synpcc7942_0434 at 425.680 kb on + strand, within Synpcc7942_0434 at 425.680 kb on + strand, within Synpcc7942_0434 at 425.683 kb on - strand, within Synpcc7942_0434 at 425.683 kb on - strand, within Synpcc7942_0434 at 425.688 kb on - strand, within Synpcc7942_0434 at 425.688 kb on - strand, within Synpcc7942_0434 at 425.696 kb on + strand, within Synpcc7942_0434 at 425.733 kb on + strand, within Synpcc7942_0434 at 425.749 kb on + strand, within Synpcc7942_0434 at 425.757 kb on + strand, within Synpcc7942_0434 at 425.757 kb on + strand, within Synpcc7942_0434 at 425.757 kb on - strand, within Synpcc7942_0434 at 425.758 kb on - strand, within Synpcc7942_0434 at 425.758 kb on - strand, within Synpcc7942_0434 at 425.765 kb on - strand, within Synpcc7942_0434 at 425.772 kb on + strand, within Synpcc7942_0434 at 425.772 kb on + strand, within Synpcc7942_0434 at 425.772 kb on + strand, within Synpcc7942_0434 at 425.772 kb on + strand, within Synpcc7942_0434 at 425.780 kb on - strand, within Synpcc7942_0434 at 425.780 kb on - strand, within Synpcc7942_0434 at 425.780 kb on - strand, within Synpcc7942_0434 at 425.785 kb on - strand, within Synpcc7942_0434 at 425.795 kb on + strand, within Synpcc7942_0434 at 425.798 kb on - strand, within Synpcc7942_0434 at 425.798 kb on - strand, within Synpcc7942_0434 at 425.814 kb on + strand, within Synpcc7942_0434 at 425.840 kb on + strand, within Synpcc7942_0434 at 425.843 kb on - strand, within Synpcc7942_0434 at 425.917 kb on - strand, within Synpcc7942_0434 at 425.960 kb on - strand, within Synpcc7942_0434 at 425.988 kb on - strand at 426.039 kb on - strand at 426.052 kb on - strand at 426.076 kb on - strand at 426.078 kb on + strand at 426.080 kb on + strand at 426.097 kb on - strand at 426.124 kb on + strand at 426.132 kb on - strand at 426.146 kb on - strand at 426.161 kb on + strand, within Synpcc7942_0435 at 426.205 kb on + strand, within Synpcc7942_0435 at 426.209 kb on + strand, within Synpcc7942_0435 at 426.213 kb on - strand, within Synpcc7942_0435 at 426.213 kb on - strand, within Synpcc7942_0435 at 426.265 kb on + strand, within Synpcc7942_0435 at 426.326 kb on + strand, within Synpcc7942_0435 at 426.391 kb on + strand, within Synpcc7942_0435 at 426.404 kb on - strand, within Synpcc7942_0435 at 426.498 kb on - strand, within Synpcc7942_0435 at 426.503 kb on + strand, within Synpcc7942_0435 at 426.511 kb on + strand, within Synpcc7942_0435 at 426.518 kb on + strand, within Synpcc7942_0435 at 426.518 kb on + strand, within Synpcc7942_0435 at 426.519 kb on - strand, within Synpcc7942_0435 at 426.519 kb on - strand, within Synpcc7942_0435 at 426.519 kb on - strand, within Synpcc7942_0435 at 426.519 kb on - strand, within Synpcc7942_0435 at 426.523 kb on + strand, within Synpcc7942_0435 at 426.531 kb on - strand, within Synpcc7942_0435 at 426.544 kb on - strand, within Synpcc7942_0435 at 426.549 kb on - strand, within Synpcc7942_0435 at 426.568 kb on + strand, within Synpcc7942_0435 at 426.583 kb on + strand, within Synpcc7942_0435 at 426.591 kb on + strand, within Synpcc7942_0435 at 426.619 kb on + strand, within Synpcc7942_0435 at 426.619 kb on + strand, within Synpcc7942_0435 at 426.629 kb on + strand, within Synpcc7942_0435 at 426.642 kb on - strand, within Synpcc7942_0435 at 426.655 kb on - strand, within Synpcc7942_0435 at 426.656 kb on - strand, within Synpcc7942_0435 at 426.695 kb on + strand, within Synpcc7942_0435 at 426.703 kb on - strand, within Synpcc7942_0435 at 426.703 kb on - strand, within Synpcc7942_0435 at 426.708 kb on + strand, within Synpcc7942_0435 at 426.708 kb on + strand, within Synpcc7942_0435 at 426.716 kb on - strand, within Synpcc7942_0435 at 426.730 kb on + strand, within Synpcc7942_0435 at 426.740 kb on + strand, within Synpcc7942_0435 at 426.809 kb on + strand at 426.824 kb on - strand at 426.839 kb on + strand at 426.857 kb on + strand at 426.903 kb on - strand, within Synpcc7942_0436 at 427.011 kb on - strand, within Synpcc7942_0436 at 427.025 kb on - strand, within Synpcc7942_0436 at 427.027 kb on + strand, within Synpcc7942_0436 at 427.027 kb on + strand, within Synpcc7942_0436 at 427.027 kb on + strand, within Synpcc7942_0436
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 424,061 + Synpcc7942_0433 0.35 -0.9 424,068 - Synpcc7942_0433 0.36 +0.4 424,069 - Synpcc7942_0433 0.36 -2.4 424,084 + Synpcc7942_0433 0.37 +3.3 424,127 + Synpcc7942_0433 0.41 -0.5 424,135 - Synpcc7942_0433 0.42 +3.7 424,137 + Synpcc7942_0433 0.42 +0.3 424,198 + Synpcc7942_0433 0.47 -1.8 424,198 + Synpcc7942_0433 0.47 -1.1 424,206 - Synpcc7942_0433 0.47 -0.5 424,214 - Synpcc7942_0433 0.48 +4.8 424,220 + Synpcc7942_0433 0.49 -0.7 424,228 - Synpcc7942_0433 0.49 -0.3 424,228 - Synpcc7942_0433 0.49 -2.2 424,296 - Synpcc7942_0433 0.55 +1.2 424,366 + Synpcc7942_0433 0.61 -0.8 424,376 - Synpcc7942_0433 0.62 +2.7 424,384 + Synpcc7942_0433 0.62 +1.1 424,387 - Synpcc7942_0433 0.62 +3.1 424,453 - Synpcc7942_0433 0.68 -0.0 424,511 + Synpcc7942_0433 0.73 +0.8 424,567 + Synpcc7942_0433 0.77 +1.0 424,575 - Synpcc7942_0433 0.78 +1.0 424,595 + Synpcc7942_0433 0.80 -1.7 424,625 - Synpcc7942_0433 0.82 +0.3 424,644 + Synpcc7942_0433 0.84 +1.2 424,657 - Synpcc7942_0433 0.85 -1.7 424,783 + -1.3 424,786 - -2.7 424,806 - -0.7 424,835 + -3.0 424,847 - -2.4 424,863 + -1.7 424,871 - -1.3 424,937 + tRNA-Arg Synpcc7942_R0031 0.35 -0.3 424,937 + tRNA-Arg Synpcc7942_R0031 0.35 -2.3 424,937 + tRNA-Arg Synpcc7942_R0031 0.35 +0.1 424,937 + tRNA-Arg Synpcc7942_R0031 0.35 -1.7 424,945 - tRNA-Arg Synpcc7942_R0031 0.46 -1.3 424,962 - tRNA-Arg Synpcc7942_R0031 0.69 -1.8 425,002 - -1.6 425,002 - -0.6 425,012 - -1.0 425,049 + -0.6 425,071 - -2.3 425,076 + -1.4 425,076 + -1.3 425,076 + -2.2 425,076 + -2.3 425,084 - -2.7 425,086 + -2.1 425,086 + -3.1 425,094 - -6.2 425,094 - -2.7 425,094 - -3.9 425,226 + Synpcc7942_0434 0.17 -0.1 425,263 - Synpcc7942_0434 0.20 -0.3 425,334 + Synpcc7942_0434 0.27 -2.6 425,339 - Synpcc7942_0434 0.28 -2.3 425,440 + Synpcc7942_0434 0.38 -2.1 425,646 + Synpcc7942_0434 0.58 -1.3 425,661 - Synpcc7942_0434 0.60 -4.2 425,675 + Synpcc7942_0434 0.61 -2.1 425,680 + Synpcc7942_0434 0.62 -2.6 425,680 + Synpcc7942_0434 0.62 -1.7 425,683 - Synpcc7942_0434 0.62 -1.4 425,683 - Synpcc7942_0434 0.62 -2.9 425,688 - Synpcc7942_0434 0.63 -3.7 425,688 - Synpcc7942_0434 0.63 -1.9 425,696 + Synpcc7942_0434 0.63 -1.6 425,733 + Synpcc7942_0434 0.67 -4.5 425,749 + Synpcc7942_0434 0.69 -2.4 425,757 + Synpcc7942_0434 0.69 -2.6 425,757 + Synpcc7942_0434 0.69 -4.0 425,757 - Synpcc7942_0434 0.69 +0.4 425,758 - Synpcc7942_0434 0.70 -1.8 425,758 - Synpcc7942_0434 0.70 -2.0 425,765 - Synpcc7942_0434 0.70 -1.4 425,772 + Synpcc7942_0434 0.71 +1.9 425,772 + Synpcc7942_0434 0.71 -3.1 425,772 + Synpcc7942_0434 0.71 -1.4 425,772 + Synpcc7942_0434 0.71 -3.8 425,780 - Synpcc7942_0434 0.72 -3.8 425,780 - Synpcc7942_0434 0.72 -2.3 425,780 - Synpcc7942_0434 0.72 -2.2 425,785 - Synpcc7942_0434 0.72 -1.1 425,795 + Synpcc7942_0434 0.73 -1.8 425,798 - Synpcc7942_0434 0.74 -3.7 425,798 - Synpcc7942_0434 0.74 -3.7 425,814 + Synpcc7942_0434 0.75 -1.4 425,840 + Synpcc7942_0434 0.78 -2.9 425,843 - Synpcc7942_0434 0.78 -3.6 425,917 - Synpcc7942_0434 0.85 -4.1 425,960 - Synpcc7942_0434 0.90 -3.9 425,988 - -3.9 426,039 - -1.8 426,052 - -2.0 426,076 - -1.7 426,078 + -2.6 426,080 + -0.5 426,097 - +1.6 426,124 + -3.3 426,132 - -3.7 426,146 - -3.0 426,161 + Synpcc7942_0435 0.11 -3.4 426,205 + Synpcc7942_0435 0.17 -3.8 426,209 + Synpcc7942_0435 0.18 -4.5 426,213 - Synpcc7942_0435 0.18 -1.9 426,213 - Synpcc7942_0435 0.18 -2.4 426,265 + Synpcc7942_0435 0.25 -3.7 426,326 + Synpcc7942_0435 0.33 -2.8 426,391 + Synpcc7942_0435 0.42 -1.7 426,404 - Synpcc7942_0435 0.44 -2.2 426,498 - Synpcc7942_0435 0.56 -5.1 426,503 + Synpcc7942_0435 0.57 -0.4 426,511 + Synpcc7942_0435 0.58 -0.7 426,518 + Synpcc7942_0435 0.59 -0.3 426,518 + Synpcc7942_0435 0.59 -2.8 426,519 - Synpcc7942_0435 0.59 -4.5 426,519 - Synpcc7942_0435 0.59 -4.2 426,519 - Synpcc7942_0435 0.59 -3.8 426,519 - Synpcc7942_0435 0.59 -2.1 426,523 + Synpcc7942_0435 0.59 -2.5 426,531 - Synpcc7942_0435 0.60 -2.2 426,544 - Synpcc7942_0435 0.62 -4.8 426,549 - Synpcc7942_0435 0.63 -3.1 426,568 + Synpcc7942_0435 0.65 -0.9 426,583 + Synpcc7942_0435 0.67 -4.1 426,591 + Synpcc7942_0435 0.68 -3.3 426,619 + Synpcc7942_0435 0.72 -2.8 426,619 + Synpcc7942_0435 0.72 -2.9 426,629 + Synpcc7942_0435 0.73 -3.7 426,642 - Synpcc7942_0435 0.75 -1.8 426,655 - Synpcc7942_0435 0.77 -2.7 426,656 - Synpcc7942_0435 0.77 -2.3 426,695 + Synpcc7942_0435 0.82 -0.9 426,703 - Synpcc7942_0435 0.83 -1.8 426,703 - Synpcc7942_0435 0.83 -2.9 426,708 + Synpcc7942_0435 0.84 -2.6 426,708 + Synpcc7942_0435 0.84 -2.9 426,716 - Synpcc7942_0435 0.85 -2.3 426,730 + Synpcc7942_0435 0.87 -4.2 426,740 + Synpcc7942_0435 0.88 -2.8 426,809 + -3.5 426,824 - -3.0 426,839 + -0.8 426,857 + -0.4 426,903 - Synpcc7942_0436 0.19 +0.5 427,011 - Synpcc7942_0436 0.54 +0.4 427,025 - Synpcc7942_0436 0.59 +2.4 427,027 + Synpcc7942_0436 0.59 +0.2 427,027 + Synpcc7942_0436 0.59 +0.7 427,027 + Synpcc7942_0436 0.59 -0.7
Or see this region's nucleotide sequence