Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt psbAI and Synpcc7942_0425 are separated by 108 nucleotides Synpcc7942_0425 and scpB are separated by 93 nucleotides scpB and Synpcc7942_0427 are separated by 16 nucleotides
Synpcc7942_0424: psbAI - photosystem q(b) protein, at 413,873 to 414,955
psbAI
Synpcc7942_0425: Synpcc7942_0425 - hypothetical protein, at 415,064 to 415,441
_0425
Synpcc7942_0426: scpB - condensin subunit ScpB, at 415,535 to 416,086
scpB
Synpcc7942_0427: Synpcc7942_0427 - ATPase, at 416,103 to 416,951
_0427
Position (kb) 415
416 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 414.065 kb on - strand, within psbAI at 414.068 kb on + strand, within psbAI at 414.089 kb on - strand, within psbAI at 414.089 kb on - strand, within psbAI at 414.190 kb on + strand, within psbAI at 414.283 kb on + strand, within psbAI at 414.298 kb on + strand, within psbAI at 414.325 kb on - strand, within psbAI at 414.699 kb on + strand, within psbAI at 414.710 kb on + strand, within psbAI at 414.760 kb on - strand, within psbAI at 414.838 kb on - strand, within psbAI at 415.061 kb on + strand at 415.061 kb on + strand at 415.069 kb on - strand at 415.216 kb on - strand, within Synpcc7942_0425 at 415.391 kb on + strand, within Synpcc7942_0425 at 415.393 kb on + strand, within Synpcc7942_0425 at 415.393 kb on + strand, within Synpcc7942_0425 at 415.401 kb on - strand, within Synpcc7942_0425 at 415.436 kb on - strand at 415.554 kb on + strand at 415.605 kb on + strand, within scpB at 415.607 kb on + strand, within scpB at 415.630 kb on - strand, within scpB at 415.648 kb on + strand, within scpB at 415.738 kb on - strand, within scpB at 415.738 kb on - strand, within scpB at 415.750 kb on + strand, within scpB at 415.758 kb on - strand, within scpB at 415.758 kb on - strand, within scpB at 415.758 kb on - strand, within scpB at 415.768 kb on - strand, within scpB at 415.817 kb on + strand, within scpB at 415.845 kb on + strand, within scpB at 415.879 kb on - strand, within scpB at 415.879 kb on - strand, within scpB at 415.912 kb on - strand, within scpB at 415.922 kb on + strand, within scpB at 415.975 kb on - strand, within scpB at 415.995 kb on + strand, within scpB at 416.003 kb on - strand, within scpB at 416.023 kb on + strand, within scpB at 416.023 kb on + strand, within scpB at 416.023 kb on + strand, within scpB at 416.023 kb on + strand, within scpB at 416.023 kb on + strand, within scpB at 416.023 kb on + strand, within scpB at 416.031 kb on - strand at 416.031 kb on - strand at 416.031 kb on - strand at 416.031 kb on - strand at 416.031 kb on - strand at 416.031 kb on - strand at 416.031 kb on - strand at 416.031 kb on - strand at 416.045 kb on - strand at 416.045 kb on - strand at 416.051 kb on - strand at 416.082 kb on - strand at 416.102 kb on - strand at 416.142 kb on - strand at 416.171 kb on - strand at 416.171 kb on - strand at 416.176 kb on - strand at 416.176 kb on - strand at 416.199 kb on + strand, within Synpcc7942_0427 at 416.201 kb on + strand, within Synpcc7942_0427 at 416.235 kb on + strand, within Synpcc7942_0427 at 416.237 kb on - strand, within Synpcc7942_0427 at 416.261 kb on + strand, within Synpcc7942_0427 at 416.262 kb on + strand, within Synpcc7942_0427 at 416.270 kb on - strand, within Synpcc7942_0427 at 416.326 kb on - strand, within Synpcc7942_0427 at 416.374 kb on - strand, within Synpcc7942_0427 at 416.376 kb on - strand, within Synpcc7942_0427 at 416.385 kb on + strand, within Synpcc7942_0427
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 414,065 - psbAI Synpcc7942_0424 0.18 +1.1 414,068 + psbAI Synpcc7942_0424 0.18 +3.4 414,089 - psbAI Synpcc7942_0424 0.20 +2.4 414,089 - psbAI Synpcc7942_0424 0.20 -1.4 414,190 + psbAI Synpcc7942_0424 0.29 +5.6 414,283 + psbAI Synpcc7942_0424 0.38 +2.1 414,298 + psbAI Synpcc7942_0424 0.39 +0.1 414,325 - psbAI Synpcc7942_0424 0.42 +2.9 414,699 + psbAI Synpcc7942_0424 0.76 +2.9 414,710 + psbAI Synpcc7942_0424 0.77 +3.5 414,760 - psbAI Synpcc7942_0424 0.82 +0.9 414,838 - psbAI Synpcc7942_0424 0.89 +3.8 415,061 + -1.0 415,061 + -1.5 415,069 - -3.2 415,216 - Synpcc7942_0425 0.40 -2.3 415,391 + Synpcc7942_0425 0.87 -1.8 415,393 + Synpcc7942_0425 0.87 -1.5 415,393 + Synpcc7942_0425 0.87 -2.3 415,401 - Synpcc7942_0425 0.89 -0.6 415,436 - -0.8 415,554 + -0.3 415,605 + scpB Synpcc7942_0426 0.13 -1.4 415,607 + scpB Synpcc7942_0426 0.13 -2.5 415,630 - scpB Synpcc7942_0426 0.17 -0.0 415,648 + scpB Synpcc7942_0426 0.20 -3.5 415,738 - scpB Synpcc7942_0426 0.37 -0.1 415,738 - scpB Synpcc7942_0426 0.37 +0.2 415,750 + scpB Synpcc7942_0426 0.39 +1.4 415,758 - scpB Synpcc7942_0426 0.40 -2.9 415,758 - scpB Synpcc7942_0426 0.40 +0.1 415,758 - scpB Synpcc7942_0426 0.40 +3.4 415,768 - scpB Synpcc7942_0426 0.42 +1.1 415,817 + scpB Synpcc7942_0426 0.51 -0.9 415,845 + scpB Synpcc7942_0426 0.56 -1.5 415,879 - scpB Synpcc7942_0426 0.62 -1.4 415,879 - scpB Synpcc7942_0426 0.62 +0.8 415,912 - scpB Synpcc7942_0426 0.68 -2.1 415,922 + scpB Synpcc7942_0426 0.70 -2.2 415,975 - scpB Synpcc7942_0426 0.80 -1.9 415,995 + scpB Synpcc7942_0426 0.83 -3.8 416,003 - scpB Synpcc7942_0426 0.85 -1.0 416,023 + scpB Synpcc7942_0426 0.88 +1.3 416,023 + scpB Synpcc7942_0426 0.88 -1.4 416,023 + scpB Synpcc7942_0426 0.88 +1.1 416,023 + scpB Synpcc7942_0426 0.88 +1.4 416,023 + scpB Synpcc7942_0426 0.88 -0.1 416,023 + scpB Synpcc7942_0426 0.88 -0.4 416,031 - +1.0 416,031 - -0.6 416,031 - -2.1 416,031 - +3.9 416,031 - -2.4 416,031 - -1.9 416,031 - -1.5 416,031 - -0.8 416,045 - +1.4 416,045 - +1.5 416,051 - -0.8 416,082 - +2.4 416,102 - -4.4 416,142 - -0.4 416,171 - +1.5 416,171 - +1.1 416,176 - +2.3 416,176 - -2.0 416,199 + Synpcc7942_0427 0.11 -0.4 416,201 + Synpcc7942_0427 0.12 -0.3 416,235 + Synpcc7942_0427 0.16 -0.9 416,237 - Synpcc7942_0427 0.16 -0.9 416,261 + Synpcc7942_0427 0.19 +2.3 416,262 + Synpcc7942_0427 0.19 -1.3 416,270 - Synpcc7942_0427 0.20 -0.9 416,326 - Synpcc7942_0427 0.26 -1.5 416,374 - Synpcc7942_0427 0.32 +1.7 416,376 - Synpcc7942_0427 0.32 +0.5 416,385 + Synpcc7942_0427 0.33 -3.0
Or see this region's nucleotide sequence