Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0415 and Synpcc7942_0416 are separated by 243 nucleotides Synpcc7942_0416 and Synpcc7942_0417 are separated by 78 nucleotides Synpcc7942_0417 and Synpcc7942_0418 are separated by 134 nucleotides Synpcc7942_0418 and bioB are separated by 96 nucleotides
Synpcc7942_0415: Synpcc7942_0415 - hypothetical protein, at 405,168 to 406,145
_0415
Synpcc7942_0416: Synpcc7942_0416 - hypothetical protein, at 406,389 to 406,772
_0416
Synpcc7942_0417: Synpcc7942_0417 - ATPase, at 406,851 to 408,359
_0417
Synpcc7942_0418: Synpcc7942_0418 - hypothetical protein, at 408,494 to 408,844
_0418
Synpcc7942_0419: bioB - biotin synthase, at 408,941 to 409,903
bioB
Position (kb)
406
407
408
409 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 405.856 kb on + strand, within Synpcc7942_0415 at 405.856 kb on + strand, within Synpcc7942_0415 at 405.884 kb on + strand, within Synpcc7942_0415 at 405.884 kb on + strand, within Synpcc7942_0415 at 405.884 kb on + strand, within Synpcc7942_0415 at 405.904 kb on + strand, within Synpcc7942_0415 at 405.907 kb on - strand, within Synpcc7942_0415 at 405.918 kb on + strand, within Synpcc7942_0415 at 405.939 kb on + strand, within Synpcc7942_0415 at 405.955 kb on + strand, within Synpcc7942_0415 at 405.958 kb on + strand, within Synpcc7942_0415 at 405.958 kb on + strand at 405.958 kb on + strand, within Synpcc7942_0415 at 406.008 kb on - strand, within Synpcc7942_0415 at 406.051 kb on + strand at 406.056 kb on + strand at 406.056 kb on + strand at 406.062 kb on + strand at 406.090 kb on + strand at 406.107 kb on - strand at 406.107 kb on - strand at 406.117 kb on - strand at 406.131 kb on + strand at 406.139 kb on - strand at 406.153 kb on + strand at 406.155 kb on + strand at 406.155 kb on + strand at 406.161 kb on - strand at 406.163 kb on - strand at 406.163 kb on - strand at 406.165 kb on + strand at 406.172 kb on + strand at 406.173 kb on - strand at 406.173 kb on - strand at 406.180 kb on - strand at 406.222 kb on - strand at 406.245 kb on + strand at 406.263 kb on - strand at 406.484 kb on + strand, within Synpcc7942_0416 at 406.500 kb on + strand, within Synpcc7942_0416 at 406.533 kb on + strand at 406.547 kb on + strand at 406.555 kb on - strand, within Synpcc7942_0416 at 406.555 kb on - strand, within Synpcc7942_0416 at 406.562 kb on + strand, within Synpcc7942_0416 at 406.583 kb on + strand, within Synpcc7942_0416 at 406.659 kb on + strand, within Synpcc7942_0416 at 406.667 kb on - strand, within Synpcc7942_0416 at 406.690 kb on - strand, within Synpcc7942_0416 at 406.690 kb on - strand, within Synpcc7942_0416 at 406.716 kb on + strand, within Synpcc7942_0416 at 406.724 kb on - strand, within Synpcc7942_0416 at 406.773 kb on + strand at 406.798 kb on + strand at 406.808 kb on - strand at 406.868 kb on - strand at 406.934 kb on + strand at 406.987 kb on - strand at 407.036 kb on + strand, within Synpcc7942_0417 at 407.039 kb on - strand, within Synpcc7942_0417 at 407.041 kb on + strand, within Synpcc7942_0417 at 407.044 kb on - strand, within Synpcc7942_0417 at 407.044 kb on - strand, within Synpcc7942_0417 at 407.049 kb on - strand, within Synpcc7942_0417 at 407.088 kb on - strand, within Synpcc7942_0417 at 407.132 kb on + strand, within Synpcc7942_0417 at 407.174 kb on + strand, within Synpcc7942_0417 at 407.184 kb on + strand, within Synpcc7942_0417 at 407.197 kb on - strand at 407.204 kb on + strand, within Synpcc7942_0417 at 407.283 kb on + strand, within Synpcc7942_0417 at 407.350 kb on + strand, within Synpcc7942_0417 at 407.376 kb on + strand, within Synpcc7942_0417 at 407.449 kb on + strand, within Synpcc7942_0417 at 407.556 kb on + strand at 407.612 kb on + strand, within Synpcc7942_0417 at 407.624 kb on + strand, within Synpcc7942_0417 at 407.624 kb on + strand, within Synpcc7942_0417 at 407.624 kb on + strand, within Synpcc7942_0417 at 407.657 kb on - strand, within Synpcc7942_0417 at 407.732 kb on + strand at 407.733 kb on + strand, within Synpcc7942_0417 at 407.740 kb on - strand, within Synpcc7942_0417 at 407.740 kb on - strand, within Synpcc7942_0417 at 407.756 kb on - strand, within Synpcc7942_0417 at 407.756 kb on - strand at 407.777 kb on - strand, within Synpcc7942_0417 at 407.850 kb on + strand, within Synpcc7942_0417 at 408.004 kb on + strand, within Synpcc7942_0417 at 408.101 kb on + strand, within Synpcc7942_0417 at 408.108 kb on - strand, within Synpcc7942_0417 at 408.179 kb on + strand, within Synpcc7942_0417 at 408.190 kb on + strand, within Synpcc7942_0417 at 408.213 kb on - strand at 408.223 kb on - strand at 408.262 kb on - strand at 408.303 kb on - strand at 408.318 kb on + strand at 408.388 kb on - strand at 408.394 kb on + strand at 408.404 kb on + strand at 408.456 kb on - strand at 408.497 kb on + strand at 408.504 kb on + strand at 408.504 kb on + strand at 408.504 kb on + strand at 408.541 kb on + strand, within Synpcc7942_0418 at 408.567 kb on + strand, within Synpcc7942_0418 at 408.601 kb on - strand, within Synpcc7942_0418 at 408.656 kb on + strand, within Synpcc7942_0418 at 408.687 kb on + strand, within Synpcc7942_0418 at 408.706 kb on + strand at 408.714 kb on - strand, within Synpcc7942_0418 at 408.714 kb on - strand, within Synpcc7942_0418 at 408.714 kb on - strand, within Synpcc7942_0418 at 408.806 kb on + strand, within Synpcc7942_0418 at 408.806 kb on + strand, within Synpcc7942_0418 at 408.814 kb on - strand at 408.814 kb on - strand at 408.844 kb on + strand at 408.844 kb on + strand at 408.881 kb on - strand at 408.881 kb on - strand at 408.881 kb on - strand at 408.908 kb on - strand at 408.938 kb on + strand at 408.942 kb on - strand at 408.953 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 405,856 + Synpcc7942_0415 0.70 -0.5 405,856 + Synpcc7942_0415 0.70 -0.3 405,884 + Synpcc7942_0415 0.73 +3.7 405,884 + Synpcc7942_0415 0.73 -0.6 405,884 + Synpcc7942_0415 0.73 -0.5 405,904 + Synpcc7942_0415 0.75 +1.0 405,907 - Synpcc7942_0415 0.76 -1.3 405,918 + Synpcc7942_0415 0.77 -0.1 405,939 + Synpcc7942_0415 0.79 +2.2 405,955 + Synpcc7942_0415 0.80 +3.7 405,958 + Synpcc7942_0415 0.81 +1.5 405,958 + -0.8 405,958 + Synpcc7942_0415 0.81 -2.1 406,008 - Synpcc7942_0415 0.86 +0.1 406,051 + +0.5 406,056 + +3.8 406,056 + +2.0 406,062 + +0.8 406,090 + -1.4 406,107 - -0.5 406,107 - -1.5 406,117 - -2.5 406,131 + +1.1 406,139 - +1.9 406,153 + +0.3 406,155 + +4.5 406,155 + -1.3 406,161 - -2.3 406,163 - +0.7 406,163 - +2.3 406,165 + +1.5 406,172 + +2.7 406,173 - +1.8 406,173 - +1.7 406,180 - +0.7 406,222 - +0.3 406,245 + +0.1 406,263 - +0.1 406,484 + Synpcc7942_0416 0.25 -2.5 406,500 + Synpcc7942_0416 0.29 -1.8 406,533 + +0.5 406,547 + +1.1 406,555 - Synpcc7942_0416 0.43 -2.4 406,555 - Synpcc7942_0416 0.43 -3.3 406,562 + Synpcc7942_0416 0.45 -4.3 406,583 + Synpcc7942_0416 0.51 -2.8 406,659 + Synpcc7942_0416 0.70 -1.3 406,667 - Synpcc7942_0416 0.72 -3.2 406,690 - Synpcc7942_0416 0.78 -0.9 406,690 - Synpcc7942_0416 0.78 -1.8 406,716 + Synpcc7942_0416 0.85 -2.0 406,724 - Synpcc7942_0416 0.87 -0.1 406,773 + -3.2 406,798 + -1.1 406,808 - -3.3 406,868 - -1.7 406,934 + -2.9 406,987 - -2.9 407,036 + Synpcc7942_0417 0.12 -2.7 407,039 - Synpcc7942_0417 0.12 -5.2 407,041 + Synpcc7942_0417 0.13 -2.1 407,044 - Synpcc7942_0417 0.13 -1.4 407,044 - Synpcc7942_0417 0.13 -4.1 407,049 - Synpcc7942_0417 0.13 -0.5 407,088 - Synpcc7942_0417 0.16 -3.8 407,132 + Synpcc7942_0417 0.19 -4.7 407,174 + Synpcc7942_0417 0.21 -1.4 407,184 + Synpcc7942_0417 0.22 -2.4 407,197 - -0.3 407,204 + Synpcc7942_0417 0.23 -1.8 407,283 + Synpcc7942_0417 0.29 -1.9 407,350 + Synpcc7942_0417 0.33 -2.8 407,376 + Synpcc7942_0417 0.35 -3.0 407,449 + Synpcc7942_0417 0.40 -2.8 407,556 + -1.3 407,612 + Synpcc7942_0417 0.50 -1.0 407,624 + Synpcc7942_0417 0.51 -4.1 407,624 + Synpcc7942_0417 0.51 -2.3 407,624 + Synpcc7942_0417 0.51 -1.8 407,657 - Synpcc7942_0417 0.53 -2.9 407,732 + -0.3 407,733 + Synpcc7942_0417 0.58 -3.2 407,740 - Synpcc7942_0417 0.59 +1.5 407,740 - Synpcc7942_0417 0.59 -2.9 407,756 - Synpcc7942_0417 0.60 -5.2 407,756 - -0.3 407,777 - Synpcc7942_0417 0.61 -1.4 407,850 + Synpcc7942_0417 0.66 -1.4 408,004 + Synpcc7942_0417 0.76 -1.3 408,101 + Synpcc7942_0417 0.83 +0.7 408,108 - Synpcc7942_0417 0.83 -2.3 408,179 + Synpcc7942_0417 0.88 -1.5 408,190 + Synpcc7942_0417 0.89 -0.4 408,213 - -3.6 408,223 - +1.7 408,262 - +0.1 408,303 - -2.5 408,318 + -0.9 408,388 - -1.0 408,394 + -2.2 408,404 + -2.9 408,456 - -1.9 408,497 + -2.4 408,504 + -3.1 408,504 + -1.2 408,504 + -0.4 408,541 + Synpcc7942_0418 0.13 -1.3 408,567 + Synpcc7942_0418 0.21 -2.5 408,601 - Synpcc7942_0418 0.30 -4.1 408,656 + Synpcc7942_0418 0.46 -2.0 408,687 + Synpcc7942_0418 0.55 -1.5 408,706 + +0.3 408,714 - Synpcc7942_0418 0.63 -1.1 408,714 - Synpcc7942_0418 0.63 -2.2 408,714 - Synpcc7942_0418 0.63 -3.8 408,806 + Synpcc7942_0418 0.89 +0.7 408,806 + Synpcc7942_0418 0.89 -1.4 408,814 - -2.5 408,814 - -1.6 408,844 + +0.2 408,844 + +1.4 408,881 - -0.1 408,881 - -1.5 408,881 - +1.8 408,908 - +0.8 408,938 + -1.1 408,942 - +0.6 408,953 - -0.6
Or see this region's nucleotide sequence