Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lim17 and Synpcc7942_0398 are separated by 66 nucleotides Synpcc7942_0398 and Synpcc7942_0399 are separated by 344 nucleotides Synpcc7942_0399 and trpG are separated by 33 nucleotides trpG and dgkA are separated by 64 nucleotides
Synpcc7942_0397: lim17 - putative neutral invertase, at 391,532 to 392,923
lim17
Synpcc7942_0398: Synpcc7942_0398 - hypothetical protein, at 392,990 to 393,448
_0398
Synpcc7942_0399: Synpcc7942_0399 - hypothetical protein, at 393,793 to 394,545
_0399
Synpcc7942_0400: trpG - anthranilate synthase, component II, at 394,579 to 395,181
trpG
Synpcc7942_0401: dgkA - diacylglycerol kinase, at 395,246 to 395,731
dgkA
Position (kb) 393
394
395 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 392.797 kb on - strand at 392.833 kb on + strand at 392.833 kb on + strand at 392.862 kb on + strand at 392.880 kb on - strand at 392.881 kb on + strand at 392.881 kb on + strand at 392.881 kb on + strand at 392.881 kb on + strand at 392.881 kb on + strand at 392.881 kb on + strand at 392.881 kb on + strand at 392.883 kb on + strand at 392.889 kb on - strand at 392.889 kb on - strand at 392.934 kb on + strand at 392.961 kb on - strand at 392.961 kb on - strand at 392.963 kb on + strand at 392.971 kb on - strand at 392.971 kb on - strand at 392.981 kb on + strand at 392.999 kb on + strand at 393.060 kb on + strand, within Synpcc7942_0398 at 393.171 kb on + strand, within Synpcc7942_0398 at 393.194 kb on + strand, within Synpcc7942_0398 at 393.194 kb on + strand, within Synpcc7942_0398 at 393.194 kb on + strand, within Synpcc7942_0398 at 393.194 kb on + strand, within Synpcc7942_0398 at 393.194 kb on + strand, within Synpcc7942_0398 at 393.194 kb on + strand, within Synpcc7942_0398 at 393.194 kb on + strand, within Synpcc7942_0398 at 393.196 kb on + strand, within Synpcc7942_0398 at 393.202 kb on - strand, within Synpcc7942_0398 at 393.202 kb on - strand, within Synpcc7942_0398 at 393.202 kb on - strand, within Synpcc7942_0398 at 393.202 kb on - strand, within Synpcc7942_0398 at 393.202 kb on - strand, within Synpcc7942_0398 at 393.202 kb on - strand, within Synpcc7942_0398 at 393.226 kb on - strand, within Synpcc7942_0398 at 393.280 kb on - strand, within Synpcc7942_0398 at 393.308 kb on + strand at 393.308 kb on + strand, within Synpcc7942_0398 at 393.308 kb on + strand, within Synpcc7942_0398 at 393.313 kb on + strand, within Synpcc7942_0398 at 393.313 kb on + strand, within Synpcc7942_0398 at 393.313 kb on + strand, within Synpcc7942_0398 at 393.362 kb on + strand, within Synpcc7942_0398 at 393.362 kb on + strand, within Synpcc7942_0398 at 393.362 kb on + strand, within Synpcc7942_0398 at 393.370 kb on - strand, within Synpcc7942_0398 at 393.370 kb on - strand, within Synpcc7942_0398 at 393.370 kb on - strand, within Synpcc7942_0398 at 393.380 kb on + strand, within Synpcc7942_0398 at 393.386 kb on - strand, within Synpcc7942_0398 at 393.410 kb on + strand at 393.425 kb on - strand at 393.446 kb on - strand at 393.465 kb on + strand at 393.492 kb on - strand at 393.492 kb on - strand at 393.492 kb on - strand at 393.492 kb on - strand at 393.502 kb on - strand at 393.541 kb on - strand at 393.591 kb on + strand at 393.591 kb on + strand at 393.591 kb on + strand at 393.591 kb on + strand at 393.591 kb on + strand at 393.592 kb on + strand at 393.592 kb on + strand at 393.592 kb on + strand at 393.603 kb on + strand at 393.604 kb on + strand at 393.604 kb on + strand at 393.606 kb on - strand at 393.611 kb on - strand at 393.612 kb on - strand at 393.635 kb on - strand at 393.679 kb on - strand at 393.700 kb on + strand at 393.700 kb on + strand at 393.705 kb on - strand at 393.706 kb on - strand at 393.746 kb on + strand at 393.757 kb on + strand at 393.757 kb on + strand at 393.757 kb on + strand at 393.765 kb on - strand at 393.765 kb on - strand at 393.767 kb on + strand at 393.767 kb on + strand at 393.791 kb on + strand at 393.794 kb on - strand at 393.872 kb on + strand, within Synpcc7942_0399 at 393.882 kb on + strand, within Synpcc7942_0399 at 393.908 kb on - strand, within Synpcc7942_0399 at 393.915 kb on + strand, within Synpcc7942_0399 at 393.933 kb on + strand, within Synpcc7942_0399 at 393.957 kb on - strand, within Synpcc7942_0399 at 393.974 kb on + strand, within Synpcc7942_0399 at 394.003 kb on - strand, within Synpcc7942_0399 at 394.005 kb on + strand, within Synpcc7942_0399 at 394.050 kb on - strand, within Synpcc7942_0399 at 394.050 kb on - strand, within Synpcc7942_0399 at 394.050 kb on - strand, within Synpcc7942_0399 at 394.050 kb on - strand, within Synpcc7942_0399 at 394.061 kb on - strand, within Synpcc7942_0399 at 394.061 kb on - strand, within Synpcc7942_0399 at 394.071 kb on - strand, within Synpcc7942_0399 at 394.103 kb on + strand, within Synpcc7942_0399 at 394.205 kb on + strand, within Synpcc7942_0399 at 394.220 kb on + strand, within Synpcc7942_0399 at 394.279 kb on + strand, within Synpcc7942_0399 at 394.307 kb on - strand, within Synpcc7942_0399 at 394.322 kb on - strand, within Synpcc7942_0399 at 394.342 kb on + strand, within Synpcc7942_0399 at 394.367 kb on + strand, within Synpcc7942_0399 at 394.431 kb on - strand, within Synpcc7942_0399 at 394.456 kb on + strand, within Synpcc7942_0399 at 394.457 kb on + strand, within Synpcc7942_0399 at 394.459 kb on - strand, within Synpcc7942_0399 at 394.462 kb on + strand, within Synpcc7942_0399 at 394.477 kb on + strand at 394.570 kb on + strand at 395.240 kb on - strand at 395.263 kb on + strand at 395.276 kb on - strand at 395.293 kb on - strand at 395.293 kb on - strand at 395.298 kb on + strand, within dgkA at 395.324 kb on + strand, within dgkA at 395.332 kb on - strand, within dgkA at 395.332 kb on - strand, within dgkA at 395.332 kb on - strand, within dgkA at 395.332 kb on - strand, within dgkA at 395.416 kb on - strand at 395.416 kb on - strand, within dgkA at 395.416 kb on - strand, within dgkA at 395.418 kb on + strand at 395.418 kb on + strand, within dgkA at 395.418 kb on + strand, within dgkA at 395.423 kb on + strand, within dgkA at 395.426 kb on - strand, within dgkA at 395.452 kb on + strand, within dgkA at 395.459 kb on + strand, within dgkA at 395.459 kb on + strand, within dgkA at 395.459 kb on + strand, within dgkA at 395.466 kb on - strand, within dgkA at 395.467 kb on - strand, within dgkA at 395.467 kb on - strand, within dgkA at 395.467 kb on - strand, within dgkA at 395.467 kb on - strand, within dgkA at 395.476 kb on + strand, within dgkA at 395.481 kb on + strand, within dgkA at 395.488 kb on + strand, within dgkA at 395.491 kb on - strand, within dgkA at 395.496 kb on - strand, within dgkA at 395.496 kb on - strand, within dgkA at 395.496 kb on - strand, within dgkA at 395.527 kb on + strand, within dgkA at 395.535 kb on - strand, within dgkA
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 392,797 - +6.7 392,833 + +5.7 392,833 + +5.2 392,862 + +4.4 392,880 - +3.2 392,881 + -2.1 392,881 + -1.8 392,881 + +1.4 392,881 + +2.3 392,881 + +1.0 392,881 + -2.0 392,881 + -3.4 392,883 + +4.2 392,889 - -0.6 392,889 - +4.8 392,934 + -0.1 392,961 - +2.6 392,961 - -3.4 392,963 + +2.4 392,971 - -1.5 392,971 - +0.2 392,981 + -2.7 392,999 + -1.4 393,060 + Synpcc7942_0398 0.15 +3.0 393,171 + Synpcc7942_0398 0.39 +0.5 393,194 + Synpcc7942_0398 0.44 -0.9 393,194 + Synpcc7942_0398 0.44 -0.5 393,194 + Synpcc7942_0398 0.44 -1.4 393,194 + Synpcc7942_0398 0.44 +0.0 393,194 + Synpcc7942_0398 0.44 -1.8 393,194 + Synpcc7942_0398 0.44 +1.3 393,194 + Synpcc7942_0398 0.44 -2.5 393,196 + Synpcc7942_0398 0.45 -1.4 393,202 - Synpcc7942_0398 0.46 -1.5 393,202 - Synpcc7942_0398 0.46 -1.7 393,202 - Synpcc7942_0398 0.46 +1.3 393,202 - Synpcc7942_0398 0.46 +1.9 393,202 - Synpcc7942_0398 0.46 -2.8 393,202 - Synpcc7942_0398 0.46 +0.8 393,226 - Synpcc7942_0398 0.51 +0.5 393,280 - Synpcc7942_0398 0.63 -0.3 393,308 + +0.9 393,308 + Synpcc7942_0398 0.69 -2.2 393,308 + Synpcc7942_0398 0.69 -0.8 393,313 + Synpcc7942_0398 0.70 -0.5 393,313 + Synpcc7942_0398 0.70 -0.2 393,313 + Synpcc7942_0398 0.70 -1.1 393,362 + Synpcc7942_0398 0.81 -0.5 393,362 + Synpcc7942_0398 0.81 +0.9 393,362 + Synpcc7942_0398 0.81 -1.8 393,370 - Synpcc7942_0398 0.83 +6.7 393,370 - Synpcc7942_0398 0.83 +1.0 393,370 - Synpcc7942_0398 0.83 -0.5 393,380 + Synpcc7942_0398 0.85 -1.4 393,386 - Synpcc7942_0398 0.86 +0.2 393,410 + +0.0 393,425 - -1.3 393,446 - +3.0 393,465 + -2.7 393,492 - +2.4 393,492 - -2.0 393,492 - -0.2 393,492 - +0.6 393,502 - +3.2 393,541 - +1.5 393,591 + -1.0 393,591 + +0.5 393,591 + -1.7 393,591 + -3.5 393,591 + -1.4 393,592 + +2.3 393,592 + +1.3 393,592 + +1.5 393,603 + -2.0 393,604 + +0.5 393,604 + +0.6 393,606 - +1.5 393,611 - +0.5 393,612 - +0.6 393,635 - -0.8 393,679 - -1.5 393,700 + +0.9 393,700 + +0.6 393,705 - +1.5 393,706 - +1.4 393,746 + -1.1 393,757 + +2.4 393,757 + -3.6 393,757 + +0.2 393,765 - +0.0 393,765 - +2.2 393,767 + +0.2 393,767 + +2.3 393,791 + -1.8 393,794 - +0.3 393,872 + Synpcc7942_0399 0.10 -1.5 393,882 + Synpcc7942_0399 0.12 -2.8 393,908 - Synpcc7942_0399 0.15 -3.9 393,915 + Synpcc7942_0399 0.16 -3.1 393,933 + Synpcc7942_0399 0.19 -3.6 393,957 - Synpcc7942_0399 0.22 -1.7 393,974 + Synpcc7942_0399 0.24 +1.6 394,003 - Synpcc7942_0399 0.28 -1.5 394,005 + Synpcc7942_0399 0.28 -2.4 394,050 - Synpcc7942_0399 0.34 -0.5 394,050 - Synpcc7942_0399 0.34 -3.4 394,050 - Synpcc7942_0399 0.34 -3.4 394,050 - Synpcc7942_0399 0.34 -1.5 394,061 - Synpcc7942_0399 0.36 -4.4 394,061 - Synpcc7942_0399 0.36 -1.8 394,071 - Synpcc7942_0399 0.37 -2.5 394,103 + Synpcc7942_0399 0.41 -3.9 394,205 + Synpcc7942_0399 0.55 -4.8 394,220 + Synpcc7942_0399 0.57 -3.0 394,279 + Synpcc7942_0399 0.65 -3.7 394,307 - Synpcc7942_0399 0.68 -1.4 394,322 - Synpcc7942_0399 0.70 -2.8 394,342 + Synpcc7942_0399 0.73 -3.4 394,367 + Synpcc7942_0399 0.76 -1.5 394,431 - Synpcc7942_0399 0.85 -0.3 394,456 + Synpcc7942_0399 0.88 -3.1 394,457 + Synpcc7942_0399 0.88 -1.1 394,459 - Synpcc7942_0399 0.88 -3.5 394,462 + Synpcc7942_0399 0.89 -1.2 394,477 + -2.1 394,570 + -2.3 395,240 - +1.8 395,263 + -3.2 395,276 - -0.7 395,293 - -4.1 395,293 - -2.9 395,298 + dgkA Synpcc7942_0401 0.11 -5.6 395,324 + dgkA Synpcc7942_0401 0.16 -5.8 395,332 - dgkA Synpcc7942_0401 0.18 -5.0 395,332 - dgkA Synpcc7942_0401 0.18 -1.5 395,332 - dgkA Synpcc7942_0401 0.18 -1.3 395,332 - dgkA Synpcc7942_0401 0.18 -2.6 395,416 - -2.0 395,416 - dgkA Synpcc7942_0401 0.35 -4.2 395,416 - dgkA Synpcc7942_0401 0.35 -5.5 395,418 + -1.7 395,418 + dgkA Synpcc7942_0401 0.35 -2.7 395,418 + dgkA Synpcc7942_0401 0.35 -4.4 395,423 + dgkA Synpcc7942_0401 0.36 -3.8 395,426 - dgkA Synpcc7942_0401 0.37 -4.0 395,452 + dgkA Synpcc7942_0401 0.42 -4.1 395,459 + dgkA Synpcc7942_0401 0.44 -5.9 395,459 + dgkA Synpcc7942_0401 0.44 -1.8 395,459 + dgkA Synpcc7942_0401 0.44 -3.8 395,466 - dgkA Synpcc7942_0401 0.45 -4.5 395,467 - dgkA Synpcc7942_0401 0.45 -4.1 395,467 - dgkA Synpcc7942_0401 0.45 -2.4 395,467 - dgkA Synpcc7942_0401 0.45 -2.3 395,467 - dgkA Synpcc7942_0401 0.45 -5.1 395,476 + dgkA Synpcc7942_0401 0.47 -4.4 395,481 + dgkA Synpcc7942_0401 0.48 -2.7 395,488 + dgkA Synpcc7942_0401 0.50 -4.8 395,491 - dgkA Synpcc7942_0401 0.50 -3.0 395,496 - dgkA Synpcc7942_0401 0.51 -5.5 395,496 - dgkA Synpcc7942_0401 0.51 -3.7 395,496 - dgkA Synpcc7942_0401 0.51 -4.5 395,527 + dgkA Synpcc7942_0401 0.58 -4.3 395,535 - dgkA Synpcc7942_0401 0.59 -2.8
Or see this region's nucleotide sequence