Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0318 and Synpcc7942_0319 are separated by 64 nucleotides Synpcc7942_0319 and galE are separated by 29 nucleotides galE and glnB are separated by 138 nucleotides glnB and ycf44 are separated by 193 nucleotides
Synpcc7942_0318: Synpcc7942_0318 - hypothetical protein, at 315,642 to 316,097
_0318
Synpcc7942_0319: Synpcc7942_0319 - hemolysin A, at 316,162 to 316,980
_0319
Synpcc7942_0320: galE - UDP-galactose 4-epimerase, at 317,010 to 318,008
galE
Synpcc7942_0321: glnB - nitrogen regulatory protein P-II, at 318,147 to 318,485
glnB
Synpcc7942_0322: ycf44 - c-type cytochrome biogenesis protein, at 318,679 to 320,049
ycf44
Position (kb) 317
318
319 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 316.038 kb on - strand, within Synpcc7942_0318 at 316.053 kb on - strand at 316.055 kb on + strand at 316.080 kb on + strand at 316.080 kb on + strand at 316.080 kb on + strand at 316.080 kb on + strand at 316.080 kb on + strand at 316.088 kb on - strand at 316.088 kb on - strand at 316.088 kb on - strand at 316.093 kb on + strand at 316.103 kb on + strand at 316.103 kb on + strand at 316.106 kb on - strand at 316.111 kb on - strand at 316.111 kb on - strand at 316.111 kb on - strand at 316.125 kb on + strand at 316.141 kb on + strand at 316.149 kb on - strand at 316.159 kb on - strand at 316.165 kb on + strand at 316.171 kb on - strand at 316.173 kb on - strand at 316.184 kb on - strand at 316.196 kb on + strand at 316.203 kb on + strand at 316.204 kb on - strand at 316.231 kb on + strand at 316.233 kb on - strand at 316.254 kb on - strand, within Synpcc7942_0319 at 316.269 kb on - strand, within Synpcc7942_0319 at 316.297 kb on - strand, within Synpcc7942_0319 at 316.340 kb on - strand, within Synpcc7942_0319 at 316.362 kb on + strand, within Synpcc7942_0319 at 316.405 kb on - strand, within Synpcc7942_0319 at 316.417 kb on - strand, within Synpcc7942_0319 at 316.474 kb on + strand, within Synpcc7942_0319 at 316.474 kb on + strand, within Synpcc7942_0319 at 316.481 kb on + strand, within Synpcc7942_0319 at 316.481 kb on + strand, within Synpcc7942_0319 at 316.482 kb on - strand, within Synpcc7942_0319 at 316.515 kb on + strand, within Synpcc7942_0319 at 316.582 kb on - strand, within Synpcc7942_0319 at 316.589 kb on - strand, within Synpcc7942_0319 at 316.659 kb on - strand, within Synpcc7942_0319 at 316.669 kb on - strand, within Synpcc7942_0319 at 316.669 kb on - strand, within Synpcc7942_0319 at 316.704 kb on + strand, within Synpcc7942_0319 at 316.709 kb on + strand, within Synpcc7942_0319 at 316.716 kb on + strand, within Synpcc7942_0319 at 316.716 kb on + strand, within Synpcc7942_0319 at 316.721 kb on + strand, within Synpcc7942_0319 at 316.807 kb on + strand, within Synpcc7942_0319 at 316.807 kb on + strand, within Synpcc7942_0319 at 316.815 kb on - strand, within Synpcc7942_0319 at 316.829 kb on + strand, within Synpcc7942_0319 at 316.829 kb on + strand, within Synpcc7942_0319 at 316.834 kb on + strand, within Synpcc7942_0319 at 316.842 kb on - strand, within Synpcc7942_0319 at 316.871 kb on + strand, within Synpcc7942_0319 at 316.884 kb on + strand, within Synpcc7942_0319 at 316.941 kb on + strand at 316.941 kb on + strand at 316.949 kb on - strand at 316.949 kb on - strand at 316.949 kb on - strand at 316.961 kb on - strand at 316.963 kb on + strand at 316.988 kb on - strand at 316.993 kb on + strand at 316.993 kb on + strand at 318.028 kb on + strand at 318.028 kb on + strand at 318.036 kb on - strand at 318.054 kb on - strand at 318.119 kb on + strand at 318.127 kb on - strand at 318.144 kb on + strand at 318.152 kb on - strand at 318.152 kb on - strand at 318.152 kb on - strand at 318.152 kb on - strand at 318.503 kb on - strand at 318.508 kb on - strand at 318.516 kb on - strand at 318.554 kb on + strand at 318.559 kb on + strand at 318.589 kb on - strand at 318.628 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 316,038 - Synpcc7942_0318 0.87 -2.6 316,053 - -0.3 316,055 + -0.6 316,080 + -1.5 316,080 + -1.5 316,080 + +1.5 316,080 + -3.2 316,080 + -0.5 316,088 - -1.3 316,088 - +2.0 316,088 - -0.7 316,093 + +1.1 316,103 + -2.1 316,103 + +0.5 316,106 - -0.3 316,111 - +2.0 316,111 - -2.0 316,111 - +1.6 316,125 + -1.3 316,141 + -0.7 316,149 - -0.5 316,159 - +4.2 316,165 + +0.1 316,171 - +2.9 316,173 - +0.3 316,184 - -4.6 316,196 + -1.8 316,203 + -1.2 316,204 - -2.8 316,231 + -2.6 316,233 - -1.5 316,254 - Synpcc7942_0319 0.11 -0.7 316,269 - Synpcc7942_0319 0.13 -2.9 316,297 - Synpcc7942_0319 0.16 -1.9 316,340 - Synpcc7942_0319 0.22 -0.9 316,362 + Synpcc7942_0319 0.24 -2.2 316,405 - Synpcc7942_0319 0.30 -4.3 316,417 - Synpcc7942_0319 0.31 -3.8 316,474 + Synpcc7942_0319 0.38 -2.1 316,474 + Synpcc7942_0319 0.38 -2.2 316,481 + Synpcc7942_0319 0.39 -4.1 316,481 + Synpcc7942_0319 0.39 -4.1 316,482 - Synpcc7942_0319 0.39 -2.9 316,515 + Synpcc7942_0319 0.43 -1.8 316,582 - Synpcc7942_0319 0.51 -2.9 316,589 - Synpcc7942_0319 0.52 -3.9 316,659 - Synpcc7942_0319 0.61 -3.2 316,669 - Synpcc7942_0319 0.62 +0.3 316,669 - Synpcc7942_0319 0.62 -3.5 316,704 + Synpcc7942_0319 0.66 -2.8 316,709 + Synpcc7942_0319 0.67 -3.2 316,716 + Synpcc7942_0319 0.68 -3.6 316,716 + Synpcc7942_0319 0.68 -3.6 316,721 + Synpcc7942_0319 0.68 -3.1 316,807 + Synpcc7942_0319 0.79 -3.0 316,807 + Synpcc7942_0319 0.79 -1.4 316,815 - Synpcc7942_0319 0.80 -0.9 316,829 + Synpcc7942_0319 0.81 -3.3 316,829 + Synpcc7942_0319 0.81 -1.1 316,834 + Synpcc7942_0319 0.82 -2.8 316,842 - Synpcc7942_0319 0.83 -0.5 316,871 + Synpcc7942_0319 0.87 -2.7 316,884 + Synpcc7942_0319 0.88 -4.3 316,941 + -0.5 316,941 + -0.2 316,949 - -3.7 316,949 - -3.3 316,949 - +0.3 316,961 - +1.6 316,963 + +0.3 316,988 - +0.8 316,993 + +3.0 316,993 + -0.2 318,028 + +2.2 318,028 + +2.2 318,036 - +3.4 318,054 - +3.6 318,119 + -0.7 318,127 - +3.6 318,144 + +0.1 318,152 - +3.7 318,152 - +5.8 318,152 - +0.9 318,152 - -0.3 318,503 - -0.5 318,508 - +2.3 318,516 - +1.2 318,554 + +0.7 318,559 + +0.5 318,589 - +4.0 318,628 + +3.2
Or see this region's nucleotide sequence