Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purB and digD are separated by 57 nucleotides digD and Synpcc7942_0317 are separated by 273 nucleotides Synpcc7942_0317 and Synpcc7942_0318 are separated by 286 nucleotides Synpcc7942_0318 and Synpcc7942_0319 are separated by 64 nucleotides
Synpcc7942_0315: purB - adenylosuccinate lyase, at 313,079 to 314,374
purB
Synpcc7942_0316: digD - hypothetical protein, at 314,432 to 314,623
digD
Synpcc7942_0317: Synpcc7942_0317 - hypothetical protein, at 314,897 to 315,355
_0317
Synpcc7942_0318: Synpcc7942_0318 - hypothetical protein, at 315,642 to 316,097
_0318
Synpcc7942_0319: Synpcc7942_0319 - hemolysin A, at 316,162 to 316,980
_0319
Position (kb) 314
315
316 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7 at 314.438 kb on - strand at 314.478 kb on + strand, within digD at 314.484 kb on + strand, within digD at 314.486 kb on - strand, within digD at 314.486 kb on - strand, within digD at 314.486 kb on - strand, within digD at 314.491 kb on - strand, within digD at 314.497 kb on + strand, within digD at 314.505 kb on - strand, within digD at 314.536 kb on - strand, within digD at 314.538 kb on + strand, within digD at 314.550 kb on + strand, within digD at 314.558 kb on - strand, within digD at 314.562 kb on + strand, within digD at 314.562 kb on + strand, within digD at 314.562 kb on + strand, within digD at 314.570 kb on - strand, within digD at 314.570 kb on - strand, within digD at 314.580 kb on - strand at 314.625 kb on + strand at 314.647 kb on + strand at 314.647 kb on + strand at 314.647 kb on + strand at 314.647 kb on + strand at 314.655 kb on - strand at 314.660 kb on - strand at 314.666 kb on - strand at 314.666 kb on - strand at 314.758 kb on - strand at 314.770 kb on - strand at 314.795 kb on + strand at 314.795 kb on + strand at 314.803 kb on - strand at 314.824 kb on - strand at 314.848 kb on + strand at 314.872 kb on - strand at 314.891 kb on + strand at 314.892 kb on + strand at 314.896 kb on + strand at 314.908 kb on + strand at 314.928 kb on + strand at 314.941 kb on + strand at 314.946 kb on + strand, within Synpcc7942_0317 at 314.946 kb on + strand, within Synpcc7942_0317 at 314.949 kb on - strand, within Synpcc7942_0317 at 314.949 kb on - strand, within Synpcc7942_0317 at 314.954 kb on - strand, within Synpcc7942_0317 at 314.971 kb on + strand, within Synpcc7942_0317 at 314.979 kb on - strand at 314.995 kb on + strand, within Synpcc7942_0317 at 315.042 kb on + strand, within Synpcc7942_0317 at 315.042 kb on + strand, within Synpcc7942_0317 at 315.086 kb on - strand, within Synpcc7942_0317 at 315.095 kb on + strand, within Synpcc7942_0317 at 315.096 kb on - strand, within Synpcc7942_0317 at 315.103 kb on - strand, within Synpcc7942_0317 at 315.126 kb on + strand, within Synpcc7942_0317 at 315.138 kb on + strand, within Synpcc7942_0317 at 315.142 kb on + strand, within Synpcc7942_0317 at 315.142 kb on - strand, within Synpcc7942_0317 at 315.146 kb on - strand, within Synpcc7942_0317 at 315.150 kb on - strand, within Synpcc7942_0317 at 315.160 kb on - strand, within Synpcc7942_0317 at 315.189 kb on + strand, within Synpcc7942_0317 at 315.213 kb on + strand, within Synpcc7942_0317 at 315.221 kb on - strand, within Synpcc7942_0317 at 315.234 kb on + strand, within Synpcc7942_0317 at 315.242 kb on - strand, within Synpcc7942_0317 at 315.242 kb on - strand, within Synpcc7942_0317 at 315.254 kb on - strand, within Synpcc7942_0317 at 315.297 kb on - strand, within Synpcc7942_0317 at 315.302 kb on + strand, within Synpcc7942_0317 at 315.302 kb on + strand, within Synpcc7942_0317 at 315.318 kb on + strand at 315.322 kb on + strand at 315.323 kb on + strand at 315.324 kb on + strand at 315.332 kb on - strand at 315.332 kb on - strand at 315.354 kb on - strand at 315.413 kb on - strand at 315.418 kb on - strand at 315.423 kb on - strand at 315.433 kb on - strand at 315.442 kb on + strand at 315.458 kb on - strand at 315.485 kb on + strand at 315.493 kb on + strand at 315.493 kb on - strand at 315.501 kb on - strand at 315.501 kb on - strand at 315.502 kb on - strand at 315.519 kb on + strand at 315.519 kb on + strand at 315.519 kb on - strand at 315.527 kb on - strand at 315.527 kb on - strand at 315.527 kb on - strand at 315.534 kb on + strand at 315.542 kb on - strand at 315.547 kb on + strand at 315.555 kb on - strand at 315.564 kb on - strand at 315.568 kb on + strand at 315.570 kb on - strand at 315.570 kb on - strand at 315.576 kb on - strand at 315.576 kb on - strand at 315.576 kb on - strand at 315.581 kb on + strand at 315.586 kb on + strand at 315.586 kb on + strand at 315.594 kb on - strand at 315.637 kb on + strand at 315.645 kb on - strand at 315.654 kb on - strand at 315.694 kb on + strand at 315.702 kb on - strand, within Synpcc7942_0318 at 315.711 kb on - strand, within Synpcc7942_0318 at 315.713 kb on + strand, within Synpcc7942_0318 at 315.745 kb on - strand, within Synpcc7942_0318 at 315.809 kb on + strand, within Synpcc7942_0318 at 315.856 kb on + strand, within Synpcc7942_0318 at 315.884 kb on - strand, within Synpcc7942_0318 at 315.889 kb on - strand, within Synpcc7942_0318 at 315.909 kb on + strand, within Synpcc7942_0318 at 315.915 kb on + strand, within Synpcc7942_0318 at 315.915 kb on + strand, within Synpcc7942_0318 at 315.959 kb on + strand, within Synpcc7942_0318 at 315.993 kb on + strand, within Synpcc7942_0318 at 315.993 kb on + strand, within Synpcc7942_0318 at 315.993 kb on + strand, within Synpcc7942_0318 at 316.038 kb on - strand, within Synpcc7942_0318 at 316.053 kb on - strand at 316.055 kb on + strand at 316.080 kb on + strand at 316.080 kb on + strand at 316.080 kb on + strand at 316.080 kb on + strand at 316.080 kb on + strand at 316.088 kb on - strand at 316.088 kb on - strand at 316.088 kb on - strand at 316.093 kb on + strand at 316.103 kb on + strand at 316.103 kb on + strand at 316.106 kb on - strand at 316.111 kb on - strand at 316.111 kb on - strand at 316.111 kb on - strand at 316.125 kb on + strand at 316.141 kb on + strand at 316.149 kb on - strand at 316.159 kb on - strand at 316.165 kb on + strand at 316.171 kb on - strand at 316.173 kb on - strand at 316.184 kb on - strand at 316.196 kb on + strand at 316.203 kb on + strand at 316.204 kb on - strand at 316.231 kb on + strand at 316.233 kb on - strand at 316.254 kb on - strand, within Synpcc7942_0319 at 316.269 kb on - strand, within Synpcc7942_0319 at 316.297 kb on - strand, within Synpcc7942_0319 at 316.340 kb on - strand, within Synpcc7942_0319
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 314,438 - -0.4 314,478 + digD Synpcc7942_0316 0.24 +1.3 314,484 + digD Synpcc7942_0316 0.27 +2.1 314,486 - digD Synpcc7942_0316 0.28 +0.9 314,486 - digD Synpcc7942_0316 0.28 -1.7 314,486 - digD Synpcc7942_0316 0.28 -0.2 314,491 - digD Synpcc7942_0316 0.31 +2.4 314,497 + digD Synpcc7942_0316 0.34 -1.6 314,505 - digD Synpcc7942_0316 0.38 +0.8 314,536 - digD Synpcc7942_0316 0.54 -0.6 314,538 + digD Synpcc7942_0316 0.55 +0.5 314,550 + digD Synpcc7942_0316 0.61 -1.8 314,558 - digD Synpcc7942_0316 0.66 +0.1 314,562 + digD Synpcc7942_0316 0.68 -1.8 314,562 + digD Synpcc7942_0316 0.68 +0.8 314,562 + digD Synpcc7942_0316 0.68 +0.9 314,570 - digD Synpcc7942_0316 0.72 -1.5 314,570 - digD Synpcc7942_0316 0.72 -0.6 314,580 - +0.1 314,625 + -1.2 314,647 + -0.2 314,647 + +1.7 314,647 + +1.7 314,647 + +1.3 314,655 - -1.9 314,660 - -1.7 314,666 - -1.8 314,666 - +2.4 314,758 - +4.1 314,770 - -0.9 314,795 + -1.8 314,795 + -0.2 314,803 - +2.0 314,824 - +1.9 314,848 + -3.1 314,872 - -0.1 314,891 + +2.5 314,892 + +1.1 314,896 + -1.5 314,908 + -1.1 314,928 + +1.3 314,941 + +0.3 314,946 + Synpcc7942_0317 0.11 +1.1 314,946 + Synpcc7942_0317 0.11 -1.5 314,949 - Synpcc7942_0317 0.11 -1.2 314,949 - Synpcc7942_0317 0.11 +1.6 314,954 - Synpcc7942_0317 0.12 +0.9 314,971 + Synpcc7942_0317 0.16 +7.3 314,979 - -1.0 314,995 + Synpcc7942_0317 0.21 -1.5 315,042 + Synpcc7942_0317 0.32 +0.4 315,042 + Synpcc7942_0317 0.32 +0.1 315,086 - Synpcc7942_0317 0.41 -2.2 315,095 + Synpcc7942_0317 0.43 +1.6 315,096 - Synpcc7942_0317 0.43 -0.3 315,103 - Synpcc7942_0317 0.45 +0.2 315,126 + Synpcc7942_0317 0.50 -0.4 315,138 + Synpcc7942_0317 0.53 +0.4 315,142 + Synpcc7942_0317 0.53 -2.5 315,142 - Synpcc7942_0317 0.53 -0.2 315,146 - Synpcc7942_0317 0.54 +1.4 315,150 - Synpcc7942_0317 0.55 -1.2 315,160 - Synpcc7942_0317 0.57 +1.4 315,189 + Synpcc7942_0317 0.64 +0.5 315,213 + Synpcc7942_0317 0.69 +2.2 315,221 - Synpcc7942_0317 0.71 -2.0 315,234 + Synpcc7942_0317 0.73 +0.6 315,242 - Synpcc7942_0317 0.75 +1.1 315,242 - Synpcc7942_0317 0.75 +2.6 315,254 - Synpcc7942_0317 0.78 +4.4 315,297 - Synpcc7942_0317 0.87 +1.1 315,302 + Synpcc7942_0317 0.88 +0.1 315,302 + Synpcc7942_0317 0.88 -2.0 315,318 + +2.0 315,322 + +2.4 315,323 + -0.9 315,324 + -1.8 315,332 - +1.1 315,332 - -0.4 315,354 - +1.8 315,413 - -1.9 315,418 - +1.5 315,423 - -0.9 315,433 - -0.5 315,442 + -1.1 315,458 - -2.7 315,485 + -2.5 315,493 + +1.1 315,493 - +1.1 315,501 - -0.2 315,501 - -1.3 315,502 - +0.5 315,519 + -1.3 315,519 + +1.9 315,519 - -0.9 315,527 - +0.9 315,527 - -2.0 315,527 - -0.3 315,534 + +2.0 315,542 - -1.2 315,547 + +2.2 315,555 - +1.6 315,564 - -0.6 315,568 + -2.6 315,570 - -2.0 315,570 - +0.5 315,576 - +1.6 315,576 - -0.9 315,576 - +0.4 315,581 + +0.9 315,586 + -1.4 315,586 + +2.4 315,594 - -3.0 315,637 + +1.4 315,645 - +0.4 315,654 - -0.1 315,694 + +3.1 315,702 - Synpcc7942_0318 0.13 +3.1 315,711 - Synpcc7942_0318 0.15 +0.3 315,713 + Synpcc7942_0318 0.16 -3.2 315,745 - Synpcc7942_0318 0.23 -1.3 315,809 + Synpcc7942_0318 0.37 -2.0 315,856 + Synpcc7942_0318 0.47 +1.9 315,884 - Synpcc7942_0318 0.53 -0.6 315,889 - Synpcc7942_0318 0.54 -2.5 315,909 + Synpcc7942_0318 0.59 -1.6 315,915 + Synpcc7942_0318 0.60 -1.8 315,915 + Synpcc7942_0318 0.60 +2.3 315,959 + Synpcc7942_0318 0.70 +0.5 315,993 + Synpcc7942_0318 0.77 -2.0 315,993 + Synpcc7942_0318 0.77 +2.1 315,993 + Synpcc7942_0318 0.77 -0.4 316,038 - Synpcc7942_0318 0.87 -2.6 316,053 - -0.3 316,055 + -0.6 316,080 + -1.5 316,080 + -1.5 316,080 + +1.5 316,080 + -3.2 316,080 + -0.5 316,088 - -1.3 316,088 - +2.0 316,088 - -0.7 316,093 + +1.1 316,103 + -2.1 316,103 + +0.5 316,106 - -0.3 316,111 - +2.0 316,111 - -2.0 316,111 - +1.6 316,125 + -1.3 316,141 + -0.7 316,149 - -0.5 316,159 - +4.2 316,165 + +0.1 316,171 - +2.9 316,173 - +0.3 316,184 - -4.6 316,196 + -1.8 316,203 + -1.2 316,204 - -2.8 316,231 + -2.6 316,233 - -1.5 316,254 - Synpcc7942_0319 0.11 -0.7 316,269 - Synpcc7942_0319 0.13 -2.9 316,297 - Synpcc7942_0319 0.16 -1.9 316,340 - Synpcc7942_0319 0.22 -0.9
Or see this region's nucleotide sequence