Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ispE and ksgA overlap by 4 nucleotides ksgA and Synpcc7942_0312 are separated by 26 nucleotides Synpcc7942_0312 and Synpcc7942_0313 are separated by 53 nucleotides
Synpcc7942_0310: ispE - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 309,473 to 310,417
ispE
Synpcc7942_0311: ksgA - dimethyladenosine transferase, at 310,414 to 311,253
ksgA
Synpcc7942_0312: Synpcc7942_0312 - hypothetical protein, at 311,280 to 311,708
_0312
Synpcc7942_0313: Synpcc7942_0313 - hypothetical protein, at 311,762 to 312,274
_0313
Position (kb) 310
311
312 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 309.439 kb on + strand at 309.486 kb on - strand at 309.489 kb on - strand at 310.455 kb on + strand at 310.455 kb on + strand at 310.479 kb on + strand at 310.479 kb on + strand at 310.519 kb on + strand, within ksgA at 310.553 kb on + strand at 310.555 kb on + strand, within ksgA at 310.555 kb on + strand, within ksgA at 310.561 kb on - strand, within ksgA at 310.563 kb on - strand, within ksgA at 310.563 kb on - strand at 310.568 kb on - strand, within ksgA at 310.573 kb on - strand at 310.632 kb on + strand, within ksgA at 310.632 kb on + strand, within ksgA at 310.640 kb on - strand at 310.650 kb on + strand at 310.662 kb on + strand, within ksgA at 310.694 kb on + strand, within ksgA at 310.722 kb on - strand, within ksgA at 310.738 kb on - strand at 310.750 kb on + strand, within ksgA at 310.751 kb on + strand, within ksgA at 310.751 kb on + strand at 310.794 kb on + strand, within ksgA at 310.800 kb on + strand at 310.802 kb on - strand, within ksgA at 310.802 kb on - strand at 310.916 kb on - strand, within ksgA at 310.951 kb on - strand, within ksgA at 311.009 kb on - strand at 311.034 kb on - strand, within ksgA at 311.103 kb on - strand, within ksgA at 311.135 kb on - strand, within ksgA at 311.210 kb on + strand at 311.215 kb on - strand at 311.226 kb on - strand at 311.238 kb on - strand at 311.244 kb on - strand at 311.260 kb on + strand at 311.263 kb on - strand at 311.263 kb on - strand at 311.263 kb on - strand at 311.301 kb on + strand at 311.301 kb on + strand at 311.307 kb on - strand at 311.307 kb on - strand at 311.308 kb on + strand at 311.330 kb on + strand, within Synpcc7942_0312 at 311.330 kb on + strand, within Synpcc7942_0312 at 311.330 kb on - strand, within Synpcc7942_0312 at 311.338 kb on - strand, within Synpcc7942_0312 at 311.338 kb on - strand, within Synpcc7942_0312 at 311.340 kb on + strand, within Synpcc7942_0312 at 311.348 kb on - strand, within Synpcc7942_0312 at 311.348 kb on - strand, within Synpcc7942_0312 at 311.369 kb on + strand at 311.374 kb on + strand, within Synpcc7942_0312 at 311.380 kb on + strand, within Synpcc7942_0312 at 311.384 kb on - strand, within Synpcc7942_0312 at 311.397 kb on + strand, within Synpcc7942_0312 at 311.416 kb on - strand, within Synpcc7942_0312 at 311.429 kb on - strand, within Synpcc7942_0312 at 311.438 kb on + strand, within Synpcc7942_0312 at 311.438 kb on + strand, within Synpcc7942_0312 at 311.471 kb on + strand, within Synpcc7942_0312 at 311.471 kb on + strand, within Synpcc7942_0312 at 311.479 kb on - strand, within Synpcc7942_0312 at 311.492 kb on - strand, within Synpcc7942_0312 at 311.498 kb on - strand, within Synpcc7942_0312 at 311.513 kb on - strand, within Synpcc7942_0312 at 311.519 kb on + strand, within Synpcc7942_0312 at 311.527 kb on - strand, within Synpcc7942_0312 at 311.552 kb on + strand, within Synpcc7942_0312 at 311.571 kb on + strand, within Synpcc7942_0312 at 311.588 kb on + strand, within Synpcc7942_0312 at 311.611 kb on + strand, within Synpcc7942_0312 at 311.621 kb on + strand, within Synpcc7942_0312 at 311.661 kb on + strand, within Synpcc7942_0312 at 311.667 kb on + strand at 311.718 kb on + strand at 311.719 kb on - strand at 311.726 kb on + strand at 311.733 kb on + strand at 311.741 kb on - strand at 311.763 kb on - strand at 311.795 kb on - strand at 311.811 kb on + strand at 311.839 kb on + strand, within Synpcc7942_0313 at 311.839 kb on + strand, within Synpcc7942_0313 at 311.842 kb on - strand, within Synpcc7942_0313 at 311.850 kb on + strand, within Synpcc7942_0313 at 311.924 kb on - strand, within Synpcc7942_0313 at 311.974 kb on - strand, within Synpcc7942_0313 at 311.981 kb on + strand, within Synpcc7942_0313 at 311.985 kb on + strand, within Synpcc7942_0313 at 312.019 kb on - strand, within Synpcc7942_0313 at 312.036 kb on + strand, within Synpcc7942_0313 at 312.036 kb on + strand, within Synpcc7942_0313 at 312.044 kb on - strand, within Synpcc7942_0313 at 312.049 kb on - strand, within Synpcc7942_0313 at 312.054 kb on - strand, within Synpcc7942_0313 at 312.060 kb on - strand, within Synpcc7942_0313 at 312.076 kb on + strand, within Synpcc7942_0313 at 312.081 kb on + strand, within Synpcc7942_0313 at 312.084 kb on - strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.156 kb on - strand, within Synpcc7942_0313 at 312.173 kb on + strand, within Synpcc7942_0313
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 309,439 + -1.3 309,486 - +3.4 309,489 - -0.9 310,455 + +0.6 310,455 + +0.5 310,479 + -2.0 310,479 + -3.3 310,519 + ksgA Synpcc7942_0311 0.12 -4.1 310,553 + -0.2 310,555 + ksgA Synpcc7942_0311 0.17 -2.5 310,555 + ksgA Synpcc7942_0311 0.17 -3.5 310,561 - ksgA Synpcc7942_0311 0.17 -1.3 310,563 - ksgA Synpcc7942_0311 0.18 -1.3 310,563 - -1.2 310,568 - ksgA Synpcc7942_0311 0.18 -1.6 310,573 - -1.0 310,632 + ksgA Synpcc7942_0311 0.26 -1.7 310,632 + ksgA Synpcc7942_0311 0.26 -2.8 310,640 - -3.2 310,650 + -1.8 310,662 + ksgA Synpcc7942_0311 0.30 -4.8 310,694 + ksgA Synpcc7942_0311 0.33 -2.6 310,722 - ksgA Synpcc7942_0311 0.37 -2.5 310,738 - -1.7 310,750 + ksgA Synpcc7942_0311 0.40 -3.4 310,751 + ksgA Synpcc7942_0311 0.40 -3.6 310,751 + -1.8 310,794 + ksgA Synpcc7942_0311 0.45 -2.3 310,800 + -2.3 310,802 - ksgA Synpcc7942_0311 0.46 -3.6 310,802 - -2.0 310,916 - ksgA Synpcc7942_0311 0.60 -3.3 310,951 - ksgA Synpcc7942_0311 0.64 -3.4 311,009 - -2.1 311,034 - ksgA Synpcc7942_0311 0.74 -1.2 311,103 - ksgA Synpcc7942_0311 0.82 -4.5 311,135 - ksgA Synpcc7942_0311 0.86 -4.9 311,210 + -3.1 311,215 - -2.8 311,226 - -2.5 311,238 - -2.9 311,244 - -0.2 311,260 + -0.5 311,263 - +0.9 311,263 - +1.3 311,263 - -1.7 311,301 + +3.6 311,301 + -2.8 311,307 - +0.0 311,307 - +2.3 311,308 + +1.7 311,330 + Synpcc7942_0312 0.12 -0.3 311,330 + Synpcc7942_0312 0.12 +3.0 311,330 - Synpcc7942_0312 0.12 -2.5 311,338 - Synpcc7942_0312 0.14 +1.3 311,338 - Synpcc7942_0312 0.14 -1.8 311,340 + Synpcc7942_0312 0.14 -0.3 311,348 - Synpcc7942_0312 0.16 -1.8 311,348 - Synpcc7942_0312 0.16 +1.3 311,369 + +0.5 311,374 + Synpcc7942_0312 0.22 -2.6 311,380 + Synpcc7942_0312 0.23 -0.5 311,384 - Synpcc7942_0312 0.24 +0.8 311,397 + Synpcc7942_0312 0.27 +0.4 311,416 - Synpcc7942_0312 0.32 -1.6 311,429 - Synpcc7942_0312 0.35 +1.4 311,438 + Synpcc7942_0312 0.37 +0.5 311,438 + Synpcc7942_0312 0.37 +0.6 311,471 + Synpcc7942_0312 0.45 -0.7 311,471 + Synpcc7942_0312 0.45 -0.0 311,479 - Synpcc7942_0312 0.46 -1.0 311,492 - Synpcc7942_0312 0.49 -1.2 311,498 - Synpcc7942_0312 0.51 +0.4 311,513 - Synpcc7942_0312 0.54 -1.2 311,519 + Synpcc7942_0312 0.56 -0.7 311,527 - Synpcc7942_0312 0.58 -1.0 311,552 + Synpcc7942_0312 0.63 -0.5 311,571 + Synpcc7942_0312 0.68 -2.5 311,588 + Synpcc7942_0312 0.72 -2.2 311,611 + Synpcc7942_0312 0.77 -2.0 311,621 + Synpcc7942_0312 0.79 -0.5 311,661 + Synpcc7942_0312 0.89 +3.7 311,667 + +1.4 311,718 + +3.8 311,719 - -1.9 311,726 + +3.2 311,733 + -0.1 311,741 - +0.8 311,763 - +1.0 311,795 - +3.6 311,811 + +1.4 311,839 + Synpcc7942_0313 0.15 +1.6 311,839 + Synpcc7942_0313 0.15 +1.2 311,842 - Synpcc7942_0313 0.16 -0.4 311,850 + Synpcc7942_0313 0.17 +3.9 311,924 - Synpcc7942_0313 0.32 +0.5 311,974 - Synpcc7942_0313 0.41 -0.6 311,981 + Synpcc7942_0313 0.43 +0.2 311,985 + Synpcc7942_0313 0.43 +0.6 312,019 - Synpcc7942_0313 0.50 -0.4 312,036 + Synpcc7942_0313 0.53 +0.4 312,036 + Synpcc7942_0313 0.53 +1.9 312,044 - Synpcc7942_0313 0.55 -2.1 312,049 - Synpcc7942_0313 0.56 -0.1 312,054 - Synpcc7942_0313 0.57 +1.3 312,060 - Synpcc7942_0313 0.58 +1.6 312,076 + Synpcc7942_0313 0.61 -0.7 312,081 + Synpcc7942_0313 0.62 -0.7 312,084 - Synpcc7942_0313 0.63 +0.8 312,133 + Synpcc7942_0313 0.72 -2.6 312,133 + Synpcc7942_0313 0.72 +1.9 312,133 + Synpcc7942_0313 0.72 -1.8 312,133 + Synpcc7942_0313 0.72 +0.5 312,141 - Synpcc7942_0313 0.74 -0.9 312,141 - Synpcc7942_0313 0.74 -0.5 312,141 - Synpcc7942_0313 0.74 +2.8 312,141 - Synpcc7942_0313 0.74 -2.4 312,141 - Synpcc7942_0313 0.74 +0.7 312,141 - Synpcc7942_0313 0.74 +1.4 312,156 - Synpcc7942_0313 0.77 +3.4 312,173 + Synpcc7942_0313 0.80 +1.7
Or see this region's nucleotide sequence