Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaJ4 and gidB are separated by 561 nucleotides gidB and Synpcc7942_0268 overlap by 4 nucleotides Synpcc7942_0268 and Synpcc7942_0269 are separated by 11 nucleotides
Synpcc7942_0266: dnaJ4 - Heat shock protein DnaJ-like, at 262,638 to 263,195
dnaJ4
Synpcc7942_0267: gidB - glucose-inhibited division protein B, at 263,757 to 264,485
gidB
Synpcc7942_0268: Synpcc7942_0268 - hypothetical protein, at 264,482 to 264,964
_0268
Synpcc7942_0269: Synpcc7942_0269 - hypothetical protein, at 264,976 to 265,857
_0269
Position (kb) 263
264
265 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 262.789 kb on + strand, within dnaJ4 at 262.797 kb on - strand at 262.797 kb on - strand, within dnaJ4 at 262.799 kb on + strand, within dnaJ4 at 262.818 kb on - strand, within dnaJ4 at 262.827 kb on + strand, within dnaJ4 at 262.827 kb on + strand, within dnaJ4 at 262.827 kb on + strand, within dnaJ4 at 262.881 kb on - strand, within dnaJ4 at 262.897 kb on + strand, within dnaJ4 at 262.908 kb on + strand, within dnaJ4 at 262.927 kb on + strand, within dnaJ4 at 262.928 kb on + strand, within dnaJ4 at 262.936 kb on - strand at 262.941 kb on - strand, within dnaJ4 at 262.946 kb on - strand, within dnaJ4 at 262.963 kb on + strand, within dnaJ4 at 262.966 kb on - strand, within dnaJ4 at 262.971 kb on - strand, within dnaJ4 at 262.989 kb on + strand, within dnaJ4 at 263.011 kb on + strand, within dnaJ4 at 263.022 kb on - strand, within dnaJ4 at 263.024 kb on - strand, within dnaJ4 at 263.034 kb on - strand, within dnaJ4 at 263.034 kb on - strand, within dnaJ4 at 263.053 kb on + strand, within dnaJ4 at 263.068 kb on + strand, within dnaJ4 at 263.076 kb on - strand, within dnaJ4 at 263.076 kb on - strand, within dnaJ4 at 263.076 kb on - strand, within dnaJ4 at 263.102 kb on + strand, within dnaJ4 at 263.109 kb on - strand, within dnaJ4 at 263.109 kb on - strand, within dnaJ4 at 263.111 kb on + strand, within dnaJ4 at 263.116 kb on + strand, within dnaJ4 at 263.119 kb on - strand, within dnaJ4 at 263.119 kb on - strand, within dnaJ4 at 263.124 kb on - strand, within dnaJ4 at 263.124 kb on - strand, within dnaJ4 at 263.178 kb on - strand at 263.185 kb on - strand at 263.195 kb on - strand at 263.225 kb on + strand at 263.230 kb on + strand at 263.233 kb on - strand at 263.237 kb on - strand at 263.238 kb on - strand at 263.267 kb on + strand at 263.296 kb on + strand at 263.525 kb on - strand at 263.603 kb on + strand at 263.603 kb on + strand at 263.603 kb on + strand at 263.603 kb on + strand at 263.603 kb on + strand at 263.603 kb on + strand at 263.603 kb on + strand at 263.609 kb on - strand at 263.611 kb on - strand at 263.611 kb on - strand at 263.611 kb on - strand at 263.611 kb on - strand at 263.611 kb on - strand at 263.611 kb on - strand at 263.611 kb on - strand at 263.611 kb on - strand at 263.611 kb on - strand at 263.611 kb on - strand at 263.611 kb on - strand at 263.621 kb on + strand at 263.623 kb on - strand at 263.624 kb on - strand at 263.631 kb on + strand at 263.637 kb on + strand at 263.637 kb on + strand at 263.637 kb on + strand at 263.674 kb on + strand at 263.723 kb on + strand at 263.736 kb on + strand at 263.737 kb on + strand at 263.761 kb on + strand at 263.799 kb on + strand at 263.800 kb on + strand at 263.806 kb on - strand at 263.815 kb on + strand at 263.821 kb on - strand at 263.821 kb on - strand at 263.821 kb on - strand at 263.832 kb on + strand, within gidB at 263.847 kb on + strand, within gidB at 263.855 kb on - strand, within gidB at 263.896 kb on + strand, within gidB at 263.896 kb on + strand, within gidB at 263.939 kb on + strand, within gidB at 263.955 kb on + strand, within gidB at 264.013 kb on + strand, within gidB at 264.021 kb on - strand, within gidB at 264.041 kb on + strand, within gidB at 264.055 kb on - strand, within gidB at 264.060 kb on - strand, within gidB at 264.075 kb on - strand, within gidB at 264.100 kb on + strand, within gidB at 264.119 kb on + strand, within gidB at 264.119 kb on + strand, within gidB at 264.119 kb on - strand, within gidB at 264.153 kb on + strand, within gidB at 264.156 kb on + strand, within gidB at 264.186 kb on + strand, within gidB at 264.233 kb on + strand, within gidB at 264.234 kb on + strand, within gidB at 264.240 kb on - strand, within gidB at 264.244 kb on + strand, within gidB at 264.245 kb on - strand, within gidB at 264.246 kb on - strand, within gidB at 264.252 kb on - strand, within gidB at 264.278 kb on + strand, within gidB at 264.281 kb on - strand, within gidB at 264.283 kb on + strand, within gidB at 264.322 kb on + strand, within gidB at 264.330 kb on - strand, within gidB at 264.377 kb on + strand, within gidB at 264.383 kb on + strand, within gidB at 264.396 kb on - strand, within gidB at 264.401 kb on + strand, within gidB at 264.401 kb on + strand, within gidB at 264.401 kb on + strand, within gidB at 264.403 kb on + strand, within gidB at 264.413 kb on + strand at 264.436 kb on - strand at 264.459 kb on - strand at 264.466 kb on + strand at 264.474 kb on - strand at 264.497 kb on - strand at 264.509 kb on + strand at 264.522 kb on + strand at 264.529 kb on + strand at 264.535 kb on - strand, within Synpcc7942_0268 at 264.544 kb on + strand, within Synpcc7942_0268 at 264.553 kb on + strand, within Synpcc7942_0268 at 264.553 kb on + strand, within Synpcc7942_0268 at 264.579 kb on - strand, within Synpcc7942_0268 at 264.595 kb on - strand, within Synpcc7942_0268 at 264.609 kb on + strand, within Synpcc7942_0268 at 264.609 kb on + strand, within Synpcc7942_0268 at 264.617 kb on - strand, within Synpcc7942_0268 at 264.617 kb on - strand, within Synpcc7942_0268 at 264.622 kb on - strand, within Synpcc7942_0268 at 264.624 kb on + strand, within Synpcc7942_0268 at 264.632 kb on - strand, within Synpcc7942_0268 at 264.652 kb on + strand, within Synpcc7942_0268 at 264.652 kb on + strand, within Synpcc7942_0268 at 264.660 kb on - strand, within Synpcc7942_0268 at 264.680 kb on + strand, within Synpcc7942_0268 at 264.695 kb on + strand, within Synpcc7942_0268 at 264.715 kb on + strand, within Synpcc7942_0268 at 264.762 kb on - strand, within Synpcc7942_0268 at 264.794 kb on - strand, within Synpcc7942_0268 at 264.813 kb on + strand, within Synpcc7942_0268 at 264.821 kb on - strand, within Synpcc7942_0268 at 264.835 kb on + strand, within Synpcc7942_0268 at 264.835 kb on + strand, within Synpcc7942_0268 at 264.839 kb on - strand, within Synpcc7942_0268 at 264.849 kb on + strand, within Synpcc7942_0268 at 264.857 kb on - strand, within Synpcc7942_0268 at 264.857 kb on - strand, within Synpcc7942_0268 at 264.859 kb on + strand, within Synpcc7942_0268 at 264.871 kb on + strand, within Synpcc7942_0268 at 264.872 kb on + strand, within Synpcc7942_0268 at 264.872 kb on + strand, within Synpcc7942_0268 at 264.872 kb on + strand, within Synpcc7942_0268 at 264.872 kb on + strand, within Synpcc7942_0268 at 264.872 kb on + strand, within Synpcc7942_0268 at 264.872 kb on + strand, within Synpcc7942_0268 at 264.872 kb on + strand, within Synpcc7942_0268 at 264.880 kb on - strand, within Synpcc7942_0268 at 264.880 kb on - strand, within Synpcc7942_0268 at 264.880 kb on - strand, within Synpcc7942_0268 at 264.880 kb on - strand, within Synpcc7942_0268 at 264.880 kb on - strand, within Synpcc7942_0268 at 264.880 kb on - strand, within Synpcc7942_0268 at 264.880 kb on - strand, within Synpcc7942_0268 at 264.880 kb on - strand, within Synpcc7942_0268 at 264.880 kb on - strand, within Synpcc7942_0268 at 264.883 kb on + strand, within Synpcc7942_0268 at 264.883 kb on + strand, within Synpcc7942_0268 at 264.883 kb on + strand, within Synpcc7942_0268 at 264.888 kb on + strand, within Synpcc7942_0268 at 264.891 kb on - strand, within Synpcc7942_0268 at 264.891 kb on - strand, within Synpcc7942_0268 at 264.891 kb on - strand, within Synpcc7942_0268 at 264.891 kb on - strand, within Synpcc7942_0268 at 264.893 kb on + strand, within Synpcc7942_0268 at 264.922 kb on - strand at 264.940 kb on + strand at 264.940 kb on + strand at 264.940 kb on + strand at 264.948 kb on - strand at 264.968 kb on + strand at 265.081 kb on - strand, within Synpcc7942_0269 at 265.103 kb on + strand, within Synpcc7942_0269 at 265.119 kb on + strand, within Synpcc7942_0269 at 265.148 kb on - strand, within Synpcc7942_0269 at 265.148 kb on - strand, within Synpcc7942_0269 at 265.175 kb on - strand, within Synpcc7942_0269 at 265.209 kb on + strand, within Synpcc7942_0269 at 265.218 kb on + strand, within Synpcc7942_0269 at 265.226 kb on - strand, within Synpcc7942_0269 at 265.231 kb on + strand at 265.280 kb on + strand, within Synpcc7942_0269 at 265.287 kb on - strand, within Synpcc7942_0269 at 265.322 kb on + strand, within Synpcc7942_0269 at 265.330 kb on - strand, within Synpcc7942_0269 at 265.330 kb on - strand, within Synpcc7942_0269 at 265.330 kb on - strand, within Synpcc7942_0269 at 265.330 kb on - strand, within Synpcc7942_0269 at 265.371 kb on - strand, within Synpcc7942_0269 at 265.377 kb on - strand, within Synpcc7942_0269 at 265.385 kb on + strand, within Synpcc7942_0269 at 265.395 kb on + strand, within Synpcc7942_0269 at 265.428 kb on + strand, within Synpcc7942_0269 at 265.436 kb on - strand, within Synpcc7942_0269 at 265.455 kb on + strand, within Synpcc7942_0269 at 265.463 kb on - strand, within Synpcc7942_0269 at 265.473 kb on + strand, within Synpcc7942_0269 at 265.478 kb on - strand, within Synpcc7942_0269
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 262,789 + dnaJ4 Synpcc7942_0266 0.27 -1.2 262,797 - +5.6 262,797 - dnaJ4 Synpcc7942_0266 0.28 +4.9 262,799 + dnaJ4 Synpcc7942_0266 0.29 -0.0 262,818 - dnaJ4 Synpcc7942_0266 0.32 +0.1 262,827 + dnaJ4 Synpcc7942_0266 0.34 +1.3 262,827 + dnaJ4 Synpcc7942_0266 0.34 -0.3 262,827 + dnaJ4 Synpcc7942_0266 0.34 +0.7 262,881 - dnaJ4 Synpcc7942_0266 0.44 +0.5 262,897 + dnaJ4 Synpcc7942_0266 0.46 -1.8 262,908 + dnaJ4 Synpcc7942_0266 0.48 -1.7 262,927 + dnaJ4 Synpcc7942_0266 0.52 -0.2 262,928 + dnaJ4 Synpcc7942_0266 0.52 +0.3 262,936 - -1.2 262,941 - dnaJ4 Synpcc7942_0266 0.54 -1.5 262,946 - dnaJ4 Synpcc7942_0266 0.55 -0.1 262,963 + dnaJ4 Synpcc7942_0266 0.58 -1.2 262,966 - dnaJ4 Synpcc7942_0266 0.59 -2.5 262,971 - dnaJ4 Synpcc7942_0266 0.60 +0.5 262,989 + dnaJ4 Synpcc7942_0266 0.63 +0.6 263,011 + dnaJ4 Synpcc7942_0266 0.67 +1.9 263,022 - dnaJ4 Synpcc7942_0266 0.69 +0.2 263,024 - dnaJ4 Synpcc7942_0266 0.69 -0.3 263,034 - dnaJ4 Synpcc7942_0266 0.71 -1.7 263,034 - dnaJ4 Synpcc7942_0266 0.71 +0.8 263,053 + dnaJ4 Synpcc7942_0266 0.74 +0.1 263,068 + dnaJ4 Synpcc7942_0266 0.77 +0.1 263,076 - dnaJ4 Synpcc7942_0266 0.78 -2.7 263,076 - dnaJ4 Synpcc7942_0266 0.78 -0.4 263,076 - dnaJ4 Synpcc7942_0266 0.78 -0.6 263,102 + dnaJ4 Synpcc7942_0266 0.83 +4.0 263,109 - dnaJ4 Synpcc7942_0266 0.84 +3.3 263,109 - dnaJ4 Synpcc7942_0266 0.84 +1.0 263,111 + dnaJ4 Synpcc7942_0266 0.85 -2.6 263,116 + dnaJ4 Synpcc7942_0266 0.86 +0.2 263,119 - dnaJ4 Synpcc7942_0266 0.86 +1.2 263,119 - dnaJ4 Synpcc7942_0266 0.86 -1.4 263,124 - dnaJ4 Synpcc7942_0266 0.87 +1.8 263,124 - dnaJ4 Synpcc7942_0266 0.87 +0.3 263,178 - +2.7 263,185 - -1.5 263,195 - -2.3 263,225 + +0.8 263,230 + +1.0 263,233 - +0.3 263,237 - +1.7 263,238 - +1.6 263,267 + -0.2 263,296 + +1.7 263,525 - +0.5 263,603 + +0.3 263,603 + -0.8 263,603 + +0.8 263,603 + -0.7 263,603 + -0.1 263,603 + -0.8 263,603 + -0.4 263,609 - -2.5 263,611 - -2.0 263,611 - +3.0 263,611 - -0.5 263,611 - -1.7 263,611 - +0.9 263,611 - +1.4 263,611 - -1.1 263,611 - +1.1 263,611 - +0.6 263,611 - -1.2 263,611 - -0.6 263,621 + +0.2 263,623 - -0.5 263,624 - +0.6 263,631 + +1.5 263,637 + +2.7 263,637 + -3.1 263,637 + -0.1 263,674 + -2.9 263,723 + +3.3 263,736 + -1.1 263,737 + -3.1 263,761 + -3.3 263,799 + -4.2 263,800 + -2.2 263,806 - -3.2 263,815 + +0.5 263,821 - -3.2 263,821 - -2.2 263,821 - -0.9 263,832 + gidB Synpcc7942_0267 0.10 -2.4 263,847 + gidB Synpcc7942_0267 0.12 -0.9 263,855 - gidB Synpcc7942_0267 0.13 -1.1 263,896 + gidB Synpcc7942_0267 0.19 -2.6 263,896 + gidB Synpcc7942_0267 0.19 -3.6 263,939 + gidB Synpcc7942_0267 0.25 -3.3 263,955 + gidB Synpcc7942_0267 0.27 -2.0 264,013 + gidB Synpcc7942_0267 0.35 +0.1 264,021 - gidB Synpcc7942_0267 0.36 -1.2 264,041 + gidB Synpcc7942_0267 0.39 -3.7 264,055 - gidB Synpcc7942_0267 0.41 -3.8 264,060 - gidB Synpcc7942_0267 0.42 -0.2 264,075 - gidB Synpcc7942_0267 0.44 -4.2 264,100 + gidB Synpcc7942_0267 0.47 -1.5 264,119 + gidB Synpcc7942_0267 0.50 -1.1 264,119 + gidB Synpcc7942_0267 0.50 -3.1 264,119 - gidB Synpcc7942_0267 0.50 -3.7 264,153 + gidB Synpcc7942_0267 0.54 -3.3 264,156 + gidB Synpcc7942_0267 0.55 -2.1 264,186 + gidB Synpcc7942_0267 0.59 -1.0 264,233 + gidB Synpcc7942_0267 0.65 -3.8 264,234 + gidB Synpcc7942_0267 0.65 +0.5 264,240 - gidB Synpcc7942_0267 0.66 -1.5 264,244 + gidB Synpcc7942_0267 0.67 +0.1 264,245 - gidB Synpcc7942_0267 0.67 -2.9 264,246 - gidB Synpcc7942_0267 0.67 -3.3 264,252 - gidB Synpcc7942_0267 0.68 -1.1 264,278 + gidB Synpcc7942_0267 0.71 -1.2 264,281 - gidB Synpcc7942_0267 0.72 -0.7 264,283 + gidB Synpcc7942_0267 0.72 -0.9 264,322 + gidB Synpcc7942_0267 0.78 -3.5 264,330 - gidB Synpcc7942_0267 0.79 -0.6 264,377 + gidB Synpcc7942_0267 0.85 -2.6 264,383 + gidB Synpcc7942_0267 0.86 -4.0 264,396 - gidB Synpcc7942_0267 0.88 -0.2 264,401 + gidB Synpcc7942_0267 0.88 -1.4 264,401 + gidB Synpcc7942_0267 0.88 -6.1 264,401 + gidB Synpcc7942_0267 0.88 -2.6 264,403 + gidB Synpcc7942_0267 0.89 -3.2 264,413 + -1.4 264,436 - -0.2 264,459 - -3.1 264,466 + +2.0 264,474 - -2.8 264,497 - +1.6 264,509 + +1.2 264,522 + +2.8 264,529 + +0.6 264,535 - Synpcc7942_0268 0.11 -2.2 264,544 + Synpcc7942_0268 0.13 +2.2 264,553 + Synpcc7942_0268 0.15 +0.7 264,553 + Synpcc7942_0268 0.15 +0.1 264,579 - Synpcc7942_0268 0.20 -1.2 264,595 - Synpcc7942_0268 0.23 +1.8 264,609 + Synpcc7942_0268 0.26 +0.8 264,609 + Synpcc7942_0268 0.26 -0.7 264,617 - Synpcc7942_0268 0.28 -2.1 264,617 - Synpcc7942_0268 0.28 -1.3 264,622 - Synpcc7942_0268 0.29 +4.0 264,624 + Synpcc7942_0268 0.29 +1.1 264,632 - Synpcc7942_0268 0.31 -0.2 264,652 + Synpcc7942_0268 0.35 -2.2 264,652 + Synpcc7942_0268 0.35 -0.7 264,660 - Synpcc7942_0268 0.37 -0.2 264,680 + Synpcc7942_0268 0.41 +1.4 264,695 + Synpcc7942_0268 0.44 -1.3 264,715 + Synpcc7942_0268 0.48 -0.6 264,762 - Synpcc7942_0268 0.58 -0.4 264,794 - Synpcc7942_0268 0.65 -0.7 264,813 + Synpcc7942_0268 0.69 +0.8 264,821 - Synpcc7942_0268 0.70 -1.8 264,835 + Synpcc7942_0268 0.73 +0.8 264,835 + Synpcc7942_0268 0.73 -0.6 264,839 - Synpcc7942_0268 0.74 +3.7 264,849 + Synpcc7942_0268 0.76 +0.8 264,857 - Synpcc7942_0268 0.78 -0.5 264,857 - Synpcc7942_0268 0.78 +0.2 264,859 + Synpcc7942_0268 0.78 -1.7 264,871 + Synpcc7942_0268 0.81 -1.5 264,872 + Synpcc7942_0268 0.81 +1.6 264,872 + Synpcc7942_0268 0.81 -0.1 264,872 + Synpcc7942_0268 0.81 +2.0 264,872 + Synpcc7942_0268 0.81 -1.0 264,872 + Synpcc7942_0268 0.81 +2.6 264,872 + Synpcc7942_0268 0.81 -2.2 264,872 + Synpcc7942_0268 0.81 +0.9 264,880 - Synpcc7942_0268 0.82 +1.7 264,880 - Synpcc7942_0268 0.82 +2.9 264,880 - Synpcc7942_0268 0.82 -0.7 264,880 - Synpcc7942_0268 0.82 -0.5 264,880 - Synpcc7942_0268 0.82 +3.1 264,880 - Synpcc7942_0268 0.82 +1.1 264,880 - Synpcc7942_0268 0.82 -1.6 264,880 - Synpcc7942_0268 0.82 +3.1 264,880 - Synpcc7942_0268 0.82 -0.6 264,883 + Synpcc7942_0268 0.83 -0.7 264,883 + Synpcc7942_0268 0.83 +0.2 264,883 + Synpcc7942_0268 0.83 -1.1 264,888 + Synpcc7942_0268 0.84 -1.9 264,891 - Synpcc7942_0268 0.85 -1.8 264,891 - Synpcc7942_0268 0.85 -0.4 264,891 - Synpcc7942_0268 0.85 -0.7 264,891 - Synpcc7942_0268 0.85 -0.0 264,893 + Synpcc7942_0268 0.85 +0.9 264,922 - -0.2 264,940 + -1.4 264,940 + -2.5 264,940 + +1.7 264,948 - -0.5 264,968 + -0.4 265,081 - Synpcc7942_0269 0.12 -1.3 265,103 + Synpcc7942_0269 0.14 -2.8 265,119 + Synpcc7942_0269 0.16 -2.2 265,148 - Synpcc7942_0269 0.20 +1.2 265,148 - Synpcc7942_0269 0.20 +0.2 265,175 - Synpcc7942_0269 0.23 +3.5 265,209 + Synpcc7942_0269 0.26 +0.2 265,218 + Synpcc7942_0269 0.27 +1.9 265,226 - Synpcc7942_0269 0.28 -0.5 265,231 + -1.7 265,280 + Synpcc7942_0269 0.34 -1.4 265,287 - Synpcc7942_0269 0.35 +0.4 265,322 + Synpcc7942_0269 0.39 +1.2 265,330 - Synpcc7942_0269 0.40 +1.6 265,330 - Synpcc7942_0269 0.40 -0.1 265,330 - Synpcc7942_0269 0.40 -0.5 265,330 - Synpcc7942_0269 0.40 -0.1 265,371 - Synpcc7942_0269 0.45 -1.6 265,377 - Synpcc7942_0269 0.45 +0.7 265,385 + Synpcc7942_0269 0.46 -0.7 265,395 + Synpcc7942_0269 0.48 -0.2 265,428 + Synpcc7942_0269 0.51 +5.2 265,436 - Synpcc7942_0269 0.52 +1.5 265,455 + Synpcc7942_0269 0.54 +0.1 265,463 - Synpcc7942_0269 0.55 +0.8 265,473 + Synpcc7942_0269 0.56 +2.8 265,478 - Synpcc7942_0269 0.57 -2.5
Or see this region's nucleotide sequence