Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0226 and pth overlap by 4 nucleotides pth and Synpcc7942_0228 are separated by 28 nucleotides Synpcc7942_0228 and Synpcc7942_0229 are separated by 113 nucleotides Synpcc7942_0229 and Synpcc7942_0230 are separated by 41 nucleotides
Synpcc7942_0226: Synpcc7942_0226 - Sec-independent protein translocase TatA, at 224,357 to 224,632
_0226
Synpcc7942_0227: pth - peptidyl-tRNA hydrolase, at 224,629 to 225,255
pth
Synpcc7942_0228: Synpcc7942_0228 - hypothetical protein, at 225,284 to 225,544
_0228
Synpcc7942_0229: Synpcc7942_0229 - hypothetical protein, at 225,658 to 226,221
_0229
Synpcc7942_0230: Synpcc7942_0230 - hypothetical protein, at 226,263 to 227,309
_0230
Position (kb)
225
226 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 224.347 kb on + strand at 224.626 kb on + strand at 224.626 kb on + strand at 224.634 kb on - strand at 224.634 kb on - strand at 225.183 kb on + strand at 225.227 kb on + strand at 225.235 kb on - strand at 225.235 kb on - strand at 225.249 kb on + strand at 225.257 kb on - strand at 225.257 kb on - strand at 225.257 kb on - strand at 225.261 kb on + strand at 225.261 kb on + strand at 225.264 kb on - strand at 225.275 kb on + strand at 225.279 kb on - strand at 225.311 kb on + strand, within Synpcc7942_0228 at 225.319 kb on - strand, within Synpcc7942_0228 at 225.337 kb on - strand, within Synpcc7942_0228 at 225.359 kb on + strand, within Synpcc7942_0228 at 225.378 kb on + strand, within Synpcc7942_0228 at 225.398 kb on - strand, within Synpcc7942_0228 at 225.423 kb on + strand, within Synpcc7942_0228 at 225.425 kb on + strand, within Synpcc7942_0228 at 225.464 kb on + strand, within Synpcc7942_0228 at 225.500 kb on + strand, within Synpcc7942_0228 at 225.527 kb on - strand at 225.560 kb on + strand at 225.572 kb on + strand at 225.580 kb on - strand at 225.617 kb on - strand at 225.630 kb on + strand at 225.630 kb on + strand at 225.630 kb on + strand at 225.638 kb on - strand at 225.676 kb on + strand at 225.701 kb on + strand at 225.721 kb on + strand, within Synpcc7942_0229 at 225.723 kb on + strand, within Synpcc7942_0229 at 225.804 kb on + strand, within Synpcc7942_0229 at 225.814 kb on - strand, within Synpcc7942_0229 at 225.821 kb on - strand, within Synpcc7942_0229 at 226.010 kb on - strand, within Synpcc7942_0229 at 226.020 kb on - strand, within Synpcc7942_0229 at 226.093 kb on + strand, within Synpcc7942_0229 at 226.107 kb on - strand, within Synpcc7942_0229 at 226.121 kb on - strand, within Synpcc7942_0229 at 226.121 kb on - strand, within Synpcc7942_0229 at 226.160 kb on + strand, within Synpcc7942_0229 at 226.168 kb on - strand at 226.168 kb on - strand at 226.181 kb on - strand at 226.190 kb on + strand at 226.190 kb on + strand at 226.237 kb on + strand at 226.237 kb on + strand at 226.243 kb on + strand at 226.268 kb on - strand at 226.325 kb on + strand at 226.333 kb on - strand at 226.366 kb on + strand at 226.366 kb on - strand at 226.367 kb on - strand at 226.374 kb on - strand, within Synpcc7942_0230 at 226.374 kb on - strand, within Synpcc7942_0230 at 226.385 kb on - strand, within Synpcc7942_0230 at 226.412 kb on - strand, within Synpcc7942_0230 at 226.416 kb on - strand, within Synpcc7942_0230 at 226.416 kb on - strand, within Synpcc7942_0230 at 226.473 kb on - strand, within Synpcc7942_0230 at 226.473 kb on - strand, within Synpcc7942_0230 at 226.484 kb on + strand, within Synpcc7942_0230 at 226.492 kb on - strand, within Synpcc7942_0230 at 226.492 kb on - strand, within Synpcc7942_0230 at 226.492 kb on - strand, within Synpcc7942_0230 at 226.507 kb on + strand, within Synpcc7942_0230 at 226.515 kb on - strand, within Synpcc7942_0230
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 224,347 + -0.3 224,626 + -2.2 224,626 + +0.5 224,634 - +0.9 224,634 - +2.1 225,183 + -2.8 225,227 + -1.3 225,235 - -0.1 225,235 - +0.0 225,249 + +2.4 225,257 - +0.4 225,257 - +4.5 225,257 - -2.9 225,261 + -1.3 225,261 + +0.0 225,264 - +0.0 225,275 + -0.8 225,279 - -0.5 225,311 + Synpcc7942_0228 0.10 +1.2 225,319 - Synpcc7942_0228 0.13 +1.5 225,337 - Synpcc7942_0228 0.20 -1.5 225,359 + Synpcc7942_0228 0.29 +1.5 225,378 + Synpcc7942_0228 0.36 -1.4 225,398 - Synpcc7942_0228 0.44 +5.3 225,423 + Synpcc7942_0228 0.53 +1.9 225,425 + Synpcc7942_0228 0.54 -0.4 225,464 + Synpcc7942_0228 0.69 +2.5 225,500 + Synpcc7942_0228 0.83 +0.8 225,527 - +0.9 225,560 + +1.0 225,572 + +0.2 225,580 - +0.4 225,617 - -0.6 225,630 + -0.1 225,630 + -1.9 225,630 + -1.3 225,638 - +0.6 225,676 + -1.0 225,701 + +0.3 225,721 + Synpcc7942_0229 0.11 +1.6 225,723 + Synpcc7942_0229 0.12 -2.1 225,804 + Synpcc7942_0229 0.26 +3.3 225,814 - Synpcc7942_0229 0.28 -0.3 225,821 - Synpcc7942_0229 0.29 -0.9 226,010 - Synpcc7942_0229 0.62 +3.5 226,020 - Synpcc7942_0229 0.64 +0.5 226,093 + Synpcc7942_0229 0.77 -1.9 226,107 - Synpcc7942_0229 0.80 +1.8 226,121 - Synpcc7942_0229 0.82 +0.8 226,121 - Synpcc7942_0229 0.82 +2.8 226,160 + Synpcc7942_0229 0.89 -3.5 226,168 - -2.8 226,168 - +1.9 226,181 - -1.5 226,190 + -1.3 226,190 + +4.6 226,237 + +1.0 226,237 + +1.9 226,243 + -2.3 226,268 - +0.6 226,325 + +0.3 226,333 - -0.3 226,366 + -0.5 226,366 - +1.8 226,367 - -0.5 226,374 - Synpcc7942_0230 0.11 -1.8 226,374 - Synpcc7942_0230 0.11 -0.6 226,385 - Synpcc7942_0230 0.12 +0.6 226,412 - Synpcc7942_0230 0.14 -2.2 226,416 - Synpcc7942_0230 0.15 -1.3 226,416 - Synpcc7942_0230 0.15 -0.8 226,473 - Synpcc7942_0230 0.20 +1.5 226,473 - Synpcc7942_0230 0.20 +1.1 226,484 + Synpcc7942_0230 0.21 -2.5 226,492 - Synpcc7942_0230 0.22 +1.8 226,492 - Synpcc7942_0230 0.22 -0.5 226,492 - Synpcc7942_0230 0.22 +5.0 226,507 + Synpcc7942_0230 0.23 -0.5 226,515 - Synpcc7942_0230 0.24 +0.1
Or see this region's nucleotide sequence