Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnN and Synpcc7942_0170 are separated by 150 nucleotides Synpcc7942_0170 and cysM are separated by 44 nucleotides cysM and Synpcc7942_0172 overlap by 4 nucleotides Synpcc7942_0172 and cysQ overlap by 4 nucleotides
Synpcc7942_0169: glnN - glutamate-ammonia ligase, glutamine synthetase type III, at 168,176 to 170,347
glnN
Synpcc7942_0170: Synpcc7942_0170 - hypothetical protein, at 170,498 to 170,752
_0170
Synpcc7942_0171: cysM - cysteine synthase A, at 170,797 to 171,915
cysM
Synpcc7942_0172: Synpcc7942_0172 - hypothetical protein, at 171,912 to 172,205
_0172
Synpcc7942_0173: cysQ - 3'-Phosphoadenosine 5'-phosphosulfate (PAPS) 3'-phosphatase-like, at 172,202 to 173,071
cysQ
Position (kb) 170
171
172 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 169.837 kb on + strand, within glnN at 169.845 kb on - strand, within glnN at 169.850 kb on - strand, within glnN at 169.860 kb on + strand, within glnN at 169.879 kb on + strand, within glnN at 169.887 kb on - strand, within glnN at 169.894 kb on - strand, within glnN at 169.904 kb on - strand, within glnN at 169.924 kb on + strand, within glnN at 169.965 kb on - strand, within glnN at 169.965 kb on - strand, within glnN at 169.977 kb on - strand, within glnN at 169.990 kb on + strand, within glnN at 170.053 kb on - strand at 170.073 kb on - strand, within glnN at 170.180 kb on + strand at 170.180 kb on + strand at 170.180 kb on + strand at 170.180 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.221 kb on + strand at 170.224 kb on - strand at 170.224 kb on - strand at 170.258 kb on + strand at 170.265 kb on - strand at 170.271 kb on - strand at 170.330 kb on + strand at 170.342 kb on + strand at 170.348 kb on + strand at 170.350 kb on - strand at 170.350 kb on - strand at 170.360 kb on + strand at 170.368 kb on - strand at 170.390 kb on + strand at 170.457 kb on - strand at 170.459 kb on - strand at 170.468 kb on - strand at 170.468 kb on - strand at 170.475 kb on - strand at 170.480 kb on - strand at 170.501 kb on - strand at 170.508 kb on + strand at 170.532 kb on + strand, within Synpcc7942_0170 at 170.566 kb on - strand, within Synpcc7942_0170 at 170.602 kb on - strand, within Synpcc7942_0170 at 170.646 kb on + strand, within Synpcc7942_0170 at 170.654 kb on - strand, within Synpcc7942_0170 at 170.656 kb on - strand, within Synpcc7942_0170 at 170.670 kb on - strand, within Synpcc7942_0170 at 170.672 kb on - strand, within Synpcc7942_0170 at 170.736 kb on + strand at 170.746 kb on + strand at 170.746 kb on + strand at 170.759 kb on + strand at 170.767 kb on - strand at 170.795 kb on - strand at 170.836 kb on + strand at 170.837 kb on + strand at 170.837 kb on + strand at 170.843 kb on + strand at 170.886 kb on - strand at 170.898 kb on + strand at 170.945 kb on + strand, within cysM at 170.988 kb on - strand, within cysM at 171.007 kb on + strand, within cysM at 171.161 kb on + strand, within cysM at 171.169 kb on - strand, within cysM at 171.174 kb on - strand, within cysM at 171.186 kb on + strand, within cysM at 171.189 kb on - strand, within cysM at 171.228 kb on + strand, within cysM at 171.244 kb on + strand, within cysM at 171.251 kb on + strand, within cysM at 171.274 kb on + strand, within cysM at 171.285 kb on + strand, within cysM at 171.293 kb on - strand, within cysM at 171.337 kb on + strand, within cysM at 171.361 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.559 kb on + strand at 171.559 kb on + strand, within cysM at 171.567 kb on - strand, within cysM at 171.567 kb on - strand, within cysM at 171.627 kb on - strand, within cysM at 171.687 kb on + strand, within cysM at 171.694 kb on + strand, within cysM at 171.694 kb on + strand, within cysM at 171.695 kb on - strand, within cysM at 171.707 kb on + strand, within cysM at 171.713 kb on + strand, within cysM at 171.725 kb on + strand, within cysM at 171.746 kb on + strand, within cysM at 171.858 kb on + strand at 171.866 kb on - strand at 171.874 kb on - strand at 171.884 kb on - strand at 171.959 kb on + strand, within Synpcc7942_0172 at 171.959 kb on - strand, within Synpcc7942_0172 at 171.965 kb on - strand, within Synpcc7942_0172 at 171.969 kb on + strand, within Synpcc7942_0172 at 172.008 kb on - strand, within Synpcc7942_0172 at 172.061 kb on - strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.118 kb on - strand, within Synpcc7942_0172 at 172.204 kb on + strand at 172.212 kb on - strand at 172.222 kb on - strand at 172.239 kb on - strand at 172.243 kb on + strand at 172.296 kb on - strand, within cysQ at 172.310 kb on - strand, within cysQ at 172.323 kb on + strand, within cysQ at 172.323 kb on + strand, within cysQ at 172.323 kb on - strand, within cysQ at 172.334 kb on + strand, within cysQ at 172.360 kb on + strand, within cysQ at 172.360 kb on + strand, within cysQ at 172.368 kb on - strand, within cysQ at 172.406 kb on + strand, within cysQ at 172.426 kb on - strand, within cysQ at 172.438 kb on + strand, within cysQ at 172.472 kb on + strand, within cysQ at 172.477 kb on + strand, within cysQ at 172.477 kb on + strand, within cysQ at 172.485 kb on - strand, within cysQ at 172.612 kb on - strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.666 kb on + strand, within cysQ at 172.698 kb on + strand, within cysQ at 172.704 kb on - strand, within cysQ at 172.733 kb on + strand, within cysQ at 172.776 kb on + strand, within cysQ at 172.808 kb on - strand, within cysQ at 172.813 kb on - strand, within cysQ at 172.813 kb on - strand at 172.815 kb on + strand, within cysQ at 172.830 kb on + strand, within cysQ
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 169,837 + glnN Synpcc7942_0169 0.76 +2.5 169,845 - glnN Synpcc7942_0169 0.77 +2.2 169,850 - glnN Synpcc7942_0169 0.77 -0.7 169,860 + glnN Synpcc7942_0169 0.78 +4.0 169,879 + glnN Synpcc7942_0169 0.78 +2.2 169,887 - glnN Synpcc7942_0169 0.79 -2.0 169,894 - glnN Synpcc7942_0169 0.79 -2.8 169,904 - glnN Synpcc7942_0169 0.80 +0.1 169,924 + glnN Synpcc7942_0169 0.80 +0.8 169,965 - glnN Synpcc7942_0169 0.82 +1.2 169,965 - glnN Synpcc7942_0169 0.82 +0.3 169,977 - glnN Synpcc7942_0169 0.83 -0.7 169,990 + glnN Synpcc7942_0169 0.84 +3.0 170,053 - -0.9 170,073 - glnN Synpcc7942_0169 0.87 -0.8 170,180 + -0.4 170,180 + +0.8 170,180 + +0.3 170,180 + +1.0 170,216 + +4.3 170,216 + -3.0 170,216 + +1.5 170,216 + +2.4 170,216 + +1.4 170,221 + +1.1 170,224 - +1.2 170,224 - +1.9 170,258 + +1.3 170,265 - -1.5 170,271 - +1.9 170,330 + -0.5 170,342 + -0.0 170,348 + -1.1 170,350 - +1.4 170,350 - +1.6 170,360 + -2.4 170,368 - -2.3 170,390 + -0.1 170,457 - +1.2 170,459 - +1.1 170,468 - +0.5 170,468 - +1.8 170,475 - -1.0 170,480 - -0.3 170,501 - -0.9 170,508 + +0.7 170,532 + Synpcc7942_0170 0.13 -0.7 170,566 - Synpcc7942_0170 0.27 -0.4 170,602 - Synpcc7942_0170 0.41 +4.0 170,646 + Synpcc7942_0170 0.58 -0.5 170,654 - Synpcc7942_0170 0.61 -2.1 170,656 - Synpcc7942_0170 0.62 -0.2 170,670 - Synpcc7942_0170 0.67 -1.6 170,672 - Synpcc7942_0170 0.68 -2.7 170,736 + +1.7 170,746 + -0.3 170,746 + +1.6 170,759 + -1.8 170,767 - -1.4 170,795 - +1.7 170,836 + -2.2 170,837 + +1.0 170,837 + +3.2 170,843 + -3.2 170,886 - -0.2 170,898 + -0.1 170,945 + cysM Synpcc7942_0171 0.13 -2.2 170,988 - cysM Synpcc7942_0171 0.17 -2.1 171,007 + cysM Synpcc7942_0171 0.19 -0.5 171,161 + cysM Synpcc7942_0171 0.33 -1.2 171,169 - cysM Synpcc7942_0171 0.33 -1.6 171,174 - cysM Synpcc7942_0171 0.34 -2.3 171,186 + cysM Synpcc7942_0171 0.35 -1.9 171,189 - cysM Synpcc7942_0171 0.35 -1.1 171,228 + cysM Synpcc7942_0171 0.39 -2.0 171,244 + cysM Synpcc7942_0171 0.40 -2.1 171,251 + cysM Synpcc7942_0171 0.41 -3.1 171,274 + cysM Synpcc7942_0171 0.43 -0.6 171,285 + cysM Synpcc7942_0171 0.44 -0.1 171,293 - cysM Synpcc7942_0171 0.44 -1.4 171,337 + cysM Synpcc7942_0171 0.48 -2.8 171,361 + cysM Synpcc7942_0171 0.50 -3.0 171,515 + cysM Synpcc7942_0171 0.64 -2.0 171,515 + cysM Synpcc7942_0171 0.64 -2.8 171,515 + cysM Synpcc7942_0171 0.64 +0.1 171,515 + -1.1 171,523 - cysM Synpcc7942_0171 0.65 -0.2 171,523 - cysM Synpcc7942_0171 0.65 -2.3 171,523 - cysM Synpcc7942_0171 0.65 -1.7 171,523 - cysM Synpcc7942_0171 0.65 -1.4 171,559 + -2.3 171,559 + cysM Synpcc7942_0171 0.68 -2.9 171,567 - cysM Synpcc7942_0171 0.69 -2.6 171,567 - cysM Synpcc7942_0171 0.69 -3.4 171,627 - cysM Synpcc7942_0171 0.74 -2.2 171,687 + cysM Synpcc7942_0171 0.80 -2.4 171,694 + cysM Synpcc7942_0171 0.80 -1.8 171,694 + cysM Synpcc7942_0171 0.80 +0.0 171,695 - cysM Synpcc7942_0171 0.80 -1.2 171,707 + cysM Synpcc7942_0171 0.81 -3.0 171,713 + cysM Synpcc7942_0171 0.82 -2.2 171,725 + cysM Synpcc7942_0171 0.83 -3.3 171,746 + cysM Synpcc7942_0171 0.85 -2.4 171,858 + +0.6 171,866 - -0.1 171,874 - -1.2 171,884 - -1.7 171,959 + Synpcc7942_0172 0.16 -1.5 171,959 - Synpcc7942_0172 0.16 -2.6 171,965 - Synpcc7942_0172 0.18 -1.7 171,969 + Synpcc7942_0172 0.19 +1.9 172,008 - Synpcc7942_0172 0.33 -0.9 172,061 - Synpcc7942_0172 0.51 -0.9 172,101 + Synpcc7942_0172 0.64 -0.9 172,101 + Synpcc7942_0172 0.64 -0.1 172,101 + Synpcc7942_0172 0.64 +0.8 172,118 - Synpcc7942_0172 0.70 -2.2 172,204 + -2.4 172,212 - -2.1 172,222 - -1.5 172,239 - +1.3 172,243 + +6.3 172,296 - cysQ Synpcc7942_0173 0.11 +1.7 172,310 - cysQ Synpcc7942_0173 0.12 -0.8 172,323 + cysQ Synpcc7942_0173 0.14 -3.4 172,323 + cysQ Synpcc7942_0173 0.14 +1.6 172,323 - cysQ Synpcc7942_0173 0.14 -1.1 172,334 + cysQ Synpcc7942_0173 0.15 +1.4 172,360 + cysQ Synpcc7942_0173 0.18 +3.3 172,360 + cysQ Synpcc7942_0173 0.18 +1.1 172,368 - cysQ Synpcc7942_0173 0.19 -0.2 172,406 + cysQ Synpcc7942_0173 0.23 -0.7 172,426 - cysQ Synpcc7942_0173 0.26 +2.9 172,438 + cysQ Synpcc7942_0173 0.27 +0.9 172,472 + cysQ Synpcc7942_0173 0.31 -1.7 172,477 + cysQ Synpcc7942_0173 0.32 -1.2 172,477 + cysQ Synpcc7942_0173 0.32 -2.3 172,485 - cysQ Synpcc7942_0173 0.33 +0.1 172,612 - cysQ Synpcc7942_0173 0.47 +0.8 172,625 + cysQ Synpcc7942_0173 0.49 -1.1 172,625 + cysQ Synpcc7942_0173 0.49 +0.7 172,625 + cysQ Synpcc7942_0173 0.49 -1.2 172,625 + -0.5 172,631 + cysQ Synpcc7942_0173 0.49 +0.9 172,631 + cysQ Synpcc7942_0173 0.49 -2.2 172,631 + cysQ Synpcc7942_0173 0.49 +2.3 172,631 + cysQ Synpcc7942_0173 0.49 -1.2 172,631 + cysQ Synpcc7942_0173 0.49 +1.8 172,631 + cysQ Synpcc7942_0173 0.49 +1.4 172,631 + +0.5 172,631 + cysQ Synpcc7942_0173 0.49 +0.5 172,631 + cysQ Synpcc7942_0173 0.49 +7.3 172,639 - cysQ Synpcc7942_0173 0.50 -1.3 172,639 - cysQ Synpcc7942_0173 0.50 -2.0 172,639 - cysQ Synpcc7942_0173 0.50 +0.7 172,639 - cysQ Synpcc7942_0173 0.50 -2.0 172,639 - cysQ Synpcc7942_0173 0.50 -0.1 172,639 - cysQ Synpcc7942_0173 0.50 +1.1 172,639 - cysQ Synpcc7942_0173 0.50 +1.7 172,639 - cysQ Synpcc7942_0173 0.50 -1.8 172,639 - cysQ Synpcc7942_0173 0.50 +1.9 172,666 + cysQ Synpcc7942_0173 0.53 -1.8 172,698 + cysQ Synpcc7942_0173 0.57 -1.4 172,704 - cysQ Synpcc7942_0173 0.58 -0.9 172,733 + cysQ Synpcc7942_0173 0.61 -0.7 172,776 + cysQ Synpcc7942_0173 0.66 +0.7 172,808 - cysQ Synpcc7942_0173 0.70 +1.2 172,813 - cysQ Synpcc7942_0173 0.70 -2.6 172,813 - +0.1 172,815 + cysQ Synpcc7942_0173 0.70 +0.0 172,830 + cysQ Synpcc7942_0173 0.72 -0.5
Or see this region's nucleotide sequence