Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt envM and ntcA are separated by 231 nucleotides ntcA and Synpcc7942_0128 are separated by 92 nucleotides Synpcc7942_0128 and Synpcc7942_0129 overlap by 4 nucleotides Synpcc7942_0129 and Synpcc7942_0130 overlap by 56 nucleotides
Synpcc7942_0126: envM - enoyl-(acyl carrier protein) reductase, at 125,382 to 126,158
envM
Synpcc7942_0127: ntcA - transcriptional regulator, Crp/Fnr family, at 126,390 to 127,058
ntcA
Synpcc7942_0128: Synpcc7942_0128 - hypothetical protein, at 127,151 to 128,449
_0128
Synpcc7942_0129: Synpcc7942_0129 - Resolvase, RNase H-like fold, at 128,446 to 128,877
_0129
Synpcc7942_0130: Synpcc7942_0130 - hypothetical protein, at 128,822 to 129,409
_0130
Position (kb)
127
128
129 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 126.259 kb on + strand at 126.267 kb on - strand at 126.329 kb on + strand at 126.334 kb on + strand at 126.342 kb on - strand at 126.362 kb on - strand at 126.369 kb on + strand at 126.369 kb on + strand at 126.369 kb on + strand at 126.371 kb on + strand at 126.377 kb on - strand at 127.053 kb on + strand at 127.061 kb on - strand at 127.093 kb on - strand at 127.116 kb on + strand at 127.116 kb on + strand at 127.116 kb on - strand at 127.118 kb on + strand at 127.123 kb on + strand at 127.123 kb on + strand at 127.131 kb on - strand at 127.131 kb on - strand at 127.131 kb on - strand at 127.131 kb on - strand at 127.171 kb on + strand at 127.276 kb on + strand at 127.278 kb on - strand at 127.355 kb on + strand, within Synpcc7942_0128 at 127.423 kb on + strand, within Synpcc7942_0128 at 127.423 kb on + strand, within Synpcc7942_0128 at 127.528 kb on - strand, within Synpcc7942_0128 at 127.642 kb on - strand, within Synpcc7942_0128 at 127.674 kb on + strand, within Synpcc7942_0128 at 127.692 kb on - strand, within Synpcc7942_0128 at 127.718 kb on + strand, within Synpcc7942_0128 at 127.745 kb on + strand, within Synpcc7942_0128 at 127.819 kb on + strand, within Synpcc7942_0128 at 127.847 kb on + strand, within Synpcc7942_0128 at 127.850 kb on - strand, within Synpcc7942_0128 at 127.888 kb on - strand, within Synpcc7942_0128 at 127.888 kb on - strand, within Synpcc7942_0128 at 127.898 kb on - strand, within Synpcc7942_0128 at 127.930 kb on - strand, within Synpcc7942_0128 at 127.959 kb on + strand, within Synpcc7942_0128 at 128.021 kb on + strand, within Synpcc7942_0128 at 128.171 kb on - strand, within Synpcc7942_0128 at 128.192 kb on - strand, within Synpcc7942_0128 at 128.213 kb on - strand, within Synpcc7942_0128 at 128.219 kb on - strand, within Synpcc7942_0128 at 128.221 kb on + strand, within Synpcc7942_0128 at 128.267 kb on + strand at 128.310 kb on + strand, within Synpcc7942_0128 at 128.326 kb on - strand at 128.381 kb on + strand at 128.429 kb on - strand at 128.443 kb on + strand at 128.443 kb on + strand at 128.443 kb on + strand at 128.443 kb on + strand at 128.443 kb on + strand at 128.451 kb on - strand at 128.451 kb on - strand at 128.451 kb on - strand at 128.451 kb on - strand at 128.453 kb on + strand at 128.461 kb on - strand at 128.471 kb on - strand at 128.482 kb on - strand at 128.611 kb on + strand, within Synpcc7942_0129 at 128.653 kb on + strand, within Synpcc7942_0129 at 128.659 kb on + strand, within Synpcc7942_0129 at 128.670 kb on + strand, within Synpcc7942_0129 at 128.711 kb on - strand, within Synpcc7942_0129 at 128.722 kb on - strand, within Synpcc7942_0129 at 128.750 kb on - strand, within Synpcc7942_0129 at 128.784 kb on - strand, within Synpcc7942_0129 at 128.843 kb on + strand at 128.907 kb on + strand, within Synpcc7942_0130 at 128.915 kb on - strand, within Synpcc7942_0130 at 128.920 kb on - strand, within Synpcc7942_0130 at 128.941 kb on - strand, within Synpcc7942_0130 at 128.977 kb on - strand, within Synpcc7942_0130 at 129.018 kb on - strand, within Synpcc7942_0130 at 129.031 kb on + strand, within Synpcc7942_0130 at 129.033 kb on + strand, within Synpcc7942_0130 at 129.051 kb on - strand, within Synpcc7942_0130 at 129.102 kb on + strand, within Synpcc7942_0130 at 129.122 kb on - strand, within Synpcc7942_0130 at 129.210 kb on - strand, within Synpcc7942_0130 at 129.224 kb on + strand, within Synpcc7942_0130 at 129.229 kb on + strand, within Synpcc7942_0130 at 129.231 kb on - strand, within Synpcc7942_0130 at 129.243 kb on + strand, within Synpcc7942_0130 at 129.251 kb on - strand, within Synpcc7942_0130 at 129.252 kb on + strand, within Synpcc7942_0130 at 129.323 kb on - strand, within Synpcc7942_0130 at 129.330 kb on - strand, within Synpcc7942_0130 at 129.332 kb on + strand, within Synpcc7942_0130 at 129.369 kb on - strand at 129.404 kb on + strand at 129.424 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 126,259 + -1.5 126,267 - -2.0 126,329 + -0.9 126,334 + -2.8 126,342 - -0.9 126,362 - +1.4 126,369 + -0.9 126,369 + +2.6 126,369 + -1.7 126,371 + -2.0 126,377 - +1.3 127,053 + +3.1 127,061 - +1.8 127,093 - -3.0 127,116 + -1.7 127,116 + -0.9 127,116 - -3.0 127,118 + -1.3 127,123 + +0.1 127,123 + -0.1 127,131 - +1.3 127,131 - -2.4 127,131 - -0.9 127,131 - -1.2 127,171 + -0.9 127,276 + -2.2 127,278 - -0.5 127,355 + Synpcc7942_0128 0.16 -1.7 127,423 + Synpcc7942_0128 0.21 -1.7 127,423 + Synpcc7942_0128 0.21 -2.8 127,528 - Synpcc7942_0128 0.29 -2.7 127,642 - Synpcc7942_0128 0.38 -1.2 127,674 + Synpcc7942_0128 0.40 -0.7 127,692 - Synpcc7942_0128 0.42 -0.2 127,718 + Synpcc7942_0128 0.44 -1.2 127,745 + Synpcc7942_0128 0.46 -1.1 127,819 + Synpcc7942_0128 0.51 -1.2 127,847 + Synpcc7942_0128 0.54 -3.3 127,850 - Synpcc7942_0128 0.54 -1.9 127,888 - Synpcc7942_0128 0.57 -0.7 127,888 - Synpcc7942_0128 0.57 -0.7 127,898 - Synpcc7942_0128 0.58 -0.9 127,930 - Synpcc7942_0128 0.60 -2.7 127,959 + Synpcc7942_0128 0.62 -2.4 128,021 + Synpcc7942_0128 0.67 -0.7 128,171 - Synpcc7942_0128 0.79 -4.6 128,192 - Synpcc7942_0128 0.80 -1.8 128,213 - Synpcc7942_0128 0.82 -1.5 128,219 - Synpcc7942_0128 0.82 -2.6 128,221 + Synpcc7942_0128 0.82 -1.1 128,267 + +1.1 128,310 + Synpcc7942_0128 0.89 -1.4 128,326 - -1.6 128,381 + -1.1 128,429 - -0.5 128,443 + +1.7 128,443 + -2.2 128,443 + -0.3 128,443 + -0.8 128,443 + +1.4 128,451 - +0.7 128,451 - +2.7 128,451 - -0.2 128,451 - +0.1 128,453 + -0.5 128,461 - -3.3 128,471 - +0.5 128,482 - -3.9 128,611 + Synpcc7942_0129 0.38 -0.2 128,653 + Synpcc7942_0129 0.48 +0.5 128,659 + Synpcc7942_0129 0.49 -0.9 128,670 + Synpcc7942_0129 0.52 -2.1 128,711 - Synpcc7942_0129 0.61 -2.7 128,722 - Synpcc7942_0129 0.64 -1.4 128,750 - Synpcc7942_0129 0.70 -1.4 128,784 - Synpcc7942_0129 0.78 +1.1 128,843 + -2.3 128,907 + Synpcc7942_0130 0.14 +0.3 128,915 - Synpcc7942_0130 0.16 +1.6 128,920 - Synpcc7942_0130 0.17 +0.4 128,941 - Synpcc7942_0130 0.20 +1.4 128,977 - Synpcc7942_0130 0.26 -0.6 129,018 - Synpcc7942_0130 0.33 +3.8 129,031 + Synpcc7942_0130 0.36 +0.5 129,033 + Synpcc7942_0130 0.36 -1.4 129,051 - Synpcc7942_0130 0.39 -0.2 129,102 + Synpcc7942_0130 0.48 +1.9 129,122 - Synpcc7942_0130 0.51 +5.1 129,210 - Synpcc7942_0130 0.66 +0.5 129,224 + Synpcc7942_0130 0.68 -1.1 129,229 + Synpcc7942_0130 0.69 +5.1 129,231 - Synpcc7942_0130 0.70 +0.2 129,243 + Synpcc7942_0130 0.72 +1.4 129,251 - Synpcc7942_0130 0.73 +0.3 129,252 + Synpcc7942_0130 0.73 -2.1 129,323 - Synpcc7942_0130 0.85 +0.5 129,330 - Synpcc7942_0130 0.86 +2.1 129,332 + Synpcc7942_0130 0.87 +3.0 129,369 - -1.6 129,404 + +3.8 129,424 - +3.6
Or see this region's nucleotide sequence