Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tmk and dnaX overlap by 1 nucleotides dnaX and rpaA overlap by 22 nucleotides rpaA and Synpcc7942_0096 are separated by 424 nucleotides
Synpcc7942_0093: tmk - Thymidylate kinase, at 91,029 to 91,676
tmk
Synpcc7942_0094: dnaX - DNA polymerase III subunit delta', at 91,676 to 92,611
dnaX
Synpcc7942_0095: rpaA - two component transcriptional regulator, winged helix family, at 92,590 to 93,339
rpaA
Synpcc7942_0096: Synpcc7942_0096 - hypothetical protein, at 93,764 to 94,285
_0096
Position (kb)
92
93
94 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 92.560 kb on + strand at 92.578 kb on - strand at 92.578 kb on - strand at 92.588 kb on - strand at 92.588 kb on - strand at 92.588 kb on - strand at 92.620 kb on + strand at 92.646 kb on + strand at 92.732 kb on - strand, within rpaA at 92.810 kb on - strand, within rpaA at 92.870 kb on + strand, within rpaA at 92.899 kb on - strand, within rpaA at 92.899 kb on - strand, within rpaA at 92.922 kb on - strand, within rpaA at 93.015 kb on - strand, within rpaA at 93.065 kb on + strand, within rpaA at 93.065 kb on + strand, within rpaA at 93.065 kb on + strand, within rpaA at 93.066 kb on - strand, within rpaA at 93.073 kb on - strand, within rpaA at 93.146 kb on - strand, within rpaA at 93.148 kb on - strand at 93.215 kb on + strand, within rpaA at 93.220 kb on + strand, within rpaA at 93.223 kb on - strand, within rpaA at 93.228 kb on + strand, within rpaA at 93.236 kb on - strand, within rpaA at 93.258 kb on - strand, within rpaA at 93.291 kb on + strand at 93.349 kb on - strand at 93.378 kb on - strand at 93.414 kb on + strand at 93.456 kb on + strand at 93.456 kb on + strand at 93.461 kb on + strand at 93.461 kb on + strand at 93.461 kb on + strand at 93.461 kb on + strand at 93.464 kb on - strand at 93.466 kb on + strand at 93.469 kb on - strand at 93.469 kb on - strand at 93.539 kb on + strand at 93.552 kb on - strand at 93.594 kb on + strand at 93.599 kb on + strand at 93.599 kb on + strand at 93.604 kb on + strand at 93.607 kb on - strand at 93.650 kb on + strand at 93.663 kb on + strand at 93.687 kb on - strand at 93.697 kb on - strand at 93.697 kb on - strand at 93.737 kb on + strand at 93.737 kb on + strand at 93.778 kb on + strand at 93.779 kb on + strand at 93.779 kb on + strand at 93.787 kb on - strand at 93.811 kb on - strand at 93.813 kb on + strand at 93.813 kb on + strand at 93.821 kb on - strand, within Synpcc7942_0096 at 93.821 kb on - strand, within Synpcc7942_0096 at 93.839 kb on + strand, within Synpcc7942_0096 at 93.901 kb on + strand, within Synpcc7942_0096 at 93.901 kb on + strand, within Synpcc7942_0096 at 93.901 kb on + strand, within Synpcc7942_0096 at 93.922 kb on + strand, within Synpcc7942_0096 at 93.932 kb on + strand, within Synpcc7942_0096 at 93.963 kb on - strand, within Synpcc7942_0096 at 94.009 kb on - strand, within Synpcc7942_0096 at 94.011 kb on + strand, within Synpcc7942_0096 at 94.018 kb on + strand, within Synpcc7942_0096 at 94.027 kb on + strand, within Synpcc7942_0096 at 94.027 kb on + strand, within Synpcc7942_0096 at 94.027 kb on + strand, within Synpcc7942_0096 at 94.027 kb on + strand, within Synpcc7942_0096 at 94.027 kb on + strand, within Synpcc7942_0096 at 94.032 kb on + strand, within Synpcc7942_0096 at 94.083 kb on - strand, within Synpcc7942_0096 at 94.153 kb on + strand, within Synpcc7942_0096
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 92,560 + +1.5 92,578 - +1.6 92,578 - -1.4 92,588 - -1.3 92,588 - +0.0 92,588 - -0.8 92,620 + -1.4 92,646 + -2.4 92,732 - rpaA Synpcc7942_0095 0.19 -1.1 92,810 - rpaA Synpcc7942_0095 0.29 +1.1 92,870 + rpaA Synpcc7942_0095 0.37 -2.2 92,899 - rpaA Synpcc7942_0095 0.41 -3.3 92,899 - rpaA Synpcc7942_0095 0.41 -1.2 92,922 - rpaA Synpcc7942_0095 0.44 +1.7 93,015 - rpaA Synpcc7942_0095 0.57 +1.1 93,065 + rpaA Synpcc7942_0095 0.63 +1.1 93,065 + rpaA Synpcc7942_0095 0.63 -1.2 93,065 + rpaA Synpcc7942_0095 0.63 -1.1 93,066 - rpaA Synpcc7942_0095 0.63 -1.2 93,073 - rpaA Synpcc7942_0095 0.64 -1.1 93,146 - rpaA Synpcc7942_0095 0.74 -2.6 93,148 - +3.5 93,215 + rpaA Synpcc7942_0095 0.83 +0.1 93,220 + rpaA Synpcc7942_0095 0.84 -0.2 93,223 - rpaA Synpcc7942_0095 0.84 -1.9 93,228 + rpaA Synpcc7942_0095 0.85 -3.0 93,236 - rpaA Synpcc7942_0095 0.86 -2.5 93,258 - rpaA Synpcc7942_0095 0.89 -1.0 93,291 + -1.1 93,349 - -2.6 93,378 - +2.7 93,414 + -0.7 93,456 + -2.0 93,456 + +1.4 93,461 + +1.7 93,461 + +3.6 93,461 + +2.4 93,461 + +4.0 93,464 - +1.1 93,466 + +2.3 93,469 - +2.3 93,469 - +1.8 93,539 + +1.0 93,552 - +1.5 93,594 + -2.3 93,599 + +1.9 93,599 + -0.5 93,604 + -0.2 93,607 - -0.1 93,650 + -0.1 93,663 + +1.2 93,687 - -0.8 93,697 - -1.5 93,697 - -0.7 93,737 + +0.1 93,737 + +0.3 93,778 + -1.6 93,779 + +3.0 93,779 + -1.5 93,787 - +2.3 93,811 - +3.6 93,813 + -0.3 93,813 + +1.2 93,821 - Synpcc7942_0096 0.11 -0.8 93,821 - Synpcc7942_0096 0.11 +2.7 93,839 + Synpcc7942_0096 0.14 -1.4 93,901 + Synpcc7942_0096 0.26 +1.2 93,901 + Synpcc7942_0096 0.26 -2.9 93,901 + Synpcc7942_0096 0.26 -1.1 93,922 + Synpcc7942_0096 0.30 +0.5 93,932 + Synpcc7942_0096 0.32 +0.2 93,963 - Synpcc7942_0096 0.38 +1.6 94,009 - Synpcc7942_0096 0.47 +1.8 94,011 + Synpcc7942_0096 0.47 -2.9 94,018 + Synpcc7942_0096 0.49 -0.7 94,027 + Synpcc7942_0096 0.50 +2.8 94,027 + Synpcc7942_0096 0.50 +0.3 94,027 + Synpcc7942_0096 0.50 +0.7 94,027 + Synpcc7942_0096 0.50 +2.5 94,027 + Synpcc7942_0096 0.50 -1.6 94,032 + Synpcc7942_0096 0.51 +1.5 94,083 - Synpcc7942_0096 0.61 +1.6 94,153 + Synpcc7942_0096 0.75 -2.0
Or see this region's nucleotide sequence