Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0090 and ycf21 overlap by 4 nucleotides ycf21 and Synpcc7942_0092 are separated by 256 nucleotides Synpcc7942_0092 and tmk are separated by 98 nucleotides tmk and dnaX overlap by 1 nucleotides
Synpcc7942_0090: Synpcc7942_0090 - transcriptional regulator, GntR family, at 88,735 to 89,718
_0090
Synpcc7942_0091: ycf21 - conserved hypothetical protein YCF21, at 89,715 to 90,326
ycf21
Synpcc7942_0092: Synpcc7942_0092 - hypothetical protein, at 90,583 to 90,930
_0092
Synpcc7942_0093: tmk - Thymidylate kinase, at 91,029 to 91,676
tmk
Synpcc7942_0094: dnaX - DNA polymerase III subunit delta', at 91,676 to 92,611
dnaX
Position (kb)
90
91 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 89.739 kb on - strand at 89.749 kb on - strand at 90.365 kb on - strand at 90.433 kb on + strand at 90.436 kb on - strand at 90.464 kb on + strand at 90.464 kb on + strand at 90.469 kb on + strand at 90.469 kb on + strand at 90.472 kb on - strand at 90.472 kb on - strand at 90.472 kb on - strand at 90.472 kb on - strand at 90.472 kb on - strand at 90.477 kb on - strand at 90.485 kb on + strand at 90.503 kb on + strand at 90.503 kb on + strand at 90.503 kb on + strand at 90.511 kb on - strand at 90.511 kb on - strand at 90.511 kb on - strand at 90.539 kb on - strand at 90.547 kb on - strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.560 kb on - strand at 90.560 kb on - strand at 90.560 kb on - strand at 90.560 kb on - strand at 90.560 kb on - strand at 90.575 kb on - strand at 90.580 kb on + strand at 90.583 kb on - strand at 90.588 kb on - strand at 90.588 kb on - strand at 90.588 kb on - strand at 90.666 kb on + strand, within Synpcc7942_0092 at 90.674 kb on - strand, within Synpcc7942_0092 at 90.674 kb on - strand, within Synpcc7942_0092 at 90.676 kb on + strand, within Synpcc7942_0092 at 90.807 kb on + strand, within Synpcc7942_0092 at 90.807 kb on + strand, within Synpcc7942_0092 at 90.817 kb on - strand, within Synpcc7942_0092 at 90.844 kb on - strand, within Synpcc7942_0092 at 90.852 kb on + strand, within Synpcc7942_0092 at 90.860 kb on - strand, within Synpcc7942_0092 at 90.865 kb on + strand, within Synpcc7942_0092 at 90.865 kb on + strand, within Synpcc7942_0092 at 90.870 kb on + strand, within Synpcc7942_0092 at 90.870 kb on + strand, within Synpcc7942_0092 at 90.875 kb on + strand, within Synpcc7942_0092 at 90.878 kb on - strand, within Synpcc7942_0092 at 90.878 kb on - strand, within Synpcc7942_0092 at 90.883 kb on - strand, within Synpcc7942_0092 at 90.889 kb on - strand, within Synpcc7942_0092 at 90.958 kb on + strand at 91.007 kb on - strand at 91.022 kb on - strand at 91.027 kb on + strand at 91.029 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 89,739 - +4.3 89,749 - +1.4 90,365 - +3.3 90,433 + +1.6 90,436 - -0.4 90,464 + -1.6 90,464 + +0.3 90,469 + +0.5 90,469 + -2.2 90,472 - -0.8 90,472 - -2.4 90,472 - -1.5 90,472 - +0.5 90,472 - +2.4 90,477 - -0.1 90,485 + -0.9 90,503 + -1.1 90,503 + -0.7 90,503 + -2.0 90,511 - -1.0 90,511 - +0.3 90,511 - -1.2 90,539 - +0.7 90,547 - +1.0 90,552 + -2.2 90,552 + -0.5 90,552 + -1.5 90,552 + -2.1 90,552 + -0.2 90,552 + +2.2 90,552 + +2.6 90,552 + -0.3 90,560 - -3.3 90,560 - -3.7 90,560 - +0.0 90,560 - -1.1 90,560 - +3.3 90,575 - -1.5 90,580 + +0.3 90,583 - -1.9 90,588 - -0.5 90,588 - -0.7 90,588 - +0.5 90,666 + Synpcc7942_0092 0.24 -2.3 90,674 - Synpcc7942_0092 0.26 -3.4 90,674 - Synpcc7942_0092 0.26 -1.9 90,676 + Synpcc7942_0092 0.27 -0.5 90,807 + Synpcc7942_0092 0.64 -2.0 90,807 + Synpcc7942_0092 0.64 -4.8 90,817 - Synpcc7942_0092 0.67 -2.0 90,844 - Synpcc7942_0092 0.75 -2.5 90,852 + Synpcc7942_0092 0.77 -1.9 90,860 - Synpcc7942_0092 0.80 -1.7 90,865 + Synpcc7942_0092 0.81 -4.0 90,865 + Synpcc7942_0092 0.81 -2.9 90,870 + Synpcc7942_0092 0.82 -0.7 90,870 + Synpcc7942_0092 0.82 -0.4 90,875 + Synpcc7942_0092 0.84 -1.5 90,878 - Synpcc7942_0092 0.85 -3.2 90,878 - Synpcc7942_0092 0.85 -0.7 90,883 - Synpcc7942_0092 0.86 -3.0 90,889 - Synpcc7942_0092 0.88 -2.1 90,958 + -3.0 91,007 - -3.5 91,022 - -3.0 91,027 + -2.7 91,029 - -3.5
Or see this region's nucleotide sequence