Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0083 and Synpcc7942_0084 overlap by 4 nucleotides Synpcc7942_0084 and Synpcc7942_0085 are separated by 260 nucleotides Synpcc7942_0085 and glgX are separated by 5 nucleotides
Synpcc7942_0083: Synpcc7942_0083 - hypothetical protein, at 81,023 to 81,220
_0083
Synpcc7942_0084: Synpcc7942_0084 - hypothetical protein, at 81,217 to 81,918
_0084
Synpcc7942_0085: Synpcc7942_0085 - HAD-superfamily hydrolase subfamily IA, variant 3, at 82,179 to 82,865
_0085
Synpcc7942_0086: glgX - isoamylase. Glycosyl Hydrolase family 13., at 82,871 to 84,955
glgX
Position (kb) 82
83 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 81.188 kb on + strand, within Synpcc7942_0083 at 81.194 kb on + strand, within Synpcc7942_0083 at 81.195 kb on + strand, within Synpcc7942_0083 at 81.196 kb on + strand, within Synpcc7942_0083 at 81.228 kb on + strand at 81.267 kb on + strand at 81.276 kb on - strand at 81.284 kb on + strand at 81.284 kb on + strand at 81.284 kb on + strand at 81.287 kb on - strand at 81.289 kb on + strand, within Synpcc7942_0084 at 81.289 kb on + strand, within Synpcc7942_0084 at 81.326 kb on + strand, within Synpcc7942_0084 at 81.334 kb on - strand, within Synpcc7942_0084 at 81.358 kb on - strand, within Synpcc7942_0084 at 81.368 kb on + strand, within Synpcc7942_0084 at 81.459 kb on + strand, within Synpcc7942_0084 at 81.459 kb on + strand, within Synpcc7942_0084 at 81.461 kb on + strand, within Synpcc7942_0084 at 81.467 kb on - strand, within Synpcc7942_0084 at 81.475 kb on + strand, within Synpcc7942_0084 at 81.483 kb on - strand, within Synpcc7942_0084 at 81.483 kb on - strand, within Synpcc7942_0084 at 81.488 kb on - strand, within Synpcc7942_0084 at 81.488 kb on - strand, within Synpcc7942_0084 at 81.488 kb on - strand, within Synpcc7942_0084 at 81.495 kb on + strand, within Synpcc7942_0084 at 81.495 kb on + strand, within Synpcc7942_0084 at 81.496 kb on + strand, within Synpcc7942_0084 at 81.503 kb on + strand, within Synpcc7942_0084 at 81.503 kb on - strand, within Synpcc7942_0084 at 81.504 kb on - strand, within Synpcc7942_0084 at 81.504 kb on - strand, within Synpcc7942_0084 at 81.535 kb on - strand, within Synpcc7942_0084 at 81.550 kb on + strand, within Synpcc7942_0084 at 81.558 kb on + strand, within Synpcc7942_0084 at 81.558 kb on - strand, within Synpcc7942_0084 at 81.566 kb on - strand, within Synpcc7942_0084 at 81.566 kb on - strand, within Synpcc7942_0084 at 81.584 kb on + strand, within Synpcc7942_0084 at 81.595 kb on + strand, within Synpcc7942_0084 at 81.619 kb on + strand, within Synpcc7942_0084 at 81.622 kb on - strand, within Synpcc7942_0084 at 81.624 kb on + strand, within Synpcc7942_0084 at 81.634 kb on + strand, within Synpcc7942_0084 at 81.644 kb on - strand, within Synpcc7942_0084 at 81.650 kb on - strand, within Synpcc7942_0084 at 81.691 kb on + strand, within Synpcc7942_0084 at 81.694 kb on - strand, within Synpcc7942_0084 at 81.754 kb on + strand, within Synpcc7942_0084 at 81.772 kb on + strand, within Synpcc7942_0084 at 81.772 kb on - strand, within Synpcc7942_0084 at 81.789 kb on + strand at 81.797 kb on - strand, within Synpcc7942_0084 at 81.808 kb on - strand, within Synpcc7942_0084 at 81.808 kb on - strand, within Synpcc7942_0084 at 81.854 kb on + strand at 81.854 kb on + strand at 81.854 kb on + strand at 81.854 kb on + strand at 81.857 kb on - strand at 81.862 kb on - strand at 81.864 kb on - strand at 81.918 kb on + strand at 81.925 kb on + strand at 81.925 kb on + strand at 81.925 kb on + strand at 81.925 kb on + strand at 81.925 kb on + strand at 81.925 kb on + strand at 81.925 kb on + strand at 81.933 kb on - strand at 81.933 kb on - strand at 81.945 kb on + strand at 81.984 kb on + strand at 81.984 kb on + strand at 81.996 kb on + strand at 81.998 kb on + strand at 82.004 kb on - strand at 82.004 kb on - strand at 82.004 kb on - strand at 82.004 kb on - strand at 82.004 kb on - strand at 82.004 kb on - strand at 82.017 kb on + strand at 82.029 kb on + strand at 82.040 kb on + strand at 82.086 kb on + strand at 82.123 kb on + strand at 82.123 kb on + strand at 82.147 kb on + strand at 82.147 kb on + strand at 82.147 kb on + strand at 82.147 kb on + strand at 82.148 kb on + strand at 82.148 kb on + strand at 82.148 kb on + strand at 82.155 kb on - strand at 82.161 kb on - strand at 82.170 kb on + strand at 82.170 kb on + strand at 82.178 kb on - strand at 82.183 kb on + strand at 82.183 kb on + strand at 82.183 kb on + strand at 82.185 kb on - strand at 82.191 kb on - strand at 82.195 kb on + strand at 82.195 kb on + strand at 82.203 kb on - strand at 82.227 kb on - strand at 82.293 kb on - strand, within Synpcc7942_0085 at 82.293 kb on - strand, within Synpcc7942_0085 at 82.296 kb on + strand, within Synpcc7942_0085 at 82.300 kb on + strand, within Synpcc7942_0085 at 82.373 kb on - strand, within Synpcc7942_0085 at 82.375 kb on + strand, within Synpcc7942_0085 at 82.380 kb on + strand, within Synpcc7942_0085 at 82.383 kb on - strand, within Synpcc7942_0085 at 82.429 kb on + strand, within Synpcc7942_0085 at 82.508 kb on + strand, within Synpcc7942_0085 at 82.516 kb on - strand, within Synpcc7942_0085 at 82.539 kb on - strand, within Synpcc7942_0085 at 82.541 kb on + strand, within Synpcc7942_0085 at 82.541 kb on + strand, within Synpcc7942_0085 at 82.541 kb on + strand, within Synpcc7942_0085 at 82.542 kb on - strand, within Synpcc7942_0085 at 82.546 kb on + strand, within Synpcc7942_0085 at 82.549 kb on - strand, within Synpcc7942_0085 at 82.552 kb on - strand, within Synpcc7942_0085 at 82.559 kb on + strand, within Synpcc7942_0085 at 82.559 kb on + strand, within Synpcc7942_0085 at 82.559 kb on + strand, within Synpcc7942_0085 at 82.570 kb on + strand, within Synpcc7942_0085 at 82.580 kb on + strand, within Synpcc7942_0085 at 82.679 kb on - strand, within Synpcc7942_0085 at 82.705 kb on + strand, within Synpcc7942_0085 at 82.705 kb on + strand, within Synpcc7942_0085 at 82.705 kb on + strand, within Synpcc7942_0085 at 82.713 kb on - strand, within Synpcc7942_0085 at 82.713 kb on - strand, within Synpcc7942_0085 at 82.717 kb on + strand, within Synpcc7942_0085 at 82.725 kb on - strand, within Synpcc7942_0085 at 82.725 kb on - strand, within Synpcc7942_0085 at 82.747 kb on - strand, within Synpcc7942_0085 at 82.889 kb on - strand at 82.914 kb on + strand at 82.922 kb on - strand at 82.982 kb on + strand at 82.983 kb on + strand at 82.991 kb on - strand at 83.041 kb on + strand at 83.043 kb on + strand at 83.055 kb on + strand at 83.055 kb on + strand at 83.096 kb on + strand, within glgX at 83.107 kb on + strand, within glgX at 83.128 kb on + strand, within glgX at 83.177 kb on - strand, within glgX at 83.198 kb on - strand, within glgX at 83.215 kb on + strand, within glgX at 83.250 kb on - strand, within glgX at 83.334 kb on + strand, within glgX at 83.342 kb on - strand, within glgX at 83.342 kb on - strand, within glgX at 83.391 kb on - strand, within glgX at 83.391 kb on - strand, within glgX at 83.393 kb on + strand at 83.405 kb on + strand at 83.408 kb on - strand, within glgX at 83.426 kb on + strand, within glgX at 83.520 kb on + strand, within glgX at 83.543 kb on + strand, within glgX at 83.554 kb on + strand, within glgX at 83.554 kb on + strand, within glgX at 83.554 kb on + strand at 83.562 kb on - strand, within glgX at 83.562 kb on - strand, within glgX at 83.586 kb on - strand, within glgX at 83.586 kb on - strand, within glgX at 83.586 kb on - strand, within glgX at 83.627 kb on + strand, within glgX at 83.627 kb on + strand, within glgX at 83.627 kb on + strand, within glgX at 83.627 kb on + strand, within glgX at 83.627 kb on + strand, within glgX at 83.635 kb on - strand, within glgX at 83.647 kb on + strand, within glgX at 83.647 kb on + strand, within glgX at 83.650 kb on - strand, within glgX at 83.690 kb on + strand, within glgX at 83.698 kb on - strand, within glgX at 83.741 kb on - strand, within glgX at 83.768 kb on - strand, within glgX at 83.783 kb on - strand at 83.803 kb on - strand, within glgX at 83.811 kb on - strand, within glgX
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 81,188 + Synpcc7942_0083 0.83 +2.0 81,194 + Synpcc7942_0083 0.86 +0.1 81,195 + Synpcc7942_0083 0.87 -1.3 81,196 + Synpcc7942_0083 0.87 +1.7 81,228 + -2.5 81,267 + +1.9 81,276 - +1.9 81,284 + +0.2 81,284 + +4.1 81,284 + +0.7 81,287 - -4.2 81,289 + Synpcc7942_0084 0.10 -0.8 81,289 + Synpcc7942_0084 0.10 -0.5 81,326 + Synpcc7942_0084 0.16 -0.6 81,334 - Synpcc7942_0084 0.17 +3.4 81,358 - Synpcc7942_0084 0.20 -0.9 81,368 + Synpcc7942_0084 0.22 -1.6 81,459 + Synpcc7942_0084 0.34 -0.6 81,459 + Synpcc7942_0084 0.34 +1.2 81,461 + Synpcc7942_0084 0.35 +0.5 81,467 - Synpcc7942_0084 0.36 +0.9 81,475 + Synpcc7942_0084 0.37 -1.2 81,483 - Synpcc7942_0084 0.38 +1.7 81,483 - Synpcc7942_0084 0.38 +2.6 81,488 - Synpcc7942_0084 0.39 +1.1 81,488 - Synpcc7942_0084 0.39 +5.1 81,488 - Synpcc7942_0084 0.39 -1.9 81,495 + Synpcc7942_0084 0.40 +3.1 81,495 + Synpcc7942_0084 0.40 -1.3 81,496 + Synpcc7942_0084 0.40 -0.6 81,503 + Synpcc7942_0084 0.41 +0.7 81,503 - Synpcc7942_0084 0.41 -2.6 81,504 - Synpcc7942_0084 0.41 +1.2 81,504 - Synpcc7942_0084 0.41 +0.4 81,535 - Synpcc7942_0084 0.45 +2.4 81,550 + Synpcc7942_0084 0.47 +0.7 81,558 + Synpcc7942_0084 0.49 +1.0 81,558 - Synpcc7942_0084 0.49 +0.2 81,566 - Synpcc7942_0084 0.50 +1.7 81,566 - Synpcc7942_0084 0.50 -2.6 81,584 + Synpcc7942_0084 0.52 +0.9 81,595 + Synpcc7942_0084 0.54 -1.8 81,619 + Synpcc7942_0084 0.57 +0.4 81,622 - Synpcc7942_0084 0.58 +1.5 81,624 + Synpcc7942_0084 0.58 -0.7 81,634 + Synpcc7942_0084 0.59 +1.5 81,644 - Synpcc7942_0084 0.61 -0.3 81,650 - Synpcc7942_0084 0.62 +1.7 81,691 + Synpcc7942_0084 0.68 +2.8 81,694 - Synpcc7942_0084 0.68 +0.9 81,754 + Synpcc7942_0084 0.76 +0.7 81,772 + Synpcc7942_0084 0.79 +0.8 81,772 - Synpcc7942_0084 0.79 +3.0 81,789 + -0.5 81,797 - Synpcc7942_0084 0.83 -2.4 81,808 - Synpcc7942_0084 0.84 -1.0 81,808 - Synpcc7942_0084 0.84 -0.1 81,854 + +0.7 81,854 + +1.2 81,854 + +1.7 81,854 + -1.7 81,857 - +4.3 81,862 - -0.1 81,864 - -1.1 81,918 + +1.8 81,925 + -2.1 81,925 + -0.2 81,925 + -2.1 81,925 + +0.8 81,925 + +3.7 81,925 + +1.1 81,925 + -0.7 81,933 - +1.7 81,933 - +1.4 81,945 + -0.8 81,984 + -0.9 81,984 + +0.9 81,996 + +2.1 81,998 + +0.9 82,004 - -0.8 82,004 - -1.4 82,004 - -0.1 82,004 - -2.2 82,004 - -0.7 82,004 - -2.3 82,017 + +0.3 82,029 + +0.2 82,040 + +2.2 82,086 + -1.5 82,123 + -1.7 82,123 + -1.5 82,147 + -1.1 82,147 + +1.1 82,147 + -2.1 82,147 + +0.1 82,148 + -1.2 82,148 + +0.7 82,148 + -1.6 82,155 - +0.1 82,161 - -1.4 82,170 + +0.8 82,170 + +0.6 82,178 - +1.7 82,183 + -3.8 82,183 + +0.5 82,183 + +0.9 82,185 - +0.0 82,191 - +2.2 82,195 + +1.3 82,195 + -0.8 82,203 - +2.4 82,227 - +0.5 82,293 - Synpcc7942_0085 0.17 -1.4 82,293 - Synpcc7942_0085 0.17 +1.5 82,296 + Synpcc7942_0085 0.17 -0.2 82,300 + Synpcc7942_0085 0.18 -1.1 82,373 - Synpcc7942_0085 0.28 -0.2 82,375 + Synpcc7942_0085 0.29 +3.2 82,380 + Synpcc7942_0085 0.29 +3.0 82,383 - Synpcc7942_0085 0.30 +0.8 82,429 + Synpcc7942_0085 0.36 -2.2 82,508 + Synpcc7942_0085 0.48 +3.4 82,516 - Synpcc7942_0085 0.49 +1.4 82,539 - Synpcc7942_0085 0.52 +0.3 82,541 + Synpcc7942_0085 0.53 +1.3 82,541 + Synpcc7942_0085 0.53 +1.3 82,541 + Synpcc7942_0085 0.53 +2.1 82,542 - Synpcc7942_0085 0.53 +2.1 82,546 + Synpcc7942_0085 0.53 -2.2 82,549 - Synpcc7942_0085 0.54 -0.6 82,552 - Synpcc7942_0085 0.54 +0.4 82,559 + Synpcc7942_0085 0.55 +2.8 82,559 + Synpcc7942_0085 0.55 +1.4 82,559 + Synpcc7942_0085 0.55 -1.5 82,570 + Synpcc7942_0085 0.57 +2.2 82,580 + Synpcc7942_0085 0.58 -0.5 82,679 - Synpcc7942_0085 0.73 +3.2 82,705 + Synpcc7942_0085 0.77 +1.7 82,705 + Synpcc7942_0085 0.77 +0.4 82,705 + Synpcc7942_0085 0.77 +1.4 82,713 - Synpcc7942_0085 0.78 +1.6 82,713 - Synpcc7942_0085 0.78 -1.1 82,717 + Synpcc7942_0085 0.78 +2.0 82,725 - Synpcc7942_0085 0.79 +3.3 82,725 - Synpcc7942_0085 0.79 -1.4 82,747 - Synpcc7942_0085 0.83 -1.2 82,889 - -0.2 82,914 + -2.6 82,922 - -2.4 82,982 + -4.1 82,983 + -3.9 82,991 - -4.3 83,041 + -2.3 83,043 + -0.1 83,055 + -3.1 83,055 + -3.5 83,096 + glgX Synpcc7942_0086 0.11 -3.1 83,107 + glgX Synpcc7942_0086 0.11 -1.9 83,128 + glgX Synpcc7942_0086 0.12 -0.9 83,177 - glgX Synpcc7942_0086 0.15 -3.6 83,198 - glgX Synpcc7942_0086 0.16 -3.3 83,215 + glgX Synpcc7942_0086 0.16 -1.5 83,250 - glgX Synpcc7942_0086 0.18 -2.1 83,334 + glgX Synpcc7942_0086 0.22 -3.3 83,342 - glgX Synpcc7942_0086 0.23 -2.6 83,342 - glgX Synpcc7942_0086 0.23 -2.0 83,391 - glgX Synpcc7942_0086 0.25 -0.9 83,391 - glgX Synpcc7942_0086 0.25 -1.0 83,393 + +0.1 83,405 + -1.6 83,408 - glgX Synpcc7942_0086 0.26 -2.4 83,426 + glgX Synpcc7942_0086 0.27 -1.7 83,520 + glgX Synpcc7942_0086 0.31 -1.6 83,543 + glgX Synpcc7942_0086 0.32 -2.4 83,554 + glgX Synpcc7942_0086 0.33 -1.0 83,554 + glgX Synpcc7942_0086 0.33 -0.5 83,554 + +0.1 83,562 - glgX Synpcc7942_0086 0.33 -2.7 83,562 - glgX Synpcc7942_0086 0.33 -0.4 83,586 - glgX Synpcc7942_0086 0.34 -2.0 83,586 - glgX Synpcc7942_0086 0.34 +0.6 83,586 - glgX Synpcc7942_0086 0.34 -1.2 83,627 + glgX Synpcc7942_0086 0.36 -3.2 83,627 + glgX Synpcc7942_0086 0.36 -2.7 83,627 + glgX Synpcc7942_0086 0.36 +0.5 83,627 + glgX Synpcc7942_0086 0.36 -3.4 83,627 + glgX Synpcc7942_0086 0.36 -2.4 83,635 - glgX Synpcc7942_0086 0.37 -3.8 83,647 + glgX Synpcc7942_0086 0.37 -2.6 83,647 + glgX Synpcc7942_0086 0.37 -3.4 83,650 - glgX Synpcc7942_0086 0.37 -2.2 83,690 + glgX Synpcc7942_0086 0.39 -2.3 83,698 - glgX Synpcc7942_0086 0.40 -2.0 83,741 - glgX Synpcc7942_0086 0.42 -1.1 83,768 - glgX Synpcc7942_0086 0.43 -2.0 83,783 - -0.7 83,803 - glgX Synpcc7942_0086 0.45 -2.7 83,811 - glgX Synpcc7942_0086 0.45 -2.0
Or see this region's nucleotide sequence