Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0050 and Synpcc7942_0051 overlap by 11 nucleotides Synpcc7942_0051 and Synpcc7942_0052 are separated by 4 nucleotides Synpcc7942_0052 and Synpcc7942_0053 are separated by 173 nucleotides
Synpcc7942_0050: Synpcc7942_0050 - hypothetical protein, at 50,070 to 52,616
_0050
Synpcc7942_0051: Synpcc7942_0051 - TPR repeat, at 52,606 to 54,507
_0051
Synpcc7942_0052: Synpcc7942_0052 - hypothetical protein, at 54,512 to 55,030
_0052
Synpcc7942_0053: Synpcc7942_0053 - hypothetical protein, at 55,204 to 56,352
_0053
Position (kb)
52
53
54
55 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 51.770 kb on + strand, within Synpcc7942_0050 at 51.811 kb on - strand, within Synpcc7942_0050 at 51.898 kb on + strand, within Synpcc7942_0050 at 51.899 kb on + strand, within Synpcc7942_0050 at 51.899 kb on + strand, within Synpcc7942_0050 at 51.918 kb on + strand, within Synpcc7942_0050 at 52.034 kb on + strand, within Synpcc7942_0050 at 52.042 kb on - strand at 52.098 kb on - strand, within Synpcc7942_0050 at 52.110 kb on + strand, within Synpcc7942_0050 at 52.118 kb on - strand, within Synpcc7942_0050 at 52.118 kb on - strand, within Synpcc7942_0050 at 52.118 kb on - strand, within Synpcc7942_0050 at 52.131 kb on + strand, within Synpcc7942_0050 at 52.280 kb on + strand, within Synpcc7942_0050 at 52.291 kb on + strand, within Synpcc7942_0050 at 52.353 kb on + strand, within Synpcc7942_0050 at 52.354 kb on + strand, within Synpcc7942_0050 at 52.362 kb on - strand at 52.381 kb on - strand at 52.428 kb on - strand at 52.430 kb on - strand at 52.685 kb on - strand at 52.691 kb on + strand at 52.700 kb on + strand at 52.724 kb on + strand at 52.724 kb on + strand at 52.724 kb on + strand at 52.724 kb on + strand at 52.732 kb on - strand at 52.732 kb on - strand at 52.732 kb on - strand at 52.788 kb on - strand at 52.850 kb on + strand, within Synpcc7942_0051 at 52.882 kb on + strand, within Synpcc7942_0051 at 52.898 kb on + strand, within Synpcc7942_0051 at 52.904 kb on - strand, within Synpcc7942_0051 at 52.928 kb on - strand, within Synpcc7942_0051 at 52.953 kb on + strand, within Synpcc7942_0051 at 52.953 kb on - strand, within Synpcc7942_0051 at 52.967 kb on + strand, within Synpcc7942_0051 at 52.967 kb on + strand, within Synpcc7942_0051 at 52.969 kb on + strand, within Synpcc7942_0051 at 52.975 kb on - strand, within Synpcc7942_0051 at 53.082 kb on - strand, within Synpcc7942_0051 at 53.154 kb on - strand, within Synpcc7942_0051 at 53.318 kb on + strand, within Synpcc7942_0051 at 53.318 kb on + strand, within Synpcc7942_0051 at 53.318 kb on + strand, within Synpcc7942_0051 at 53.325 kb on - strand, within Synpcc7942_0051 at 53.339 kb on - strand, within Synpcc7942_0051 at 53.412 kb on + strand, within Synpcc7942_0051 at 53.420 kb on - strand, within Synpcc7942_0051 at 53.691 kb on + strand, within Synpcc7942_0051 at 53.697 kb on - strand, within Synpcc7942_0051 at 53.816 kb on + strand, within Synpcc7942_0051 at 53.824 kb on - strand, within Synpcc7942_0051 at 53.892 kb on - strand, within Synpcc7942_0051 at 53.980 kb on + strand, within Synpcc7942_0051 at 54.026 kb on + strand, within Synpcc7942_0051 at 54.149 kb on - strand, within Synpcc7942_0051 at 54.316 kb on + strand, within Synpcc7942_0051 at 54.361 kb on - strand at 54.496 kb on - strand at 54.707 kb on - strand, within Synpcc7942_0052 at 54.755 kb on - strand, within Synpcc7942_0052 at 54.768 kb on - strand, within Synpcc7942_0052 at 54.768 kb on - strand, within Synpcc7942_0052 at 55.322 kb on + strand, within Synpcc7942_0053
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 51,770 + Synpcc7942_0050 0.67 -1.9 51,811 - Synpcc7942_0050 0.68 -0.4 51,898 + Synpcc7942_0050 0.72 -2.0 51,899 + Synpcc7942_0050 0.72 +0.6 51,899 + Synpcc7942_0050 0.72 +0.3 51,918 + Synpcc7942_0050 0.73 +0.3 52,034 + Synpcc7942_0050 0.77 +2.2 52,042 - +1.5 52,098 - Synpcc7942_0050 0.80 +1.0 52,110 + Synpcc7942_0050 0.80 +1.1 52,118 - Synpcc7942_0050 0.80 -1.1 52,118 - Synpcc7942_0050 0.80 -0.5 52,118 - Synpcc7942_0050 0.80 +0.1 52,131 + Synpcc7942_0050 0.81 +3.0 52,280 + Synpcc7942_0050 0.87 -0.1 52,291 + Synpcc7942_0050 0.87 -1.1 52,353 + Synpcc7942_0050 0.90 +0.2 52,354 + Synpcc7942_0050 0.90 -2.4 52,362 - +0.5 52,381 - -1.5 52,428 - +1.0 52,430 - +0.6 52,685 - -1.4 52,691 + -2.2 52,700 + -1.6 52,724 + -1.2 52,724 + -2.0 52,724 + -1.4 52,724 + +1.1 52,732 - -2.6 52,732 - -2.9 52,732 - -2.4 52,788 - -2.5 52,850 + Synpcc7942_0051 0.13 -3.5 52,882 + Synpcc7942_0051 0.15 -1.9 52,898 + Synpcc7942_0051 0.15 -1.6 52,904 - Synpcc7942_0051 0.16 -0.9 52,928 - Synpcc7942_0051 0.17 -2.1 52,953 + Synpcc7942_0051 0.18 -0.5 52,953 - Synpcc7942_0051 0.18 -1.0 52,967 + Synpcc7942_0051 0.19 -2.9 52,967 + Synpcc7942_0051 0.19 -2.2 52,969 + Synpcc7942_0051 0.19 -3.3 52,975 - Synpcc7942_0051 0.19 +0.1 53,082 - Synpcc7942_0051 0.25 -4.1 53,154 - Synpcc7942_0051 0.29 -2.5 53,318 + Synpcc7942_0051 0.37 -1.2 53,318 + Synpcc7942_0051 0.37 -3.3 53,318 + Synpcc7942_0051 0.37 -1.4 53,325 - Synpcc7942_0051 0.38 +0.5 53,339 - Synpcc7942_0051 0.39 -1.4 53,412 + Synpcc7942_0051 0.42 -3.5 53,420 - Synpcc7942_0051 0.43 -2.7 53,691 + Synpcc7942_0051 0.57 -4.0 53,697 - Synpcc7942_0051 0.57 -1.1 53,816 + Synpcc7942_0051 0.64 -1.4 53,824 - Synpcc7942_0051 0.64 -1.0 53,892 - Synpcc7942_0051 0.68 -0.5 53,980 + Synpcc7942_0051 0.72 -1.5 54,026 + Synpcc7942_0051 0.75 -1.7 54,149 - Synpcc7942_0051 0.81 -2.8 54,316 + Synpcc7942_0051 0.90 -0.9 54,361 - -2.0 54,496 - +1.9 54,707 - Synpcc7942_0052 0.38 -1.4 54,755 - Synpcc7942_0052 0.47 -0.3 54,768 - Synpcc7942_0052 0.49 -1.1 54,768 - Synpcc7942_0052 0.49 +1.7 55,322 + Synpcc7942_0053 0.10 +1.0
Or see this region's nucleotide sequence