Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gnd and folB are separated by 84 nucleotides folB and Synpcc7942_0041 are separated by 433 nucleotides Synpcc7942_0041 and rpmB are separated by 109 nucleotides rpmB and Synpcc7942_0043 are separated by 39 nucleotides Synpcc7942_0043 and Synpcc7942_0044 overlap by 2 nucleotides
Synpcc7942_0039: gnd - 6-phosphogluconate dehydrogenase, at 37,130 to 38,545
gnd
Synpcc7942_0040: folB - dihydroneopterin aldolase Dihydroneopterin aldolase, at 38,630 to 39,007
folB
Synpcc7942_0041: Synpcc7942_0041 - probable transport protein, at 39,441 to 40,745
_0041
Synpcc7942_0042: rpmB - 50S ribosomal protein L28, at 40,855 to 41,091
rpmB
Synpcc7942_0043: Synpcc7942_0043 - hypothetical protein, at 41,131 to 41,496
_0043
Synpcc7942_0044: Synpcc7942_0044 - hypothetical protein, at 41,495 to 43,477
_0044
Position (kb) 39
40
41 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 38.447 kb on - strand at 38.469 kb on - strand at 38.521 kb on - strand at 38.539 kb on - strand at 38.547 kb on - strand at 38.547 kb on - strand at 38.549 kb on + strand at 38.571 kb on + strand at 38.571 kb on + strand at 38.571 kb on + strand at 38.571 kb on + strand at 38.579 kb on - strand at 38.579 kb on - strand at 38.598 kb on + strand at 38.616 kb on - strand at 38.616 kb on - strand at 39.018 kb on + strand at 39.027 kb on - strand at 39.027 kb on - strand at 39.041 kb on - strand at 39.327 kb on + strand at 39.334 kb on + strand at 39.334 kb on + strand at 39.342 kb on - strand at 39.342 kb on - strand at 39.342 kb on - strand at 39.378 kb on + strand at 39.378 kb on + strand at 39.386 kb on - strand at 39.396 kb on - strand at 39.398 kb on - strand at 39.415 kb on + strand at 39.438 kb on + strand at 39.454 kb on - strand at 39.464 kb on - strand at 39.464 kb on - strand at 39.499 kb on + strand at 39.499 kb on + strand at 39.499 kb on + strand at 39.507 kb on - strand at 39.507 kb on - strand at 39.507 kb on - strand at 39.533 kb on + strand at 39.536 kb on - strand at 39.543 kb on + strand at 39.590 kb on + strand, within Synpcc7942_0041 at 39.652 kb on + strand, within Synpcc7942_0041 at 39.659 kb on - strand, within Synpcc7942_0041 at 39.659 kb on - strand, within Synpcc7942_0041 at 39.669 kb on + strand, within Synpcc7942_0041 at 39.671 kb on + strand, within Synpcc7942_0041 at 39.671 kb on - strand, within Synpcc7942_0041 at 39.679 kb on + strand, within Synpcc7942_0041 at 39.696 kb on + strand, within Synpcc7942_0041 at 39.696 kb on + strand, within Synpcc7942_0041 at 39.696 kb on + strand, within Synpcc7942_0041 at 39.696 kb on + strand, within Synpcc7942_0041 at 39.696 kb on + strand, within Synpcc7942_0041 at 39.696 kb on + strand, within Synpcc7942_0041 at 39.696 kb on + strand, within Synpcc7942_0041 at 39.696 kb on + strand, within Synpcc7942_0041 at 39.704 kb on - strand, within Synpcc7942_0041 at 39.704 kb on - strand, within Synpcc7942_0041 at 39.744 kb on - strand, within Synpcc7942_0041 at 39.749 kb on + strand, within Synpcc7942_0041 at 39.768 kb on + strand, within Synpcc7942_0041 at 39.768 kb on + strand, within Synpcc7942_0041 at 39.768 kb on + strand, within Synpcc7942_0041 at 39.776 kb on - strand, within Synpcc7942_0041 at 39.776 kb on - strand, within Synpcc7942_0041 at 39.787 kb on - strand, within Synpcc7942_0041 at 39.789 kb on + strand, within Synpcc7942_0041 at 39.830 kb on + strand at 39.830 kb on + strand, within Synpcc7942_0041 at 39.838 kb on - strand, within Synpcc7942_0041 at 39.846 kb on + strand, within Synpcc7942_0041 at 39.849 kb on - strand, within Synpcc7942_0041 at 39.849 kb on - strand, within Synpcc7942_0041 at 39.850 kb on + strand, within Synpcc7942_0041 at 39.854 kb on - strand, within Synpcc7942_0041 at 39.858 kb on + strand, within Synpcc7942_0041 at 39.860 kb on - strand, within Synpcc7942_0041 at 39.860 kb on - strand, within Synpcc7942_0041 at 39.860 kb on - strand, within Synpcc7942_0041 at 39.860 kb on - strand, within Synpcc7942_0041 at 39.860 kb on - strand, within Synpcc7942_0041 at 39.878 kb on + strand, within Synpcc7942_0041 at 39.919 kb on + strand, within Synpcc7942_0041 at 39.926 kb on - strand, within Synpcc7942_0041 at 39.945 kb on - strand, within Synpcc7942_0041 at 39.949 kb on + strand, within Synpcc7942_0041 at 39.959 kb on + strand, within Synpcc7942_0041 at 39.971 kb on + strand, within Synpcc7942_0041 at 39.979 kb on - strand, within Synpcc7942_0041 at 40.006 kb on - strand, within Synpcc7942_0041 at 40.014 kb on + strand, within Synpcc7942_0041 at 40.059 kb on - strand, within Synpcc7942_0041 at 40.066 kb on + strand, within Synpcc7942_0041 at 40.105 kb on - strand, within Synpcc7942_0041 at 40.129 kb on - strand, within Synpcc7942_0041 at 40.132 kb on + strand, within Synpcc7942_0041 at 40.137 kb on + strand, within Synpcc7942_0041 at 40.145 kb on - strand, within Synpcc7942_0041 at 40.157 kb on + strand at 40.158 kb on + strand, within Synpcc7942_0041 at 40.177 kb on + strand, within Synpcc7942_0041 at 40.185 kb on - strand, within Synpcc7942_0041 at 40.185 kb on - strand, within Synpcc7942_0041 at 40.194 kb on - strand, within Synpcc7942_0041 at 40.249 kb on - strand, within Synpcc7942_0041 at 40.254 kb on + strand, within Synpcc7942_0041 at 40.276 kb on + strand at 40.281 kb on + strand, within Synpcc7942_0041 at 40.281 kb on + strand, within Synpcc7942_0041 at 40.286 kb on + strand, within Synpcc7942_0041 at 40.289 kb on - strand, within Synpcc7942_0041 at 40.289 kb on - strand, within Synpcc7942_0041 at 40.313 kb on + strand, within Synpcc7942_0041 at 40.321 kb on - strand, within Synpcc7942_0041 at 40.323 kb on + strand, within Synpcc7942_0041 at 40.330 kb on + strand, within Synpcc7942_0041 at 40.336 kb on + strand, within Synpcc7942_0041 at 40.336 kb on + strand, within Synpcc7942_0041 at 40.344 kb on - strand, within Synpcc7942_0041 at 40.344 kb on - strand, within Synpcc7942_0041 at 40.359 kb on + strand, within Synpcc7942_0041 at 40.368 kb on + strand, within Synpcc7942_0041 at 40.397 kb on - strand, within Synpcc7942_0041 at 40.406 kb on - strand, within Synpcc7942_0041 at 40.415 kb on + strand, within Synpcc7942_0041 at 40.423 kb on - strand, within Synpcc7942_0041 at 40.447 kb on + strand, within Synpcc7942_0041 at 40.490 kb on + strand, within Synpcc7942_0041 at 40.494 kb on - strand, within Synpcc7942_0041 at 40.516 kb on + strand, within Synpcc7942_0041 at 40.538 kb on + strand, within Synpcc7942_0041 at 40.541 kb on - strand, within Synpcc7942_0041 at 40.541 kb on - strand, within Synpcc7942_0041 at 40.546 kb on - strand, within Synpcc7942_0041 at 40.547 kb on + strand, within Synpcc7942_0041 at 40.550 kb on + strand, within Synpcc7942_0041 at 40.552 kb on + strand, within Synpcc7942_0041 at 40.558 kb on - strand, within Synpcc7942_0041 at 40.573 kb on - strand, within Synpcc7942_0041 at 40.583 kb on + strand, within Synpcc7942_0041 at 40.596 kb on - strand, within Synpcc7942_0041 at 40.596 kb on - strand, within Synpcc7942_0041 at 40.632 kb on - strand at 40.651 kb on + strand at 40.676 kb on + strand at 40.676 kb on + strand at 40.676 kb on + strand at 40.676 kb on + strand at 40.684 kb on - strand at 40.684 kb on - strand at 40.719 kb on + strand at 40.719 kb on - strand at 40.721 kb on + strand at 40.721 kb on + strand at 40.721 kb on + strand at 40.721 kb on + strand at 40.727 kb on - strand at 40.729 kb on - strand at 40.729 kb on - strand at 40.729 kb on - strand at 40.729 kb on - strand at 40.745 kb on + strand at 40.754 kb on - strand at 40.805 kb on + strand at 40.810 kb on + strand at 40.815 kb on + strand at 40.818 kb on - strand at 40.823 kb on - strand at 40.843 kb on - strand at 40.844 kb on - strand at 40.853 kb on + strand at 40.861 kb on - strand at 40.904 kb on + strand, within rpmB at 40.917 kb on - strand, within rpmB at 41.041 kb on + strand, within rpmB at 41.042 kb on + strand, within rpmB at 41.050 kb on - strand, within rpmB at 41.130 kb on + strand at 41.138 kb on + strand at 41.138 kb on - strand at 41.176 kb on + strand, within Synpcc7942_0043 at 41.192 kb on - strand, within Synpcc7942_0043 at 41.194 kb on + strand, within Synpcc7942_0043 at 41.212 kb on + strand, within Synpcc7942_0043 at 41.212 kb on + strand at 41.220 kb on - strand, within Synpcc7942_0043 at 41.256 kb on - strand, within Synpcc7942_0043 at 41.264 kb on - strand, within Synpcc7942_0043 at 41.277 kb on - strand, within Synpcc7942_0043 at 41.297 kb on - strand, within Synpcc7942_0043 at 41.321 kb on - strand, within Synpcc7942_0043 at 41.339 kb on + strand, within Synpcc7942_0043 at 41.339 kb on + strand, within Synpcc7942_0043 at 41.347 kb on - strand, within Synpcc7942_0043 at 41.372 kb on + strand, within Synpcc7942_0043 at 41.391 kb on - strand, within Synpcc7942_0043 at 41.415 kb on - strand, within Synpcc7942_0043 at 41.420 kb on - strand, within Synpcc7942_0043 at 41.440 kb on + strand, within Synpcc7942_0043 at 41.492 kb on + strand at 41.492 kb on + strand at 41.492 kb on + strand at 41.492 kb on + strand at 41.492 kb on + strand at 41.495 kb on - strand at 41.499 kb on + strand at 41.500 kb on - strand at 41.500 kb on - strand at 41.500 kb on - strand at 41.543 kb on + strand at 41.544 kb on - strand at 41.558 kb on - strand at 41.558 kb on - strand at 41.579 kb on - strand at 41.579 kb on - strand at 41.581 kb on + strand at 41.581 kb on + strand at 41.581 kb on - strand at 41.585 kb on + strand at 41.585 kb on + strand at 41.585 kb on + strand at 41.585 kb on + strand at 41.585 kb on + strand at 41.589 kb on - strand at 41.593 kb on - strand at 41.593 kb on - strand at 41.593 kb on - strand at 41.593 kb on - strand at 41.593 kb on - strand at 41.643 kb on + strand at 41.651 kb on - strand at 41.659 kb on - strand at 41.675 kb on - strand at 41.706 kb on - strand, within Synpcc7942_0044 at 41.710 kb on - strand, within Synpcc7942_0044 at 41.712 kb on + strand, within Synpcc7942_0044 at 41.717 kb on - strand, within Synpcc7942_0044 at 41.719 kb on + strand, within Synpcc7942_0044 at 41.719 kb on + strand, within Synpcc7942_0044 at 41.732 kb on - strand, within Synpcc7942_0044 at 41.732 kb on - strand, within Synpcc7942_0044 at 41.734 kb on + strand, within Synpcc7942_0044 at 41.734 kb on + strand, within Synpcc7942_0044 at 41.734 kb on + strand, within Synpcc7942_0044 at 41.742 kb on - strand, within Synpcc7942_0044 at 41.745 kb on + strand, within Synpcc7942_0044
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 38,447 - -1.9 38,469 - +2.3 38,521 - -0.6 38,539 - -1.2 38,547 - +1.1 38,547 - -0.5 38,549 + +0.3 38,571 + +1.0 38,571 + -1.0 38,571 + +0.1 38,571 + -1.6 38,579 - +0.5 38,579 - +0.1 38,598 + +0.8 38,616 - +1.3 38,616 - -2.1 39,018 + -1.1 39,027 - +4.6 39,027 - -0.5 39,041 - +2.5 39,327 + -1.5 39,334 + -2.4 39,334 + -2.6 39,342 - -0.0 39,342 - -2.5 39,342 - -4.5 39,378 + -4.5 39,378 + -2.2 39,386 - -0.1 39,396 - -3.2 39,398 - +1.4 39,415 + +4.2 39,438 + -1.6 39,454 - -0.2 39,464 - -0.7 39,464 - -0.2 39,499 + -2.3 39,499 + -1.8 39,499 + +0.5 39,507 - -1.7 39,507 - -3.7 39,507 - -1.5 39,533 + -1.4 39,536 - -1.0 39,543 + -3.7 39,590 + Synpcc7942_0041 0.11 -2.8 39,652 + Synpcc7942_0041 0.16 -1.9 39,659 - Synpcc7942_0041 0.17 -3.9 39,659 - Synpcc7942_0041 0.17 -0.1 39,669 + Synpcc7942_0041 0.17 -3.5 39,671 + Synpcc7942_0041 0.18 -1.7 39,671 - Synpcc7942_0041 0.18 -2.6 39,679 + Synpcc7942_0041 0.18 -3.0 39,696 + Synpcc7942_0041 0.20 -1.7 39,696 + Synpcc7942_0041 0.20 -0.8 39,696 + Synpcc7942_0041 0.20 -2.0 39,696 + Synpcc7942_0041 0.20 -1.4 39,696 + Synpcc7942_0041 0.20 -0.2 39,696 + Synpcc7942_0041 0.20 -1.0 39,696 + Synpcc7942_0041 0.20 -1.9 39,696 + Synpcc7942_0041 0.20 -2.8 39,704 - Synpcc7942_0041 0.20 -1.7 39,704 - Synpcc7942_0041 0.20 -2.2 39,744 - Synpcc7942_0041 0.23 -1.6 39,749 + Synpcc7942_0041 0.24 -4.1 39,768 + Synpcc7942_0041 0.25 -1.6 39,768 + Synpcc7942_0041 0.25 -3.5 39,768 + Synpcc7942_0041 0.25 -2.2 39,776 - Synpcc7942_0041 0.26 -2.6 39,776 - Synpcc7942_0041 0.26 -2.0 39,787 - Synpcc7942_0041 0.27 -2.4 39,789 + Synpcc7942_0041 0.27 -2.4 39,830 + +0.8 39,830 + Synpcc7942_0041 0.30 -0.7 39,838 - Synpcc7942_0041 0.30 -2.8 39,846 + Synpcc7942_0041 0.31 -1.2 39,849 - Synpcc7942_0041 0.31 -3.4 39,849 - Synpcc7942_0041 0.31 -2.0 39,850 + Synpcc7942_0041 0.31 -2.6 39,854 - Synpcc7942_0041 0.32 -2.4 39,858 + Synpcc7942_0041 0.32 -4.1 39,860 - Synpcc7942_0041 0.32 -1.7 39,860 - Synpcc7942_0041 0.32 -1.2 39,860 - Synpcc7942_0041 0.32 -3.0 39,860 - Synpcc7942_0041 0.32 +1.5 39,860 - Synpcc7942_0041 0.32 -3.8 39,878 + Synpcc7942_0041 0.33 -0.8 39,919 + Synpcc7942_0041 0.37 -0.5 39,926 - Synpcc7942_0041 0.37 -2.2 39,945 - Synpcc7942_0041 0.39 -4.0 39,949 + Synpcc7942_0041 0.39 -2.7 39,959 + Synpcc7942_0041 0.40 -2.9 39,971 + Synpcc7942_0041 0.41 -3.2 39,979 - Synpcc7942_0041 0.41 -2.3 40,006 - Synpcc7942_0041 0.43 -2.8 40,014 + Synpcc7942_0041 0.44 -2.5 40,059 - Synpcc7942_0041 0.47 -2.9 40,066 + Synpcc7942_0041 0.48 -2.8 40,105 - Synpcc7942_0041 0.51 -1.2 40,129 - Synpcc7942_0041 0.53 -0.5 40,132 + Synpcc7942_0041 0.53 -2.0 40,137 + Synpcc7942_0041 0.53 -1.2 40,145 - Synpcc7942_0041 0.54 -1.7 40,157 + -1.6 40,158 + Synpcc7942_0041 0.55 -2.3 40,177 + Synpcc7942_0041 0.56 -1.6 40,185 - Synpcc7942_0041 0.57 -1.9 40,185 - Synpcc7942_0041 0.57 -3.6 40,194 - Synpcc7942_0041 0.58 -0.5 40,249 - Synpcc7942_0041 0.62 -1.7 40,254 + Synpcc7942_0041 0.62 -0.1 40,276 + -0.5 40,281 + Synpcc7942_0041 0.64 -1.1 40,281 + Synpcc7942_0041 0.64 -2.4 40,286 + Synpcc7942_0041 0.65 -1.2 40,289 - Synpcc7942_0041 0.65 -3.3 40,289 - Synpcc7942_0041 0.65 -0.5 40,313 + Synpcc7942_0041 0.67 -2.3 40,321 - Synpcc7942_0041 0.67 -3.0 40,323 + Synpcc7942_0041 0.68 -1.7 40,330 + Synpcc7942_0041 0.68 -4.3 40,336 + Synpcc7942_0041 0.69 -1.8 40,336 + Synpcc7942_0041 0.69 -3.2 40,344 - Synpcc7942_0041 0.69 -0.9 40,344 - Synpcc7942_0041 0.69 -1.7 40,359 + Synpcc7942_0041 0.70 -2.6 40,368 + Synpcc7942_0041 0.71 -3.0 40,397 - Synpcc7942_0041 0.73 +0.5 40,406 - Synpcc7942_0041 0.74 -0.2 40,415 + Synpcc7942_0041 0.75 -4.8 40,423 - Synpcc7942_0041 0.75 -4.6 40,447 + Synpcc7942_0041 0.77 -3.0 40,490 + Synpcc7942_0041 0.80 -3.2 40,494 - Synpcc7942_0041 0.81 -2.7 40,516 + Synpcc7942_0041 0.82 -0.2 40,538 + Synpcc7942_0041 0.84 -1.2 40,541 - Synpcc7942_0041 0.84 -1.8 40,541 - Synpcc7942_0041 0.84 -1.7 40,546 - Synpcc7942_0041 0.85 -2.6 40,547 + Synpcc7942_0041 0.85 -0.9 40,550 + Synpcc7942_0041 0.85 -3.0 40,552 + Synpcc7942_0041 0.85 -2.4 40,558 - Synpcc7942_0041 0.86 -3.4 40,573 - Synpcc7942_0041 0.87 -3.7 40,583 + Synpcc7942_0041 0.88 -4.2 40,596 - Synpcc7942_0041 0.89 -2.9 40,596 - Synpcc7942_0041 0.89 -1.6 40,632 - -2.1 40,651 + -4.0 40,676 + -2.4 40,676 + -2.4 40,676 + -5.5 40,676 + -1.5 40,684 - -1.8 40,684 - -2.5 40,719 + -4.7 40,719 - -4.0 40,721 + -3.8 40,721 + -0.8 40,721 + -0.5 40,721 + -1.1 40,727 - -2.7 40,729 - -1.7 40,729 - -2.4 40,729 - -1.4 40,729 - -2.3 40,745 + +1.5 40,754 - +2.5 40,805 + +0.5 40,810 + +1.3 40,815 + +2.0 40,818 - +0.0 40,823 - +0.7 40,843 - -0.1 40,844 - -1.3 40,853 + +1.6 40,861 - -0.5 40,904 + rpmB Synpcc7942_0042 0.21 +2.4 40,917 - rpmB Synpcc7942_0042 0.26 -1.8 41,041 + rpmB Synpcc7942_0042 0.78 -0.3 41,042 + rpmB Synpcc7942_0042 0.79 +0.8 41,050 - rpmB Synpcc7942_0042 0.82 -1.4 41,130 + -0.1 41,138 + +2.3 41,138 - +0.4 41,176 + Synpcc7942_0043 0.12 -0.2 41,192 - Synpcc7942_0043 0.17 +0.1 41,194 + Synpcc7942_0043 0.17 +0.3 41,212 + Synpcc7942_0043 0.22 -3.2 41,212 + -0.2 41,220 - Synpcc7942_0043 0.24 +2.0 41,256 - Synpcc7942_0043 0.34 +0.3 41,264 - Synpcc7942_0043 0.36 +1.5 41,277 - Synpcc7942_0043 0.40 +0.3 41,297 - Synpcc7942_0043 0.45 -1.4 41,321 - Synpcc7942_0043 0.52 -2.3 41,339 + Synpcc7942_0043 0.57 -1.4 41,339 + Synpcc7942_0043 0.57 +0.3 41,347 - Synpcc7942_0043 0.59 +1.8 41,372 + Synpcc7942_0043 0.66 -1.7 41,391 - Synpcc7942_0043 0.71 -2.7 41,415 - Synpcc7942_0043 0.78 +0.0 41,420 - Synpcc7942_0043 0.79 +2.0 41,440 + Synpcc7942_0043 0.84 -2.8 41,492 + -1.2 41,492 + -1.4 41,492 + -2.1 41,492 + +1.1 41,492 + -1.2 41,495 - -3.1 41,499 + +0.4 41,500 - -1.2 41,500 - -2.6 41,500 - -1.4 41,543 + -2.7 41,544 - +1.2 41,558 - +2.2 41,558 - -0.9 41,579 - +0.5 41,579 - +0.8 41,581 + +0.8 41,581 + +2.4 41,581 - -1.1 41,585 + -0.5 41,585 + -0.5 41,585 + -0.9 41,585 + +1.2 41,585 + -0.9 41,589 - +2.4 41,593 - -0.8 41,593 - -0.7 41,593 - +0.5 41,593 - -0.5 41,593 - -2.9 41,643 + +3.4 41,651 - -1.9 41,659 - -2.3 41,675 - +2.1 41,706 - Synpcc7942_0044 0.11 +0.4 41,710 - Synpcc7942_0044 0.11 -0.9 41,712 + Synpcc7942_0044 0.11 +2.9 41,717 - Synpcc7942_0044 0.11 -0.2 41,719 + Synpcc7942_0044 0.11 +0.5 41,719 + Synpcc7942_0044 0.11 +2.5 41,732 - Synpcc7942_0044 0.12 +4.2 41,732 - Synpcc7942_0044 0.12 +1.0 41,734 + Synpcc7942_0044 0.12 -0.5 41,734 + Synpcc7942_0044 0.12 -2.1 41,734 + Synpcc7942_0044 0.12 +2.9 41,742 - Synpcc7942_0044 0.12 +2.1 41,745 + Synpcc7942_0044 0.13 +0.9
Or see this region's nucleotide sequence