Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_889 and Pf6N2E2_890 are separated by 101 nucleotides Pf6N2E2_890 and Pf6N2E2_891 are separated by 93 nucleotides Pf6N2E2_891 and Pf6N2E2_892 are separated by 72 nucleotides Pf6N2E2_892 and Pf6N2E2_893 overlap by 4 nucleotides
Pf6N2E2_889 - Hydrolases of the alpha/beta superfamily, at 1,043,870 to 1,044,631
_889
Pf6N2E2_890 - Nitrilotriacetate monooxygenase component B (EC 1.14.13.-), at 1,044,733 to 1,045,353
_890
Pf6N2E2_891 - 4-oxalocrotonate tautomerase, at 1,045,447 to 1,045,623
_891
Pf6N2E2_892 - hypothetical protein, at 1,045,696 to 1,045,920
_892
Pf6N2E2_893 - hypothetical protein, at 1,045,917 to 1,046,153
_893
Position (kb)
1045
1046 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1044.513 kb on + strand, within Pf6N2E2_889 at 1044.514 kb on - strand, within Pf6N2E2_889 at 1044.567 kb on + strand at 1044.568 kb on - strand at 1044.568 kb on - strand at 1044.568 kb on - strand at 1044.620 kb on - strand at 1044.646 kb on + strand at 1044.646 kb on + strand at 1044.646 kb on + strand at 1044.646 kb on + strand at 1044.646 kb on + strand at 1044.646 kb on + strand at 1044.646 kb on + strand at 1044.647 kb on - strand at 1044.647 kb on - strand at 1044.676 kb on + strand at 1044.676 kb on + strand at 1044.836 kb on + strand, within Pf6N2E2_890 at 1044.837 kb on - strand, within Pf6N2E2_890 at 1044.880 kb on + strand, within Pf6N2E2_890 at 1044.881 kb on - strand, within Pf6N2E2_890 at 1044.881 kb on - strand, within Pf6N2E2_890 at 1044.881 kb on - strand, within Pf6N2E2_890 at 1044.881 kb on - strand, within Pf6N2E2_890 at 1045.114 kb on + strand, within Pf6N2E2_890 at 1045.201 kb on + strand, within Pf6N2E2_890 at 1045.201 kb on + strand, within Pf6N2E2_890 at 1045.230 kb on + strand, within Pf6N2E2_890 at 1045.235 kb on - strand, within Pf6N2E2_890 at 1045.235 kb on - strand, within Pf6N2E2_890 at 1045.353 kb on + strand at 1045.358 kb on + strand at 1045.359 kb on - strand at 1045.359 kb on - strand at 1045.359 kb on - strand at 1045.422 kb on - strand at 1045.448 kb on + strand at 1045.457 kb on - strand at 1045.532 kb on + strand, within Pf6N2E2_891 at 1045.532 kb on + strand, within Pf6N2E2_891 at 1045.533 kb on - strand, within Pf6N2E2_891 at 1045.533 kb on - strand, within Pf6N2E2_891 at 1045.533 kb on - strand, within Pf6N2E2_891 at 1045.533 kb on - strand, within Pf6N2E2_891 at 1045.533 kb on - strand, within Pf6N2E2_891 at 1045.889 kb on + strand, within Pf6N2E2_892 at 1045.892 kb on + strand, within Pf6N2E2_892 at 1045.918 kb on + strand at 1045.918 kb on + strand at 1045.919 kb on - strand at 1045.919 kb on - strand at 1045.919 kb on - strand at 1045.919 kb on - strand at 1046.016 kb on - strand, within Pf6N2E2_893 at 1046.101 kb on + strand, within Pf6N2E2_893 at 1046.102 kb on - strand, within Pf6N2E2_893 at 1046.102 kb on - strand, within Pf6N2E2_893 at 1046.150 kb on - strand at 1046.150 kb on - strand at 1046.321 kb on + strand at 1046.339 kb on - strand at 1046.339 kb on - strand at 1046.339 kb on - strand at 1046.382 kb on - strand at 1046.557 kb on + strand at 1046.557 kb on + strand at 1046.558 kb on - strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.579 kb on - strand at 1046.579 kb on - strand at 1046.579 kb on - strand at 1046.579 kb on - strand at 1046.579 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 1,044,513 + Pf6N2E2_889 0.84 +0.5 1,044,514 - Pf6N2E2_889 0.85 -0.4 1,044,567 + +0.1 1,044,568 - +0.2 1,044,568 - -1.1 1,044,568 - +0.3 1,044,620 - -0.4 1,044,646 + +0.6 1,044,646 + -0.6 1,044,646 + -0.2 1,044,646 + -0.4 1,044,646 + +0.4 1,044,646 + +1.6 1,044,646 + -0.4 1,044,647 - +2.2 1,044,647 - +0.7 1,044,676 + +0.4 1,044,676 + +0.4 1,044,836 + Pf6N2E2_890 0.17 +1.1 1,044,837 - Pf6N2E2_890 0.17 -0.8 1,044,880 + Pf6N2E2_890 0.24 -0.2 1,044,881 - Pf6N2E2_890 0.24 +0.5 1,044,881 - Pf6N2E2_890 0.24 -1.4 1,044,881 - Pf6N2E2_890 0.24 -0.8 1,044,881 - Pf6N2E2_890 0.24 -0.9 1,045,114 + Pf6N2E2_890 0.61 +0.9 1,045,201 + Pf6N2E2_890 0.75 -1.9 1,045,201 + Pf6N2E2_890 0.75 -0.5 1,045,230 + Pf6N2E2_890 0.80 +0.4 1,045,235 - Pf6N2E2_890 0.81 +0.1 1,045,235 - Pf6N2E2_890 0.81 +0.9 1,045,353 + -0.4 1,045,358 + -0.8 1,045,359 - +0.4 1,045,359 - +0.8 1,045,359 - -0.6 1,045,422 - -1.1 1,045,448 + -0.2 1,045,457 - +0.7 1,045,532 + Pf6N2E2_891 0.48 -1.1 1,045,532 + Pf6N2E2_891 0.48 -2.5 1,045,533 - Pf6N2E2_891 0.49 -0.9 1,045,533 - Pf6N2E2_891 0.49 +0.6 1,045,533 - Pf6N2E2_891 0.49 -1.6 1,045,533 - Pf6N2E2_891 0.49 +1.0 1,045,533 - Pf6N2E2_891 0.49 -0.5 1,045,889 + Pf6N2E2_892 0.86 +1.6 1,045,892 + Pf6N2E2_892 0.87 -0.4 1,045,918 + +0.1 1,045,918 + -0.8 1,045,919 - -0.3 1,045,919 - -0.0 1,045,919 - -0.8 1,045,919 - -0.0 1,046,016 - Pf6N2E2_893 0.42 +0.1 1,046,101 + Pf6N2E2_893 0.78 +0.2 1,046,102 - Pf6N2E2_893 0.78 -0.7 1,046,102 - Pf6N2E2_893 0.78 +0.7 1,046,150 - +0.5 1,046,150 - -0.5 1,046,321 + +0.4 1,046,339 - -0.4 1,046,339 - -0.1 1,046,339 - +0.2 1,046,382 - -0.8 1,046,557 + -1.2 1,046,557 + +0.7 1,046,558 - -0.0 1,046,578 + +1.2 1,046,578 + +0.0 1,046,578 + -1.9 1,046,578 + -1.9 1,046,578 + +0.6 1,046,578 + -0.3 1,046,578 + -1.5 1,046,578 + +1.3 1,046,578 + -0.7 1,046,578 + +0.9 1,046,578 + +1.4 1,046,578 + -1.0 1,046,579 - -0.5 1,046,579 - +0.3 1,046,579 - +0.4 1,046,579 - +0.1 1,046,579 - +0.5
Or see this region's nucleotide sequence