Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_874 and Pf6N2E2_875 are separated by 60 nucleotides Pf6N2E2_875 and Pf6N2E2_876 overlap by 4 nucleotides Pf6N2E2_876 and Pf6N2E2_877 are separated by 19 nucleotides Pf6N2E2_877 and Pf6N2E2_878 are separated by 76 nucleotides
Pf6N2E2_874 - Predicted cobalt transporter CbtC, at 1,024,468 to 1,025,145
_874
Pf6N2E2_875 - putative metal chaperone, involved in Fe-nitrile hydratase activation, GTPase of COG0523 family, at 1,025,206 to 1,025,646
_875
Pf6N2E2_876 - Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84), at 1,025,643 to 1,026,296
_876
Pf6N2E2_877 - Cobalt-containing nitrile hydratase subunit alpha (EC 4.2.1.84), at 1,026,316 to 1,026,948
_877
Pf6N2E2_878 - Amidase clustered with urea ABC transporter and nitrile hydratase functions, at 1,027,025 to 1,028,539
_878
Position (kb)
1025
1026
1027 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1024.643 kb on + strand, within Pf6N2E2_874 at 1024.644 kb on - strand, within Pf6N2E2_874 at 1024.713 kb on + strand, within Pf6N2E2_874 at 1024.714 kb on - strand, within Pf6N2E2_874 at 1024.714 kb on - strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.731 kb on - strand, within Pf6N2E2_874 at 1024.731 kb on - strand, within Pf6N2E2_874 at 1024.814 kb on + strand, within Pf6N2E2_874 at 1024.815 kb on - strand, within Pf6N2E2_874 at 1024.963 kb on + strand, within Pf6N2E2_874 at 1024.984 kb on + strand, within Pf6N2E2_874 at 1024.984 kb on + strand, within Pf6N2E2_874 at 1024.985 kb on - strand, within Pf6N2E2_874 at 1024.985 kb on - strand, within Pf6N2E2_874 at 1025.069 kb on + strand, within Pf6N2E2_874 at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.097 kb on - strand at 1025.097 kb on - strand at 1025.130 kb on + strand at 1025.322 kb on + strand, within Pf6N2E2_875 at 1025.330 kb on + strand, within Pf6N2E2_875 at 1025.330 kb on + strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.365 kb on - strand, within Pf6N2E2_875 at 1025.365 kb on - strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.491 kb on + strand, within Pf6N2E2_875 at 1025.492 kb on - strand, within Pf6N2E2_875 at 1025.621 kb on + strand at 1025.622 kb on - strand at 1025.622 kb on - strand at 1025.622 kb on - strand at 1025.622 kb on - strand at 1025.647 kb on - strand at 1025.660 kb on - strand at 1025.660 kb on - strand at 1025.660 kb on - strand at 1025.670 kb on + strand at 1025.670 kb on + strand at 1025.737 kb on + strand, within Pf6N2E2_876 at 1025.761 kb on - strand, within Pf6N2E2_876 at 1025.976 kb on + strand, within Pf6N2E2_876 at 1026.092 kb on - strand, within Pf6N2E2_876 at 1026.095 kb on - strand, within Pf6N2E2_876 at 1026.095 kb on - strand, within Pf6N2E2_876 at 1026.109 kb on + strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.269 kb on + strand at 1026.269 kb on + strand at 1026.270 kb on - strand at 1026.277 kb on - strand at 1026.286 kb on - strand at 1026.434 kb on - strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.473 kb on - strand, within Pf6N2E2_877 at 1026.493 kb on - strand, within Pf6N2E2_877 at 1026.493 kb on - strand, within Pf6N2E2_877 at 1026.563 kb on + strand, within Pf6N2E2_877 at 1026.563 kb on + strand, within Pf6N2E2_877 at 1026.737 kb on + strand, within Pf6N2E2_877 at 1026.738 kb on - strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.940 kb on - strand at 1026.953 kb on + strand at 1026.953 kb on + strand at 1026.954 kb on - strand at 1026.971 kb on + strand at 1026.987 kb on + strand at 1026.987 kb on + strand at 1026.988 kb on - strand at 1026.988 kb on - strand at 1026.988 kb on - strand at 1027.026 kb on + strand at 1027.027 kb on - strand at 1027.092 kb on + strand at 1027.171 kb on + strand at 1027.296 kb on + strand, within Pf6N2E2_878 at 1027.296 kb on + strand, within Pf6N2E2_878 at 1027.296 kb on + strand, within Pf6N2E2_878
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 1,024,643 + Pf6N2E2_874 0.26 +0.3 1,024,644 - Pf6N2E2_874 0.26 +0.0 1,024,713 + Pf6N2E2_874 0.36 -0.5 1,024,714 - Pf6N2E2_874 0.36 -0.7 1,024,714 - Pf6N2E2_874 0.36 +0.3 1,024,715 + Pf6N2E2_874 0.36 +0.3 1,024,715 + Pf6N2E2_874 0.36 +0.3 1,024,715 + Pf6N2E2_874 0.36 +0.3 1,024,716 - Pf6N2E2_874 0.37 -0.3 1,024,716 - Pf6N2E2_874 0.37 -1.3 1,024,716 - Pf6N2E2_874 0.37 +1.6 1,024,730 + Pf6N2E2_874 0.39 -1.9 1,024,730 + Pf6N2E2_874 0.39 +0.6 1,024,730 + Pf6N2E2_874 0.39 +0.4 1,024,730 + Pf6N2E2_874 0.39 -3.1 1,024,730 + Pf6N2E2_874 0.39 -0.2 1,024,730 + Pf6N2E2_874 0.39 -0.8 1,024,731 - Pf6N2E2_874 0.39 -2.2 1,024,731 - Pf6N2E2_874 0.39 -0.5 1,024,814 + Pf6N2E2_874 0.51 +0.9 1,024,815 - Pf6N2E2_874 0.51 +0.3 1,024,963 + Pf6N2E2_874 0.73 +0.5 1,024,984 + Pf6N2E2_874 0.76 -0.3 1,024,984 + Pf6N2E2_874 0.76 -2.1 1,024,985 - Pf6N2E2_874 0.76 +0.3 1,024,985 - Pf6N2E2_874 0.76 -1.0 1,025,069 + Pf6N2E2_874 0.89 -0.3 1,025,096 + +0.4 1,025,096 + +1.6 1,025,096 + -0.7 1,025,096 + -0.5 1,025,096 + -0.2 1,025,096 + +1.4 1,025,097 - +0.1 1,025,097 - +0.0 1,025,130 + +0.4 1,025,322 + Pf6N2E2_875 0.26 -1.2 1,025,330 + Pf6N2E2_875 0.28 +2.2 1,025,330 + Pf6N2E2_875 0.28 -1.3 1,025,331 - Pf6N2E2_875 0.28 -0.9 1,025,331 - Pf6N2E2_875 0.28 -1.7 1,025,331 - Pf6N2E2_875 0.28 -1.3 1,025,331 - Pf6N2E2_875 0.28 +0.6 1,025,365 - Pf6N2E2_875 0.36 +1.6 1,025,365 - Pf6N2E2_875 0.36 +0.5 1,025,432 + Pf6N2E2_875 0.51 -1.3 1,025,432 + Pf6N2E2_875 0.51 -0.9 1,025,432 + Pf6N2E2_875 0.51 -1.6 1,025,432 + Pf6N2E2_875 0.51 -0.8 1,025,432 + Pf6N2E2_875 0.51 +1.2 1,025,433 - Pf6N2E2_875 0.51 +0.4 1,025,433 - Pf6N2E2_875 0.51 -1.0 1,025,433 - Pf6N2E2_875 0.51 -0.4 1,025,433 - Pf6N2E2_875 0.51 -0.6 1,025,433 - Pf6N2E2_875 0.51 -1.1 1,025,433 - Pf6N2E2_875 0.51 -0.6 1,025,433 - Pf6N2E2_875 0.51 +0.1 1,025,433 - Pf6N2E2_875 0.51 -0.5 1,025,491 + Pf6N2E2_875 0.65 +2.4 1,025,492 - Pf6N2E2_875 0.65 +1.3 1,025,621 + -0.2 1,025,622 - -0.9 1,025,622 - -0.5 1,025,622 - +0.5 1,025,622 - -1.2 1,025,647 - +1.3 1,025,660 - +0.9 1,025,660 - +0.1 1,025,660 - -0.1 1,025,670 + +1.6 1,025,670 + -0.8 1,025,737 + Pf6N2E2_876 0.14 -1.1 1,025,761 - Pf6N2E2_876 0.18 -0.3 1,025,976 + Pf6N2E2_876 0.51 -0.8 1,026,092 - Pf6N2E2_876 0.69 -0.6 1,026,095 - Pf6N2E2_876 0.69 -0.6 1,026,095 - Pf6N2E2_876 0.69 -0.5 1,026,109 + Pf6N2E2_876 0.71 -1.0 1,026,110 - Pf6N2E2_876 0.71 -0.5 1,026,110 - Pf6N2E2_876 0.71 -0.2 1,026,110 - Pf6N2E2_876 0.71 -1.6 1,026,269 + -1.3 1,026,269 + +0.9 1,026,270 - +0.5 1,026,277 - -0.4 1,026,286 - +1.0 1,026,434 - Pf6N2E2_877 0.19 +0.6 1,026,472 + Pf6N2E2_877 0.25 +0.8 1,026,472 + Pf6N2E2_877 0.25 +1.9 1,026,472 + Pf6N2E2_877 0.25 -0.4 1,026,472 + Pf6N2E2_877 0.25 -0.7 1,026,473 - Pf6N2E2_877 0.25 +1.2 1,026,493 - Pf6N2E2_877 0.28 -0.7 1,026,493 - Pf6N2E2_877 0.28 +0.5 1,026,563 + Pf6N2E2_877 0.39 +0.8 1,026,563 + Pf6N2E2_877 0.39 +1.1 1,026,737 + Pf6N2E2_877 0.67 +1.1 1,026,738 - Pf6N2E2_877 0.67 +0.1 1,026,803 + Pf6N2E2_877 0.77 +0.3 1,026,803 + Pf6N2E2_877 0.77 -0.7 1,026,803 + Pf6N2E2_877 0.77 +1.0 1,026,803 + Pf6N2E2_877 0.77 -0.6 1,026,803 + Pf6N2E2_877 0.77 +0.0 1,026,803 + Pf6N2E2_877 0.77 -0.1 1,026,804 - Pf6N2E2_877 0.77 -0.2 1,026,804 - Pf6N2E2_877 0.77 -0.3 1,026,804 - Pf6N2E2_877 0.77 +0.6 1,026,804 - Pf6N2E2_877 0.77 -0.9 1,026,804 - Pf6N2E2_877 0.77 -1.3 1,026,804 - Pf6N2E2_877 0.77 -1.0 1,026,940 - +1.1 1,026,953 + +0.3 1,026,953 + -0.4 1,026,954 - +1.6 1,026,971 + +0.8 1,026,987 + +0.3 1,026,987 + -0.9 1,026,988 - +0.9 1,026,988 - -0.5 1,026,988 - +0.0 1,027,026 + +0.5 1,027,027 - +0.9 1,027,092 + +0.4 1,027,171 + -1.6 1,027,296 + Pf6N2E2_878 0.18 -0.1 1,027,296 + Pf6N2E2_878 0.18 +1.0 1,027,296 + Pf6N2E2_878 0.18 -2.2
Or see this region's nucleotide sequence