Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_76 and Pf6N2E2_77 are separated by 58 nucleotides Pf6N2E2_77 and Pf6N2E2_78 overlap by 4 nucleotides Pf6N2E2_78 and Pf6N2E2_6100 are separated by 168 nucleotides
Pf6N2E2_76 - Amidophosphoribosyltransferase (EC 2.4.2.14), at 117,987 to 119,492
_76
Pf6N2E2_77 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48), at 119,551 to 120,762
_77
Pf6N2E2_78 - Oxidoreductase, short chain dehydrogenase/reductase family, at 120,759 to 121,532
_78
Pf6N2E2_6100 - tRNA-Val-TAC, at 121,701 to 121,773
_6100
Position (kb)
119
120
121 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 118.680 kb on - strand, within Pf6N2E2_76 at 119.481 kb on + strand at 119.482 kb on - strand at 119.482 kb on - strand at 119.676 kb on + strand, within Pf6N2E2_77 at 119.687 kb on - strand, within Pf6N2E2_77 at 119.687 kb on - strand, within Pf6N2E2_77 at 119.687 kb on - strand, within Pf6N2E2_77 at 119.944 kb on + strand, within Pf6N2E2_77 at 119.945 kb on - strand, within Pf6N2E2_77 at 119.974 kb on + strand, within Pf6N2E2_77 at 120.024 kb on + strand, within Pf6N2E2_77 at 120.439 kb on + strand, within Pf6N2E2_77 at 121.092 kb on + strand, within Pf6N2E2_78 at 121.159 kb on - strand, within Pf6N2E2_78 at 121.236 kb on + strand, within Pf6N2E2_78 at 121.236 kb on + strand, within Pf6N2E2_78 at 121.237 kb on - strand, within Pf6N2E2_78 at 121.237 kb on - strand, within Pf6N2E2_78 at 121.412 kb on - strand, within Pf6N2E2_78 at 121.421 kb on - strand, within Pf6N2E2_78 at 121.524 kb on + strand at 121.524 kb on + strand at 121.525 kb on - strand at 121.525 kb on - strand at 121.525 kb on - strand at 121.572 kb on + strand at 121.572 kb on + strand at 121.573 kb on - strand at 121.598 kb on + strand at 121.599 kb on - strand at 121.658 kb on - strand at 121.734 kb on + strand, within Pf6N2E2_6100 at 121.734 kb on + strand, within Pf6N2E2_6100
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 118,680 - Pf6N2E2_76 0.46 -1.5 119,481 + -1.1 119,482 - -0.9 119,482 - -2.0 119,676 + Pf6N2E2_77 0.10 -3.4 119,687 - Pf6N2E2_77 0.11 -2.6 119,687 - Pf6N2E2_77 0.11 -2.0 119,687 - Pf6N2E2_77 0.11 -3.2 119,944 + Pf6N2E2_77 0.32 -0.1 119,945 - Pf6N2E2_77 0.33 -1.8 119,974 + Pf6N2E2_77 0.35 -2.2 120,024 + Pf6N2E2_77 0.39 -0.6 120,439 + Pf6N2E2_77 0.73 -3.1 121,092 + Pf6N2E2_78 0.43 -0.6 121,159 - Pf6N2E2_78 0.52 -1.2 121,236 + Pf6N2E2_78 0.62 -2.1 121,236 + Pf6N2E2_78 0.62 +0.4 121,237 - Pf6N2E2_78 0.62 -0.2 121,237 - Pf6N2E2_78 0.62 -0.1 121,412 - Pf6N2E2_78 0.84 +1.9 121,421 - Pf6N2E2_78 0.86 +0.6 121,524 + +1.1 121,524 + -1.2 121,525 - +0.2 121,525 - +0.0 121,525 - -1.1 121,572 + -0.2 121,572 + -0.5 121,573 - +0.6 121,598 + +1.9 121,599 - +0.1 121,658 - +1.7 121,734 + Pf6N2E2_6100 0.45 -1.2 121,734 + Pf6N2E2_6100 0.45 +0.5
Or see this region's nucleotide sequence