Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_77

Experiment: D-Glucosamine Hydrochloride (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_76 and Pf6N2E2_77 are separated by 58 nucleotidesPf6N2E2_77 and Pf6N2E2_78 overlap by 4 nucleotidesPf6N2E2_78 and Pf6N2E2_6100 are separated by 168 nucleotides Pf6N2E2_76 - Amidophosphoribosyltransferase (EC 2.4.2.14), at 117,987 to 119,492 _76 Pf6N2E2_77 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48), at 119,551 to 120,762 _77 Pf6N2E2_78 - Oxidoreductase, short chain dehydrogenase/reductase family, at 120,759 to 121,532 _78 Pf6N2E2_6100 - tRNA-Val-TAC, at 121,701 to 121,773 _6100 Position (kb) 119 120 121Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 118.680 kb on - strand, within Pf6N2E2_76at 119.481 kb on + strandat 119.482 kb on - strandat 119.482 kb on - strandat 119.676 kb on + strand, within Pf6N2E2_77at 119.687 kb on - strand, within Pf6N2E2_77at 119.687 kb on - strand, within Pf6N2E2_77at 119.687 kb on - strand, within Pf6N2E2_77at 119.944 kb on + strand, within Pf6N2E2_77at 119.945 kb on - strand, within Pf6N2E2_77at 119.974 kb on + strand, within Pf6N2E2_77at 120.024 kb on + strand, within Pf6N2E2_77at 120.439 kb on + strand, within Pf6N2E2_77at 121.092 kb on + strand, within Pf6N2E2_78at 121.159 kb on - strand, within Pf6N2E2_78at 121.236 kb on + strand, within Pf6N2E2_78at 121.236 kb on + strand, within Pf6N2E2_78at 121.237 kb on - strand, within Pf6N2E2_78at 121.237 kb on - strand, within Pf6N2E2_78at 121.412 kb on - strand, within Pf6N2E2_78at 121.421 kb on - strand, within Pf6N2E2_78at 121.524 kb on + strandat 121.524 kb on + strandat 121.525 kb on - strandat 121.525 kb on - strandat 121.525 kb on - strandat 121.572 kb on + strandat 121.572 kb on + strandat 121.573 kb on - strandat 121.598 kb on + strandat 121.599 kb on - strandat 121.658 kb on - strandat 121.734 kb on + strand, within Pf6N2E2_6100at 121.734 kb on + strand, within Pf6N2E2_6100

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C)
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118,680 - Pf6N2E2_76 0.46 -1.5
119,481 + -1.1
119,482 - -0.9
119,482 - -2.0
119,676 + Pf6N2E2_77 0.10 -3.4
119,687 - Pf6N2E2_77 0.11 -2.6
119,687 - Pf6N2E2_77 0.11 -2.0
119,687 - Pf6N2E2_77 0.11 -3.2
119,944 + Pf6N2E2_77 0.32 -0.1
119,945 - Pf6N2E2_77 0.33 -1.8
119,974 + Pf6N2E2_77 0.35 -2.2
120,024 + Pf6N2E2_77 0.39 -0.6
120,439 + Pf6N2E2_77 0.73 -3.1
121,092 + Pf6N2E2_78 0.43 -0.6
121,159 - Pf6N2E2_78 0.52 -1.2
121,236 + Pf6N2E2_78 0.62 -2.1
121,236 + Pf6N2E2_78 0.62 +0.4
121,237 - Pf6N2E2_78 0.62 -0.2
121,237 - Pf6N2E2_78 0.62 -0.1
121,412 - Pf6N2E2_78 0.84 +1.9
121,421 - Pf6N2E2_78 0.86 +0.6
121,524 + +1.1
121,524 + -1.2
121,525 - +0.2
121,525 - +0.0
121,525 - -1.1
121,572 + -0.2
121,572 + -0.5
121,573 - +0.6
121,598 + +1.9
121,599 - +0.1
121,658 - +1.7
121,734 + Pf6N2E2_6100 0.45 -1.2
121,734 + Pf6N2E2_6100 0.45 +0.5

Or see this region's nucleotide sequence