Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_694

Experiment: D-Glucosamine Hydrochloride (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_692 and Pf6N2E2_693 are separated by 274 nucleotidesPf6N2E2_693 and Pf6N2E2_694 overlap by 6 nucleotidesPf6N2E2_694 and Pf6N2E2_695 are separated by 99 nucleotides Pf6N2E2_692 - Glucarate dehydratase (EC 4.2.1.40), at 798,439 to 799,713 _692 Pf6N2E2_693 - hypothetical protein, at 799,988 to 800,125 _693 Pf6N2E2_694 - Outer membrane protein (porin), at 800,120 to 801,415 _694 Pf6N2E2_695 - Nitrite reductase associated c-type cytochorome NirN, at 801,515 to 803,011 _695 Position (kb) 800 801 802Strain fitness (log2 ratio) -1 0 1 2 3at 799.168 kb on + strand, within Pf6N2E2_692at 799.168 kb on + strand, within Pf6N2E2_692at 799.927 kb on - strandat 799.952 kb on + strandat 799.952 kb on + strandat 799.953 kb on - strandat 799.953 kb on - strandat 799.953 kb on - strandat 799.990 kb on - strandat 799.990 kb on - strandat 799.990 kb on - strandat 800.055 kb on - strand, within Pf6N2E2_693at 800.055 kb on - strand, within Pf6N2E2_693at 800.055 kb on - strand, within Pf6N2E2_693at 800.132 kb on - strandat 800.169 kb on + strandat 800.169 kb on + strandat 800.169 kb on + strandat 800.169 kb on + strandat 800.169 kb on + strandat 800.170 kb on - strandat 800.170 kb on - strandat 800.170 kb on - strandat 800.170 kb on - strandat 800.222 kb on + strandat 800.258 kb on + strand, within Pf6N2E2_694at 800.273 kb on + strand, within Pf6N2E2_694at 800.274 kb on - strand, within Pf6N2E2_694at 800.309 kb on + strand, within Pf6N2E2_694at 800.354 kb on + strand, within Pf6N2E2_694at 800.354 kb on + strand, within Pf6N2E2_694at 800.355 kb on - strand, within Pf6N2E2_694at 800.355 kb on - strand, within Pf6N2E2_694at 800.355 kb on - strand, within Pf6N2E2_694at 800.355 kb on - strand, within Pf6N2E2_694at 800.480 kb on + strand, within Pf6N2E2_694at 800.481 kb on - strand, within Pf6N2E2_694at 800.481 kb on - strand, within Pf6N2E2_694at 800.481 kb on - strand, within Pf6N2E2_694at 800.481 kb on - strand, within Pf6N2E2_694at 800.814 kb on - strand, within Pf6N2E2_694at 800.843 kb on + strand, within Pf6N2E2_694at 800.843 kb on + strand, within Pf6N2E2_694at 800.844 kb on - strand, within Pf6N2E2_694at 800.844 kb on - strand, within Pf6N2E2_694at 800.844 kb on - strand, within Pf6N2E2_694at 800.864 kb on + strand, within Pf6N2E2_694at 800.925 kb on - strand, within Pf6N2E2_694at 801.051 kb on - strand, within Pf6N2E2_694at 801.068 kb on + strand, within Pf6N2E2_694at 801.068 kb on + strand, within Pf6N2E2_694at 801.068 kb on + strand, within Pf6N2E2_694at 801.485 kb on + strandat 801.561 kb on + strandat 801.561 kb on + strandat 801.562 kb on - strandat 801.562 kb on - strandat 801.562 kb on - strandat 801.807 kb on + strand, within Pf6N2E2_695at 801.957 kb on + strand, within Pf6N2E2_695at 801.958 kb on - strand, within Pf6N2E2_695at 802.124 kb on - strand, within Pf6N2E2_695at 802.224 kb on + strand, within Pf6N2E2_695

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C)
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799,168 + Pf6N2E2_692 0.57 +1.4
799,168 + Pf6N2E2_692 0.57 +0.6
799,927 - +1.2
799,952 + +2.5
799,952 + +1.7
799,953 - +1.3
799,953 - +1.5
799,953 - +1.6
799,990 - +2.0
799,990 - +1.6
799,990 - +2.1
800,055 - Pf6N2E2_693 0.49 +2.3
800,055 - Pf6N2E2_693 0.49 +2.1
800,055 - Pf6N2E2_693 0.49 +1.7
800,132 - +1.6
800,169 + +1.1
800,169 + +1.2
800,169 + +1.2
800,169 + +2.4
800,169 + +2.5
800,170 - +1.8
800,170 - +2.2
800,170 - +2.7
800,170 - +1.0
800,222 + +0.3
800,258 + Pf6N2E2_694 0.11 +0.4
800,273 + Pf6N2E2_694 0.12 +1.6
800,274 - Pf6N2E2_694 0.12 +3.1
800,309 + Pf6N2E2_694 0.15 +1.9
800,354 + Pf6N2E2_694 0.18 +0.9
800,354 + Pf6N2E2_694 0.18 +1.1
800,355 - Pf6N2E2_694 0.18 +2.6
800,355 - Pf6N2E2_694 0.18 +0.1
800,355 - Pf6N2E2_694 0.18 +1.1
800,355 - Pf6N2E2_694 0.18 +2.4
800,480 + Pf6N2E2_694 0.28 +0.7
800,481 - Pf6N2E2_694 0.28 +2.6
800,481 - Pf6N2E2_694 0.28 +2.5
800,481 - Pf6N2E2_694 0.28 +2.1
800,481 - Pf6N2E2_694 0.28 +3.0
800,814 - Pf6N2E2_694 0.54 +2.5
800,843 + Pf6N2E2_694 0.56 +1.4
800,843 + Pf6N2E2_694 0.56 +1.6
800,844 - Pf6N2E2_694 0.56 +1.2
800,844 - Pf6N2E2_694 0.56 +1.9
800,844 - Pf6N2E2_694 0.56 +1.5
800,864 + Pf6N2E2_694 0.57 +0.6
800,925 - Pf6N2E2_694 0.62 +1.7
801,051 - Pf6N2E2_694 0.72 +1.4
801,068 + Pf6N2E2_694 0.73 +1.5
801,068 + Pf6N2E2_694 0.73 +1.2
801,068 + Pf6N2E2_694 0.73 +0.1
801,485 + -0.1
801,561 + -1.5
801,561 + +0.3
801,562 - -0.3
801,562 - +1.0
801,562 - +0.5
801,807 + Pf6N2E2_695 0.20 -0.1
801,957 + Pf6N2E2_695 0.30 -1.1
801,958 - Pf6N2E2_695 0.30 +0.4
802,124 - Pf6N2E2_695 0.41 +1.5
802,224 + Pf6N2E2_695 0.47 -0.3

Or see this region's nucleotide sequence