Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_692 and Pf6N2E2_693 are separated by 274 nucleotides Pf6N2E2_693 and Pf6N2E2_694 overlap by 6 nucleotides Pf6N2E2_694 and Pf6N2E2_695 are separated by 99 nucleotides
Pf6N2E2_692 - Glucarate dehydratase (EC 4.2.1.40), at 798,439 to 799,713
_692
Pf6N2E2_693 - hypothetical protein, at 799,988 to 800,125
_693
Pf6N2E2_694 - Outer membrane protein (porin), at 800,120 to 801,415
_694
Pf6N2E2_695 - Nitrite reductase associated c-type cytochorome NirN, at 801,515 to 803,011
_695
Position (kb) 800
801
802 Strain fitness (log2 ratio) -1
0
1
2
3 at 799.168 kb on + strand, within Pf6N2E2_692 at 799.168 kb on + strand, within Pf6N2E2_692 at 799.927 kb on - strand at 799.952 kb on + strand at 799.952 kb on + strand at 799.953 kb on - strand at 799.953 kb on - strand at 799.953 kb on - strand at 799.990 kb on - strand at 799.990 kb on - strand at 799.990 kb on - strand at 800.055 kb on - strand, within Pf6N2E2_693 at 800.055 kb on - strand, within Pf6N2E2_693 at 800.055 kb on - strand, within Pf6N2E2_693 at 800.132 kb on - strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.222 kb on + strand at 800.258 kb on + strand, within Pf6N2E2_694 at 800.273 kb on + strand, within Pf6N2E2_694 at 800.274 kb on - strand, within Pf6N2E2_694 at 800.309 kb on + strand, within Pf6N2E2_694 at 800.354 kb on + strand, within Pf6N2E2_694 at 800.354 kb on + strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.480 kb on + strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.814 kb on - strand, within Pf6N2E2_694 at 800.843 kb on + strand, within Pf6N2E2_694 at 800.843 kb on + strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.864 kb on + strand, within Pf6N2E2_694 at 800.925 kb on - strand, within Pf6N2E2_694 at 801.051 kb on - strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.485 kb on + strand at 801.561 kb on + strand at 801.561 kb on + strand at 801.562 kb on - strand at 801.562 kb on - strand at 801.562 kb on - strand at 801.807 kb on + strand, within Pf6N2E2_695 at 801.957 kb on + strand, within Pf6N2E2_695 at 801.958 kb on - strand, within Pf6N2E2_695 at 802.124 kb on - strand, within Pf6N2E2_695 at 802.224 kb on + strand, within Pf6N2E2_695
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 799,168 + Pf6N2E2_692 0.57 +1.4 799,168 + Pf6N2E2_692 0.57 +0.6 799,927 - +1.2 799,952 + +2.5 799,952 + +1.7 799,953 - +1.3 799,953 - +1.5 799,953 - +1.6 799,990 - +2.0 799,990 - +1.6 799,990 - +2.1 800,055 - Pf6N2E2_693 0.49 +2.3 800,055 - Pf6N2E2_693 0.49 +2.1 800,055 - Pf6N2E2_693 0.49 +1.7 800,132 - +1.6 800,169 + +1.1 800,169 + +1.2 800,169 + +1.2 800,169 + +2.4 800,169 + +2.5 800,170 - +1.8 800,170 - +2.2 800,170 - +2.7 800,170 - +1.0 800,222 + +0.3 800,258 + Pf6N2E2_694 0.11 +0.4 800,273 + Pf6N2E2_694 0.12 +1.6 800,274 - Pf6N2E2_694 0.12 +3.1 800,309 + Pf6N2E2_694 0.15 +1.9 800,354 + Pf6N2E2_694 0.18 +0.9 800,354 + Pf6N2E2_694 0.18 +1.1 800,355 - Pf6N2E2_694 0.18 +2.6 800,355 - Pf6N2E2_694 0.18 +0.1 800,355 - Pf6N2E2_694 0.18 +1.1 800,355 - Pf6N2E2_694 0.18 +2.4 800,480 + Pf6N2E2_694 0.28 +0.7 800,481 - Pf6N2E2_694 0.28 +2.6 800,481 - Pf6N2E2_694 0.28 +2.5 800,481 - Pf6N2E2_694 0.28 +2.1 800,481 - Pf6N2E2_694 0.28 +3.0 800,814 - Pf6N2E2_694 0.54 +2.5 800,843 + Pf6N2E2_694 0.56 +1.4 800,843 + Pf6N2E2_694 0.56 +1.6 800,844 - Pf6N2E2_694 0.56 +1.2 800,844 - Pf6N2E2_694 0.56 +1.9 800,844 - Pf6N2E2_694 0.56 +1.5 800,864 + Pf6N2E2_694 0.57 +0.6 800,925 - Pf6N2E2_694 0.62 +1.7 801,051 - Pf6N2E2_694 0.72 +1.4 801,068 + Pf6N2E2_694 0.73 +1.5 801,068 + Pf6N2E2_694 0.73 +1.2 801,068 + Pf6N2E2_694 0.73 +0.1 801,485 + -0.1 801,561 + -1.5 801,561 + +0.3 801,562 - -0.3 801,562 - +1.0 801,562 - +0.5 801,807 + Pf6N2E2_695 0.20 -0.1 801,957 + Pf6N2E2_695 0.30 -1.1 801,958 - Pf6N2E2_695 0.30 +0.4 802,124 - Pf6N2E2_695 0.41 +1.5 802,224 + Pf6N2E2_695 0.47 -0.3
Or see this region's nucleotide sequence