Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_64 and Pf6N2E2_65 are separated by 138 nucleotides Pf6N2E2_65 and Pf6N2E2_66 are separated by 66 nucleotides Pf6N2E2_66 and Pf6N2E2_67 are separated by 54 nucleotides
Pf6N2E2_64 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 104,440 to 105,084
_64
Pf6N2E2_65 - SAM-dependent methyltransferase YafE (UbiE paralog), at 105,223 to 105,987
_65
Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136
_66
Pf6N2E2_67 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 107,191 to 108,303
_67
Position (kb)
106
107
108 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 105.092 kb on + strand at 105.251 kb on + strand at 105.252 kb on - strand at 105.252 kb on - strand at 105.283 kb on + strand at 105.329 kb on + strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.437 kb on - strand, within Pf6N2E2_65 at 105.697 kb on + strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.801 kb on + strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.855 kb on + strand, within Pf6N2E2_65 at 105.954 kb on + strand at 105.955 kb on - strand at 106.013 kb on - strand at 106.013 kb on - strand at 106.147 kb on + strand at 106.147 kb on + strand at 106.148 kb on - strand at 106.148 kb on - strand at 106.470 kb on + strand, within Pf6N2E2_66 at 106.470 kb on + strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.543 kb on + strand, within Pf6N2E2_66 at 106.637 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.975 kb on + strand, within Pf6N2E2_66 at 107.076 kb on + strand at 107.111 kb on + strand at 107.177 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 105,092 + -3.2 105,251 + -0.9 105,252 - -1.2 105,252 - -0.8 105,283 + +0.7 105,329 + Pf6N2E2_65 0.14 -1.4 105,330 - Pf6N2E2_65 0.14 +0.9 105,330 - Pf6N2E2_65 0.14 +0.5 105,330 - Pf6N2E2_65 0.14 +0.4 105,330 - Pf6N2E2_65 0.14 +0.3 105,330 - Pf6N2E2_65 0.14 +1.4 105,436 + Pf6N2E2_65 0.28 +0.5 105,436 + Pf6N2E2_65 0.28 -1.2 105,437 - Pf6N2E2_65 0.28 +0.8 105,697 + Pf6N2E2_65 0.62 -0.2 105,698 - Pf6N2E2_65 0.62 +0.6 105,698 - Pf6N2E2_65 0.62 -0.5 105,801 + Pf6N2E2_65 0.76 -1.5 105,802 - Pf6N2E2_65 0.76 -0.3 105,802 - Pf6N2E2_65 0.76 -0.6 105,829 + Pf6N2E2_65 0.79 -1.1 105,829 + Pf6N2E2_65 0.79 -0.6 105,855 + Pf6N2E2_65 0.83 -1.6 105,954 + -1.3 105,955 - +0.9 106,013 - -1.5 106,013 - -0.6 106,147 + -3.6 106,147 + -1.5 106,148 - -2.7 106,148 - -2.2 106,470 + Pf6N2E2_66 0.38 -1.5 106,470 + Pf6N2E2_66 0.38 -3.3 106,475 - Pf6N2E2_66 0.39 -2.3 106,475 - Pf6N2E2_66 0.39 -3.1 106,475 - Pf6N2E2_66 0.39 -1.4 106,529 - Pf6N2E2_66 0.44 -2.2 106,529 - Pf6N2E2_66 0.44 -0.4 106,543 + Pf6N2E2_66 0.45 -3.6 106,637 + Pf6N2E2_66 0.54 -1.2 106,717 + Pf6N2E2_66 0.61 -1.6 106,717 + Pf6N2E2_66 0.61 -3.2 106,717 + Pf6N2E2_66 0.61 -0.6 106,717 + Pf6N2E2_66 0.61 -1.2 106,717 + Pf6N2E2_66 0.61 -2.5 106,717 + Pf6N2E2_66 0.61 -1.1 106,718 - Pf6N2E2_66 0.61 -2.8 106,718 - Pf6N2E2_66 0.61 -4.1 106,718 - Pf6N2E2_66 0.61 -1.4 106,718 - Pf6N2E2_66 0.61 -2.0 106,718 - Pf6N2E2_66 0.61 -1.4 106,718 - Pf6N2E2_66 0.61 -1.2 106,718 - Pf6N2E2_66 0.61 -1.5 106,882 + Pf6N2E2_66 0.76 -2.6 106,882 + Pf6N2E2_66 0.76 -2.7 106,883 - Pf6N2E2_66 0.77 -0.9 106,883 - Pf6N2E2_66 0.77 -1.1 106,883 - Pf6N2E2_66 0.77 -1.5 106,883 - Pf6N2E2_66 0.77 -0.5 106,883 - Pf6N2E2_66 0.77 -3.3 106,975 + Pf6N2E2_66 0.85 -3.2 107,076 + -2.6 107,111 + +1.7 107,177 + +0.1
Or see this region's nucleotide sequence