Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_66

Experiment: D-Glucosamine Hydrochloride (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_64 and Pf6N2E2_65 are separated by 138 nucleotidesPf6N2E2_65 and Pf6N2E2_66 are separated by 66 nucleotidesPf6N2E2_66 and Pf6N2E2_67 are separated by 54 nucleotides Pf6N2E2_64 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 104,440 to 105,084 _64 Pf6N2E2_65 - SAM-dependent methyltransferase YafE (UbiE paralog), at 105,223 to 105,987 _65 Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136 _66 Pf6N2E2_67 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 107,191 to 108,303 _67 Position (kb) 106 107 108Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 105.092 kb on + strandat 105.251 kb on + strandat 105.252 kb on - strandat 105.252 kb on - strandat 105.283 kb on + strandat 105.329 kb on + strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.436 kb on + strand, within Pf6N2E2_65at 105.436 kb on + strand, within Pf6N2E2_65at 105.437 kb on - strand, within Pf6N2E2_65at 105.697 kb on + strand, within Pf6N2E2_65at 105.698 kb on - strand, within Pf6N2E2_65at 105.698 kb on - strand, within Pf6N2E2_65at 105.801 kb on + strand, within Pf6N2E2_65at 105.802 kb on - strand, within Pf6N2E2_65at 105.802 kb on - strand, within Pf6N2E2_65at 105.829 kb on + strand, within Pf6N2E2_65at 105.829 kb on + strand, within Pf6N2E2_65at 105.855 kb on + strand, within Pf6N2E2_65at 105.954 kb on + strandat 105.955 kb on - strandat 106.013 kb on - strandat 106.013 kb on - strandat 106.147 kb on + strandat 106.147 kb on + strandat 106.148 kb on - strandat 106.148 kb on - strandat 106.470 kb on + strand, within Pf6N2E2_66at 106.470 kb on + strand, within Pf6N2E2_66at 106.475 kb on - strand, within Pf6N2E2_66at 106.475 kb on - strand, within Pf6N2E2_66at 106.475 kb on - strand, within Pf6N2E2_66at 106.529 kb on - strand, within Pf6N2E2_66at 106.529 kb on - strand, within Pf6N2E2_66at 106.543 kb on + strand, within Pf6N2E2_66at 106.637 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.882 kb on + strand, within Pf6N2E2_66at 106.882 kb on + strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.975 kb on + strand, within Pf6N2E2_66at 107.076 kb on + strandat 107.111 kb on + strandat 107.177 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C)
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105,092 + -3.2
105,251 + -0.9
105,252 - -1.2
105,252 - -0.8
105,283 + +0.7
105,329 + Pf6N2E2_65 0.14 -1.4
105,330 - Pf6N2E2_65 0.14 +0.9
105,330 - Pf6N2E2_65 0.14 +0.5
105,330 - Pf6N2E2_65 0.14 +0.4
105,330 - Pf6N2E2_65 0.14 +0.3
105,330 - Pf6N2E2_65 0.14 +1.4
105,436 + Pf6N2E2_65 0.28 +0.5
105,436 + Pf6N2E2_65 0.28 -1.2
105,437 - Pf6N2E2_65 0.28 +0.8
105,697 + Pf6N2E2_65 0.62 -0.2
105,698 - Pf6N2E2_65 0.62 +0.6
105,698 - Pf6N2E2_65 0.62 -0.5
105,801 + Pf6N2E2_65 0.76 -1.5
105,802 - Pf6N2E2_65 0.76 -0.3
105,802 - Pf6N2E2_65 0.76 -0.6
105,829 + Pf6N2E2_65 0.79 -1.1
105,829 + Pf6N2E2_65 0.79 -0.6
105,855 + Pf6N2E2_65 0.83 -1.6
105,954 + -1.3
105,955 - +0.9
106,013 - -1.5
106,013 - -0.6
106,147 + -3.6
106,147 + -1.5
106,148 - -2.7
106,148 - -2.2
106,470 + Pf6N2E2_66 0.38 -1.5
106,470 + Pf6N2E2_66 0.38 -3.3
106,475 - Pf6N2E2_66 0.39 -2.3
106,475 - Pf6N2E2_66 0.39 -3.1
106,475 - Pf6N2E2_66 0.39 -1.4
106,529 - Pf6N2E2_66 0.44 -2.2
106,529 - Pf6N2E2_66 0.44 -0.4
106,543 + Pf6N2E2_66 0.45 -3.6
106,637 + Pf6N2E2_66 0.54 -1.2
106,717 + Pf6N2E2_66 0.61 -1.6
106,717 + Pf6N2E2_66 0.61 -3.2
106,717 + Pf6N2E2_66 0.61 -0.6
106,717 + Pf6N2E2_66 0.61 -1.2
106,717 + Pf6N2E2_66 0.61 -2.5
106,717 + Pf6N2E2_66 0.61 -1.1
106,718 - Pf6N2E2_66 0.61 -2.8
106,718 - Pf6N2E2_66 0.61 -4.1
106,718 - Pf6N2E2_66 0.61 -1.4
106,718 - Pf6N2E2_66 0.61 -2.0
106,718 - Pf6N2E2_66 0.61 -1.4
106,718 - Pf6N2E2_66 0.61 -1.2
106,718 - Pf6N2E2_66 0.61 -1.5
106,882 + Pf6N2E2_66 0.76 -2.6
106,882 + Pf6N2E2_66 0.76 -2.7
106,883 - Pf6N2E2_66 0.77 -0.9
106,883 - Pf6N2E2_66 0.77 -1.1
106,883 - Pf6N2E2_66 0.77 -1.5
106,883 - Pf6N2E2_66 0.77 -0.5
106,883 - Pf6N2E2_66 0.77 -3.3
106,975 + Pf6N2E2_66 0.85 -3.2
107,076 + -2.6
107,111 + +1.7
107,177 + +0.1

Or see this region's nucleotide sequence