Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_629 and Pf6N2E2_630 overlap by 8 nucleotides Pf6N2E2_630 and Pf6N2E2_631 are separated by 63 nucleotides
Pf6N2E2_629 - 2-ketogluconate kinase (EC 2.7.1.13), at 716,617 to 717,591
_629
Pf6N2E2_630 - Epimerase KguE, at 717,584 to 718,366
_630
Pf6N2E2_631 - 2-ketogluconate utilization repressor PtxS, at 718,430 to 719,455
_631
Position (kb) 717
718
719 Strain fitness (log2 ratio) -2
-1
0
1 at 716.888 kb on + strand, within Pf6N2E2_629 at 716.889 kb on - strand, within Pf6N2E2_629 at 716.889 kb on - strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.698 kb on + strand, within Pf6N2E2_630 at 717.699 kb on - strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 718.071 kb on - strand, within Pf6N2E2_630 at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.371 kb on - strand at 718.371 kb on - strand at 718.373 kb on + strand at 718.373 kb on + strand at 718.634 kb on + strand, within Pf6N2E2_631 at 718.634 kb on + strand, within Pf6N2E2_631 at 718.635 kb on - strand, within Pf6N2E2_631 at 718.635 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.152 kb on + strand, within Pf6N2E2_631 at 719.152 kb on + strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 716,888 + Pf6N2E2_629 0.28 +0.4 716,889 - Pf6N2E2_629 0.28 +1.6 716,889 - Pf6N2E2_629 0.28 +1.5 717,272 + Pf6N2E2_629 0.67 -0.8 717,272 + Pf6N2E2_629 0.67 -2.2 717,272 + Pf6N2E2_629 0.67 +0.6 717,698 + Pf6N2E2_630 0.15 -1.0 717,699 - Pf6N2E2_630 0.15 -1.0 717,813 + Pf6N2E2_630 0.29 -1.2 717,813 + Pf6N2E2_630 0.29 -1.2 717,813 + Pf6N2E2_630 0.29 -0.8 717,814 - Pf6N2E2_630 0.29 +0.1 717,814 - Pf6N2E2_630 0.29 -0.2 718,071 - Pf6N2E2_630 0.62 -1.0 718,370 + +0.4 718,370 + -0.9 718,370 + +1.3 718,370 + -0.8 718,370 + +1.0 718,371 - -0.2 718,371 - +0.5 718,373 + -0.6 718,373 + -0.6 718,634 + Pf6N2E2_631 0.20 -0.5 718,634 + Pf6N2E2_631 0.20 +0.9 718,635 - Pf6N2E2_631 0.20 +1.1 718,635 - Pf6N2E2_631 0.20 +1.1 719,083 - Pf6N2E2_631 0.64 -0.4 719,083 - Pf6N2E2_631 0.64 +0.2 719,083 - Pf6N2E2_631 0.64 -1.4 719,152 + Pf6N2E2_631 0.70 +0.6 719,152 + Pf6N2E2_631 0.70 -0.6 719,153 - Pf6N2E2_631 0.70 +0.2 719,153 - Pf6N2E2_631 0.70 +1.3 719,153 - Pf6N2E2_631 0.70 +1.4
Or see this region's nucleotide sequence